QTL detected in an interspecific pear population confers stable fire blight resistance across different environments and genetic backgrounds
Fire blight, caused by the bacterium Erwinia amylovora (Burrill) Winslow et al., is one of the most serious diseases of pear. The development of pear cultivars with a durable resistance is extremely important for effective control of fire blight and is a key objective of most pear breeding programs...
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| Veröffentlicht in: | Molecular breeding 2016-04, Vol.36 (4), p.1-16, Article 47 |
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| creator | Montanari, Sara Perchepied, Laure Renault, Déborah Frijters, Linda Velasco, Riccardo Horner, Mary Gardiner, Susan E. Chagné, David Bus, Vincent G. M. Durel, Charles-Eric Malnoy, Mickael |
| description | Fire blight, caused by the bacterium Erwinia amylovora (Burrill) Winslow et al., is one of the most serious diseases of pear. The development of pear cultivars with a durable resistance is extremely important for effective control of fire blight and is a key objective of most pear breeding programs throughout the world. We phenotyped seedlings from the interspecific pear population PEAR3 (PremP003, P. × bretschneideri × P. communis) × ‘Moonglow’ (P. communis) for fire blight resistance at two different geographic locations, in France and New Zealand, respectively, employing two local E. amylovora isolates. Using a genetic map constructed with single nucleotide polymorphism (SNP) and microsatellite (SSR) markers previously developed for this segregating population, we detected a major quantitative trait locus (QTL) on linkage group (LG)2 of ‘Moonglow’ (R ² = 12.9–34.4 %), which was stable in both environments. We demonstrated that this QTL co-localizes with another major QTL for fire blight resistance previously detected in ‘Harrow Sweet’ and that the two favorable (i.e., resistant) alleles were not identical by descent. We also identified some smaller effect (R ² = 8.1–14.8 %) QTLs derived from the susceptible parent PEAR3. We propose SNP and SSR markers linked to the large effect QTL on LG2 as candidates for marker-assisted breeding for fire blight resistance in pear. |
| doi_str_mv | 10.1007/s11032-016-0473-z |
| format | Article |
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M. ; Durel, Charles-Eric ; Malnoy, Mickael</creator><creatorcontrib>Montanari, Sara ; Perchepied, Laure ; Renault, Déborah ; Frijters, Linda ; Velasco, Riccardo ; Horner, Mary ; Gardiner, Susan E. ; Chagné, David ; Bus, Vincent G. M. ; Durel, Charles-Eric ; Malnoy, Mickael</creatorcontrib><description>Fire blight, caused by the bacterium Erwinia amylovora (Burrill) Winslow et al., is one of the most serious diseases of pear. The development of pear cultivars with a durable resistance is extremely important for effective control of fire blight and is a key objective of most pear breeding programs throughout the world. We phenotyped seedlings from the interspecific pear population PEAR3 (PremP003, P. × bretschneideri × P. communis) × ‘Moonglow’ (P. communis) for fire blight resistance at two different geographic locations, in France and New Zealand, respectively, employing two local E. amylovora isolates. Using a genetic map constructed with single nucleotide polymorphism (SNP) and microsatellite (SSR) markers previously developed for this segregating population, we detected a major quantitative trait locus (QTL) on linkage group (LG)2 of ‘Moonglow’ (R ² = 12.9–34.4 %), which was stable in both environments. We demonstrated that this QTL co-localizes with another major QTL for fire blight resistance previously detected in ‘Harrow Sweet’ and that the two favorable (i.e., resistant) alleles were not identical by descent. We also identified some smaller effect (R ² = 8.1–14.8 %) QTLs derived from the susceptible parent PEAR3. We propose SNP and SSR markers linked to the large effect QTL on LG2 as candidates for marker-assisted breeding for fire blight resistance in pear.