Serial Femtosecond Crystallography and Ultrafast Absorption Spectroscopy of the Photoswitchable Fluorescent Protein IrisFP
Reversibly photoswitchable fluorescent proteins find growing applications in cell biology, yet mechanistic details, in particular on the ultrafast photochemical time scale, remain unknown. We employed time-resolved pump–probe absorption spectroscopy on the reversibly photoswitchable fluorescent prot...
Gespeichert in:
| Veröffentlicht in: | The journal of physical chemistry letters 2016-03, Vol.7 (5), p.882-887 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 887 |
|---|---|
| container_issue | 5 |
| container_start_page | 882 |
| container_title | The journal of physical chemistry letters |
| container_volume | 7 |
| creator | Colletier, Jacques-Philippe Sliwa, Michel Gallat, François-Xavier Sugahara, Michihiro Guillon, Virginia Schirò, Giorgio Coquelle, Nicolas Woodhouse, Joyce Roux, Laure Gotthard, Guillaume Royant, Antoine Uriarte, Lucas Martinez Ruckebusch, Cyril Joti, Yasumasa Byrdin, Martin Mizohata, Eiichi Nango, Eriko Tanaka, Tomoyuki Tono, Kensuke Yabashi, Makina Adam, Virgile Cammarata, Marco Schlichting, Ilme Bourgeois, Dominique Weik, Martin |
| description | Reversibly photoswitchable fluorescent proteins find growing applications in cell biology, yet mechanistic details, in particular on the ultrafast photochemical time scale, remain unknown. We employed time-resolved pump–probe absorption spectroscopy on the reversibly photoswitchable fluorescent protein IrisFP in solution to study photoswitching from the nonfluorescent (off) to the fluorescent (on) state. Evidence is provided for the existence of several intermediate states on the pico- and microsecond time scales that are attributed to chromophore isomerization and proton transfer, respectively. Kinetic modeling favors a sequential mechanism with the existence of two excited state intermediates with lifetimes of 2 and 15 ps, the second of which controls the photoswitching quantum yield. In order to support that IrisFP is suited for time-resolved experiments aiming at a structural characterization of these ps intermediates, we used serial femtosecond crystallography at an X-ray free electron laser and solved the structure of IrisFP in its on state. Sample consumption was minimized by embedding crystals in mineral grease, in which they remain photoswitchable. Our spectroscopic and structural results pave the way for time-resolved serial femtosecond crystallography aiming at characterizing the structure of ultrafast intermediates in reversibly photoswitchable fluorescent proteins. |
| doi_str_mv | 10.1021/acs.jpclett.5b02789 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_01285611v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1770866602</sourcerecordid><originalsourceid>FETCH-LOGICAL-a445t-5241d9267e38972a004aefd10adb72c08bce9f94bb6f22e72b4aa5ca373458493</originalsourceid><addsrcrecordid>eNp9kU9rGzEQxUVpaNIkn6BQdGwPdiTtH0lHY-omYKghyVnMame7G-TVVtKmuJ--Su2GnnoaMfzeG_EeIR84W3Im-A3YuHyarMOUllXDhFT6DbngulQLyVX19p_3OXkf4xNjtWZKviPnolZ1XWh2QX7dYxjA0Q3uk49o_djSdTjEBM757wGm_kAh7x5dCtBBTHTVRB-mNPiR3k9oU_DR-ulAfUdTj3TX-2z0c0i2h8Yh3bjZB4wWx0R3wSccRnoXhrjZXZGzDlzE69O8JI-bLw_r28X229e79Wq7gLKs0qISJW-1qCUWSksBjJWAXcsZtI0UlqnGou502TR1JwRK0ZQAlYVCFmWlSl1cks9H3x6cmcKwh3AwHgZzu9qalx3jQlU15888s5-O7BT8jxljMvsh_905GNHP0XApWY6uZiKjxRG1OYEYsHv15sy8FGRyQeZUkDkVlFUfTwfmZo_tq-ZvIxm4OQJ_1H4OY87mv5a_AbFcoXk</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1770866602</pqid></control><display><type>article</type><title>Serial Femtosecond Crystallography and Ultrafast Absorption Spectroscopy of the Photoswitchable Fluorescent Protein IrisFP</title><source>MEDLINE</source><source>American Chemical Society Journals</source><creator>Colletier, Jacques-Philippe ; Sliwa, Michel ; Gallat, François-Xavier ; Sugahara, Michihiro ; Guillon, Virginia ; Schirò, Giorgio ; Coquelle, Nicolas ; Woodhouse, Joyce ; Roux, Laure ; Gotthard, Guillaume ; Royant, Antoine ; Uriarte, Lucas Martinez ; Ruckebusch, Cyril ; Joti, Yasumasa ; Byrdin, Martin ; Mizohata, Eiichi ; Nango, Eriko ; Tanaka, Tomoyuki ; Tono, Kensuke ; Yabashi, Makina ; Adam, Virgile ; Cammarata, Marco ; Schlichting, Ilme ; Bourgeois, Dominique ; Weik, Martin</creator><creatorcontrib>Colletier, Jacques-Philippe ; Sliwa, Michel ; Gallat, François-Xavier ; Sugahara, Michihiro ; Guillon, Virginia ; Schirò, Giorgio ; Coquelle, Nicolas ; Woodhouse, Joyce ; Roux, Laure ; Gotthard, Guillaume ; Royant, Antoine ; Uriarte, Lucas Martinez ; Ruckebusch, Cyril ; Joti, Yasumasa ; Byrdin, Martin ; Mizohata, Eiichi ; Nango, Eriko ; Tanaka, Tomoyuki ; Tono, Kensuke ; Yabashi, Makina ; Adam, Virgile ; Cammarata, Marco ; Schlichting, Ilme ; Bourgeois, Dominique ; Weik, Martin</creatorcontrib><description>Reversibly photoswitchable fluorescent proteins find growing applications in cell biology, yet mechanistic details, in particular on the ultrafast photochemical time scale, remain unknown. We employed time-resolved pump–probe absorption spectroscopy on the reversibly photoswitchable fluorescent protein IrisFP in solution to study photoswitching from the nonfluorescent (off) to the fluorescent (on) state. Evidence is provided for the existence of several intermediate states on the pico- and microsecond time scales that are attributed to chromophore isomerization and proton transfer, respectively. Kinetic modeling favors a sequential mechanism with the existence of two excited state intermediates with lifetimes of 2 and 15 ps, the second of which controls the photoswitching quantum yield. In order to support that IrisFP is suited for time-resolved experiments aiming at a structural characterization of these ps intermediates, we used serial femtosecond crystallography at an X-ray free electron laser and solved the structure of IrisFP in its on state. Sample consumption was minimized by embedding crystals in mineral grease, in which they remain photoswitchable. Our spectroscopic and structural results pave the way for time-resolved serial femtosecond crystallography aiming at characterizing the structure of ultrafast intermediates in reversibly photoswitchable fluorescent proteins.</description><identifier>ISSN: 1948-7185</identifier><identifier>EISSN: 1948-7185</identifier><identifier>DOI: 10.1021/acs.jpclett.5b02789</identifier><identifier>PMID: 26866390</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Chemical Sciences ; Crystallography - methods ; Luminescent Proteins - chemistry ; or physical chemistry ; Spectrum Analysis - methods ; Theoretical and</subject><ispartof>The journal of physical chemistry letters, 2016-03, Vol.7 (5), p.882-887</ispartof><rights>Copyright © 2016 American Chemical Society</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a445t-5241d9267e38972a004aefd10adb72c08bce9f94bb6f22e72b4aa5ca373458493</citedby><cites>FETCH-LOGICAL-a445t-5241d9267e38972a004aefd10adb72c08bce9f94bb6f22e72b4aa5ca373458493</cites><orcidid>0000-0002-1862-7712 ; 0000-0003-2209-7846 ; 0000-0003-1819-4686 ; 0000-0002-1919-8649 ; 0000-0002-2472-1684 ; 0000-0002-5073-8180 ; 0000-0002-4383-0368 ; 0000-0003-3013-1186 ; 0000-0001-8120-4133 ; 0000-0002-6389-8714</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jpclett.5b02789$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jpclett.5b02789$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,780,784,885,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26866390$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01285611$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Colletier, Jacques-Philippe</creatorcontrib><creatorcontrib>Sliwa, Michel</creatorcontrib><creatorcontrib>Gallat, François-Xavier</creatorcontrib><creatorcontrib>Sugahara, Michihiro</creatorcontrib><creatorcontrib>Guillon, Virginia</creatorcontrib><creatorcontrib>Schirò, Giorgio</creatorcontrib><creatorcontrib>Coquelle, Nicolas</creatorcontrib><creatorcontrib>Woodhouse, Joyce</creatorcontrib><creatorcontrib>Roux, Laure</creatorcontrib><creatorcontrib>Gotthard, Guillaume</creatorcontrib><creatorcontrib>Royant, Antoine</creatorcontrib><creatorcontrib>Uriarte, Lucas