Rhizobial diversity associated with the spontaneous legume Genista saharae in the northeastern Algerian Sahara
Genista saharae is an indigenous shrub legume that spontaneously grows in the northeastern Algerian Sahara. It is known for efficient dune fixation and soil preservation against desertification, due to its drought tolerance and its contribution to sustainable nitrogen resources implemented by biolog...
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| Veröffentlicht in: | Symbiosis (Philadelphia, Pa.) Pa.), 2017-02, Vol.71 (2), p.111-120 |
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| creator | Chaïch, Khaled Bekki, Abdelkader Bouras, Noureddine Holtz, Michael D. Soussou, Souhir Mauré, Lucette Brunel, Brigitte de Lajudie, Philippe Cleyet-Marel, Jean-Claude |
| description | Genista saharae
is an indigenous shrub legume that spontaneously grows in the northeastern Algerian Sahara. It is known for efficient dune fixation and soil preservation against desertification, due to its drought tolerance and its contribution to sustainable nitrogen resources implemented by biological N
2
-fixation. In this study, the root nodule bacteria of
G. saharae
were investigated using phenotypic and phylogenetic characterization. A total of 57 rhizobial strains were isolated from nodules from several sites in the hyper-arid region of Metlili and Taibet (east Septentrional Sahara). They all nodulate
G. saharae
species but they differed in their symbiotic efficiency and effectiveness. The genetic diversity was assessed by sequencing three housekeeping genes (
atpD
,
recA
and 16S rRNA). The majority of isolates (81 %) belonged to the genus
Ensifer
(previously
Sinorhizobium
), represented mainly by the species
Ensifer meliloti
. The next most abundant genera were
Neorhizobium
(17 %) with 3 different species:
N. alkalisoli
,
N. galegae
and
N. huautlense
and
Mesorhizobium
(1.75 %) represented by the species
M. camelthorni
. Most of the isolated strains tolerated up to 4 % (w/v) NaCl and grew at 45 °C. This study is the first report on the characterization of
G. saharae
microsymbionts in the Algerian Sahara. |
| doi_str_mv | 10.1007/s13199-016-0414-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_01602774v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1880787244</sourcerecordid><originalsourceid>FETCH-LOGICAL-c350t-482634c396059a90ec81fb6f7de6fa89f589359552ab5cb7b6cc64029d7893793</originalsourceid><addsrcrecordid>eNp1kU9rGzEQxUVpoK6TD5CboKceNpVW_48mtEnAEGiTs5hda70KG8mV5BTn00f2ltJLTgMzv_cY3kPokpIrSoj6limjxjSEyoZwypvDB7SgWulGSSk-ogVhjDeCUv4Jfc75iRBGJZMLFH6O_jV2Hia88S8uZV8OGHKOvYfiNviPLyMuo8N5F0OB4OI-48lt988O37jgcwGcYYQEDvtwIkNMdUAuLgW8mrYueQj41wk6R2cDTNld_J1L9Pjj-8P1bbO-v7m7Xq2bnglSGq5byXjPjCTCgCGu13To5KA2Tg6gzSC0YcII0UIn-k51su8lJ63ZqHpQhi3R19l3hMnukn-GdLARvL1dre1xV4MirVL8hVb2y8zuUvy9d7nYp7hPob5nqdZEadVyXik6U32KOSc3_LOlxB4rsHMFR2d7rMAeqqadNbmyoQbxn_O7ojcvG4oh</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1880787244</pqid></control><display><type>article</type><title>Rhizobial diversity associated with the spontaneous legume Genista saharae in the northeastern Algerian Sahara</title><source>SpringerLink Journals - AutoHoldings</source><creator>Chaïch, Khaled ; Bekki, Abdelkader ; Bouras, Noureddine ; Holtz, Michael D. ; Soussou, Souhir ; Mauré, Lucette ; Brunel, Brigitte ; de Lajudie, Philippe ; Cleyet-Marel, Jean-Claude</creator><creatorcontrib>Chaïch, Khaled ; Bekki, Abdelkader ; Bouras, Noureddine ; Holtz, Michael D. ; Soussou, Souhir ; Mauré, Lucette ; Brunel, Brigitte ; de Lajudie, Philippe ; Cleyet-Marel, Jean-Claude</creatorcontrib><description>Genista saharae
