Translatome profiling in dormant and nondormant sunflower (Helianthus annuus) seeds highlights post‐transcriptional regulation of germination
Seed dormancy, which blocks germination in apparently favourable conditions, is a key regulatory control point of plant population establishment. As germination requires de novo translation, its regulation by dormancy is likely to be related to the association of individual transcripts to polysomes....
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| Veröffentlicht in: | The New phytologist 2014-12, Vol.204 (4), p.864-872 |
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| creator | Layat, Elodie Leymarie, Juliette El‐Maarouf‐Bouteau, Hayat Caius, José Langlade, Nicolas Bailly, Christophe |
| description | Seed dormancy, which blocks germination in apparently favourable conditions, is a key regulatory control point of plant population establishment. As germination requires de novo translation, its regulation by dormancy is likely to be related to the association of individual transcripts to polysomes. Here, the polysome‐associated mRNAs, that is, the translatome, were fractionated and characterized with microarrays in dormant and nondormant sunflower (Helianthus annuus) embryos during their imbibition at 10°C, a temperature preventing germination of dormant embryos. Profiling of mRNAs in polysomal complexes revealed that the translatome differs between germinating and nongerminating embryos. Association of transcripts with polysomes reached a maximum after 15 h of imbibition; at this time‐point 194 polysome‐associated transcripts were specifically found in nondormant embryos and 47 in dormant embryos only. The proteins corresponding to the polysomal mRNAs in nondormant embryos appeared to be very pertinent for germination and were involved mainly in transport, regulation of transcription or cell wall modifications. This work demonstrates that seed germination results from a timely regulated and selective recruitment of mRNAs to polysomes, thus opening novel fields of investigation for the understanding of this developmental process. |
| doi_str_mv | 10.1111/nph.13002 |
| format | Article |
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As germination requires de novo translation, its regulation by dormancy is likely to be related to the association of individual transcripts to polysomes. Here, the polysome‐associated mRNAs, that is, the translatome, were fractionated and characterized with microarrays in dormant and nondormant sunflower (Helianthus annuus) embryos during their imbibition at 10°C, a temperature preventing germination of dormant embryos. Profiling of mRNAs in polysomal complexes revealed that the translatome differs between germinating and nongerminating embryos. Association of transcripts with polysomes reached a maximum after 15 h of imbibition; at this time‐point 194 polysome‐associated transcripts were specifically found in nondormant embryos and 47 in dormant embryos only. The proteins corresponding to the polysomal mRNAs in nondormant embryos appeared to be very pertinent for germination and were involved mainly in transport, regulation of transcription or cell wall modifications. This work demonstrates that seed germination results from a timely regulated and selective recruitment of mRNAs to polysomes, thus opening novel fields of investigation for the understanding of this developmental process.</description><identifier>ISSN: 0028-646X</identifier><identifier>EISSN: 1469-8137</identifier><identifier>DOI: 10.1111/nph.13002</identifier><identifier>PMID: 25157915</identifier><language>eng</language><publisher>England: Academic Press</publisher><subject>Animal embryos ; Cell walls ; Development Biology ; Dormancy ; Embryos ; Gene expression regulation ; Gene Expression Regulation, Plant ; Gene regulation ; Genes ; Germination ; Germination - physiology ; Helianthus ; Helianthus - genetics ; Helianthus annuus ; Imbibition ; Life Sciences ; Messenger RNA ; microarray technology ; Oligonucleotide Array Sequence Analysis ; Plant Dormancy - genetics ; Plant population ; Plants ; Polyribosomes ; Polyribosomes - genetics ; polysomal mRNA ; Population establishment ; Profiling ; proteins ; Rapid reports ; recruitment ; RNA, Messenger - metabolism ; seed ; seed dormancy ; Seed germination ; Seeds ; Seeds - genetics ; sunflower (Helianthus annuus) ; Sunflowers ; temperature ; Transcription ; translatome</subject><ispartof>The New phytologist, 2014-12, Vol.204 (4), p.864-872</ispartof><rights>2014 New Phytologist Trust</rights><rights>2014 The Authors. New Phytologist © 2014 New Phytologist Trust</rights><rights>2014 The Authors. New Phytologist © 2014 New Phytologist Trust.