Proteomic Approach to Identify Nuclear Proteins in Wheat Grain
The nuclear proteome of the grain of the two cultivated wheat species Triticum aestivum (hexaploid wheat; genomes A, B, and D) and T. monococcum (diploid wheat; genome A) was analyzed in two early stages of development using shotgun-based proteomics. A procedure was optimized to purify nuclei, and a...
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| Veröffentlicht in: | Journal of proteome research 2015-10, Vol.14 (10), p.4432-4439 |
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| container_title | Journal of proteome research |
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| creator | Bancel, Emmanuelle Bonnot, Titouan Davanture, Marlène Branlard, Gérard Zivy, Michel Martre, Pierre |
| description | The nuclear proteome of the grain of the two cultivated wheat species Triticum aestivum (hexaploid wheat; genomes A, B, and D) and T. monococcum (diploid wheat; genome A) was analyzed in two early stages of development using shotgun-based proteomics. A procedure was optimized to purify nuclei, and an improved protein sample preparation was developed to efficiently remove nonprotein substances (starch and nucleic acids). A total of 797 proteins corresponding to 528 unique proteins were identified, 36% of which were classified in functional groups related to DNA and RNA metabolism. A large number (107 proteins) of unknown functions and hypothetical proteins were also found. Some identified proteins may be multifunctional and may present multiple localizations. On the basis of the MS/MS analysis, 368 proteins were present in the two species, and in two stages of development, some qualitative differences between species and stages of development were also found. All of these data illustrate the dynamic function of the grain nucleus in the early stages of development. |
| doi_str_mv | 10.1021/acs.jproteome.5b00446 |
| format | Article |
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A procedure was optimized to purify nuclei, and an improved protein sample preparation was developed to efficiently remove nonprotein substances (starch and nucleic acids). A total of 797 proteins corresponding to 528 unique proteins were identified, 36% of which were classified in functional groups related to DNA and RNA metabolism. A large number (107 proteins) of unknown functions and hypothetical proteins were also found. Some identified proteins may be multifunctional and may present multiple localizations. On the basis of the MS/MS analysis, 368 proteins were present in the two species, and in two stages of development, some qualitative differences between species and stages of development were also found. All of these data illustrate the dynamic function of the grain nucleus in the early stages of development.</description><identifier>ISSN: 1535-3893</identifier><identifier>EISSN: 1535-3907</identifier><identifier>DOI: 10.1021/acs.jproteome.5b00446</identifier><identifier>PMID: 26228564</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Cell Nucleus - chemistry ; Cell Nucleus - genetics ; Cell Nucleus - metabolism ; Chromatography, Liquid ; Edible Grain - chemistry ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Plant ; Genome, Plant ; Life Sciences ; Mass Spectrometry ; Metabolic Networks and Pathways - genetics ; Molecular Sequence Annotation ; Nuclear Proteins - isolation & purification ; Plant Proteins - isolation & purification ; Proteome - genetics ; Proteome - isolation & purification ; Proteome - metabolism ; Proteomics ; Species Specificity ; Triticum - classification ; Triticum - genetics ; Triticum - growth & development ; Triticum - metabolism ; Vegetal Biology</subject><ispartof>Journal of proteome research, 2015-10, Vol.14 (10), p.4432-4439</ispartof><rights>Copyright © 2015 American Chemical Society</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a451t-625c285402bf72a58e9608f974b5f70c4cafe0c74ab39dc04ca3d5a6aa3b9a7a3</citedby><cites>FETCH-LOGICAL-a451t-625c285402bf72a58e9608f974b5f70c4cafe0c74ab39dc04ca3d5a6aa3b9a7a3</cites><orcidid>0000-0001-9560-535X ; 0000-0001-5324-0583 ; 0009-0008-0750-8823</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jproteome.5b00446$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jproteome.5b00446$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,776,780,881,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26228564$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01240237$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Bancel, Emmanuelle</creatorcontrib><creatorcontrib>Bonnot, Titouan</creatorcontrib><creatorcontrib>Davanture, Marlène</creatorcontrib><creatorcontrib>Branlard, Gérard</creatorcontrib><creatorcontrib>Zivy, Michel</creatorcontrib><creatorcontrib>Martre, Pierre</creatorcontrib><title>Proteomic Approach to Identify Nuclear Proteins in Wheat Grain</title><title>Journal of proteome research</title><addtitle>J. Proteome Res</addtitle><description>The nuclear proteome of the grain of the two cultivated wheat species Triticum aestivum (hexaploid wheat; genomes A, B, and D) and T. monococcum (diploid wheat; genome A) was analyzed in two early stages of development using shotgun-based proteomics. A procedure was optimized to purify nuclei, and an improved protein sample preparation was developed to efficiently remove nonprotein substances (starch and nucleic acids). A total of 797 proteins corresponding to 528 unique proteins were identified, 36% of which were classified in functional groups related to DNA and RNA metabolism. A large number (107 proteins) of unknown functions and hypothetical proteins were also found. Some identified proteins may be multifunctional and may present multiple localizations. On the basis of the MS/MS analysis, 368 proteins were present in the two species, and in two stages of development, some qualitative differences between species and stages of development were also found. All of these data illustrate the dynamic function of the grain nucleus in the early stages of development.