is an indigenous shrub legume that spontaneously grows in the northeastern Algerian Sahara. It is known for efficient dune fixation and soil preservation against desertification, due to its drought tolerance and its contribution to sustainable nitrogen resources implemented by biological N
2
-fixation. In this study, the root nodule bacteria of
G. saharae
were investigated using phenotypic and phylogenetic characterization. A total of 57 rhizobial strains were isolated from nodules from several sites in the hyper-arid region of Metlili and Taibet (east Septentrional Sahara). They all nodulate
G. saharae
species but they differed in their symbiotic efficiency and effectiveness. The genetic diversity was assessed by sequencing three housekeeping genes (
atpD
,
recA
and 16S rRNA). The majority of isolates (81 %) belonged to the genus
Ensifer
(previously
Sinorhizobium
), represented mainly by the species
Ensifer meliloti
. The next most abundant genera were
Neorhizobium
(17 %) with 3 different species:
N. alkalisoli
,
N. galegae
and
N. huautlense
and
Mesorhizobium
(1.75 %) represented by the species
M. camelthorni
. Most of the isolated strains tolerated up to 4 % (w/v) NaCl and grew at 45 °C. This study is the first report on the characterization of
G. saharae
microsymbionts in the Algerian Sahara.</description><identifier>ISSN: 0334-5114</identifier><identifier>EISSN: 1878-7665</identifier><identifier>DOI: 10.1007/s13199-016-0414-y</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biodiversity and Ecology ; Biomedical and Life Sciences ; Desertification ; Developmental Biology ; Drought resistance ; Ecology ; Environmental Sciences ; Evolutionary Biology ; Genetic diversity ; Legumes ; Life Sciences ; Microbiology ; Phylogeny ; Plant Sciences ; Preservation ; RecA protein ; Root nodules ; rRNA 16S ; Sodium chloride ; Species ; Strains (organisms)</subject><ispartof>Symbiosis (Philadelphia, Pa.), 2017-02, Vol.71 (2), p.111-120</ispartof><rights>Springer Science+Business Media Dordrecht 2016</rights><rights>Copyright Springer Science & Business Media 2017</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c350t-482634c396059a90ec81fb6f7de6fa89f589359552ab5cb7b6cc64029d7893793</citedby><cites>FETCH-LOGICAL-c350t-482634c396059a90ec81fb6f7de6fa89f589359552ab5cb7b6cc64029d7893793</cites><orcidid>0000-0002-3424-3882</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13199-016-0414-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13199-016-0414-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://hal.science/hal-01602774$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Chaïch, Khaled</creatorcontrib><creatorcontrib>Bekki, Abdelkader</creatorcontrib><creatorcontrib>Bouras, Noureddine</creatorcontrib><creatorcontrib>Holtz, Michael D.</creatorcontrib><creatorcontrib>Soussou, Souhir</creatorcontrib><creatorcontrib>Mauré, Lucette</creatorcontrib><creatorcontrib>Brunel, Brigitte</creatorcontrib><creatorcontrib>de Lajudie, Philippe</creatorcontrib><creatorcontrib>Cleyet-Marel, Jean-Claude</creatorcontrib><title>Rhizobial diversity associated with the spontaneous legume Genista saharae in the northeastern Algerian Sahara</title><title>Symbiosis (Philadelphia, Pa.)</title><addtitle>Symbiosis</addtitle><description>Genista saharae
is an indigenous shrub legume that spontaneously grows in the northeastern Algerian Sahara. It is known for efficient dune fixation and soil preservation against desertification, due to its drought tolerance and its contribution to sustainable nitrogen resources implemented by biological N
2
-fixation. In this study, the root nodule bacteria of
G. saharae
