Cellular Response to Linear and Branched Poly(acrylic acid)
Poly(acrylic acid‐co‐sodium acrylate) (PNaA) is a pH‐responsive polymer with potential in anticancer drug delivery. The cytotoxicity and intracellular effects of 3‐arm star, hyperbranched and linear PNaA were investigated with L1210 progenitor leukemia cells and L6 myoblast cells. Free solution capi...
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| Veröffentlicht in: | Macromolecular bioscience 2015-12, Vol.15 (12), p.1724-1734 |
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| creator | Whitty, Elizabeth G. Maniego, Alison R. Bentwitch, Sharon A. Guillaneuf, Yohann Jones, Mark R. Gaborieau, Marianne Castignolles, Patrice |
| description | Poly(acrylic acid‐co‐sodium acrylate) (PNaA) is a pH‐responsive polymer with potential in anticancer drug delivery. The cytotoxicity and intracellular effects of 3‐arm star, hyperbranched and linear PNaA were investigated with L1210 progenitor leukemia cells and L6 myoblast cells. Free solution capillary electrophoresis demonstrated interactions of PNaA with serum proteins. In a 72 h MTT assay most PNaAs exhibited a IC50 between 7 and 14 mmol L−1, showing that precipitation may be a sufficient purification for PNaA dilute solutions. Dialyzed 3‐arm star and hyperbranched PNaA caused an increase in L6 cell viability, challenging the suitability of MTT as cytotoxicity assay for PNaA. Fluorescent confocal microscopy revealed merging of cellular lipids after exposure to PNaA, likely caused by serum starvation.
The cytotoxicity and intracellular effects of poly(acrylic acid)/poly(sodium acrylate) (PNaA) are investigated. This pH‐responsive polymer has potential in anticancer drug delivery. Capillary electrophoresis demonstrates interactions of PNaA with serum proteins. The MTT assay is used to assess the cytotoxicity of PNaA with different branching architectures for L1210 progenitor leukemia cells and L6 myoblast cells. |
| doi_str_mv | 10.1002/mabi.201500153 |
| format | Article |
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The cytotoxicity and intracellular effects of poly(acrylic acid)/poly(sodium acrylate) (PNaA) are investigated. This pH‐responsive polymer has potential in anticancer drug delivery. Capillary electrophoresis demonstrates interactions of PNaA with serum proteins. The MTT assay is used to assess the cytotoxicity of PNaA with different branching architectures for L1210 progenitor leukemia cells and L6 myoblast cells.</description><identifier>ISSN: 1616-5187</identifier><identifier>EISSN: 1616-5195</identifier><identifier>DOI: 10.1002/mabi.201500153</identifier><identifier>PMID: 26257305</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Acrylic Resins - chemistry ; Acrylics ; Animals ; Assaying ; Blood Proteins - chemistry ; Capillarity ; capillary electrophoresis ; cell ; Cell Line, Tumor ; Cell Survival ; Cellular ; Chemical Sciences ; Cytotoxicity ; Drug delivery systems ; Electrophoresis ; Leukemias ; Mice ; MTT assay ; Myoblasts, Skeletal - metabolism ; poly(acrylic acid)/poly(sodium acrylate) ; Serum proteins ; Stars</subject><ispartof>Macromolecular bioscience, 2015-12, Vol.15 (12), p.1724-1734</ispartof><rights>2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6213-29b2fa2315fc7c4557d5e5571bb114e3f9faadebda1503d1eae4252896e7878e3</citedby><cites>FETCH-LOGICAL-c6213-29b2fa2315fc7c4557d5e5571bb114e3f9faadebda1503d1eae4252896e7878e3</cites><orcidid>0000-0002-9991-8123 ; 0000-0002-5390-1553</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmabi.201500153$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmabi.201500153$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26257305$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://amu.hal.science/hal-01460652$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Whitty, Elizabeth G.</creatorcontrib><creatorcontrib>Maniego, Alison R.</creatorcontrib><creatorcontrib>Bentwitch, Sharon A.</creatorcontrib><creatorcontrib>Guillaneuf, Yohann</creatorcontrib><creatorcontrib>Jones, Mark R.</creatorcontrib><creatorcontrib>Gaborieau, Marianne</creatorcontrib><creatorcontrib>Castignolles, Patrice</creatorcontrib><title>Cellular Response to Linear and Branched Poly(acrylic acid)</title><title>Macromolecular bioscience</title><addtitle>Macromol. Biosci</addtitle><description>Poly(acrylic acid‐co‐sodium acrylate) (PNaA) is a pH‐responsive polymer with potential in anticancer drug delivery. The cytotoxicity and intracellular effects of 3‐arm star, hyperbranched and linear PNaA were investigated with L1210 progenitor leukemia cells and L6 myoblast cells. Free solution capillary electrophoresis demonstrated interactions of PNaA with serum proteins. In a 72 h MTT assay most PNaAs exhibited a IC50 between 7 and 14 mmol L−1, showing that precipitation may be a sufficient purification for PNaA dilute solutions. Dialyzed 3‐arm star and hyperbranched PNaA caused an increase in L6 cell viability, challenging the suitability of MTT as cytotoxicity assay for PNaA. Fluorescent confocal microscopy revealed merging of cellular lipids after exposure to PNaA, likely caused by serum starvation.
