Integrated electrochemical biosensor based on algal metabolism for water toxicity analysis
An autonomous electrochemical biosensor with three electrodes integrated on the same silicon chip dedicated to the detection of herbicides in water was fabricated by means of silicon-based microfabrication technology. Platinum (Pt), platinum black (Pt Bl), tungsten/tungsten oxide (W/WO3) and iridium...
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| Veröffentlicht in: | Biosensors & bioelectronics 2014-11, Vol.61, p.290-297 |
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| creator | Tsopela, A. Lale, A. Vanhove, E. Reynes, O. Séguy, I. Temple-Boyer, P. Juneau, P. Izquierdo, R. Launay, J. |
| description | An autonomous electrochemical biosensor with three electrodes integrated on the same silicon chip dedicated to the detection of herbicides in water was fabricated by means of silicon-based microfabrication technology. Platinum (Pt), platinum black (Pt Bl), tungsten/tungsten oxide (W/WO3) and iridium oxide (Pt/IrO2) working ultramicroelectrodes were developed. Ag/AgCl and Pt electrodes were used as reference and counter-integrated electrodes respectively. Physical vapor deposition (PVD) and electrodeposition were used for thin film deposition. The ultramicroelectrodes were employed for the detection of O2, H2O2 and pH related ions H3O+/OH−, species taking part in photosynthetic and metabolic activities of algae. By measuring the variations in consumption–production rates of these electroactive species by algae, the quantity of herbicides present at trace level in the solution can be estimated. Fabricated ultramicroelectrodes were electrochemically characterized and calibrated. Pt Black ultramicroelectrodes exhibited the greatest sensitivity regarding O2 and H2O2 detection while Pt/IrO2 ultramicroelectrodes were more sensitive for pH measurement compared to W/WO3 ultramicroelectrodes for pH measurement. Bioassays were then conducted to detect traces of Diuron herbicide in water samples by evaluating disturbances in photosynthetic and metabolic activities of algae caused by this herbicide.
•This paper presents the development of an electrochemical micro-biosensor for the detection of environmental pollution.•Integrated electrochemical microcells were fabricated.•The electrodes were calibrated and used in bioassays in algal solutions.•O2 evolution was measured in algal solutions.•LOD of 0.2µM for the detection of Diuron herbicide is reported. |
| doi_str_mv | 10.1016/j.bios.2014.05.004 |
| format | Article |
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•This paper presents the development of an electrochemical micro-biosensor for the detection of environmental pollution.•Integrated electrochemical microcells were fabricated.•The electrodes were calibrated and used in bioassays in algal solutions.•O2 evolution was measured in algal solutions.•LOD of 0.2µM for the detection of Diuron herbicide is reported.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2014.05.004</identifier><identifier>PMID: 24906088</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Algae ; Algal metabolism ; Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biosensors ; Biotechnology ; Chlamydomonas reinhardtii - metabolism ; Diuron - analysis ; Diuron - metabolism ; Electrochemical Techniques - instrumentation ; Electrodes ; Engineering Sciences ; Equipment Design ; Fundamental and applied biological sciences. Psychology ; Herbicides ; Herbicides - analysis ; Herbicides - metabolism ; Hydrogen peroxide ; Hydrogen Peroxide - analysis ; Hydrogen Peroxide - metabolism ; Limit of Detection ; Methods. Procedures. Technologies ; Micro and nanotechnologies ; Microelectrodes ; Microelectronics ; Oxygen ; Oxygen - analysis ; Oxygen - metabolism ; Pesticides detection ; pH measurement ; Physical vapor deposition ; Platinum ; Tungsten oxides ; Ultramicroelectrodes ; Various methods and equipments ; Water Pollutants, Chemical - analysis ; Water Pollutants, Chemical - metabolism</subject><ispartof>Biosensors & bioelectronics, 2014-11, Vol.61, p.290-297</ispartof><rights>2014 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c567t-b22f680e595ec5545ec3723cb2935977d473d23384184dcd87db6eebc3be8cde3</citedby><cites>FETCH-LOGICAL-c567t-b22f680e595ec5545ec3723cb2935977d473d23384184dcd87db6eebc3be8cde3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2014.05.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28595969$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24906088$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01504978$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsopela, A.</creatorcontrib><creatorcontrib>Lale, A.</creatorcontrib><creatorcontrib>Vanhove, E.</creatorcontrib><creatorcontrib>Reynes, O.</creatorcontrib><creatorcontrib>Séguy, I.</creatorcontrib><creatorcontrib>Temple-Boyer, P.</creatorcontrib><creatorcontrib>Juneau, P.</creatorcontrib><creatorcontrib>Izquierdo, R.</creatorcontrib><creatorcontrib>Launay, J.</creatorcontrib><title>Integrated electrochemical biosensor based on algal metabolism for water toxicity analysis</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>An autonomous electrochemical biosensor with three electrodes integrated on the same silicon chip dedicated to the detection of herbicides in water was fabricated by means of silicon-based microfabrication technology. Platinum (Pt), platinum black (Pt Bl), tungsten/tungsten oxide (W/WO3) and iridium oxide (Pt/IrO2) working ultramicroelectrodes were developed. Ag/AgCl and Pt electrodes were used as reference and counter-integrated electrodes respectively. Physical vapor deposition (PVD) and electrodeposition were used for thin film deposition. The ultramicroelectrodes were employed for the detection of O2, H2O2 and pH related ions H3O+/OH−, species taking part in photosynthetic and metabolic activities of algae. By measuring the variations in consumption–production rates of these electroactive species by algae, the quantity of herbicides present at trace level in the solution can be estimated. Fabricated ultramicroelectrodes were electrochemically characterized and calibrated. Pt Black ultramicroelectrodes exhibited the greatest sensitivity regarding O2 and H2O2 detection while Pt/IrO2 ultramicroelectrodes were more sensitive for pH measurement compared to W/WO3 ultramicroelectrodes for pH measurement. Bioassays were then conducted to detect traces of Diuron herbicide in water samples by evaluating disturbances in photosynthetic and metabolic activities of algae caused by this herbicide.
