An electrochemical ELISA-like immunosensor for miRNAs detection based on screen-printed gold electrodes modified with reduced graphene oxide and carbon nanotubes
We design an electrochemical immunosensor for miRNA detection, based on screen-printed gold electrodes modified with reduced graphene oxide and carbon nanotubes. An original immunological approach is followed, using antibodies directed to DNA.RNA hybrids. An electrochemical ELISA-like amplification...
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| Veröffentlicht in: | Biosensors & bioelectronics 2014-12, Vol.62, p.25-30 |
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| container_title | Biosensors & bioelectronics |
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| creator | Tran, H.V. Piro, B. Reisberg, S. Huy Nguyen, L. Dung Nguyen, T. Duc, H.T. Pham, M.C. |
| description | We design an electrochemical immunosensor for miRNA detection, based on screen-printed gold electrodes modified with reduced graphene oxide and carbon nanotubes. An original immunological approach is followed, using antibodies directed to DNA.RNA hybrids. An electrochemical ELISA-like amplification strategy was set up using a secondary antibody conjugated to horseradish peroxidase (HRP). Hydroquinone is oxidized into benzoquinone by the HRP/H2O2 catalytic system. In turn, benzoquinone is electroreduced into hydroquinone at the electrode. The catalytic reduction current is related to HRP amount immobilized on the surface, which itself is related to miRNA.DNA surface density on the electrode. This architecture, compared to classical optical detection, lowers the detection limit down to 10fM. Two miRNAs were studied: miR-141 (a prostate biomarker) and miR-29b-1 (a lung cancer biomarker).
Schematic representation of the electrochemical ELISA-like immunosensor.
[Display omitted]
•ELISA-like electrochemical immunodetection of miRNA.•Reduced graphene oxide and carbon nanotubes composite.•Antibodies specific to DNA.RNA hybrids. |
| doi_str_mv | 10.1016/j.bios.2014.06.014 |
| format | Article |
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Schematic representation of the electrochemical ELISA-like immunosensor.
[Display omitted]
•ELISA-like electrochemical immunodetection of miRNA.•Reduced graphene oxide and carbon nanotubes composite.•Antibodies specific to DNA.RNA hybrids.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2014.06.014</identifier><identifier>PMID: 24973539</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Antibodies ; Benzoquinone ; Biological and medical sciences ; Biosensors ; Biotechnology ; Carbon nanotubes ; Catalysis ; Catalysts ; Chemical Sciences ; Electrochemical biosensor ; Electrochemical Techniques - instrumentation ; Electrodes ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; Gold - chemistry ; Graphene ; Graphite - chemistry ; Immunoassay - instrumentation ; Immunodetection ; Label-free detection ; Limit of Detection ; Methods. Procedures. Technologies ; MicroRNAs - analysis ; miRNA ; Nanotubes, Carbon - chemistry ; Oxidation-Reduction ; Oxides ; Oxides - chemistry ; Reduced graphene oxide ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 2014-12, Vol.62, p.25-30</ispartof><rights>2014 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c523t-c88d470328540e77c55dc5d12e1913cc9a416ff64f8c98b3d33d719144960b833</citedby><cites>FETCH-LOGICAL-c523t-c88d470328540e77c55dc5d12e1913cc9a416ff64f8c98b3d33d719144960b833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0956566314004278$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28640302$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24973539$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01326600$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Tran, H.V.</creatorcontrib><creatorcontrib>Piro, B.</creatorcontrib><creatorcontrib>Reisberg, S.</creatorcontrib><creatorcontrib>Huy Nguyen, L.</creatorcontrib><creatorcontrib>Dung Nguyen, T.</creatorcontrib><creatorcontrib>Duc, H.T.</creatorcontrib><creatorcontrib>Pham, M.C.</creatorcontrib><title>An electrochemical ELISA-like immunosensor for miRNAs detection based on screen-printed gold electrodes modified with reduced graphene oxide and carbon nanotubes</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>We design an electrochemical immunosensor for miRNA detection, based on screen-printed gold electrodes modified with reduced graphene oxide and carbon nanotubes. An original immunological approach is followed, using antibodies directed to DNA.RNA hybrids. An electrochemical ELISA-like amplification strategy was set up using a secondary antibody conjugated to horseradish peroxidase (HRP). Hydroquinone is oxidized into benzoquinone by the HRP/H2O2 catalytic system. In turn, benzoquinone is electroreduced into hydroquinone at the electrode. The catalytic reduction current is related to HRP amount immobilized on the surface, which itself is related to miRNA.DNA surface density on the electrode. This architecture, compared to classical optical detection, lowers the detection limit down to 10fM. Two miRNAs were studied: miR-141 (a prostate biomarker) and miR-29b-1 (a lung cancer biomarker).