Martinez</creatorcontrib><creatorcontrib>Ruckebusch, Cyril</creatorcontrib><creatorcontrib>Joti, Yasumasa</creatorcontrib><creatorcontrib>Byrdin, Martin</creatorcontrib><creatorcontrib>Mizohata, Eiichi</creatorcontrib><creatorcontrib>Nango, Eriko</creatorcontrib><creatorcontrib>Tanaka, Tomoyuki</creatorcontrib><creatorcontrib>Tono, Kensuke</creatorcontrib><creatorcontrib>Yabashi, Makina</creatorcontrib><creatorcontrib>Adam, Virgile</creatorcontrib><creatorcontrib>Cammarata, Marco</creatorcontrib><creatorcontrib>Schlichting, Ilme</creatorcontrib><creatorcontrib>Bourgeois, Dominique</creatorcontrib><creatorcontrib>Weik, Martin</creatorcontrib><title>Serial Femtosecond Crystallography and Ultrafast Absorption Spectroscopy of the Photoswitchable Fluorescent Protein IrisFP</title><title>The journal of physical chemistry letters</title><addtitle>J. Phys. Chem. Lett</addtitle><description>Reversibly photoswitchable fluorescent proteins find growing applications in cell biology, yet mechanistic details, in particular on the ultrafast photochemical time scale, remain unknown. We employed time-resolved pump–probe absorption spectroscopy on the reversibly photoswitchable fluorescent protein IrisFP in solution to study photoswitching from the nonfluorescent (off) to the fluorescent (on) state. Evidence is provided for the existence of several intermediate states on the pico- and microsecond time scales that are attributed to chromophore isomerization and proton transfer, respectively. Kinetic modeling favors a sequential mechanism with the existence of two excited state intermediates with lifetimes of 2 and 15 ps, the second of which controls the photoswitching quantum yield. In order to support that IrisFP is suited for time-resolved experiments aiming at a structural characterization of these ps intermediates, we used serial femtosecond crystallography at an X-ray free electron laser and solved the structure of IrisFP in its on state. Sample consumption was minimized by embedding crystals in mineral grease, in which they remain photoswitchable. Our spectroscopic and structural results pave the way for time-resolved serial femtosecond crystallography aiming at characterizing the structure of ultrafast intermediates in reversibly photoswitchable fluorescent proteins.</description><subject>Chemical Sciences</subject><subject>Crystallography - methods</subject><subject>Luminescent Proteins - chemistry</subject><subject>or physical chemistry</subject><subject>Spectrum Analysis - methods</subject><subject>Theoretical and</subject><issn>1948-7185</issn><issn>1948-7185</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9rGzEQxUVpaNIkn6BQdGwPdiTtH0lHY-omYKghyVnMame7G-TVVtKmuJ--Su2GnnoaMfzeG_EeIR84W3Im-A3YuHyarMOUllXDhFT6DbngulQLyVX19p_3OXkf4xNjtWZKviPnolZ1XWh2QX7dYxjA0Q3uk49o_djSdTjEBM757wGm_kAh7x5dCtBBTHTVRB-mNPiR3k9oU_DR-ulAfUdTj3TX-2z0c0i2h8Yh3bjZB4wWx0R3wSccRnoXhrjZXZGzDlzE69O8JI-bLw_r28X229e79Wq7gLKs0qISJW-1qCUWSksBjJWAXcsZtI0UlqnGou502TR1JwRK0ZQAlYVCFmWlSl1cks9H3x6cmcKwh3AwHgZzu9qalx3jQlU15888s5-O7BT8jxljMvsh_905GNHP0XApWY6uZiKjxRG1OYEYsHv15sy8FGRyQeZUkDkVlFUfTwfmZo_tq-ZvIxm4OQJ_1H4OY87mv5a_AbFcoXk</recordid><startdate>20160303</startdate><enddate>20160303</enddate><creator>Colletier, Jacques-Philippe</creator><creator>Sliwa, Michel</creator><creator>Gallat, François-Xavier</creator><creator>Sugahara, Michihiro</creator><creator>Guillon, Virginia</creator><creator>Schirò, Giorgio</creator><creator>Coquelle, Nicolas</creator><creator>Woodhouse, Joyce</creator><creator>Roux, Laure</creator><creator>Gotthard, Guillaume</creator><creator>Royant, Antoine</creator><creator>Uriarte, Lucas