Digital antimicrobial susceptibility testing using the MilliDrop technology
We present the MilliDrop Analyzer (MDA), a droplet-based millifluidic system for digital antimicrobial susceptibility testing (D-AST), which enables us to determine minimum inhibitory concentrations (MICs) precisely and accurately. The MilliDrop technology was validated by using resazurin for fluore...
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| Veröffentlicht in: | European journal of clinical microbiology & infectious diseases 2016-03, Vol.35 (3), p.415-422 |
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| creator | Jiang, L. Boitard, L. Broyer, P. Chareire, A.-C. Bourne-Branchu, P. Mahé, P. Tournoud, M. Franceschi, C. Zambardi, G. Baudry, J. Bibette, J. |
| description | We present the MilliDrop Analyzer (MDA), a droplet-based millifluidic system for digital antimicrobial susceptibility testing (D-AST), which enables us to determine minimum inhibitory concentrations (MICs) precisely and accurately. The MilliDrop technology was validated by using resazurin for fluorescence readout, for comparison with standard methodology, and for conducting reproducibility studies. In this first assessment, the susceptibility of a reference Gram-negative strain
Escherichia coli
ATCC 25922 to gentamicin, chloramphenicol, and nalidixic acid were tested by the MDA, VITEK®2, and broth microdilution as a reference standard. We measured the susceptibility of clinically relevant Gram-positive strains of
Staphylococcus aureus
to vancomycin, including vancomycin-intermediate
S. aureus
(VISA), heterogeneous vancomycin-intermediate
S. aureus
(hVISA), and vancomycin-susceptible
S. aureus
(VSSA) strains. The MDA provided results which were much more accurate than those of VITEK®2 and standard broth microdilution. The enhanced accuracy enabled us to reliably discriminate between VSSA and hVISA strains. |
| doi_str_mv | 10.1007/s10096-015-2554-z |
| format | Article |
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Escherichia coli
ATCC 25922 to gentamicin, chloramphenicol, and nalidixic acid were tested by the MDA, VITEK®2, and broth microdilution as a reference standard. We measured the susceptibility of clinically relevant Gram-positive strains of
Staphylococcus aureus
to vancomycin, including vancomycin-intermediate
S. aureus
(VISA), heterogeneous vancomycin-intermediate
S. aureus
(hVISA), and vancomycin-susceptible
S. aureus
(VSSA) strains. The MDA provided results which were much more accurate than those of VITEK®2 and standard broth microdilution. The enhanced accuracy enabled us to reliably discriminate between VSSA and hVISA strains.</description><identifier>ISSN: 0934-9723</identifier><identifier>EISSN: 1435-4373</identifier><identifier>DOI: 10.1007/s10096-015-2554-z</identifier><identifier>PMID: 26803821</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Anti-Infective Agents - pharmacology ; Antibiotics ; Bacterial infections ; Biomedical and Life Sciences ; Biomedicine ; Condensed Matter ; E coli ; Escherichia coli ; Humans ; Internal Medicine ; Medical Microbiology ; Microbial Sensitivity Tests - instrumentation ; Microbial Sensitivity Tests - methods ; Miniaturization - instrumentation ; Miniaturization - methods ; Original Article ; Physics ; Soft Condensed Matter ; Staphylococcus aureus ; Yeast</subject><ispartof>European journal of clinical microbiology & infectious diseases, 2016-03, Vol.35 (3), p.415-422</ispartof><rights>Springer-Verlag Berlin Heidelberg 2016</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-c4e1f5fe8f01d06f60a1b575230094530c50a905da8ec2a0ba457ef51063edd33</citedby><cites>FETCH-LOGICAL-c509t-c4e1f5fe8f01d06f60a1b575230094530c50a905da8ec2a0ba457ef51063edd33</cites><orcidid>0000-0003-2614-5120 ; 0000-0001-6111-7948 ; 0000-0002-5526-499X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10096-015-2554-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10096-015-2554-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26803821$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01400797$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, L.</creatorcontrib><creatorcontrib>Boitard, L.</creatorcontrib><creatorcontrib>Broyer, P.</creatorcontrib><creatorcontrib>Chareire, A.-C.</creatorcontrib><creatorcontrib>Bourne-Branchu, P.</creatorcontrib><creatorcontrib>Mahé, P.</creatorcontrib><creatorcontrib>Tournoud, M.</creatorcontrib><creatorcontrib>Franceschi, C.</creatorcontrib><creatorcontrib>Zambardi, G.</creatorcontrib><creatorcontrib>Baudry, J.</creatorcontrib><creatorcontrib>Bibette, J.</creatorcontrib><title>Digital antimicrobial susceptibility testing using the MilliDrop technology</title><title>European journal of clinical microbiology & infectious diseases</title><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><description>We present the MilliDrop Analyzer (MDA), a droplet-based millifluidic system for digital antimicrobial susceptibility testing (D-AST), which enables us to determine minimum inhibitory concentrations (MICs) precisely and accurately. The MilliDrop technology was validated by using resazurin for fluorescence readout, for comparison with standard methodology, and for conducting reproducibility studies. In this first assessment, the susceptibility of a reference Gram-negative strain
Escherichia coli
ATCC 25922 to gentamicin, chloramphenicol, and nalidixic acid were tested by the MDA, VITEK®2, and broth microdilution as a reference standard. We measured the susceptibility of clinically relevant Gram-positive strains of
Staphylococcus aureus
to vancomycin, including vancomycin-intermediate