Schematic representation of the electrochemical ELISA-like immunosensor.
[Display omitted]
•ELISA-like electrochemical immunodetection of miRNA.•Reduced graphene oxide and carbon nanotubes composite.•Antibodies specific to DNA.RNA hybrids.</description><subject>Antibodies</subject><subject>Benzoquinone</subject><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Carbon nanotubes</subject><subject>Catalysis</subject><subject>Catalysts</subject><subject>Chemical Sciences</subject><subject>Electrochemical biosensor</subject><subject>Electrochemical Techniques - instrumentation</subject><subject>Electrodes</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gold - chemistry</subject><subject>Graphene</subject><subject>Graphite - chemistry</subject><subject>Immunoassay - instrumentation</subject><subject>Immunodetection</subject><subject>Label-free detection</subject><subject>Limit of Detection</subject><subject>Methods. Procedures. Technologies</subject><subject>MicroRNAs - analysis</subject><subject>miRNA</subject><subject>Nanotubes, Carbon - chemistry</subject><subject>Oxidation-Reduction</subject><subject>Oxides</subject><subject>Oxides - chemistry</subject><subject>Reduced graphene oxide</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk1v1DAQhiMEokvhD3BAviDBIcs4_kgicYmqQiutQOLjbDn2hPWS2IudFPg5_FO82m25AQdrrPEz47HftyieUlhToPLVbt27kNYVUL4Guc7hXrGiTc1KXjFxv1hBK2QppGRnxaOUdgBQ0xYeFmcVb2smWLsqfnWe4IhmjsFscXJGj-Ryc_2xK0f3FYmbpsWHhD6FSIa8JvfhXZeIxTnXuOBJrxNakjfJRERf7qPzc858CaO97WwxkSlYN7h88N3NWxLRLuZARb3fokcSfjiLRHtLjI59bue1D_PSY3pcPBj0mPDJKZ4Xn99cfrq4Kjfv315fdJvSiIrNpWkay2tgVSM4YF0bIawRllZIW8qMaTWnchgkHxrTNj2zjNn8G5TzVkLfMHZevDz23epR5VdMOv5UQTt11W3UIQeUVVIC3NDMvjiy-xi-LZhmNblkcBy1x7AkRWVWABoQ7D_Qqm5rClz8GxW8kZxmbTNaHVETQ0oRh7uJKaiDOdROHcyhDuZQIPPwPBc9O_Vf-gntXcmtGzLw_ATolI0wRO2NS3-4fDswqDL3-shh1uPGYVTJOPRZUBez4MoG97c5fgM14NfA</recordid><startdate>20141215</startdate><enddate>20141215</enddate><creator>Tran, H.V.</creator><creator>Piro, B.</creator><creator>Reisberg, S.</creator><creator>Huy Nguyen, L.</creator><creator>Dung Nguyen, T.</creator><creator>Duc, H.T.</creator><creator>Pham, M.C.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SP</scope><scope>7SR</scope><scope>7U5</scope><scope>JG9</scope><scope>L7M</scope><scope>1XC</scope></search><sort><creationdate>20141215</creationdate><title>An electrochemical ELISA-like immunosensor for miRNAs detection based on screen-printed gold electrodes modified with reduced graphene oxide and carbon nanotubes</title><author>Tran, H.V. ; Piro, B. ; Reisberg, S. ; Huy Nguyen, L. ; Dung Nguyen, T. ; Duc, H.T. ; Pham, M.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c523t-c88d470328540e77c55dc5d12e1913cc9a416ff64f8c98b3d33d719144960b833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Antibodies</topic><topic>Benzoquinone</topic><topic>Biological and medical sciences</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Carbon nanotubes</topic><topic>Catalysis</topic><topic>Catalysts</topic><topic>Chemical Sciences</topic><topic>Electrochemical biosensor</topic><topic>Electrochemical Techniques - instrumentation</topic><topic>Electrodes</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gold - chemistry</topic><topic>Graphene</topic><topic>Graphite - chemistry</topic><topic>Immunoassay - instrumentation</topic><topic>Immunodetection</topic><topic>Label-free detection</topic><topic>Limit of Detection</topic><topic>Methods. Procedures. Technologies</topic><topic>MicroRNAs - analysis</topic><topic>miRNA</topic><topic>Nanotubes, Carbon - chemistry</topic><topic>Oxidation-Reduction</topic><topic>Oxides</topic><topic>Oxides - chemistry</topic><topic>Reduced graphene oxide</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tran, H.V.