In vitro susceptibility of Varroa destructor and Apis mellifera to native strains of Bacillus thuringiensis
Fifty-four Bacillus-like strains were isolated from dead Varroa destructor collected in 24 colonies of bees from seven apiaries. The total proteins of 22 isolates were evaluated against adult female V. destructor (100 μg/mL), but only nine caused over 80 % mortality. The LC₅₀ of EA26.1, EA3 and EA11...
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| Veröffentlicht in: | Apidologie 2014, Vol.45 (6), p.707-718 |
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| creator | Alquisira-Ramírez, Eva Vianey Paredes-Gonzalez, José Roberto Hernández-Velázquez, Víctor Manuel Ramírez-Trujillo, José Augusto Peña-Chora, Guadalupe |
| description | Fifty-four Bacillus-like strains were isolated from dead Varroa destructor collected in 24 colonies of bees from seven apiaries. The total proteins of 22 isolates were evaluated against adult female V. destructor (100 μg/mL), but only nine caused over 80 % mortality. The LC₅₀ of EA26.1, EA3 and EA11.3 isolates were calculated. The most virulent isolate was EA26.1, as 96.7 % of the mites were killed 36 h after treatment, and the LC₅₀ was 1.50 μg/mL. EA3 produced 93.3 % mite mortality after 48 h treatment, with an LC₅₀ of 7.1 μg/mL. Finally, EA11.3 produced 93.3 % mite mortality after 60 h treatment, with an LC₅₀ of 22.8 μg/mL. Additionally, the EA3 and EA26.1 isolates were used to determine mortality on adults and larvae of worker bees of A. mellifera. When the 16S rRNA gene was amplified and sequenced, the three strains were found to belong to the species B. thuringiensis. The results showed that honey bee adults and larvae are not negatively affected by identified B. thuringiensis isolates, indicating that they could be successfully used for the biological control of V. destructor without adverse effects on adults and larvae of A. mellifera, and reduce the negative impact of this mite on colonies. |
| doi_str_mv | 10.1007/s13592-014-0288-z |
| format | Article |
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The total proteins of 22 isolates were evaluated against adult female V. destructor (100 μg/mL), but only nine caused over 80 % mortality. The LC₅₀ of EA26.1, EA3 and EA11.3 isolates were calculated. The most virulent isolate was EA26.1, as 96.7 % of the mites were killed 36 h after treatment, and the LC₅₀ was 1.50 μg/mL. EA3 produced 93.3 % mite mortality after 48 h treatment, with an LC₅₀ of 7.1 μg/mL. Finally, EA11.3 produced 93.3 % mite mortality after 60 h treatment, with an LC₅₀ of 22.8 μg/mL. Additionally, the EA3 and EA26.1 isolates were used to determine mortality on adults and larvae of worker bees of A. mellifera. When the 16S rRNA gene was amplified and sequenced, the three strains were found to belong to the species B. thuringiensis. The results showed that honey bee adults and larvae are not negatively affected by identified B. thuringiensis isolates, indicating that they could be successfully used for the biological control of V. destructor without adverse effects on adults and larvae of A. mellifera, and reduce the negative impact of this mite on colonies.</description><identifier>ISSN: 0044-8435</identifier><identifier>EISSN: 1297-9678</identifier><identifier>DOI: 10.1007/s13592-014-0288-z</identifier><identifier>CODEN: APDGB5</identifier><language>eng</language><publisher>Paris: Springer-Verlag</publisher><subject>adults ; adverse effects ; Agricultural sciences ; Animal biology ; Animal production studies ; Animal productions ; apiaries ; Apiculture ; Apis mellifera ; Bacillus thuringiensis ; Biodiversity ; Biological and medical sciences ; biological control ; Biomedical and Life Sciences ; Ecology, environment ; Entomology ; Fundamental and applied biological sciences. Psychology ; genes ; honey bees ; insect larvae ; Insecta ; Invertebrate Zoology ; Invertebrates ; lethal concentration 50 ; Life Sciences ; mites ; mortality ; Original Article ; proteins ; ribosomal RNA ; Terrestrial animal productions ; Varroa destructor ; worker bees</subject><ispartof>Apidologie, 2014, Vol.45 (6), p.707-718</ispartof><rights>INRA, DIB and Springer-Verlag France 2014</rights><rights>2015 