</rights><rights>Copyright © 2014 New Phytologist Trust</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5822-ab77fcc414b6cf295571c6ef764476d93d7d5003339a75ef5a674f362bb2b8523</citedby><cites>FETCH-LOGICAL-c5822-ab77fcc414b6cf295571c6ef764476d93d7d5003339a75ef5a674f362bb2b8523</cites><orcidid>0000-0001-8692-042X ; 0000-0002-5651-1446</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/newphytologist.204.4.864$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/newphytologist.204.4.864$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,1417,1433,27924,27925,45574,45575,46409,46833,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25157915$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01537951$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Layat, Elodie</creatorcontrib><creatorcontrib>Leymarie, Juliette</creatorcontrib><creatorcontrib>El‐Maarouf‐Bouteau, Hayat</creatorcontrib><creatorcontrib>Caius, José</creatorcontrib><creatorcontrib>Langlade, Nicolas</creatorcontrib><creatorcontrib>Bailly, Christophe</creatorcontrib><title>Translatome profiling in dormant and nondormant sunflower (Helianthus annuus) seeds highlights post‐transcriptional regulation of germination</title><title>The New phytologist</title><addtitle>New Phytol</addtitle><description>Seed dormancy, which blocks germination in apparently favourable conditions, is a key regulatory control point of plant population establishment. As germination requires de novo translation, its regulation by dormancy is likely to be related to the association of individual transcripts to polysomes. Here, the polysome‐associated mRNAs, that is, the translatome, were fractionated and characterized with microarrays in dormant and nondormant sunflower (Helianthus annuus) embryos during their imbibition at 10°C, a temperature preventing germination of dormant embryos. Profiling of mRNAs in polysomal complexes revealed that the translatome differs between germinating and nongerminating embryos. Association of transcripts with polysomes reached a maximum after 15 h of imbibition; at this time‐point 194 polysome‐associated transcripts were specifically found in nondormant embryos and 47 in dormant embryos only. The proteins corresponding to the polysomal mRNAs in nondormant embryos appeared to be very pertinent for germination and were involved mainly in transport, regulation of transcription or cell wall modifications. This work demonstrates that seed germination results from a timely regulated and selective recruitment of mRNAs to polysomes, thus opening novel fields of investigation for the understanding of this developmental process.</description><subject>Animal embryos</subject><subject>Cell walls</subject><subject>Development Biology</subject><subject>Dormancy</subject><subject>Embryos</subject><subject>Gene expression regulation</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene regulation</subject><subject>Genes</subject><subject>Germination</subject><subject>Germination - physiology</subject><subject>Helianthus</subject><subject>Helianthus - genetics</subject><subject>Helianthus annuus</subject><subject>Imbibition</subject><subject>Life Sciences</subject><subject>Messenger RNA</subject><subject>microarray technology</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Plant Dormancy - genetics</subject><subject>Plant population</subject><subject>Plants</subject><subject>Polyribosomes</subject><subject>Polyribosomes - genetics</subject><subject>polysomal mRNA</subject><subject>Population establishment</subject><subject>Profiling</subject><subject>proteins</subject><subject>Rapid reports</subject><subject>recruitment</subject><subject>RNA, Messenger - metabolism</subject><subject>seed</subject><subject>seed dormancy</subject><subject>Seed