</description><identifier>ISSN: 1380-3743</identifier><identifier>EISSN: 1572-9788</identifier><identifier>DOI: 10.1007/s11032-016-0473-z</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biomedical and Life Sciences ; Biotechnology ; Blight ; Breeding ; Cultivars ; Disease resistance ; Erwinia amylovora ; Fruits ; Gene mapping ; Gene polymorphism ; Geographical locations ; Interspecific ; Life Sciences ; Markers ; Microsatellites ; Molecular biology ; Nucleotides ; Plant biology ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Polymorphism ; Quantitative trait loci ; Seedlings ; Single-nucleotide polymorphism</subject><ispartof>Molecular breeding, 2016-04, Vol.36 (4), p.1-16, Article 47</ispartof><rights>Springer Science+Business Media Dordrecht 2016</rights><rights>Molecular Breeding is a copyright of Springer, (2016). All Rights Reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-4c40863c33c684162904cafe352d45be3181b567bb8671a34e59aa0db48b3fca3</citedby><cites>FETCH-LOGICAL-c407t-4c40863c33c684162904cafe352d45be3181b567bb8671a34e59aa0db48b3fca3</cites><orcidid>0000-0003-3856-2837</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11032-016-0473-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11032-016-0473-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://hal.science/hal-01456003$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Montanari, Sara</creatorcontrib><creatorcontrib>Perchepied, Laure</creatorcontrib><creatorcontrib>Renault, Déborah</creatorcontrib><creatorcontrib>Frijters, Linda</creatorcontrib><creatorcontrib>Velasco, Riccardo</creatorcontrib><creatorcontrib>Horner, Mary</creatorcontrib><creatorcontrib>Gardiner, Susan E.</creatorcontrib><creatorcontrib>Chagné, David</creatorcontrib><creatorcontrib>Bus, Vincent G. M.</creatorcontrib><creatorcontrib>Durel, Charles-Eric</creatorcontrib><creatorcontrib>Malnoy, Mickael</creatorcontrib><title>QTL detected in an interspecific pear population confers stable fire blight resistance across different environments and genetic backgrounds</title><title>Molecular breeding</title><addtitle>Mol Breeding</addtitle><description>Fire blight, caused by the bacterium Erwinia amylovora (Burrill) Winslow et al., is one of the most serious diseases of pear. The development of pear cultivars with a durable resistance is extremely important for effective control of fire blight and is a key objective of most pear breeding programs throughout the world. We phenotyped seedlings from the interspecific pear population PEAR3 (PremP003, P. × bretschneideri × P. communis) × ‘Moonglow’ (P. communis) for fire blight resistance at two different geographic locations, in France and New Zealand, respectively, employing two local E. amylovora isolates. Using a genetic map constructed with single nucleotide polymorphism (SNP) and microsatellite (SSR) markers previously developed for this segregating population, we detected a major quantitative trait locus (QTL) on linkage group (LG)2 of ‘Moonglow’ (R ² = 12.9–34.4 %), which was stable in both environments. We demonstrated that this QTL co-localizes with another major QTL for fire blight resistance previously detected in ‘Harrow Sweet’ and that the two favorable (i.e., resistant) alleles were not identical by descent. We also identified some smaller effect (R ² = 8.1–14.8 %) QTLs derived from the susceptible parent PEAR3. We propose SNP and SSR markers linked to the large effect QTL on LG2 as candidates for marker-assisted breeding for fire blight resistance in pear.