Response of the pearl oyster Pinctada margaritifera to cadmium and chromium: Identification of molecular biomarkers
This study was designed to identify in the pearl oyster Pinctada margaritifera, used as a bio-accumulator, molecular biomarkers for the presence of heavy metals in the lagoon environment. Pearl oysters were exposed to 2 concentrations (1 and 10μgL−1) of cadmium (Cd) and chromium (Cr) compared to a c...
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| Veröffentlicht in: | Marine pollution bulletin 2017-05, Vol.118 (1-2), p.420-426 |
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| container_title | Marine pollution bulletin |
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| creator | Gueguen, Yannick Denis, Saulnier Adrien, Santini Kevin, Magre Pierre, Garen Solène, Bernagout Marine, Nohl Patrick, Bouisset Herehia, Helme Serge, Planes Gilles, Le Moullac |
| description | This study was designed to identify in the pearl oyster Pinctada margaritifera, used as a bio-accumulator, molecular biomarkers for the presence of heavy metals in the lagoon environment. Pearl oysters were exposed to 2 concentrations (1 and 10μgL−1) of cadmium (Cd) and chromium (Cr) compared to a control. Twelve target genes encoding proteins potentially involved in the response to heavy metal contamination with antioxidant, detoxification or apoptosis activities were selected. P. margaritifera accumulated Cd but not Cr, and mortality was related to the amount of Cd accumulated in tissues. In response to Cd-Cr contamination, metallothionein (MT) was significantly up-regulated by Cd-Cr at both concentrations, while 7 others (SOD, CAT, GPX, GSTO, GSTM, CASP, MDR) were down-regulated. Based on the development of these molecular tools, we propose that the pearl oyster, P. margaritifera, could be used as a sentinel species for heavy metal contamination in the lagoons of tropical ecosystems.
•The pearl oyster Pinctada margaritifera accumulated cadmium.•Metallothionein gene expression was significantly up-regulated by cadmium.•P. margaritifera could be a sentinel species of the heavy metals contamination. |
| doi_str_mv | 10.1016/j.marpolbul.2017.03.012 |
| format | Article |
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•The pearl oyster Pinctada margaritifera accumulated cadmium.•Metallothionein gene expression was significantly up-regulated by cadmium.•P. margaritifera could be a sentinel species of the heavy metals contamination.</description><identifier>ISSN: 0025-326X</identifier><identifier>EISSN: 1879-3363</identifier><identifier>DOI: 10.1016/j.marpolbul.2017.03.012</identifier><identifier>PMID: 28320509</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animal biology ; Animals ; Biomarkers - analysis ; Cadmium ; Cadmium - toxicity ; Chromium ; Chromium - toxicity ; Ecosystem ; Ecotoxicology ; French Polynesia ; Gene expression ; Invertebrate Zoology ; Life Sciences ; Metallothionein ; Pinctada - drug effects ; Pinctada - genetics ; Pinctada margaritifera ; Toxicology</subject><ispartof>Marine pollution bulletin, 2017-05, Vol.118 (1-2), p.420-426</ispartof><rights>2017 Elsevier Ltd</rights><rights>Copyright © 2017 Elsevier Ltd. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-d4392921ea8f17ac7c30bbce6181db1de62d081daee3f539b0c7451c3cc1af263</citedby><cites>FETCH-LOGICAL-c454t-d4392921ea8f17ac7c30bbce6181db1de62d081daee3f539b0c7451c3cc1af263</cites><orcidid>0000-0002-8749-9582 ; 0000-0002-5689-5371</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.marpolbul.2017.03.