</creatorcontrib><creatorcontrib>Piro, B.</creatorcontrib><creatorcontrib>Reisberg, S.</creatorcontrib><creatorcontrib>Huy Nguyen, L.</creatorcontrib><creatorcontrib>Dung Nguyen, T.</creatorcontrib><creatorcontrib>Duc, H.T.</creatorcontrib><creatorcontrib>Pham, M.C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tran, H.V.</au><au>Piro, B.</au><au>Reisberg, S.</au><au>Huy Nguyen, L.</au><au>Dung Nguyen, T.</au><au>Duc, H.T.</au><au>Pham, M.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An electrochemical ELISA-like immunosensor for miRNAs detection based on screen-printed gold electrodes modified with reduced graphene oxide and carbon nanotubes</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2014-12-15</date><risdate>2014</risdate><volume>62</volume><spage>25</spage><epage>30</epage><pages>25-30</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>We design an electrochemical immunosensor for miRNA detection, based on screen-printed gold electrodes modified with reduced graphene oxide and carbon nanotubes. An original immunological approach is followed, using antibodies directed to DNA.RNA hybrids. An electrochemical ELISA-like amplification strategy was set up using a secondary antibody conjugated to horseradish peroxidase (HRP). Hydroquinone is oxidized into benzoquinone by the HRP/H2O2 catalytic system. In turn, benzoquinone is electroreduced into hydroquinone at the electrode. The catalytic reduction current is related to HRP amount immobilized on the surface, which itself is related to miRNA.DNA surface density on the electrode. This architecture, compared to classical optical detection, lowers the detection limit down to 10fM. Two miRNAs were studied: miR-141 (a prostate biomarker) and miR-29b-1 (a lung cancer biomarker).
Schematic representation of the electrochemical ELISA-like immunosensor.
[Display omitted]
•ELISA-like electrochemical immunodetection of miRNA.•Reduced graphene oxide and carbon nanotubes composite.•Antibodies specific to DNA.RNA hybrids.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>24973539</pmid><doi>10.1016/j.bios.2014.06.014</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biosensors & bioelectronics, 2014-12, Vol.62, p.25-30 |
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| subjects | Antibodies Benzoquinone Biological and medical sciences Biosensors Biotechnology Carbon nanotubes Catalysis Catalysts Chemical Sciences Electrochemical biosensor Electrochemical Techniques - instrumentation Electrodes Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Gold - chemistry Graphene Graphite - chemistry Immunoassay - instrumentation Immunodetection Label-free detection Limit of Detection Methods. Procedures. Technologies MicroRNAs - analysis miRNA Nanotubes, Carbon - chemistry Oxidation-Reduction Oxides Oxides - chemistry Reduced graphene oxide Various methods and equipments |
| title | An electrochemical ELISA-like immunosensor for miRNAs detection based on screen-printed gold electrodes modified with reduced graphene oxide and carbon nanotubes |
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