A new light on the meiotic DSB catalytic complex
Meiotic recombination is initiated by the formation of programmed DNA double-strand breaks (DSBs). More than 15 years ago, Spo11 was identified as the protein responsible for meiotic DSB formation, notably because of its striking similarities with the A subunit of topoisomerase VI (TopoVI). TopoVI a...
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| Veröffentlicht in: | Seminars in cell & developmental biology 2016-06, Vol.54, p.165-176 |
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| creator | Robert, Thomas Vrielynck, Nathalie Mézard, Christine de Massy, Bernard Grelon, Mathilde |
| description | Meiotic recombination is initiated by the formation of programmed DNA double-strand breaks (DSBs). More than 15 years ago, Spo11 was identified as the protein responsible for meiotic DSB formation, notably because of its striking similarities with the A subunit of topoisomerase VI (TopoVI). TopoVI are enzymes that modify DNA topology by generating transient DSBs and are active as heterotetramers, composed of two A and two B subunits. A2 dimers catalyse the DNA cleavage reaction, whereas the B subunits regulate A2 conformation, DNA capture, cleavage and re-ligation. The recent identification in plants and mammals of a B-like TopoVI subunit that interacts with SPO11 and is required for meiotic DSB formation makes us to reconsider our understanding of the meiotic DSB catalytic complex. We provide here an overview of the knowledge on TopoVI structure and mode of action and we compare them with their meiotic counterparts. This allows us to discuss the nature, structure and functions of the meiotic TopoVI-like complex during meiotic DSB formation. |
| doi_str_mv | 10.1016/j.semcdb.2016.02.025 |
| format | Article |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biocatalysis DNA Breaks, Double-Stranded Double strand break Endodeoxyribonucleases - metabolism Enzymes - metabolism Genetics Life Sciences Meiosis Models, Biological Recombination SPO11 Topoisomerase |
| title | A new light on the meiotic DSB catalytic complex |
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