•This paper presents the development of an electrochemical micro-biosensor for the detection of environmental pollution.•Integrated electrochemical microcells were fabricated.•The electrodes were calibrated and used in bioassays in algal solutions.•O2 evolution was measured in algal solutions.•LOD of 0.2µM for the detection of Diuron herbicide is reported.</description><subject>Algae</subject><subject>Algal metabolism</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chlamydomonas reinhardtii - metabolism</subject><subject>Diuron - analysis</subject><subject>Diuron - metabolism</subject><subject>Electrochemical Techniques - instrumentation</subject><subject>Electrodes</subject><subject>Engineering Sciences</subject><subject>Equipment Design</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Herbicides</subject><subject>Herbicides - analysis</subject><subject>Herbicides - metabolism</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - analysis</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Limit of Detection</subject><subject>Methods. Procedures. Technologies</subject><subject>Micro and nanotechnologies</subject><subject>Microelectrodes</subject><subject>Microelectronics</subject><subject>Oxygen</subject><subject>Oxygen - analysis</subject><subject>Oxygen - metabolism</subject><subject>Pesticides detection</subject><subject>pH measurement</subject><subject>Physical vapor deposition</subject><subject>Platinum</subject><subject>Tungsten oxides</subject><subject>Ultramicroelectrodes</subject><subject>Various methods and equipments</subject><subject>Water Pollutants, Chemical - analysis</subject><subject>Water Pollutants, Chemical - metabolism</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1rFDEYxoModrv6D3iQuQj2MGu-P8BLKWoLC1704iVkknfaLDOTmsy27n9vhl3rTYSQQJ7f8_LyPAi9IXhDMJEfdpsuprKhmPANFhuM-TO0IlqxllMmnqMVNkK2Qkp2hs5L2WGMFTH4JTqj3GCJtV6hHzfTDLfZzRAaGMDPOfk7GKN3Q7NMh6mk3HSuVD1NjRtuqzDC7Lo0xDI2fVUfqzs3c_oVfZwPjZvccCixvEIvejcUeH161-j750_frq7b7dcvN1eX29YLqea2o7SXGoMwArwQvN5MUeY7apgwSgWuWKCMaU40Dz5oFToJ0HnWgfYB2BpdHOfeucHe5zi6fLDJRXt9ubXLHyYCc6P0A6ns-yN7n9PPPZTZjrF4GAY3QdoXSySnlBMu6X-gVBmhWT1rRI-oz6mUDP3TGgTbpSq7s0uYdqnKYmFrVdX09jR_340Qnix_uqnAuxPgSq2jz27ysfzldE3MSFO5j0cOasgPEbItPsLkIcRcC7UhxX_t8RtblLHT</recordid><startdate>20141115</startdate><enddate>20141115</enddate><creator>Tsopela, A.</creator><creator>Lale, A.</creator><creator>Vanhove, E.</creator><creator>Reynes, O.</creator><creator>Séguy, I.</creator><creator>Temple-Boyer, P.</creator><creator>Juneau, P.</creator><creator>Izquierdo, R.</creator><creator>Launay, J.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7SP</scope><scope>7U5</scope><scope>L7M</scope><scope>1XC</scope><scope>VOOES</scope></search><sort><creationdate>20141115</creationdate><title>Integrated electrochemical biosensor based on algal metabolism for water toxicity analysis</title><author>Tsopela, A. ; Lale, A. ; Vanhove, E. ; Reynes, O. ; Séguy, I. ; Temple-Boyer, P. ; Juneau, P. ; Izquierdo, R. ; Launay, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c567t-b22f680e595ec5545ec3723cb2935977d473d23384184dcd87db6eebc3be8cde3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Algae</topic><topic>Algal metabolism</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Chlamydomonas reinhardtii - metabolism</topic><topic>Diuron - analysis</topic><topic>Diuron - metabolism</topic><topic>Electrochemical Techniques - instrumentation</topic><topic>Electrodes</topic><topic>Engineering Sciences</topic><topic>Equipment Design</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Herbicides</topic><topic>Herbicides - analysis</topic><topic>Herbicides - metabolism</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - analysis</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Limit of Detection</topic><topic>Methods. Procedures. Technologies</topic><topic>Micro and nanotechnologies</topic><topic>Microelectrodes</topic><topic>Microelectronics</topic><topic>Oxygen</topic><topic>Oxygen - analysis</topic><topic>Oxygen - metabolism</topic><topic>Pesticides detection</topic><topic>pH measurement</topic><topic>Physical vapor deposition</topic><topic>Platinum</topic><topic>Tungsten oxides</topic><topic>Ultramicroelectrodes</topic><topic>Various methods and equipments</topic><topic>Water Pollutants, Chemical - analysis</topic><topic>Water Pollutants, Chemical - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsopela, A.