An improved non-denaturing method for the purification of spiralin, the main membrane lipoprotein of the pathogenic bacteria Spiroplasma melliferum

•An improved procedure for the purification of a bacterial lipoprotein is presented.•The new procedure shortens the purification from 24h to 4h.•Detergent screening revealed that spiralin is mostly insoluble in Sarkosyl and Triton X-100.•Milligram quantities of highly pure spiralin can be obtained.•...

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