</description><subject>Cell Nucleus - chemistry</subject><subject>Cell Nucleus - genetics</subject><subject>Cell Nucleus - metabolism</subject><subject>Chromatography, Liquid</subject><subject>Edible Grain - chemistry</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genome, Plant</subject><subject>Life Sciences</subject><subject>Mass Spectrometry</subject><subject>Metabolic Networks and Pathways - genetics</subject><subject>Molecular Sequence Annotation</subject><subject>Nuclear Proteins - isolation & purification</subject><subject>Plant Proteins - isolation & purification</subject><subject>Proteome - genetics</subject><subject>Proteome - isolation & purification</subject><subject>Proteome - metabolism</subject><subject>Proteomics</subject><subject>Species Specificity</subject><subject>Triticum - classification</subject><subject>Triticum - genetics</subject><subject>Triticum - growth & development</subject><subject>Triticum - metabolism</subject><subject>Vegetal Biology</subject><issn>1535-3893</issn><issn>1535-3907</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1PwkAURSdGI4j-BM0sdVGcz067MSFEgYSoC43Lyet0GkpKizOtCf_egSJbV_ORc8_LuwjdUjKmhNFHMH683rqmtc3GjmVGiBDxGRpSyWXEU6LO_-5Jygfoyvs1IVQqwi_RgMWMJTIWQ_T03itKgyfboAOzwm2DF7mt27LY4dfOVBYcPmBl7XFZ46-VhRbPHJT1NboooPL25niO0OfL88d0Hi3fZovpZBmBkLSNYiZNGCgIywrFQCY2jUlSpEpkslDECAOFJUYJyHiaGxLePJcQA_AsBQV8hB567woqvXXlBtxON1Dq-WSp93-EsmDn6ocG9r5nwzrfnfWt3pTe2KqC2jad11TRJCWpCvgIyR41rvHe2eLkpkTva9ahZn2qWR9rDrm744gu29j8lPrrNQC0Bw75pnN1aOcf6S-s-oxj</recordid><startdate>20151002</startdate><enddate>20151002</enddate><creator>Bancel, Emmanuelle</creator><creator>Bonnot, Titouan</creator><creator>Davanture, Marlène</creator><creator>Branlard, Gérard</creator><creator>Zivy, Michel</creator><creator>Martre, Pierre</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-9560-535X</orcidid><orcidid>https://orcid.org/0000-0001-5324-0583</orcidid><orcidid>https://orcid.org/0009-0008-0750-8823</orcidid></search><sort><creationdate>20151002</creationdate><title>Proteomic Approach to Identify Nuclear Proteins in Wheat Grain</title><author>Bancel, Emmanuelle ; Bonnot, Titouan ; Davanture, Marlène ; Branlard, Gérard ; Zivy, Michel ; Martre, Pierre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a451t-625c285402bf72a58e9608f974b5f70c4cafe0c74ab39dc04ca3d5a6aa3b9a7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Cell Nucleus - chemistry</topic><topic>Cell Nucleus - genetics</topic><topic>Cell Nucleus - metabolism</topic><topic>Chromatography, Liquid</topic><topic>Edible Grain - chemistry</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genome, Plant</topic><topic>Life Sciences</topic><topic>Mass Spectrometry</topic><topic>Metabolic Networks and Pathways - genetics</topic><topic>Molecular Sequence Annotation</topic><topic>Nuclear Proteins - isolation & purification</topic><topic>Plant Proteins - isolation & purification</topic><topic>Proteome - genetics</topic><topic>Proteome - isolation & purification</topic><topic>Proteome - metabolism</topic><topic>Proteomics</topic><topic>Species Specificity</topic><topic>Triticum - classification</topic><topic>Triticum - genetics</topic><topic>Triticum - growth & development</topic><topic>Triticum - metabolism</topic><topic>Vegetal Biology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bancel, Emmanuelle</creatorcontrib><creatorcontrib>Bonnot, Titouan</creatorcontrib><creatorcontrib>Davanture, Marlène</creatorcontrib><creatorcontrib>Branlard, Gérard</creatorcontrib><creatorcontrib>Zivy, Michel</creatorcontrib><creatorcontrib>Martre, Pierre</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of proteome research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bancel, Emmanuelle</au><au>Bonnot, Titouan</au><au>Davanture, Marlène</au><au>Branlard, Gérard</au><au>Zivy, Michel</au><au>Martre, Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomic Approach to Identify Nuclear Proteins in Wheat Grain</atitle><jtitle>Journal of proteome research</jtitle><addtitle>J. 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| subjects | Cell Nucleus - chemistry Cell Nucleus - genetics Cell Nucleus - metabolism Chromatography, Liquid Edible Grain - chemistry Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genome, Plant Life Sciences Mass Spectrometry Metabolic Networks and Pathways - genetics Molecular Sequence Annotation Nuclear Proteins - isolation & purification Plant Proteins - isolation & purification Proteome - genetics Proteome - isolation & purification Proteome - metabolism Proteomics Species Specificity Triticum - classification Triticum - genetics Triticum - growth & development Triticum - metabolism Vegetal Biology |
| title | Proteomic Approach to Identify Nuclear Proteins in Wheat Grain |
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