</creatorcontrib><creatorcontrib>Lale, A.</creatorcontrib><creatorcontrib>Vanhove, E.</creatorcontrib><creatorcontrib>Reynes, O.</creatorcontrib><creatorcontrib>Séguy, I.</creatorcontrib><creatorcontrib>Temple-Boyer, P.</creatorcontrib><creatorcontrib>Juneau, P.</creatorcontrib><creatorcontrib>Izquierdo, R.</creatorcontrib><creatorcontrib>Launay, J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsopela, A.</au><au>Lale, A.</au><au>Vanhove, E.</au><au>Reynes, O.</au><au>Séguy, I.</au><au>Temple-Boyer, P.</au><au>Juneau, P.</au><au>Izquierdo, R.</au><au>Launay, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Integrated electrochemical biosensor based on algal metabolism for water toxicity analysis</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2014-11-15</date><risdate>2014</risdate><volume>61</volume><spage>290</spage><epage>297</epage><pages>290-297</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>An autonomous electrochemical biosensor with three electrodes integrated on the same silicon chip dedicated to the detection of herbicides in water was fabricated by means of silicon-based microfabrication technology. Platinum (Pt), platinum black (Pt Bl), tungsten/tungsten oxide (W/WO3) and iridium oxide (Pt/IrO2) working ultramicroelectrodes were developed. Ag/AgCl and Pt electrodes were used as reference and counter-integrated electrodes respectively. Physical vapor deposition (PVD) and electrodeposition were used for thin film deposition. The ultramicroelectrodes were employed for the detection of O2, H2O2 and pH related ions H3O+/OH−, species taking part in photosynthetic and metabolic activities of algae. By measuring the variations in consumption–production rates of these electroactive species by algae, the quantity of herbicides present at trace level in the solution can be estimated. Fabricated ultramicroelectrodes were electrochemically characterized and calibrated. Pt Black ultramicroelectrodes exhibited the greatest sensitivity regarding O2 and H2O2 detection while Pt/IrO2 ultramicroelectrodes were more sensitive for pH measurement compared to W/WO3 ultramicroelectrodes for pH measurement. Bioassays were then conducted to detect traces of Diuron herbicide in water samples by evaluating disturbances in photosynthetic and metabolic activities of algae caused by this herbicide.
•This paper presents the development of an electrochemical micro-biosensor for the detection of environmental pollution.•Integrated electrochemical microcells were fabricated.•The electrodes were calibrated and used in bioassays in algal solutions.•O2 evolution was measured in algal solutions.•LOD of 0.2µM for the detection of Diuron herbicide is reported.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>24906088</pmid><doi>10.1016/j.bios.2014.05.004</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Algae Algal metabolism Biological and medical sciences Biosensing Techniques - instrumentation Biosensors Biotechnology Chlamydomonas reinhardtii - metabolism Diuron - analysis Diuron - metabolism Electrochemical Techniques - instrumentation Electrodes Engineering Sciences Equipment Design Fundamental and applied biological sciences. Psychology Herbicides Herbicides - analysis Herbicides - metabolism Hydrogen peroxide Hydrogen Peroxide - analysis Hydrogen Peroxide - metabolism Limit of Detection Methods. Procedures. Technologies Micro and nanotechnologies Microelectrodes Microelectronics Oxygen Oxygen - analysis Oxygen - metabolism Pesticides detection pH measurement Physical vapor deposition Platinum Tungsten oxides Ultramicroelectrodes Various methods and equipments Water Pollutants, Chemical - analysis Water Pollutants, Chemical - metabolism |
| title | Integrated electrochemical biosensor based on algal metabolism for water toxicity analysis |
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