Exploring medium-chain-length polyhydroxyalkanoates production in the engineered yeast Yarrowia lipolytica
Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) are a large class of biopolymers that have attracted extensive attention as renewable and biodegradable bio-plastics. They are naturally synthesized via fatty acid de novo biosynthesis pathway or β-oxidation pathway from Pseudomonads. The unconven...
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| Veröffentlicht in: | Journal of industrial microbiology & biotechnology 2015-09, Vol.42 (9), p.1255-1262 |
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| creator | Gao, Cuijuan Qi, Qingsheng Madzak, Catherine Lin, Carol Sze Ki |
| description | Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) are a large class of biopolymers that have attracted extensive attention as renewable and biodegradable bio-plastics. They are naturally synthesized via fatty acid de novo biosynthesis pathway or β-oxidation pathway from Pseudomonads. The unconventional yeast Yarrowia lipolytica has excellent lipid/fatty acid catabolism and anabolism capacity depending of the mode of culture. Nevertheless, it cannot naturally synthesize PHA, as it does not express an intrinsic PHA synthase. Here, we constructed a genetically modified strain of Y. lipolytica by heterologously expressing PhaC1 gene from P. aeruginosa PAO1 with a PTS1 peroxisomal signal. When in single copy, the codon optimized PhaC1 allowed the synthesis of 0.205 % DCW of PHA after 72 h cultivation in YNBD medium containing 0.1 % oleic acid. By using a multi-copy integration strategy, PHA content increased to 2.84 % DCW when the concentration of oleic acid in YNBD was 1.0 %. Furthermore, when the recombinant yeast was grown in the medium containing triolein, PHA accumulated up to 5.0 % DCW with as high as 21.9 g/L DCW, which represented 1.11 g/L in the culture. Our results demonstrated the potential use of Y. lipolytica as a promising microbial cell factory for PHA production using food waste, which contains lipids and other essential nutrients. |
| doi_str_mv | 10.1007/s10295-015-1649-y |
| format | Article |
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They are naturally synthesized via fatty acid de novo biosynthesis pathway or β-oxidation pathway from Pseudomonads. The unconventional yeast Yarrowia lipolytica has excellent lipid/fatty acid catabolism and anabolism capacity depending of the mode of culture. Nevertheless, it cannot naturally synthesize PHA, as it does not express an intrinsic PHA synthase. Here, we constructed a genetically modified strain of Y. lipolytica by heterologously expressing PhaC1 gene from P. aeruginosa PAO1 with a PTS1 peroxisomal signal. When in single copy, the codon optimized PhaC1 allowed the synthesis of 0.205 % DCW of PHA after 72 h cultivation in YNBD medium containing 0.1 % oleic acid. By using a multi-copy integration strategy, PHA content increased to 2.84 % DCW when the concentration of oleic acid in YNBD was 1.0 %. Furthermore, when the recombinant yeast was grown in the medium containing triolein, PHA accumulated up to 5.0 % DCW with as high as 21.9 g/L DCW, which represented 1.11 g/L in the culture. Our results demonstrated the potential use of Y. lipolytica as a promising microbial cell factory for PHA production using food waste, which contains lipids and other essential nutrients.</description><identifier>ISSN: 1367-5435</identifier><identifier>EISSN: 1476-5535</identifier><identifier>DOI: 10.1007/s10295-015-1649-y</identifier><identifier>PMID: 26153503</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acyltransferases - biosynthesis ; Acyltransferases - genetics ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - genetics ; beta oxidation ; Biochemistry ; biodegradability ; Biodegradation ; Bioinformatics ; Biomedical and Life Sciences ; bioplastics ; Biopolymers ; Biosynthesis ; Biotechnology ; Biotechnology industry ; Carbon ; E coli ; Enzymes ; Essential nutrients ; Fatty acids ; Fermentation ; Food waste ; Genes ; Genetic Engineering ; genetically engineered microorganisms ; Inorganic Chemistry ; Life Sciences ; Lipids ; Metabolic Engineering and Synthetic Biology ; Metabolism ; Metabolites ; Microbiology ; nutrients ; oleic acid ; Oleic Acid - metabolism ; Oxidation ; Plasmids ; Polyhydroxyalkanoates ; Polyhydroxyalkanoates - biosynthesis ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - enzymology ; Studies ; triolein ; Triolein - metabolism ; Yarrowia - genetics ; Yarrowia - metabolism ; Yarrowia lipolytica ; Yeast ; Yeasts</subject><ispartof>Journal of industrial microbiology & biotechnology, 2015-09, Vol.42 (9), p.1255-1262</ispartof><rights>Society for Industrial Microbiology 2015 2015</rights><rights>Society for Industrial Microbiology and Biotechnology 2015</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c607t-8afed1d6a6ddfe912d1c347515886d3ecf5a653aa7d83d7a3a31206b133b4e0b3</citedby><cites>FETCH-LOGICAL-c607t-8afed1d6a6ddfe912d1c347515886d3ecf5a653aa7d83d7a3a31206b133b4e0b3</cites><orcidid>0000-0002-1413-0602 ; 0000-0002-8493-4307</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10295-015-1649-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10295-015-1649-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26153503$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01535288$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Cuijuan</creatorcontrib><creatorcontrib>Qi, Qingsheng</creatorcontrib><creatorcontrib>Madzak, Catherine</creatorcontrib><creatorcontrib>Lin, Carol Sze Ki</creatorcontrib><title>Exploring medium-chain-length polyhydroxyalkanoates production in the engineered yeast Yarrowia lipolytica</title><title>Journal of industrial microbiology & biotechnology</title><addtitle>J Ind Microbiol Biotechnol</addtitle><addtitle>J Ind Microbiol Biotechnol</addtitle><description>Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) are a large class of biopolymers that have attracted extensive