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28320509$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01526382$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Gueguen, Yannick</creatorcontrib><creatorcontrib>Denis, Saulnier</creatorcontrib><creatorcontrib>Adrien, Santini</creatorcontrib><creatorcontrib>Kevin, Magre</creatorcontrib><creatorcontrib>Pierre, Garen</creatorcontrib><creatorcontrib>Solène, Bernagout</creatorcontrib><creatorcontrib>Marine, Nohl</creatorcontrib><creatorcontrib>Patrick, Bouisset</creatorcontrib><creatorcontrib>Herehia, Helme</creatorcontrib><creatorcontrib>Serge, Planes</creatorcontrib><creatorcontrib>Gilles, Le Moullac</creatorcontrib><title>Response of the pearl oyster Pinctada margaritifera to cadmium and chromium: Identification of molecular biomarkers</title><title>Marine pollution bulletin</title><addtitle>Mar Pollut Bull</addtitle><description>This study was designed to identify in the pearl oyster Pinctada margaritifera, used as a bio-accumulator, molecular biomarkers for the presence of heavy metals in the lagoon environment. Pearl oysters were exposed to 2 concentrations (1 and 10μgL−1) of cadmium (Cd) and chromium (Cr) compared to a control. Twelve target genes encoding proteins potentially involved in the response to heavy metal contamination with antioxidant, detoxification or apoptosis activities were selected. P. margaritifera accumulated Cd but not Cr, and mortality was related to the amount of Cd accumulated in tissues. In response to Cd-Cr contamination, metallothionein (MT) was significantly up-regulated by Cd-Cr at both concentrations, while 7 others (SOD, CAT, GPX, GSTO, GSTM, CASP, MDR) were down-regulated. Based on the development of these molecular tools, we propose that the pearl oyster, P. margaritifera, could be used as a sentinel species for heavy metal contamination in the lagoons of tropical ecosystems.
•The pearl oyster Pinctada margaritifera accumulated cadmium.•Metallothionein gene expression was significantly up-regulated by cadmium.•P. margaritifera could be a sentinel species of the heavy metals contamination.</description><subject>Animal biology</subject><subject>Animals</subject><subject>Biomarkers - analysis</subject><subject>Cadmium</subject><subject>Cadmium - toxicity</subject><subject>Chromium</subject><subject>Chromium - toxicity</subject><subject>Ecosystem</subject><subject>Ecotoxicology</subject><subject>French Polynesia</subject><subject>Gene expression</subject><subject>Invertebrate Zoology</subject><subject>Life Sciences</subject><subject>Metallothionein</subject><subject>Pinctada - drug effects</subject><subject>Pinctada - genetics</subject><subject>Pinctada margaritifera</subject><subject>Toxicology</subject><issn>0025-326X</issn><issn>1879-3363</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAURi0EokPpXwAvYZHgxzgPdqOKPqSRQAik7qwb-4bxkMTBdir13-NoymxZ-aFzvyPdj5D3nJWc8erTsRwhzH7olqEUjNclkyXj4gXZ8KZuCykr-ZJsGBOqkKJ6uCBvYjwyxmpR89fkQjRSMMXaDYnfMc5-ikh9T9MB6YwQBuqfYsJAv7nJJLBAs-0XBJdcjwFo8tSAHd0yUpgsNYfg18dnem9xyowzkJyf1sjRD2iWAQLtnM8pvzHEt-RVD0PEq-fzkvy8-fLj-q7Yf729v97tC7NV21TYrWxFKzhC0_MaTG0k6zqDFW-47bjFSliWr4AoeyXbjpl6q7iRxnDoRSUvycdT7gEGPQeX9U_ag9N3u71e_xhXGWvEI8_shxM7B_9nwZj06KLBYYAJ_RL1utZKNY1SGa1PqAk-xoD9OZszvbajj_rcjl7b0Uxml8iT754lSzeiPc_9qyMDuxOAeS2PDoOOxuFk0LqAJmnr3X8lfwGbdacX</recordid><startdate>20170515</startdate><enddate>20170515</enddate><creator>Gueguen, Yannick</creator><creator>Denis, Saulnier</creator><creator>Adrien, Santini</creator><creator>Kevin, Magre</creator><creator>Pierre, Garen</creator><creator>Solène, Bernagout</creator><creator>Marine, Nohl</creator><creator>Patrick, Bouisset</creator><creator>Herehia, Helme</creator><creator>Serge, Planes</creator><creator>Gilles, Le Moullac</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0002-8749-9582</orcidid><orcidid>https://orcid.org/0000-0002-5689-5371</orcidid></search><sort><creationdate>20170515</creationdate><title>Response of the pearl oyster Pinctada margaritifera to cadmium and chromium: Identification of molecular biomarkers</title><author>Gueguen, Yannick ; Denis, Saulnier ; Adrien, Santini ; Kevin, Magre ; Pierre, Garen ; Solène, Bernagout ; Marine, Nohl ; Patrick, Bouisset ; Herehia, Helme ; Serge, Planes ; Gilles, Le Moullac</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-d4392921ea8f17ac7c30bbce6181db1de62d081daee3f539b0c7451c3cc1af263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animal biology</topic><topic>Animals</topic><topic>Biomarkers - analysis</topic><topic>Cadmium</topic><topic>Cadmium - toxicity</topic><topic>Chromium</topic><topic>Chromium - toxicity</topic><topic>Ecosystem</topic><topic>Ecotoxicology</topic><topic>French Polynesia</topic><topic>Gene expression</topic><topic>Invertebrate Zoology</topic><topic>Life Sciences</topic><topic>Metallothionein</topic><topic>Pinctada - drug effects</topic><topic>Pinctada - genetics</topic><topic>Pinctada margaritifera</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gueguen, Yannick</creatorcontrib><creatorcontrib>Denis, Saulnier</creatorcontrib><creatorcontrib>Adrien, Santini</creatorcontrib><creatorcontrib>Kevin, Magre</creatorcontrib><creatorcontrib>Pierre, Garen</creatorcontrib><creatorcontrib>Solène, Bernagout</creatorcontrib><creatorcontrib>Marine, Nohl</creatorcontrib><creatorcontrib>Patrick, Bouisset</creatorcontrib><creatorcontrib>Herehia, Helme</creatorcontrib><creatorcontrib>Serge, Planes</creatorcontrib><creatorcontrib>Gilles, Le Moullac</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Marine pollution bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gueguen, Yannick</au><au>Denis, Saulnier</au><au>Adrien, Santini</au><au>Kevin, Magre</au><au>Pierre, Garen</au><au>Solène, Bernagout</au><au>Marine, Nohl</au><au>Patrick, Bouisset</au><au>Herehia, Helme</au><au>Serge, Planes</au><au>Gilles, Le Moullac</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Response of the pearl oyster Pinctada margaritifera to cadmium and chromium: Identification of molecular biomarkers</atitle><jtitle>Marine pollution bulletin</jtitle><addtitle>Mar Pollut Bull</addtitle><date>2017-05-15</date><risdate>2017</risdate><volume>118</volume><issue>1-2</issue><spage>420</spage><epage>426</epage><pages>420-426</pages><issn>0025-326X</issn><eissn>1879-3363</eissn><abstract>This study was designed to identify in the pearl oyster Pinctada margaritifera, used as a bio-accumulator, molecular biomarkers for the presence of heavy metals in the lagoon environment. Pearl oysters were exposed to 2 concentrations (1 and 10μgL−1) of cadmium (Cd) and chromium (Cr) compared to a control. Twelve target genes encoding proteins potentially involved in the response to heavy metal contamination with antioxidant, detoxification or apoptosis activities were selected. P. margaritifera accumulated Cd but not Cr, and mortality was related to the amount of Cd accumulated in tissues. In response to Cd-Cr contamination, metallothionein (MT) was significantly up-regulated by Cd-Cr at both concentrations, while 7 others (SOD, CAT, GPX, GSTO, GSTM, CASP, MDR) were down-regulated. Based on the development of these molecular tools, we propose that the pearl oyster, P. margaritifera, could be used as a sentinel species for heavy metal contamination in the lagoons of tropical ecosystems.
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| fulltext | fulltext |
| identifier | ISSN: 0025-326X |
| ispartof | Marine pollution bulletin, 2017-05, Vol.118 (1-2), p.420-426 |
| issn | 0025-326X 1879-3363 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_01526382v1 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animal biology Animals Biomarkers - analysis Cadmium Cadmium - toxicity Chromium Chromium - toxicity Ecosystem Ecotoxicology French Polynesia Gene expression Invertebrate Zoology Life Sciences Metallothionein Pinctada - drug effects Pinctada - genetics Pinctada margaritifera Toxicology |
| title | Response of the pearl oyster Pinctada margaritifera to cadmium and chromium: Identification of molecular biomarkers |
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