The cytotoxicity and intracellular effects of poly(acrylic acid)/poly(sodium acrylate) (PNaA) are investigated. This pH‐responsive polymer has potential in anticancer drug delivery. Capillary electrophoresis demonstrates interactions of PNaA with serum proteins. The MTT assay is used to assess the cytotoxicity of PNaA with different branching architectures for L1210 progenitor leukemia cells and L6 myoblast cells.</description><subject>Acrylic Resins - chemistry</subject><subject>Acrylics</subject><subject>Animals</subject><subject>Assaying</subject><subject>Blood Proteins - chemistry</subject><subject>Capillarity</subject><subject>capillary electrophoresis</subject><subject>cell</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival</subject><subject>Cellular</subject><subject>Chemical Sciences</subject><subject>Cytotoxicity</subject><subject>Drug delivery systems</subject><subject>Electrophoresis</subject><subject>Leukemias</subject><subject>Mice</subject><subject>MTT assay</subject><subject>Myoblasts, Skeletal - metabolism</subject><subject>poly(acrylic acid)/poly(sodium acrylate)</subject><subject>Serum proteins</subject><subject>Stars</subject><issn>1616-5187</issn><issn>1616-5195</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctv1DAQxiMEoqXlyhFF4tIesnjs-CVO2xV0i5aWpzhajjNRXbLJ1t4A-9_jVUqEuLQHPzT6zaf55suyF0BmQAh9vbaVn1ECnKTDHmWHIEAUHDR_PP2VPMiexXiTEKk0fZodUEG5ZIQfZm8W2LZDa0P-GeOm7yLm2z5f-Q5TyXZ1fhZs566xzj_27e7EurBrvcut8_XpcfaksW3E53fvUfbt3duvi2Wxujq_WMxXhRMUWEF1RRtLGfDGSVdyLmuO6YaqAiiRNbqxtsaqtskFqwEtlpRTpQVKJRWyo-x01L22rdkEv7ZhZ3rrzXK-MvsagVIQwelPSOzJyG5Cfztg3Jq1jy55tB32QzSgCCmVBsrvR6UiaXmC6geggmgqKdCEvvoPvemH0KX9JIpLLqRU-zFnI-VCH2PAZvIFxOyDNftgzRRsanh5JztUa6wn_G-SCdAj8Mu3uLtHznyYn138K16MvT5u8ffUa8MPIyST3Hy_PDdfyHu1ZBzMJ_YH4ZW5ew</recordid><startdate>201512</startdate><enddate>201512</enddate><creator>Whitty, Elizabeth G.</creator><creator>Maniego, Alison R.</creator><creator>Bentwitch, Sharon A.</creator><creator>Guillaneuf, Yohann</creator><creator>Jones, Mark R.</creator><creator>Gaborieau, Marianne</creator><creator>Castignolles, Patrice</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><general>Wiley-VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>JG9</scope><scope>L7M</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-9991-8123</orcidid><orcidid>https://orcid.org/0000-0002-5390-1553</orcidid></search><sort><creationdate>201512</creationdate><title>Cellular Response to Linear and Branched Poly(acrylic acid)</title><author>Whitty, Elizabeth G. ; Maniego, Alison R. ; Bentwitch, Sharon A. ; Guillaneuf, Yohann ; Jones, Mark R. ; Gaborieau, Marianne ; Castignolles, Patrice</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6213-29b2fa2315fc7c4557d5e5571bb114e3f9faadebda1503d1eae4252896e7878e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acrylic Resins - chemistry</topic><topic>Acrylics</topic><topic>Animals</topic><topic>Assaying</topic><topic>Blood Proteins - chemistry</topic><topic>Capillarity</topic><topic>capillary electrophoresis</topic><topic>cell</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival</topic><topic>Cellular</topic><topic>Chemical Sciences</topic><topic>Cytotoxicity</topic><topic>Drug delivery systems</topic><topic>Electrophoresis</topic><topic>Leukemias</topic><topic>Mice</topic><topic>MTT assay</topic><topic>Myoblasts, Skeletal - metabolism</topic><topic>poly(acrylic acid)/poly(sodium acrylate)</topic><topic>Serum proteins</topic><topic>Stars</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Whitty, Elizabeth G.