Modulation of interleukin-7 receptor expression characterizes differentiation of CD8 T cells specific for HIV, EBV and CMV
To further understand differentiation and homeostasis of CD8 T cells specific for HIV, Epstein-Barr Virus (EBV) and cytomegalovirus (CMV) during HIV infection, we investigated interleukin-7 receptor alpha (IL-7Ralpha) expression on those virus-specific T cells. Microarrays and cytometry analyses wer...
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| Veröffentlicht in: | AIDS (London) 2005-11, Vol.19 (17), p.1981-1986 |
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| creator | BOUTBOUL, Francois PUTHIER, Denis NGUYEN, Catherine AUTRAN, Brigitte APPAY, Victor PELLE, Olivier AIT-MOHAND, Hocine COMBADIERE, Béhazine CARCELAIN, Ghislaine KATLAMA, Christine ROWLAND-JONES, Sarah L DEBRE, Patrice |
| description | To further understand differentiation and homeostasis of CD8 T cells specific for HIV, Epstein-Barr Virus (EBV) and cytomegalovirus (CMV) during HIV infection, we investigated interleukin-7 receptor alpha (IL-7Ralpha) expression on those virus-specific T cells.
Microarrays and cytometry analyses were performed on peripheral blood mononuclear cells (PBMC), total and tetramer-binding virus-specific CD8 T cells from 66 HIV-infected patients.
Microarray analysis revealed reduced levels of IL-7Ralpha and increased levels of perforin with disease progression in total PBMC. This loss of IL-7Ralpha expression was observed on CD8 T cells and was inversely related to perforin expression. The relative expression of both molecules defined three new subsets: IL-7Ralpha(pos)Perforin(neg); IL-7Ralpha(loneg)Perforin(lo); and IL-7Ralpha(loneg)Perforin(hi) corresponding to naive and effector-memory CD8 differentiation, as assessed by CD45RA/CD11a. The IL-7Ralpha expression decreased along the CD8 differentiation pathway defined by CD27 and CD28. In contrast, IL-7Ralpha expression was down-modulated on all the CD8 T cells specific for HIV, EBV and CMV that were almost exclusively IL-7Ralpha(lo/neg)Perforin(lo) and was parallel with the CD27 expression. In addition, this low IL-7Ralpha expression on HIV-specific CD8 T cells was independent of virus load and T-cell activation and remained stable during the first 6 months of antiretroviral therapy despite successful control of HIV replication.
The relative expression of IL-7Ralpha, perforin reveals new aspects of virus-specific CD8 T cell differentiation, independently of T-cell activation and virus load. This opens new perspectives for understanding homeostasis of those cells and immune-based therapeutic strategies. |
| doi_str_mv | 10.1097/01.aids.0000191919.24185.46 |
| format | Article |
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Microarrays and cytometry analyses were performed on peripheral blood mononuclear cells (PBMC), total and tetramer-binding virus-specific CD8 T cells from 66 HIV-infected patients.
Microarray analysis revealed reduced levels of IL-7Ralpha and increased levels of perforin with disease progression in total PBMC. This loss of IL-7Ralpha expression was observed on CD8 T cells and was inversely related to perforin expression. The relative expression of both molecules defined three new subsets: IL-7Ralpha(pos)Perforin(neg); IL-7Ralpha(loneg)Perforin(lo); and IL-7Ralpha(loneg)Perforin(hi) corresponding to naive and effector-memory CD8 differentiation, as assessed by CD45RA/CD11a. The IL-7Ralpha expression decreased along the CD8 differentiation pathway defined by CD27 and CD28. In contrast, IL-7Ralpha expression was down-modulated on all the CD8 T cells specific for HIV, EBV and CMV that were almost exclusively IL-7Ralpha(lo/neg)Perforin(lo) and was parallel with the CD27 expression. In addition, this low IL-7Ralpha expression on HIV-specific CD8 T cells was independent of virus load and T-cell activation and remained stable during the first 6 months of antiretroviral therapy despite successful control of HIV replication.