S. aureus
(VISA), heterogeneous vancomycin-intermediate
S. aureus
(hVISA), and vancomycin-susceptible
S. aureus
(VSSA) strains. The MDA provided results which were much more accurate than those of VITEK®2 and standard broth microdilution. The enhanced accuracy enabled us to reliably discriminate between VSSA and hVISA strains.</description><subject>Anti-Infective Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Bacterial infections</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Condensed Matter</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Medical Microbiology</subject><subject>Microbial Sensitivity Tests - instrumentation</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Miniaturization - instrumentation</subject><subject>Miniaturization - methods</subject><subject>Original Article</subject><subject>Physics</subject><subject>Soft Condensed Matter</subject><subject>Staphylococcus aureus</subject><subject>Yeast</subject><issn>0934-9723</issn><issn>1435-4373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkU1LxDAQhoMo7vrxA7zIghc9VCdN0rTHZf1YccWLnkPapt0s2XZtWmH99U6pigiClwnJPPPOvBlCTihcUgB55TEmUQBUBKEQPHjfIWPKmQg4k2yXjCFhPEhkyEbkwPsVYE0s5T4ZhVEMLA7pmDxc29K22k101dq1zZo6tXjznc_MprWpdbbdTlrjW1uVk873sV2ayaN1zl439QZz2bKqXV1uj8heoZ03x5_nIXm5vXmezYPF0939bLoIMgFJG2Tc0EIUJi6A5hAVEWiaCilChm64YICYTkDkOjZZqCHVXEhTCAoRM3nO2CG5GHSX2qlNY9e62apaWzWfLlT_BpSj1US-UWTPB3bT1K8d2lBri9ac05WpO6-ojLExxugfaCQTil_XT3D2C13VXVOhaaQkDhoJ3gvSgcJf9b4xxfewFFS_QDUsEOcVql-gesea00_lLl2b_Lvia2MIhAPgMVWVpvnR-k_VD3f0pM8</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>Jiang, L.</creator><creator>Boitard, L.</creator><creator>Broyer, P.</creator><creator>Chareire, A.-C.</creator><creator>Bourne-Branchu, P.</creator><creator>Mahé, P.</creator><creator>Tournoud, M.</creator><creator>Franceschi, C.</creator><creator>Zambardi, G.</creator><creator>Baudry, J.</creator><creator>Bibette, J.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><general>Springer Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-2614-5120</orcidid><orcidid>https://orcid.org/0000-0001-6111-7948</orcidid><orcidid>https://orcid.org/0000-0002-5526-499X</orcidid></search><sort><creationdate>20160301</creationdate><title>Digital antimicrobial susceptibility testing using the MilliDrop technology</title><author>Jiang, L. ; Boitard, L. ; Broyer, P. ; Chareire, A.-C. ; Bourne-Branchu, P. ; Mahé, P. ; Tournoud, M. ; Franceschi, C. ; Zambardi, G. ; Baudry, J. ; Bibette, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c509t-c4e1f5fe8f01d06f60a1b575230094530c50a905da8ec2a0ba457ef51063edd33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Anti-Infective Agents - 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Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>European journal of clinical microbiology & infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, L.</au><au>Boitard, L.</au><au>Broyer, P.</au><au>Chareire, A.-C.</au><au>Bourne-Branchu, P.</au><au>Mahé, P.</au><au>Tournoud, M.</au><au>Franceschi, C.</au><au>Zambardi, G.</au><au>Baudry, J.</au><au>Bibette, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Digital antimicrobial susceptibility testing using the MilliDrop technology</atitle><jtitle>European journal of clinical microbiology & infectious diseases</jtitle><stitle>Eur J Clin Microbiol Infect Dis</stitle><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>35</volume><issue>3</issue><spage>415</spage><epage>422</epage><pages>415-422</pages><issn>0934-9723</issn><eissn>1435-4373</eissn><abstract>We present the MilliDrop Analyzer (MDA), a droplet-based millifluidic system for digital antimicrobial susceptibility testing (D-AST), which enables us to determine minimum inhibitory concentrations (MICs) precisely and accurately. The MilliDrop technology was validated by using resazurin for fluorescence readout, for comparison with standard methodology, and for conducting reproducibility studies. In this first assessment, the susceptibility of a reference Gram-negative strain
Escherichia coli
ATCC 25922 to gentamicin, chloramphenicol, and nalidixic acid were tested by the MDA, VITEK®2, and broth microdilution as a reference standard. We measured the susceptibility of clinically relevant Gram-positive strains of
Staphylococcus aureus
to vancomycin, including vancomycin-intermediate
S. aureus
(VISA), heterogeneous vancomycin-intermediate
S. aureus
(hVISA), and vancomycin-susceptible
S. aureus
(VSSA) strains. The MDA provided results which were much more accurate than those of VITEK®2 and standard broth microdilution. The enhanced accuracy enabled us to reliably discriminate between VSSA and hVISA strains.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26803821</pmid><doi>10.1007/s10096-015-2554-z</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-2614-5120</orcidid><orcidid>https://orcid.org/0000-0001-6111-7948</orcidid><orcidid>https://orcid.org/0000-0002-5526-499X</orcidid></addata></record> |
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| subjects | Anti-Infective Agents - pharmacology Antibiotics Bacterial infections Biomedical and Life Sciences Biomedicine Condensed Matter E coli Escherichia coli Humans Internal Medicine Medical Microbiology Microbial Sensitivity Tests - instrumentation Microbial Sensitivity Tests - methods Miniaturization - instrumentation Miniaturization - methods Original Article Physics Soft Condensed Matter Staphylococcus aureus Yeast |
| title | Digital antimicrobial susceptibility testing using the MilliDrop technology |
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