germination</subject><subject>Seeds</subject><subject>Seeds - genetics</subject><subject>sunflower (Helianthus annuus)</subject><subject>Sunflowers</subject><subject>temperature</subject><subject>Transcription</subject><subject>translatome</subject><issn>0028-646X</issn><issn>1469-8137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kt-K1DAUxoso7rh64QtoYG92L7qbP03SXC6LOsKggrvgXUjbtM2QJjVpHebON9Bn9EnM7OyMIGgghHP45TtfzkmWvUTwEqV15cb-EhEI8aNsgQom8hIR_jhbpEyZs4J9OcmexbiGEArK8NPsBFNEuUB0kf24DcpFqyY_aDAG3xprXAeMA40Pg3ITUK4BzrtDGGfXWr_RAZwvtTUp1c8xQW6e4wWIWjcR9KbrbdpTBKOP06_vP6ddlTqYcTLeKQuC7uZUNAXAt6DTYTDuPnyePWmVjfrFw3ma3b19c3uzzFcf372_uV7lNS0xzlXFeVvXBSoqVrdYUMpRzXTLWVFw1gjS8IZCSAgRilPdUsV40RKGqwpXJcXkNLvY6_bKyjGYQYWt9MrI5fVK7nIQUcIFRd9QYs_3bOrP11nHSQ4m1tpa5bSfo0QMQ1pyLmBCz_5C134O6cVRpp6TsuQEov9RiCFRYCzK4o_FOvgYg26PPhGUu7nLNHd5P_fEvnpQnKtBN0fyMOgEXO2BjbF6-28l-eHT8iCZ72-s4-TD8YbTm7HfTt76ziTjGBaykCXb2X2951vlpeqCifLuM05tTN8OcSEo-Q09_dLB</recordid><startdate>201412</startdate><enddate>201412</enddate><creator>Layat, Elodie</creator><creator>Leymarie, Juliette</creator><creator>El‐Maarouf‐Bouteau, Hayat</creator><creator>Caius, José</creator><creator>Langlade, Nicolas</creator><creator>Bailly, Christophe</creator><general>Academic Press</general><general>New Phytologist Trust</general><general>Wiley Subscription Services, Inc</general><general>Wiley</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-8692-042X</orcidid><orcidid>https://orcid.org/0000-0002-5651-1446</orcidid></search><sort><creationdate>201412</creationdate><title>Translatome profiling in dormant and nondormant sunflower (Helianthus annuus) seeds highlights post‐transcriptional regulation of germination</title><author>Layat, Elodie ; 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As germination requires de novo translation, its regulation by dormancy is likely to be related to the association of individual transcripts to polysomes. Here, the polysome‐associated mRNAs, that is, the translatome, were fractionated and characterized with microarrays in dormant and nondormant sunflower (Helianthus annuus) embryos during their imbibition at 10°C, a temperature preventing germination of dormant embryos. Profiling of mRNAs in polysomal complexes revealed that the translatome differs between germinating and nongerminating embryos. Association of transcripts with polysomes reached a maximum after 15 h of imbibition; at this time‐point 194 polysome‐associated transcripts were specifically found in nondormant embryos and 47 in dormant embryos only. The proteins corresponding to the polysomal mRNAs in nondormant embryos appeared to be very pertinent for germination and were involved mainly in transport, regulation of transcription or cell wall modifications. This work demonstrates that seed germination results from a timely regulated and selective recruitment of mRNAs to polysomes, thus opening novel fields of investigation for the understanding of this developmental process.</abstract><cop>England</cop><pub>Academic Press</pub><pmid>25157915</pmid><doi>10.1111/nph.13002</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-8692-042X</orcidid><orcidid>https://orcid.org/0000-0002-5651-1446</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | The New phytologist, 2014-12, Vol.204 (4), p.864-872 |
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| subjects | Animal embryos Cell walls Development Biology Dormancy Embryos Gene expression regulation Gene Expression Regulation, Plant Gene regulation Genes Germination Germination - physiology Helianthus Helianthus - genetics Helianthus annuus Imbibition Life Sciences Messenger RNA microarray technology Oligonucleotide Array Sequence Analysis Plant Dormancy - genetics Plant population Plants Polyribosomes Polyribosomes - genetics polysomal mRNA Population establishment Profiling proteins Rapid reports recruitment RNA, Messenger - metabolism seed seed dormancy Seed germination Seeds Seeds - genetics sunflower (Helianthus annuus) Sunflowers temperature Transcription translatome |
| title | Translatome profiling in dormant and nondormant sunflower (Helianthus annuus) seeds highlights post‐transcriptional regulation of germination |
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