The relative expression of IL-7Ralpha, perforin reveals new aspects of virus-specific CD8 T cell differentiation, independently of T-cell activation and virus load. This opens new perspectives for understanding homeostasis of those cells and immune-based therapeutic strategies.</description><identifier>ISSN: 0269-9370</identifier><identifier>EISSN: 1473-5571</identifier><identifier>DOI: 10.1097/01.aids.0000191919.24185.46</identifier><identifier>PMID: 16260904</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>AIDS/HIV ; Antiretroviral Therapy, Highly Active - methods ; Bioinformatics ; Biological and medical sciences ; CD28 Antigens - immunology ; CD8-Positive T-Lymphocytes - immunology ; Cell Differentiation - immunology ; Cell differentiation, maturation, development, hematopoiesis ; Cell physiology ; Cells, Cultured ; Computer Science ; Cytomegalovirus ; Cytomegalovirus Infections - genetics ; Cytomegalovirus Infections - immunology ; Epstein-Barr virus ; Epstein-Barr Virus Infections - genetics ; Epstein-Barr Virus Infections - immunology ; Fundamental and applied biological sciences. Psychology ; Gene Expression - genetics ; HIV Infections - genetics ; HIV Infections - immunology ; HIV-1 - genetics ; HIV-1 - immunology ; Human immunodeficiency virus ; Human viral diseases ; Humans ; Immunodeficiencies ; Immunodeficiencies. Immunoglobulinopathies ; Immunopathology ; Infectious diseases ; Leukocytes, Mononuclear - immunology ; Medical sciences ; Membrane Glycoproteins - analysis ; Molecular and cellular biology ; Oligonucleotide Array Sequence Analysis - methods ; Perforin ; Pore Forming Cytotoxic Proteins ; Receptors, Interleukin-7 - immunology ; Transcription, Genetic - genetics ; Tumor Necrosis Factor Receptor Superfamily, Member 7 - genetics ; Tumor Necrosis Factor Receptor Superfamily, Member 7 - immunology ; Viral diseases ; Viral diseases of the lymphoid tissue and the blood. Aids</subject><ispartof>AIDS (London), 2005-11, Vol.19 (17), p.1981-1986</ispartof><rights>2006 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c496t-f0b79ffc74fcbb54e961c8f321d19aa5969b53aa8ab8dc18efec5ad2a5dcd58b3</citedby><cites>FETCH-LOGICAL-c496t-f0b79ffc74fcbb54e961c8f321d19aa5969b53aa8ab8dc18efec5ad2a5dcd58b3</cites><orcidid>0000-0002-7240-5280</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,781,785,886,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17271235$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16260904$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://amu.hal.science/hal-01595823$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>BOUTBOUL, Francois</creatorcontrib><creatorcontrib>PUTHIER, Denis</creatorcontrib><creatorcontrib>NGUYEN, Catherine</creatorcontrib><creatorcontrib>AUTRAN, Brigitte</creatorcontrib><creatorcontrib>APPAY, Victor</creatorcontrib><creatorcontrib>PELLE, Olivier</creatorcontrib><creatorcontrib>AIT-MOHAND, Hocine</creatorcontrib><creatorcontrib>COMBADIERE, Béhazine</creatorcontrib><creatorcontrib>CARCELAIN, Ghislaine</creatorcontrib><creatorcontrib>KATLAMA, Christine</creatorcontrib><creatorcontrib>ROWLAND-JONES, Sarah L</creatorcontrib><creatorcontrib>DEBRE, Patrice</creatorcontrib><title>Modulation of interleukin-7 receptor expression characterizes differentiation of CD8 T cells specific for HIV, EBV and CMV</title><title>AIDS (London)</title><addtitle>AIDS</addtitle><description>To further understand differentiation and homeostasis of CD8 T cells specific for HIV, Epstein-Barr Virus (EBV) and cytomegalovirus (CMV) during HIV infection, we investigated interleukin-7 receptor alpha (IL-7Ralpha) expression on those virus-specific T cells.
Microarrays and cytometry analyses were performed on peripheral blood mononuclear cells (PBMC), total and tetramer-binding virus-specific CD8 T cells from 66 HIV-infected patients.
