Genetic diversity of the endemic honeybee: Apis mellifera unicolor (Hymenoptera: Apidae) in Madagascar
Apis mellifera unicolor is a tropical honeybee endemic of Madagascar. Comprehensive knowledge about its mitochondrial and nuclear genetic diversity and structuration was our main purpose. Samples of worker bees were collected from 867 colonies in 76 sites in Madagascar and 1 reference population in...
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| Veröffentlicht in: | Apidologie 2015-11, Vol.46 (6), p.735-747 |
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| container_title | Apidologie |
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| creator | Rasolofoarivao, Henriette Clémencet, Johanna Techer, Maéva Angélique Ravaomanarivo, Lala Harivelo Raveloson Reynaud, Bernard Delatte, Hélène |
| description | Apis mellifera unicolor is a tropical honeybee endemic of Madagascar. Comprehensive knowledge about its mitochondrial and nuclear genetic diversity and structuration was our main purpose. Samples of worker bees were collected from 867 colonies in 76 sites in Madagascar and 1 reference population in South Africa. PCR-restriction fragment length polymorphism (RFLP) and sequencing were used to reveal variability in the COI–COII mtDNA region. Seventeen microsatellite loci were used for studying the nuclear diversity. Three PCR-RFLP profiles were observed, among which 99.4 % belonged to A1 haplotype, 0.2 % to a new A haplotype, and 0.4 % to A4 haplotype. In microsatellite analysis, moderate genetic diversity values were found for Madagascar, together with low mean number of alleles ranging from 2.47 to 3.88 compared to South Africa. Bayesian clustering assignment methods and principal component analysis (PCA) separated populations into two genetic clusters which matched with geographic areas. Several hypotheses are discussed regarding to the low genetic diversity of A. m. unicolor in its native range. |
| doi_str_mv | 10.1007/s13592-015-0362-1 |
| format | Article |
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Comprehensive knowledge about its mitochondrial and nuclear genetic diversity and structuration was our main purpose. Samples of worker bees were collected from 867 colonies in 76 sites in Madagascar and 1 reference population in South Africa. PCR-restriction fragment length polymorphism (RFLP) and sequencing were used to reveal variability in the COI–COII mtDNA region. Seventeen microsatellite loci were used for studying the nuclear diversity. Three PCR-RFLP profiles were observed, among which 99.4 % belonged to A1 haplotype, 0.2 % to a new A haplotype, and 0.4 % to A4 haplotype. In microsatellite analysis, moderate genetic diversity values were found for Madagascar, together with low mean number of alleles ranging from 2.47 to 3.88 compared to South Africa. Bayesian clustering assignment methods and principal component analysis (PCA) separated populations into two genetic clusters which matched with geographic areas. 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Comprehensive knowledge about its mitochondrial and nuclear genetic diversity and structuration was our main purpose. Samples of worker bees were collected from 867 colonies in 76 sites in Madagascar and 1 reference population in South Africa. PCR-restriction fragment length polymorphism (RFLP) and sequencing were used to reveal variability in the COI–COII mtDNA region. Seventeen microsatellite loci were used for studying the nuclear diversity. Three PCR-RFLP profiles were observed, among which 99.4 % belonged to A1 haplotype, 0.2 % to a new A haplotype, and 0.4 % to A4 haplotype. In microsatellite analysis, moderate genetic diversity values were found for Madagascar, together with low mean number of alleles ranging from 2.47 to 3.88 compared to South Africa. Bayesian clustering assignment methods and principal component analysis (PCA) separated populations into two genetic clusters which matched with geographic areas. Several hypotheses are discussed regarding to the low genetic diversity of A. m. unicolor in its native range.