attention as renewable and biodegradable bio-plastics. They are naturally synthesized via fatty acid de novo biosynthesis pathway or β-oxidation pathway from Pseudomonads. The unconventional yeast Yarrowia lipolytica has excellent lipid/fatty acid catabolism and anabolism capacity depending of the mode of culture. Nevertheless, it cannot naturally synthesize PHA, as it does not express an intrinsic PHA synthase. Here, we constructed a genetically modified strain of Y. lipolytica by heterologously expressing PhaC1 gene from P. aeruginosa PAO1 with a PTS1 peroxisomal signal. When in single copy, the codon optimized PhaC1 allowed the synthesis of 0.205 % DCW of PHA after 72 h cultivation in YNBD medium containing 0.1 % oleic acid. By using a multi-copy integration strategy, PHA content increased to 2.84 % DCW when the concentration of oleic acid in YNBD was 1.0 %. Furthermore, when the recombinant yeast was grown in the medium containing triolein, PHA accumulated up to 5.0 % DCW with as high as 21.9 g/L DCW, which represented 1.11 g/L in the culture. Our results demonstrated the potential use of Y. lipolytica as a promising microbial cell factory for PHA production using food waste, which contains lipids and other essential nutrients.</description><subject>Acyltransferases - biosynthesis</subject><subject>Acyltransferases - genetics</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - genetics</subject><subject>beta oxidation</subject><subject>Biochemistry</subject><subject>biodegradability</subject><subject>Biodegradation</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>bioplastics</subject><subject>Biopolymers</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Biotechnology industry</subject><subject>Carbon</subject><subject>E coli</subject><subject>Enzymes</subject><subject>Essential nutrients</subject><subject>Fatty acids</subject><subject>Fermentation</subject><subject>Food waste</subject><subject>Genes</subject><subject>Genetic Engineering</subject><subject>genetically engineered microorganisms</subject><subject>Inorganic Chemistry</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Metabolic Engineering and Synthetic Biology</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Microbiology</subject><subject>nutrients</subject><subject>oleic acid</subject><subject>Oleic Acid - 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Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of industrial microbiology & biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gao, Cuijuan</au><au>Qi, Qingsheng</au><au>Madzak, Catherine</au><au>Lin, Carol Sze Ki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exploring medium-chain-length polyhydroxyalkanoates production in the engineered yeast Yarrowia lipolytica</atitle><jtitle>Journal of industrial microbiology & biotechnology</jtitle><stitle>J Ind Microbiol Biotechnol</stitle><addtitle>J Ind Microbiol Biotechnol</addtitle><date>2015-09-01</date><risdate>2015</risdate><volume>42</volume><issue>9</issue><spage>1255</spage><epage>1262</epage><pages>1255-1262</pages><issn>1367-5435</issn><eissn>1476-5535</eissn><abstract>Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) are a large class of biopolymers that have attracted extensive attention as renewable and biodegradable bio-plastics. They are naturally synthesized via fatty acid de novo biosynthesis pathway or β-oxidation pathway from Pseudomonads. The unconventional yeast Yarrowia lipolytica has excellent lipid/fatty acid catabolism and anabolism capacity depending of the mode of culture. Nevertheless, it cannot naturally synthesize PHA, as it does not express an intrinsic PHA synthase. Here, we constructed a genetically modified strain of Y. lipolytica by heterologously expressing PhaC1 gene from P. aeruginosa PAO1 with a PTS1 peroxisomal signal. When in single copy, the codon optimized PhaC1 allowed the synthesis of 0.205 % DCW of PHA after 72 h cultivation in YNBD medium containing 0.1 % oleic acid. By using a multi-copy integration strategy, PHA content increased to 2.84 % DCW when the concentration of oleic acid in YNBD was 1.0 %. Furthermore, when the recombinant yeast was grown in the medium containing triolein, PHA accumulated up to 5.0 % DCW with as high as 21.9 g/L DCW, which represented 1.11 g/L in the culture. Our results demonstrated the potential use of Y. lipolytica as a promising microbial cell factory for PHA production using food waste, which contains lipids and other essential nutrients.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26153503</pmid><doi>10.1007/s10295-015-1649-y</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-1413-0602</orcidid><orcidid>https://orcid.org/0000-0002-8493-4307</orcidid></addata></record> |
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| ispartof | Journal of industrial microbiology & biotechnology, 2015-09, Vol.42 (9), p.1255-1262 |
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| subjects | Acyltransferases - biosynthesis Acyltransferases - genetics Bacterial Proteins - biosynthesis Bacterial Proteins - genetics beta oxidation Biochemistry biodegradability Biodegradation Bioinformatics Biomedical and Life Sciences bioplastics Biopolymers Biosynthesis Biotechnology Biotechnology industry Carbon E coli Enzymes Essential nutrients Fatty acids Fermentation Food waste Genes Genetic Engineering genetically engineered microorganisms Inorganic Chemistry Life Sciences Lipids Metabolic Engineering and Synthetic Biology Metabolism Metabolites Microbiology nutrients oleic acid Oleic Acid - metabolism Oxidation Plasmids Polyhydroxyalkanoates Polyhydroxyalkanoates - biosynthesis Pseudomonas aeruginosa Pseudomonas aeruginosa - enzymology Studies triolein Triolein - metabolism Yarrowia - genetics Yarrowia - metabolism Yarrowia lipolytica Yeast Yeasts |
| title | Exploring medium-chain-length polyhydroxyalkanoates production in the engineered yeast Yarrowia lipolytica |
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