Microarray analysis revealed reduced levels of IL-7Ralpha and increased levels of perforin with disease progression in total PBMC. This loss of IL-7Ralpha expression was observed on CD8 T cells and was inversely related to perforin expression. The relative expression of both molecules defined three new subsets: IL-7Ralpha(pos)Perforin(neg); IL-7Ralpha(loneg)Perforin(lo); and IL-7Ralpha(loneg)Perforin(hi) corresponding to naive and effector-memory CD8 differentiation, as assessed by CD45RA/CD11a. The IL-7Ralpha expression decreased along the CD8 differentiation pathway defined by CD27 and CD28. In contrast, IL-7Ralpha expression was down-modulated on all the CD8 T cells specific for HIV, EBV and CMV that were almost exclusively IL-7Ralpha(lo/neg)Perforin(lo) and was parallel with the CD27 expression. In addition, this low IL-7Ralpha expression on HIV-specific CD8 T cells was independent of virus load and T-cell activation and remained stable during the first 6 months of antiretroviral therapy despite successful control of HIV replication.
The relative expression of IL-7Ralpha, perforin reveals new aspects of virus-specific CD8 T cell differentiation, independently of T-cell activation and virus load. This opens new perspectives for understanding homeostasis of those cells and immune-based therapeutic strategies.</description><subject>AIDS/HIV</subject><subject>Antiretroviral Therapy, Highly Active - methods</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>CD28 Antigens - immunology</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>Cell Differentiation - immunology</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Computer Science</subject><subject>Cytomegalovirus</subject><subject>Cytomegalovirus Infections - genetics</subject><subject>Cytomegalovirus Infections - immunology</subject><subject>Epstein-Barr virus</subject><subject>Epstein-Barr Virus Infections - genetics</subject><subject>Epstein-Barr Virus Infections - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - genetics</subject><subject>HIV Infections - genetics</subject><subject>HIV Infections - immunology</subject><subject>HIV-1 - genetics</subject><subject>HIV-1 - immunology</subject><subject>Human immunodeficiency virus</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Immunodeficiencies</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunopathology</subject><subject>Infectious diseases</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Medical sciences</subject><subject>Membrane Glycoproteins - analysis</subject><subject>Molecular and cellular biology</subject><subject>Oligonucleotide Array Sequence Analysis - methods</subject><subject>Perforin</subject><subject>Pore Forming Cytotoxic Proteins</subject><subject>Receptors, Interleukin-7 - immunology</subject><subject>Transcription, Genetic - genetics</subject><subject>Tumor Necrosis Factor Receptor Superfamily, Member 7 - genetics</subject><subject>Tumor Necrosis Factor Receptor Superfamily, Member 7 - immunology</subject><subject>Viral diseases</subject><subject>Viral diseases of the lymphoid tissue and the blood. Aids</subject><issn>0269-9370</issn><issn>1473-5571</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdGO1CAUhonRuOPqKxgSo8kmtkKBAvFqHVdnk9l4s84tOaWQRTtthda4-_RSZ7JzKVyQkO_858CH0BtKSkq0_EBoCaFNJcmL6mWXFadKlLx-glaUS1YIIelTtCJVrQvNJDlDL1L6kXlBlHqOzmhd1UQTvkIPN0M7dzCFoceDx6GfXOzc_DP0hcTRWTdOQ8TuzxhdSgtk7yCCzVR4cAm3wXsXXT-Fx4j1Z4VvsXVdl3AanQ0-WOxzyOZ69x5ffdph6Fu8vtm9RM88dMm9Op7n6PuXq9v1pth--3q9vtwWlut6KjxppPbeSu5t0wjudE2t8qyiLdUAQte6EQxAQaNaS5XzzgpoKxCtbYVq2Dm6OOTeQWfGGPYQ780AwWwut2a5I1RooSr2m2b23YEd4_Brdmky-5CWt0DvhjmZWklOGdf_BanknOVPzuDHA2jjkFJ0_nEESsziM7c3i09z8mn--TS8ztWvj23mZu_aU-1RYAbeHgFIFjofobchnThZSVoxwf4Cp2uqsg</recordid><startdate>20051118</startdate><enddate>20051118</enddate><creator>BOUTBOUL, Francois</creator><creator>PUTHIER, Denis</creator><creator>NGUYEN, Catherine</creator><creator>AUTRAN, Brigitte</creator><creator>APPAY, Victor</creator><creator>PELLE, Olivier</creator><creator>AIT-MOHAND, Hocine</creator><creator>COMBADIERE, Béhazine</creator><creator>CARCELAIN, Ghislaine</creator><creator>KATLAMA, Christine</creator><creator>ROWLAND-JONES, Sarah