Martinez</creator><creator>Ruckebusch, Cyril</creator><creator>Joti, Yasumasa</creator><creator>Byrdin, Martin</creator><creator>Mizohata, Eiichi</creator><creator>Nango, Eriko</creator><creator>Tanaka, Tomoyuki</creator><creator>Tono, Kensuke</creator><creator>Yabashi, Makina</creator><creator>Adam, Virgile</creator><creator>Cammarata, Marco</creator><creator>Schlichting, Ilme</creator><creator>Bourgeois, Dominique</creator><creator>Weik, Martin</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-1862-7712</orcidid><orcidid>https://orcid.org/0000-0003-2209-7846</orcidid><orcidid>https://orcid.org/0000-0003-1819-4686</orcidid><orcidid>https://orcid.org/0000-0002-1919-8649</orcidid><orcidid>https://orcid.org/0000-0002-2472-1684</orcidid><orcidid>https://orcid.org/0000-0002-5073-8180</orcidid><orcidid>https://orcid.org/0000-0002-4383-0368</orcidid><orcidid>https://orcid.org/0000-0003-3013-1186</orcidid><orcidid>https://orcid.org/0000-0001-8120-4133</orcidid><orcidid>https://orcid.org/0000-0002-6389-8714</orcidid></search><sort><creationdate>20160303</creationdate><title>Serial Femtosecond Crystallography and Ultrafast Absorption Spectroscopy of the Photoswitchable Fluorescent Protein IrisFP</title><author>Colletier, Jacques-Philippe ; Sliwa, Michel ; Gallat, François-Xavier ; Sugahara, Michihiro ; Guillon, Virginia ; Schirò, Giorgio ; Coquelle, Nicolas ; Woodhouse, Joyce ; Roux, Laure ; Gotthard, Guillaume ; Royant, Antoine ; Uriarte, Lucas Martinez ; Ruckebusch, Cyril ; Joti, Yasumasa ; Byrdin, Martin ; Mizohata, Eiichi ; Nango, Eriko ; Tanaka, Tomoyuki ; Tono, Kensuke ; Yabashi, Makina ; Adam, Virgile ; Cammarata, Marco ; Schlichting, Ilme ; Bourgeois, Dominique ; Weik, Martin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a445t-5241d9267e38972a004aefd10adb72c08bce9f94bb6f22e72b4aa5ca373458493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Chemical Sciences</topic><topic>Crystallography - methods</topic><topic>Luminescent Proteins - chemistry</topic><topic>or physical chemistry</topic><topic>Spectrum Analysis - methods</topic><topic>Theoretical and</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Colletier, Jacques-Philippe</creatorcontrib><creatorcontrib>Sliwa, Michel</creatorcontrib><creatorcontrib>Gallat, François-Xavier</creatorcontrib><creatorcontrib>Sugahara, Michihiro</creatorcontrib><creatorcontrib>Guillon, Virginia</creatorcontrib><creatorcontrib>Schirò, Giorgio</creatorcontrib><creatorcontrib>Coquelle, Nicolas</creatorcontrib><creatorcontrib>Woodhouse, Joyce</creatorcontrib><creatorcontrib>Roux, Laure</creatorcontrib><creatorcontrib>Gotthard, Guillaume</creatorcontrib><creatorcontrib>Royant, Antoine</creatorcontrib><creatorcontrib>Uriarte, Lucas Martinez</creatorcontrib><creatorcontrib>Ruckebusch, Cyril</creatorcontrib><creatorcontrib>Joti, Yasumasa</creatorcontrib><creatorcontrib>Byrdin, Martin</creatorcontrib><creatorcontrib>Mizohata, Eiichi</creatorcontrib><creatorcontrib>Nango, Eriko</creatorcontrib><creatorcontrib>Tanaka, Tomoyuki</creatorcontrib><creatorcontrib>Tono, Kensuke</creatorcontrib><creatorcontrib>Yabashi, Makina</creatorcontrib><creatorcontrib>Adam, Virgile</creatorcontrib><creatorcontrib>Cammarata, Marco</creatorcontrib><creatorcontrib>Schlichting, Ilme</creatorcontrib><creatorcontrib>Bourgeois, Dominique</creatorcontrib><creatorcontrib>Weik, Martin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>The journal of physical chemistry letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Colletier, Jacques-Philippe</au><au>Sliwa, Michel</au><au>Gallat, François-Xavier</au><au>Sugahara, Michihiro</au><au>Guillon, Virginia</au><au>Schirò, Giorgio</au><au>Coquelle, Nicolas</au><au>Woodhouse, Joyce</au><au>Roux, Laure</au><au>Gotthard, Guillaume</au><au>Royant, Antoine</au><au>Uriarte, Lucas Martinez</au><au>Ruckebusch, Cyril</au><au>Joti, Yasumasa</au><au>Byrdin, Martin</au><au>Mizohata, Eiichi</au><au>Nango, Eriko</au><au>Tanaka, Tomoyuki</au><au>Tono, Kensuke</au><au>Yabashi, Makina</au><au>Adam, Virgile</au><au>Cammarata, Marco</au><au>Schlichting, Ilme</au><au>Bourgeois, Dominique</au><au>Weik, Martin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serial