</description><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Blight</subject><subject>Breeding</subject><subject>Cultivars</subject><subject>Disease resistance</subject><subject>Erwinia amylovora</subject><subject>Fruits</subject><subject>Gene mapping</subject><subject>Gene polymorphism</subject><subject>Geographical locations</subject><subject>Interspecific</subject><subject>Life Sciences</subject><subject>Markers</subject><subject>Microsatellites</subject><subject>Molecular biology</subject><subject>Nucleotides</subject><subject>Plant biology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Polymorphism</subject><subject>Quantitative trait loci</subject><subject>Seedlings</subject><subject>Single-nucleotide polymorphism</subject><issn>1380-3743</issn><issn>1572-9788</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kc1u1TAQhSNEJUrpA3SFJTawCNjxb5ZVBS3SlRBqu7YcZ5y65NrBdirRZ-Ch60sQSCzYeEbj75wZe5rmjOD3BGP5IROCaddiIlrMJG0fnzXHhMuu7aVSz2tOFW6pZPRF8zLne1w1vRDHzc-vNzs0QgFbYEQ-IBPqWSDlBax33qIFTEJLXNbZFB8DsjG4eo1yMcMMyPkEaJj9dFdQguxrOVhAxqaYMxq9qzCEgiA8-BTDvua5NhnRBAFK9R-M_TaluIYxv2qOnJkznP6OJ83tp483F1ft7svl54vzXWsZlqVlNShBLaVWKEZE12NmjQPKu5HxAShRZOBCDoMSkhjKgPfG4HFgaqDOGnrSvNt878ysl-T3Jv3Q0Xh9db7ThxomjAuM6QOp7NuNXVL8vkIueu-zhXk2AeKaNVFCUSwpYxV98w96H9cU6kt01_Ge9Ur2faXIRv36oQTuzwQE68Mu9bbLOoTQh13qx6rpNk2ubJgg_XX-n-j1JnImajMln_XtdVcBjAnlgnD6BFUYrJ4</recordid><startdate>20160401</startdate><enddate>20160401</enddate><creator>Montanari, Sara</creator><creator>Perchepied, Laure</creator><creator>Renault, Déborah</creator><creator>Frijters, Linda</creator><creator>Velasco, Riccardo</creator><creator>Horner, Mary</creator><creator>Gardiner, Susan E.</creator><creator>Chagné, David</creator><creator>Bus, Vincent G. 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M.</au><au>Durel, Charles-Eric</au><au>Malnoy, Mickael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>QTL detected in an interspecific pear population confers stable fire blight resistance across different environments and genetic backgrounds</atitle><jtitle>Molecular breeding</jtitle><stitle>Mol Breeding</stitle><date>2016-04-01</date><risdate>2016</risdate><volume>36</volume><issue>4</issue><spage>1</spage><epage>16</epage><pages>1-16</pages><artnum>47</artnum><issn>1380-3743</issn><eissn>1572-9788</eissn><abstract>Fire blight, caused by the bacterium Erwinia amylovora (Burrill) Winslow et al., is one of the most serious diseases of pear. The development of pear cultivars with a durable resistance is extremely important for effective control of fire blight and is a key objective of most pear breeding programs throughout the world. We phenotyped seedlings from the interspecific pear population PEAR3 (PremP003, P. × bretschneideri × P. communis) × ‘Moonglow’ (P. communis) for fire blight resistance at two different geographic locations, in France and New Zealand, respectively, employing two local E. amylovora isolates. Using a genetic map constructed with single nucleotide polymorphism (SNP) and microsatellite (SSR) markers previously developed for this segregating population, we detected a major quantitative trait locus (QTL) on linkage group (LG)2 of ‘Moonglow’ (R ² = 12.9–34.4 %), which was stable in both environments. We demonstrated that this QTL co-localizes with another major QTL for fire blight resistance previously detected in ‘Harrow Sweet’ and that the two favorable (i.e., resistant) alleles were not identical by descent. We also identified some smaller effect (R ² = 8.1–14.8 %) QTLs derived from the susceptible parent PEAR3. We propose SNP and SSR markers linked to the large effect QTL on LG2 as candidates for marker-assisted breeding for fire blight resistance in pear.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11032-016-0473-z</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0003-3856-2837</orcidid></addata></record> |
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| ispartof | Molecular breeding, 2016-04, Vol.36 (4), p.1-16, Article 47 |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Biomedical and Life Sciences Biotechnology Blight Breeding Cultivars Disease resistance Erwinia amylovora Fruits Gene mapping Gene polymorphism Geographical locations Interspecific Life Sciences Markers Microsatellites Molecular biology Nucleotides Plant biology Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Polymorphism Quantitative trait loci Seedlings Single-nucleotide polymorphism |
| title | QTL detected in an interspecific pear population confers stable fire blight resistance across different environments and genetic backgrounds |
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