</creatorcontrib><creatorcontrib>Maniego, Alison R.</creatorcontrib><creatorcontrib>Bentwitch, Sharon A.</creatorcontrib><creatorcontrib>Guillaneuf, Yohann</creatorcontrib><creatorcontrib>Jones, Mark R.</creatorcontrib><creatorcontrib>Gaborieau, Marianne</creatorcontrib><creatorcontrib>Castignolles, Patrice</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Macromolecular bioscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Whitty, Elizabeth G.</au><au>Maniego, Alison R.</au><au>Bentwitch, Sharon A.</au><au>Guillaneuf, Yohann</au><au>Jones, Mark R.</au><au>Gaborieau, Marianne</au><au>Castignolles, Patrice</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellular Response to Linear and Branched Poly(acrylic acid)</atitle><jtitle>Macromolecular bioscience</jtitle><addtitle>Macromol. Biosci</addtitle><date>2015-12</date><risdate>2015</risdate><volume>15</volume><issue>12</issue><spage>1724</spage><epage>1734</epage><pages>1724-1734</pages><issn>1616-5187</issn><eissn>1616-5195</eissn><abstract>Poly(acrylic acid‐co‐sodium acrylate) (PNaA) is a pH‐responsive polymer with potential in anticancer drug delivery. The cytotoxicity and intracellular effects of 3‐arm star, hyperbranched and linear PNaA were investigated with L1210 progenitor leukemia cells and L6 myoblast cells. Free solution capillary electrophoresis demonstrated interactions of PNaA with serum proteins. In a 72 h MTT assay most PNaAs exhibited a IC50 between 7 and 14 mmol L−1, showing that precipitation may be a sufficient purification for PNaA dilute solutions. Dialyzed 3‐arm star and hyperbranched PNaA caused an increase in L6 cell viability, challenging the suitability of MTT as cytotoxicity assay for PNaA. Fluorescent confocal microscopy revealed merging of cellular lipids after exposure to PNaA, likely caused by serum starvation.
The cytotoxicity and intracellular effects of poly(acrylic acid)/poly(sodium acrylate) (PNaA) are investigated. This pH‐responsive polymer has potential in anticancer drug delivery. Capillary electrophoresis demonstrates interactions of PNaA with serum proteins. The MTT assay is used to assess the cytotoxicity of PNaA with different branching architectures for L1210 progenitor leukemia cells and L6 myoblast cells.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>26257305</pmid><doi>10.1002/mabi.201500153</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-9991-8123</orcidid><orcidid>https://orcid.org/0000-0002-5390-1553</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Macromolecular bioscience, 2015-12, Vol.15 (12), p.1724-1734 |
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| source | MEDLINE; Wiley Online Library Journals |
| subjects | Acrylic Resins - chemistry Acrylics Animals Assaying Blood Proteins - chemistry Capillarity capillary electrophoresis cell Cell Line, Tumor Cell Survival Cellular Chemical Sciences Cytotoxicity Drug delivery systems Electrophoresis Leukemias Mice MTT assay Myoblasts, Skeletal - metabolism poly(acrylic acid)/poly(sodium acrylate) Serum proteins Stars |
| title | Cellular Response to Linear and Branched Poly(acrylic acid) |
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