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-63e5bc4d98595c849ce79fdceb6713ff7686f18530b73de0efc3b9472f52a1933</citedby><cites>FETCH-LOGICAL-c419t-63e5bc4d98595c849ce79fdceb6713ff7686f18530b73de0efc3b9472f52a1933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13592-014-0288-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13592-014-0288-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28938199$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01234771$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Alquisira-Ramírez, Eva Vianey</creatorcontrib><creatorcontrib>Paredes-Gonzalez, José Roberto</creatorcontrib><creatorcontrib>Hernández-Velázquez, Víctor Manuel</creatorcontrib><creatorcontrib>Ramírez-Trujillo, José Augusto</creatorcontrib><creatorcontrib>Peña-Chora, Guadalupe</creatorcontrib><title>In vitro susceptibility of Varroa destructor and Apis mellifera to native strains of Bacillus thuringiensis</title><title>Apidologie</title><addtitle>Apidologie</addtitle><description>Fifty-four Bacillus-like strains were isolated from dead Varroa destructor collected in 24 colonies of bees from seven apiaries. The total proteins of 22 isolates were evaluated against adult female V. destructor (100 μg/mL), but only nine caused over 80 % mortality. The LC₅₀ of EA26.1, EA3 and EA11.3 isolates were calculated. The most virulent isolate was EA26.1, as 96.7 % of the mites were killed 36 h after treatment, and the LC₅₀ was 1.50 μg/mL. EA3 produced 93.3 % mite mortality after 48 h treatment, with an LC₅₀ of 7.1 μg/mL. Finally, EA11.3 produced 93.3 % mite mortality after 60 h treatment, with an LC₅₀ of 22.8 μg/mL. Additionally, the EA3 and EA26.1 isolates were used to determine mortality on adults and larvae of worker bees of A. mellifera. When the 16S rRNA gene was amplified and sequenced, the three strains were found to belong to the species B. thuringiensis. The results showed that honey bee adults and larvae are not negatively affected by identified B. thuringiensis isolates, indicating that they could be successfully used for the biological control of V. destructor without adverse effects on adults and larvae of A. mellifera, and reduce the negative impact of this mite on colonies.</description><subject>adults</subject><subject>adverse effects</subject><subject>Agricultural sciences</subject><subject>Animal biology</subject><subject>Animal production studies</subject><subject>Animal productions</subject><subject>apiaries</subject><subject>Apiculture</subject><subject>Apis mellifera</subject><subject>Bacillus thuringiensis</subject><subject>Biodiversity</subject><subject>Biological and medical sciences</subject><subject>biological control</subject><subject>Biomedical and Life Sciences</subject><subject>Ecology, environment</subject><subject>Entomology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>honey bees</subject><subject>insect larvae</subject><subject>Insecta</subject><subject>Invertebrate Zoology</subject><subject>Invertebrates</subject><subject>lethal concentration 50</subject><subject>Life Sciences</subject><subject>mites</subject><subject>mortality</subject><subject>Original Article</subject><subject>proteins</subject><subject>ribosomal RNA</subject><subject>Terrestrial animal productions</subject><subject>Varroa destructor</subject><subject>worker bees</subject><issn>0044-8435</issn><issn>1297-9678</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LHTEUhoMo9Kr9AV2ZTRcuxuZzkiyvolW40IXabchkEo0dJ0OSuaC_3gxTXHo2Bw7P88J5AfiB0QVGSPzKmHJFGoRZg4iUzfsB2GCiRKNaIQ_BBiHGGsko_waOc35BCBPJ2Qb8uxvhPpQUYZ6zdVMJXRhCeYPRw78mpWhg73JJsy0xQTP2cDuFDF_dMATvkoElwtGUsHewUiaMeTEvjQ3DMGdYnucUxqfgxhzyKTjyZsju-_99Ah5vrh-ubpvdn993V9tdYxlWpWmp451lvZJccSuZsk4o31vXtQJT70UrW48lp6gTtHfIeUs7xQTxnBisKD0B52vusxn0lMKrSW86mqBvtzu93OrzlAmB97iyeGVtijkn5z8FjPTSrF6brQ7TS7P6vTo_V2cy2ZrBJzPakD9FIhWVWKnKkZXL01KCS_olzmmsr38ZfrZK3kRtnlINfrwnCHNUh6uW0A8JvJPf</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>Alquisira-Ramírez, Eva Vianey</creator><creator>Paredes-Gonzalez, José Roberto</creator><creator>Hernández-Velázquez, Víctor Manuel</creator><creator>Ramírez-Trujillo, José Augusto</creator><creator>Peña-Chora, Guadalupe</creator><general>Springer-Verlag</general><general>Springer Paris</general><general>EDP Sciences</general><general>Springer Verlag</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>1XC</scope><scope>VOOES</scope></search><sort><creationdate>2014</creationdate><title>In vitro susceptibility of Varroa destructor and Apis mellifera to native strains of Bacillus thuringiensis</title><author>Alquisira-Ramírez, Eva Vianey ; Paredes-Gonzalez, José Roberto ; Hernández-Velázquez, Víctor Manuel ; Ramírez-Trujillo, José Augusto ; Peña-Chora, Guadalupe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-63e5bc4d98595c849ce79fdceb6713ff7686f18530b73de0efc3b9472f52a1933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>adults</topic><topic>adverse effects</topic><topic>Agricultural sciences</topic><topic>Animal biology</topic><topic>Animal production studies</topic><topic>Animal productions</topic><topic>apiaries</topic><topic>Apiculture</topic><topic>Apis mellifera</topic><topic>Bacillus thuringiensis</topic><topic>Biodiversity</topic><topic>Biological and medical sciences</topic><topic>biological control</topic><topic>Biomedical and Life Sciences</topic><topic>Ecology, environment</topic><topic>Entomology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>honey bees</topic><topic>insect larvae</topic><topic>Insecta</topic><topic>Invertebrate Zoology</topic><topic>Invertebrates</topic><topic>lethal concentration 50</topic><topic>Life Sciences</topic><topic>mites</topic><topic>mortality</topic><topic>Original Article</topic><topic>proteins</topic><topic>ribosomal RNA</topic><topic>Terrestrial animal productions</topic><topic>Varroa destructor</topic><topic>worker bees</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alquisira-Ramírez, Eva Vianey</creatorcontrib><creatorcontrib>Paredes-Gonzalez, José Roberto</creatorcontrib><creatorcontrib>Hernández-Velázquez, Víctor Manuel</creatorcontrib><creatorcontrib>Ramírez-Trujillo, José Augusto</creatorcontrib><creatorcontrib>Peña-Chora, Guadalupe</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Apidologie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alquisira-Ramírez, Eva Vianey</au><au>Paredes-Gonzalez, José Roberto</au><au>Hernández-Velázquez, Víctor Manuel</au><au>Ramírez-Trujillo, José Augusto</au><au>Peña-Chora, Guadalupe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro susceptibility of Varroa destructor and Apis mellifera to native strains of Bacillus thuringiensis</atitle><jtitle>Apidologie</jtitle><stitle>Apidologie</stitle><date>2014</date><risdate>2014</risdate><volume>45</volume><issue>6</issue><spage>707</spage><epage>718</epage><pages>707-718</pages><issn>0044-8435</issn><eissn>1297-9678</eissn><coden>APDGB5</coden><abstract>Fifty-four Bacillus-like strains were isolated from dead Varroa destructor collected in 24 colonies of bees from seven apiaries. The total proteins of 22 isolates were evaluated against adult female V. destructor (100 μg/mL), but only nine caused over 80 % mortality. The LC₅₀ of EA26.1, EA3 and EA11.3 isolates were calculated. The most virulent isolate was EA26.1, as 96.7 % of the mites were killed 36 h after treatment, and the LC₅₀ was 1.50 μg/mL. EA3 produced 93.3 % mite mortality after 48 h treatment, with an LC₅₀ of 7.1 μg/mL. Finally, EA11.3 produced 93.3 % mite mortality after 60 h treatment, with an LC₅₀ of 22.8 μg/mL. Additionally, the EA3 and EA26.1 isolates were used to determine mortality on adults and larvae of worker bees of A. mellifera. When the 16S rRNA gene was amplified and sequenced, the three strains were found to belong to the species B. thuringiensis. The results showed that honey bee adults and larvae are not negatively affected by identified B. thuringiensis isolates, indicating that they could be successfully used for the biological control of V. destructor without adverse effects on adults and larvae of A. mellifera, and reduce the negative impact of this mite on colonies.</abstract><cop>Paris</cop><pub>Springer-Verlag</pub><doi>10.1007/s13592-014-0288-z</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | adults adverse effects Agricultural sciences Animal biology Animal production studies Animal productions apiaries Apiculture Apis mellifera Bacillus thuringiensis Biodiversity Biological and medical sciences biological control Biomedical and Life Sciences Ecology, environment Entomology Fundamental and applied biological sciences. Psychology genes honey bees insect larvae Insecta Invertebrate Zoology Invertebrates lethal concentration 50 Life Sciences mites mortality Original Article proteins ribosomal RNA Terrestrial animal productions Varroa destructor worker bees |
| title | In vitro susceptibility of Varroa destructor and Apis mellifera to native strains of Bacillus thuringiensis |
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