were investigated using phenotypic and phylogenetic characterization. A total of 57 rhizobial strains were isolated from nodules from several sites in the hyper-arid region of Metlili and Taibet (east Septentrional Sahara). They all nodulate
G. saharae
species but they differed in their symbiotic efficiency and effectiveness. The genetic diversity was assessed by sequencing three housekeeping genes (
atpD
,
recA
and 16S rRNA). The majority of isolates (81 %) belonged to the genus
Ensifer
(previously
Sinorhizobium
), represented mainly by the species
Ensifer meliloti
. The next most abundant genera were
Neorhizobium
(17 %) with 3 different species:
N. alkalisoli
,
N. galegae
and
N. huautlense
and
Mesorhizobium
(1.75 %) represented by the species
M. camelthorni
. Most of the isolated strains tolerated up to 4 % (w/v) NaCl and grew at 45 °C. This study is the first report on the characterization of
G. saharae
microsymbionts in the Algerian Sahara.</description><subject>Biodiversity and Ecology</subject><subject>Biomedical and Life Sciences</subject><subject>Desertification</subject><subject>Developmental Biology</subject><subject>Drought resistance</subject><subject>Ecology</subject><subject>Environmental Sciences</subject><subject>Evolutionary Biology</subject><subject>Genetic diversity</subject><subject>Legumes</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Phylogeny</subject><subject>Plant Sciences</subject><subject>Preservation</subject><subject>RecA protein</subject><subject>Root nodules</subject><subject>rRNA 16S</subject><subject>Sodium chloride</subject><subject>Species</subject><subject>Strains (organisms)</subject><issn>0334-5114</issn><issn>1878-7665</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp1kU9rGzEQxUVpoK6TD5CboKceNpVW_48mtEnAEGiTs5hda70KG8mV5BTn00f2ltJLTgMzv_cY3kPokpIrSoj6limjxjSEyoZwypvDB7SgWulGSSk-ogVhjDeCUv4Jfc75iRBGJZMLFH6O_jV2Hia88S8uZV8OGHKOvYfiNviPLyMuo8N5F0OB4OI-48lt988O37jgcwGcYYQEDvtwIkNMdUAuLgW8mrYueQj41wk6R2cDTNld_J1L9Pjj-8P1bbO-v7m7Xq2bnglSGq5byXjPjCTCgCGu13To5KA2Tg6gzSC0YcII0UIn-k51su8lJ63ZqHpQhi3R19l3hMnukn-GdLARvL1dre1xV4MirVL8hVb2y8zuUvy9d7nYp7hPob5nqdZEadVyXik6U32KOSc3_LOlxB4rsHMFR2d7rMAeqqadNbmyoQbxn_O7ojcvG4oh</recordid><startdate>20170201</startdate><enddate>20170201</enddate><creator>Chaïch, Khaled</creator><creator>Bekki, Abdelkader</creator><creator>Bouras, Noureddine</creator><creator>Holtz, Michael D.</creator><creator>Soussou, Souhir</creator><creator>Mauré, Lucette</creator><creator>Brunel, Brigitte</creator><creator>de Lajudie, Philippe</creator><creator>Cleyet-Marel, Jean-Claude</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><general>Springer Verlag</general><scope>AAYXX</scope><scope>CITATION</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-3424-3882</orcidid></search><sort><creationdate>20170201</creationdate><title>Rhizobial diversity associated with the spontaneous legume Genista saharae in the northeastern Algerian Sahara</title><author>Chaïch, Khaled ; Bekki, Abdelkader ; Bouras, Noureddine ; Holtz, Michael D. ; Soussou, Souhir ; Mauré, Lucette ; Brunel, Brigitte ; de Lajudie, Philippe ; Cleyet-Marel, Jean-Claude</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-482634c396059a90ec81fb6f7de6fa89f589359552ab5cb7b6cc64029d7893793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Biodiversity and Ecology</topic><topic>Biomedical and Life Sciences</topic><topic>Desertification</topic><topic>Developmental Biology</topic><topic>Drought resistance</topic><topic>Ecology</topic><topic>Environmental Sciences</topic><topic>Evolutionary Biology</topic><topic>Genetic diversity</topic><topic>Legumes</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Phylogeny</topic><topic>Plant Sciences</topic><topic>Preservation</topic><topic>RecA protein</topic><topic>Root nodules</topic><topic>rRNA 16S</topic><topic>Sodium chloride</topic><topic>Species</topic><topic>Strains (organisms)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chaïch, Khaled</creatorcontrib><creatorcontrib>Bekki, Abdelkader</creatorcontrib><creatorcontrib>Bouras, Noureddine</creatorcontrib><creatorcontrib>Holtz, Michael D.