L</creator><creator>DEBRE, Patrice</creator><general>Lippincott Williams & Wilkins</general><general>Wolters Kluwer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-7240-5280</orcidid></search><sort><creationdate>20051118</creationdate><title>Modulation of interleukin-7 receptor expression characterizes differentiation of CD8 T cells specific for HIV, EBV and CMV</title><author>BOUTBOUL, Francois ; PUTHIER, Denis ; NGUYEN, Catherine ; AUTRAN, Brigitte ; APPAY, Victor ; PELLE, Olivier ; AIT-MOHAND, Hocine ; COMBADIERE, Béhazine ; CARCELAIN, Ghislaine ; KATLAMA, Christine ; ROWLAND-JONES, Sarah L ; DEBRE, Patrice</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c496t-f0b79ffc74fcbb54e961c8f321d19aa5969b53aa8ab8dc18efec5ad2a5dcd58b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>AIDS/HIV</topic><topic>Antiretroviral Therapy, Highly Active - methods</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>CD28 Antigens - immunology</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>Cell Differentiation - immunology</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>Computer Science</topic><topic>Cytomegalovirus</topic><topic>Cytomegalovirus Infections - genetics</topic><topic>Cytomegalovirus Infections - immunology</topic><topic>Epstein-Barr virus</topic><topic>Epstein-Barr Virus Infections - genetics</topic><topic>Epstein-Barr Virus Infections - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression - genetics</topic><topic>HIV Infections - genetics</topic><topic>HIV Infections - immunology</topic><topic>HIV-1 - genetics</topic><topic>HIV-1 - immunology</topic><topic>Human immunodeficiency virus</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Immunodeficiencies</topic><topic>Immunodeficiencies. Immunoglobulinopathies</topic><topic>Immunopathology</topic><topic>Infectious diseases</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Medical sciences</topic><topic>Membrane Glycoproteins - analysis</topic><topic>Molecular and cellular biology</topic><topic>Oligonucleotide Array Sequence Analysis - methods</topic><topic>Perforin</topic><topic>Pore Forming Cytotoxic Proteins</topic><topic>Receptors, Interleukin-7 - immunology</topic><topic>Transcription, Genetic - genetics</topic><topic>Tumor Necrosis Factor Receptor Superfamily, Member 7 - genetics</topic><topic>Tumor Necrosis Factor Receptor Superfamily, Member 7 - immunology</topic><topic>Viral diseases</topic><topic>Viral diseases of the lymphoid tissue and the blood. Aids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BOUTBOUL, Francois</creatorcontrib><creatorcontrib>PUTHIER, Denis</creatorcontrib><creatorcontrib>NGUYEN, Catherine</creatorcontrib><creatorcontrib>AUTRAN, Brigitte</creatorcontrib><creatorcontrib>APPAY, Victor</creatorcontrib><creatorcontrib>PELLE, Olivier</creatorcontrib><creatorcontrib>AIT-MOHAND, Hocine</creatorcontrib><creatorcontrib>COMBADIERE, Béhazine</creatorcontrib><creatorcontrib>CARCELAIN, Ghislaine</creatorcontrib><creatorcontrib>KATLAMA, Christine</creatorcontrib><creatorcontrib>ROWLAND-JONES, Sarah L</creatorcontrib><creatorcontrib>DEBRE, Patrice</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>AIDS (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BOUTBOUL, Francois</au><au>PUTHIER, Denis</au><au>NGUYEN, Catherine</au><au>AUTRAN, Brigitte</au><au>APPAY, Victor</au><au>PELLE, Olivier</au><au>AIT-MOHAND, Hocine</au><au>COMBADIERE, Béhazine</au><au>CARCELAIN, Ghislaine</au><au>KATLAMA, Christine</au><au>ROWLAND-JONES, Sarah L</au><au>DEBRE, Patrice</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of interleukin-7 receptor expression characterizes differentiation of CD8 T cells specific for HIV, EBV and CMV</atitle><jtitle>AIDS (London)</jtitle><addtitle>AIDS</addtitle><date>2005-11-18</date><risdate>2005</risdate><volume>19</volume><issue>17</issue><spage>1981</spage><epage>1986</epage><pages>1981-1986</pages><issn>0269-9370</issn><eissn>1473-5571</eissn><abstract>To further understand differentiation and homeostasis of CD8 T cells specific for HIV, Epstein-Barr Virus (EBV) and cytomegalovirus (CMV) during HIV infection, we investigated interleukin-7 receptor alpha (IL-7Ralpha) expression on those virus-specific T cells.