Femtosecond Crystallography and Ultrafast Absorption Spectroscopy of the Photoswitchable Fluorescent Protein IrisFP</atitle><jtitle>The journal of physical chemistry letters</jtitle><addtitle>J. Phys. Chem. Lett</addtitle><date>2016-03-03</date><risdate>2016</risdate><volume>7</volume><issue>5</issue><spage>882</spage><epage>887</epage><pages>882-887</pages><issn>1948-7185</issn><eissn>1948-7185</eissn><abstract>Reversibly photoswitchable fluorescent proteins find growing applications in cell biology, yet mechanistic details, in particular on the ultrafast photochemical time scale, remain unknown. We employed time-resolved pump–probe absorption spectroscopy on the reversibly photoswitchable fluorescent protein IrisFP in solution to study photoswitching from the nonfluorescent (off) to the fluorescent (on) state. Evidence is provided for the existence of several intermediate states on the pico- and microsecond time scales that are attributed to chromophore isomerization and proton transfer, respectively. Kinetic modeling favors a sequential mechanism with the existence of two excited state intermediates with lifetimes of 2 and 15 ps, the second of which controls the photoswitching quantum yield. In order to support that IrisFP is suited for time-resolved experiments aiming at a structural characterization of these ps intermediates, we used serial femtosecond crystallography at an X-ray free electron laser and solved the structure of IrisFP in its on state. Sample consumption was minimized by embedding crystals in mineral grease, in which they remain photoswitchable. Our spectroscopic and structural results pave the way for time-resolved serial femtosecond crystallography aiming at characterizing the structure of ultrafast intermediates in reversibly photoswitchable fluorescent proteins.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>26866390</pmid><doi>10.1021/acs.jpclett.5b02789</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-1862-7712</orcidid><orcidid>https://orcid.org/0000-0003-2209-7846</orcidid><orcidid>https://orcid.org/0000-0003-1819-4686</orcidid><orcidid>https://orcid.org/0000-0002-1919-8649</orcidid><orcidid>https://orcid.org/0000-0002-2472-1684</orcidid><orcidid>https://orcid.org/0000-0002-5073-8180</orcidid><orcidid>https://orcid.org/0000-0002-4383-0368</orcidid><orcidid>https://orcid.org/0000-0003-3013-1186</orcidid><orcidid>https://orcid.org/0000-0001-8120-4133</orcidid><orcidid>https://orcid.org/0000-0002-6389-8714</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1948-7185 |
| ispartof | The journal of physical chemistry letters, 2016-03, Vol.7 (5), p.882-887 |
| issn | 1948-7185 1948-7185 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_01285611v1 |
| source | MEDLINE; American Chemical Society Journals |
| subjects | Chemical Sciences Crystallography - methods Luminescent Proteins - chemistry or physical chemistry Spectrum Analysis - methods Theoretical and |
| title | Serial Femtosecond Crystallography and Ultrafast Absorption Spectroscopy of the Photoswitchable Fluorescent Protein IrisFP |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T12%3A34%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Serial%20Femtosecond%20Crystallography%20and%20Ultrafast%20Absorption%20Spectroscopy%20of%20the%20Photoswitchable%20Fluorescent%20Protein%20IrisFP&rft.jtitle=The%20journal%20of%20physical%20chemistry%20letters&rft.au=Colletier,%20Jacques-Philippe&rft.date=2016-03-03&rft.volume=7&rft.issue=5&rft.spage=882&rft.epage=887&rft.pages=882-887&rft.issn=1948-7185&rft.eissn=1948-7185&rft_id=info:doi/10.1021/acs.jpclett.5b02789&rft_dat=%3Cproquest_hal_p%3E1770866602%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1770866602&rft_id=info:pmid/26866390&rfr_iscdi=true |