</description><subject>alleles</subject><subject>Apis mellifera</subject><subject>Biomedical and Life Sciences</subject><subject>Entomology</subject><subject>genetic variation</subject><subject>haplotypes</subject><subject>honey bees</subject><subject>Life Sciences</subject><subject>microsatellite repeats</subject><subject>mitochondrial DNA</subject><subject>Original Article</subject><subject>polymerase chain reaction</subject><subject>population</subject><subject>principal component analysis</subject><subject>restriction fragment length polymorphism</subject><subject>worker bees</subject><issn>0044-8435</issn><issn>1297-9678</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNp9kTFv2zAQhYmgAeIm_QGdyrEelN6RFCV2M4w0DuAiQ5KZoKWjzUAWDVIJ4H8fqSo6djrg3fsOeO8Y-4pwiwDVj4yyNKIALAuQWhR4wRYoTFUYXdWf2AJAqaJWsrxin3N-BUBRl2rB_D31NISGt-GdUg7DmUfPhwNx6ls6jotD7Om8I_rJV6eQ-ZG6LnhKjr_1oYldTPz75nykPp6GUf3jah0teej5b9e6vcuNSzfs0rsu05e_85q9_Lp7Xm-K7eP9w3q1LRpZm6HQUkEtNSrVSqyN9NjQjrQ3pm1dJTTo0qHyWDVip2kHjWjr0nmFtavGbFJes-V89-A6e0rh6NLZRhfsZrW1kwYoQYjSvOPoxdnbpJhzIv8PQLBTqXYudWRKO5VqJ0bMTB69_Z6SfY1vqR8j_Rf6NkPeRev2KWT78iQANYxfqYxS8gM0hoIi</recordid><startdate>20151101</startdate><enddate>20151101</enddate><creator>Rasolofoarivao, Henriette</creator><creator>Clémencet, Johanna</creator><creator>Techer, Maéva Angélique</creator><creator>Ravaomanarivo, Lala Harivelo Raveloson</creator><creator>Reynaud, Bernard</creator><creator>Delatte, Hélène</creator><general>Springer Paris</general><general>Springer Verlag</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>1XC</scope><scope>VOOES</scope></search><sort><creationdate>20151101</creationdate><title>Genetic diversity of the endemic honeybee: Apis mellifera unicolor (Hymenoptera: Apidae) in Madagascar</title><author>Rasolofoarivao, Henriette ; Clémencet, Johanna ; Techer, Maéva Angélique ; Ravaomanarivo, Lala Harivelo Raveloson ; Reynaud, Bernard ; Delatte, Hélène</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-6340836144d31893f1cebe6f99dda726065a14f17c2b6eb0c2d85af418a743533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>alleles</topic><topic>Apis mellifera</topic><topic>Biomedical and Life Sciences</topic><topic>Entomology</topic><topic>genetic variation</topic><topic>haplotypes</topic><topic>honey bees</topic><topic>Life Sciences</topic><topic>microsatellite repeats</topic><topic>mitochondrial DNA</topic><topic>Original Article</topic><topic>polymerase chain reaction</topic><topic>population</topic><topic>principal component analysis</topic><topic>restriction fragment length polymorphism</topic><topic>worker bees</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rasolofoarivao, Henriette</creatorcontrib><creatorcontrib>Clémencet, Johanna</creatorcontrib><creatorcontrib>Techer, Maéva Angélique</creatorcontrib><creatorcontrib>Ravaomanarivo, Lala Harivelo Raveloson</creatorcontrib><creatorcontrib>Reynaud, Bernard</creatorcontrib><creatorcontrib>Delatte, Hélène</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Apidologie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rasolofoarivao, Henriette</au><au>Clémencet, Johanna</au><au>Techer, Maéva Angélique</au><au>Ravaomanarivo, Lala Harivelo Raveloson</au><au>Reynaud, Bernard</au><au>Delatte, Hélène</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic diversity of the endemic honeybee: Apis mellifera unicolor (Hymenoptera: Apidae) in Madagascar</atitle><jtitle>Apidologie</jtitle><stitle>Apidologie</stitle><date>2015-11-01</date><risdate>2015</risdate><volume>46</volume><issue>6</issue><spage>735</spage><epage>747</epage><pages>735-747</pages><issn>0044-8435</issn><eissn>1297-9678</eissn><abstract>Apis mellifera unicolor is a tropical honeybee endemic of Madagascar. Comprehensive knowledge about its mitochondrial and nuclear genetic diversity and structuration was our main purpose. Samples of worker bees were collected from 867 colonies in 76 sites in Madagascar and 1 reference population in South Africa. PCR-restriction fragment length polymorphism (RFLP) and sequencing were used to reveal variability in the COI–COII mtDNA region. Seventeen microsatellite loci were used for studying the nuclear diversity. Three PCR-RFLP profiles were observed, among which 99.4 % belonged to A1 haplotype, 0.2 % to a new A haplotype, and 0.4 % to A4 haplotype. In microsatellite analysis, moderate genetic diversity values were found for Madagascar, together with low mean number of alleles ranging from 2.47 to 3.88 compared to South Africa. Bayesian clustering assignment methods and principal component analysis (PCA) separated populations into two genetic clusters which matched with geographic areas. Several hypotheses are discussed regarding to the low genetic diversity of A. m. unicolor in its native range.</abstract><cop>Paris</cop><pub>Springer Paris</pub><doi>10.1007/s13592-015-0362-1</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| source | SpringerNature Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | alleles Apis mellifera Biomedical and Life Sciences Entomology genetic variation haplotypes honey bees Life Sciences microsatellite repeats mitochondrial DNA Original Article polymerase chain reaction population principal component analysis restriction fragment length polymorphism worker bees |
| title | Genetic diversity of the endemic honeybee: Apis mellifera unicolor (Hymenoptera: Apidae) in Madagascar |
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