Microarrays and cytometry analyses were performed on peripheral blood mononuclear cells (PBMC), total and tetramer-binding virus-specific CD8 T cells from 66 HIV-infected patients.
Microarray analysis revealed reduced levels of IL-7Ralpha and increased levels of perforin with disease progression in total PBMC. This loss of IL-7Ralpha expression was observed on CD8 T cells and was inversely related to perforin expression. The relative expression of both molecules defined three new subsets: IL-7Ralpha(pos)Perforin(neg); IL-7Ralpha(loneg)Perforin(lo); and IL-7Ralpha(loneg)Perforin(hi) corresponding to naive and effector-memory CD8 differentiation, as assessed by CD45RA/CD11a. The IL-7Ralpha expression decreased along the CD8 differentiation pathway defined by CD27 and CD28. In contrast, IL-7Ralpha expression was down-modulated on all the CD8 T cells specific for HIV, EBV and CMV that were almost exclusively IL-7Ralpha(lo/neg)Perforin(lo) and was parallel with the CD27 expression. In addition, this low IL-7Ralpha expression on HIV-specific CD8 T cells was independent of virus load and T-cell activation and remained stable during the first 6 months of antiretroviral therapy despite successful control of HIV replication.
The relative expression of IL-7Ralpha, perforin reveals new aspects of virus-specific CD8 T cell differentiation, independently of T-cell activation and virus load. This opens new perspectives for understanding homeostasis of those cells and immune-based therapeutic strategies.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>16260904</pmid><doi>10.1097/01.aids.0000191919.24185.46</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-7240-5280</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | AIDS (London), 2005-11, Vol.19 (17), p.1981-1986 |
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| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_01595823v1 |
| source | MEDLINE; Journals@Ovid Complete; EZB-FREE-00999 freely available EZB journals |
| subjects | AIDS/HIV Antiretroviral Therapy, Highly Active - methods Bioinformatics Biological and medical sciences CD28 Antigens - immunology CD8-Positive T-Lymphocytes - immunology Cell Differentiation - immunology Cell differentiation, maturation, development, hematopoiesis Cell physiology Cells, Cultured Computer Science Cytomegalovirus Cytomegalovirus Infections - genetics Cytomegalovirus Infections - immunology Epstein-Barr virus Epstein-Barr Virus Infections - genetics Epstein-Barr Virus Infections - immunology Fundamental and applied biological sciences. Psychology Gene Expression - genetics HIV Infections - genetics HIV Infections - immunology HIV-1 - genetics HIV-1 - immunology Human immunodeficiency virus Human viral diseases Humans Immunodeficiencies Immunodeficiencies. Immunoglobulinopathies Immunopathology Infectious diseases Leukocytes, Mononuclear - immunology Medical sciences Membrane Glycoproteins - analysis Molecular and cellular biology Oligonucleotide Array Sequence Analysis - methods Perforin Pore Forming Cytotoxic Proteins Receptors, Interleukin-7 - immunology Transcription, Genetic - genetics Tumor Necrosis Factor Receptor Superfamily, Member 7 - genetics Tumor Necrosis Factor Receptor Superfamily, Member 7 - immunology Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids |
| title | Modulation of interleukin-7 receptor expression characterizes differentiation of CD8 T cells specific for HIV, EBV and CMV |
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