</creatorcontrib><creatorcontrib>Soussou, Souhir</creatorcontrib><creatorcontrib>Mauré, Lucette</creatorcontrib><creatorcontrib>Brunel, Brigitte</creatorcontrib><creatorcontrib>de Lajudie, Philippe</creatorcontrib><creatorcontrib>Cleyet-Marel, Jean-Claude</creatorcontrib><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Symbiosis (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chaïch, Khaled</au><au>Bekki, Abdelkader</au><au>Bouras, Noureddine</au><au>Holtz, Michael D.</au><au>Soussou, Souhir</au><au>Mauré, Lucette</au><au>Brunel, Brigitte</au><au>de Lajudie, Philippe</au><au>Cleyet-Marel, Jean-Claude</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rhizobial diversity associated with the spontaneous legume Genista saharae in the northeastern Algerian Sahara</atitle><jtitle>Symbiosis (Philadelphia, Pa.)</jtitle><stitle>Symbiosis</stitle><date>2017-02-01</date><risdate>2017</risdate><volume>71</volume><issue>2</issue><spage>111</spage><epage>120</epage><pages>111-120</pages><issn>0334-5114</issn><eissn>1878-7665</eissn><abstract>Genista saharae
is an indigenous shrub legume that spontaneously grows in the northeastern Algerian Sahara. It is known for efficient dune fixation and soil preservation against desertification, due to its drought tolerance and its contribution to sustainable nitrogen resources implemented by biological N
2
-fixation. In this study, the root nodule bacteria of
G. saharae
were investigated using phenotypic and phylogenetic characterization. A total of 57 rhizobial strains were isolated from nodules from several sites in the hyper-arid region of Metlili and Taibet (east Septentrional Sahara). They all nodulate
G. saharae
species but they differed in their symbiotic efficiency and effectiveness. The genetic diversity was assessed by sequencing three housekeeping genes (
atpD
,
recA
and 16S rRNA). The majority of isolates (81 %) belonged to the genus
Ensifer
(previously
Sinorhizobium
), represented mainly by the species
Ensifer meliloti
. The next most abundant genera were
Neorhizobium
(17 %) with 3 different species:
N. alkalisoli
,
N. galegae
and
N. huautlense
and
Mesorhizobium
(1.75 %) represented by the species
M. camelthorni
. Most of the isolated strains tolerated up to 4 % (w/v) NaCl and grew at 45 °C. This study is the first report on the characterization of
G. saharae
microsymbionts in the Algerian Sahara.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s13199-016-0414-y</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-3424-3882</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0334-5114 |
| ispartof | Symbiosis (Philadelphia, Pa.), 2017-02, Vol.71 (2), p.111-120 |
| issn | 0334-5114 1878-7665 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_01602774v1 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Biodiversity and Ecology Biomedical and Life Sciences Desertification Developmental Biology Drought resistance Ecology Environmental Sciences Evolutionary Biology Genetic diversity Legumes Life Sciences Microbiology Phylogeny Plant Sciences Preservation RecA protein Root nodules rRNA 16S Sodium chloride Species Strains (organisms) |
| title | Rhizobial diversity associated with the spontaneous legume Genista saharae in the northeastern Algerian Sahara |
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