Swa2p-dependent clathrin dynamics is critical for Flo11p processing and ‘Mat’ formation in the yeast Saccharomyces cerevisiae
The yeast Saccharomyces cerevisiae is able to form complex multicellular structures called mats on low-density agar Petri plates. Mat formation strictly depends on Flo11p, a cell surface mannoprotein that mediates the adhesion of yeast cells to the agar surface. Here, we show that Swa2p, an auxilin...
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| Veröffentlicht in: | FEBS letters 2010-03, Vol.584 (6), p.1149-1155 |
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| description | The yeast Saccharomyces cerevisiae is able to form complex multicellular structures called mats on low-density agar Petri plates. Mat formation strictly depends on Flo11p, a cell surface mannoprotein that mediates the adhesion of yeast cells to the agar surface. Here, we show that Swa2p, an auxilin ortholog required for clathrin-coated vesicle uncoating, is strictly required for biofilm formation. We show that the maturation and cellular levels of Flo11p are affected in Δswa2 cells, yet without compromising invasive growth. Both the TPR and J-domains of Swa2p, but not its clathrin-binding and ubiquitin-association motifs, are required for its function in Flo11p processing. |
| doi_str_mv | 10.1016/j.febslet.2010.02.008 |
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Mat formation strictly depends on Flo11p, a cell surface mannoprotein that mediates the adhesion of yeast cells to the agar surface. Here, we show that Swa2p, an auxilin ortholog required for clathrin-coated vesicle uncoating, is strictly required for biofilm formation. We show that the maturation and cellular levels of Flo11p are affected in Δswa2 cells, yet without compromising invasive growth. Both the TPR and J-domains of Swa2p, but not its clathrin-binding and ubiquitin-association motifs, are required for its function in Flo11p processing.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2010.02.008</identifier><identifier>PMID: 20138883</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Adaptor Proteins, Vesicular Transport ; Adaptor Proteins, Vesicular Transport - metabolism ; Auxilin ; Binding Sites ; Binding Sites - genetics ; Cell Adhesion ; Cell Adhesion - genetics ; Cell Adhesion - physiology ; Cell Communication ; Cell Communication - genetics ; Cell Communication - physiology ; Clathrin ; Clathrin - metabolism ; Cognitive Sciences ; Flo11 ; Fungal biofilm ; Gene Deletion ; Life Sciences ; Membrane Glycoproteins ; Membrane Glycoproteins - metabolism ; Neurobiology ; Neurons and Cognition ; Organisms, Genetically Modified ; Phosphoproteins ; Phosphoproteins - chemistry ; Phosphoproteins - genetics ; Phosphoproteins - metabolism ; Phosphoproteins - physiology ; Protein Binding ; Protein Binding - genetics ; Protein Processing, Post-Translational ; Protein Processing, Post-Translational - genetics ; Protein Structure, Tertiary ; Protein Structure, Tertiary - genetics ; Protein Structure, Tertiary - physiology ; Psychology and behavior ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - growth & development ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae - physiology ; Saccharomyces cerevisiae Proteins ; Saccharomyces cerevisiae Proteins - chemistry ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Saccharomyces cerevisiae Proteins - physiology ; Swa2 ; Ubiquitin ; Ubiquitin - metabolism ; Vesicular Transport Proteins ; Vesicular Transport Proteins - chemistry ; Vesicular Transport Proteins - genetics ; Vesicular Transport Proteins - metabolism ; Vesicular Transport Proteins - physiology ; Ydj1</subject><ispartof>FEBS letters, 2010-03, Vol.584 (6), p.1149-1155</ispartof><rights>2010 Federation of European Biochemical Societies</rights><rights>FEBS Letters 584 (2010) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>Copyright 2010 Federation of European Biochemical Societies. 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Mat formation strictly depends on Flo11p, a cell surface mannoprotein that mediates the adhesion of yeast cells to the agar surface. Here, we show that Swa2p, an auxilin ortholog required for clathrin-coated vesicle uncoating, is strictly required for biofilm formation. We show that the maturation and cellular levels of Flo11p are affected in Δswa2 cells, yet without compromising invasive growth. Both the TPR and J-domains of Swa2p, but not its clathrin-binding and ubiquitin-association motifs, are required for its function in Flo11p processing.</description><subject>Adaptor Proteins, Vesicular Transport</subject><subject>Adaptor Proteins, Vesicular Transport - metabolism</subject><subject>Auxilin</subject><subject>Binding Sites</subject><subject>Binding Sites - genetics</subject><subject>Cell Adhesion</subject><subject>Cell Adhesion - genetics</subject><subject>Cell Adhesion - physiology</subject><subject>Cell Communication</subject><subject>Cell Communication - genetics</subject><subject>Cell Communication - physiology</subject><subject>Clathrin</subject><subject>Clathrin - metabolism</subject><subject>Cognitive Sciences</subject><subject>Flo11</subject><subject>Fungal biofilm</subject><subject>Gene Deletion</subject><subject>Life Sciences</subject><subject>Membrane Glycoproteins</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Neurobiology</subject><subject>Neurons and Cognition</subject><subject>Organisms, Genetically Modified</subject><subject>Phosphoproteins</subject><subject>Phosphoproteins - chemistry</subject><subject>Phosphoproteins - genetics</subject><subject>Phosphoproteins - metabolism</subject><subject>Phosphoproteins - physiology</subject><subject>Protein Binding</subject><subject>Protein Binding - genetics</subject><subject>Protein Processing, Post-Translational</subject><subject>Protein Processing, Post-Translational - genetics</subject><subject>Protein Structure, Tertiary</subject><subject>Protein Structure, Tertiary - genetics</subject><subject>Protein Structure, Tertiary - physiology</subject><subject>Psychology and behavior</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - growth & development</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae - physiology</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Saccharomyces cerevisiae Proteins - chemistry</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Saccharomyces cerevisiae Proteins - physiology</subject><subject>Swa2</subject><subject>Ubiquitin</subject><subject>Ubiquitin - metabolism</subject><subject>Vesicular Transport Proteins</subject><subject>Vesicular Transport Proteins - chemistry</subject><subject>Vesicular Transport Proteins - genetics</subject><subject>Vesicular Transport Proteins - metabolism</subject><subject>Vesicular Transport Proteins - physiology</subject><subject>Ydj1</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAURi0EokPhEUDeIRYZ7Dg_zgq1VaeDNIjFdNGd5dg3jEdJHGzPVNmVt6Cv1yfBUYZuYWXZOt9nXx-E3lOypIQWn_fLBmrfQlimJJ6RdEkIf4EWlJcsYVnBX6IFITRL8rJiZ-iN93sS95xWr9FZjDDOOVugX9t7mQ6JhgF6DX3AqpVh50yP9djLziiPjcfKmWCUbHFjHV61ltIBD84q8N70P7DsNX56-P1NhqeHx4npZDC2x7El7ACPIH3AW6nUTjrbjTGGFTg4Gm8kvEWvGtl6eHdaz9Ht6vr2ap1svt98vbrYJCor2F1SQU2AMVbzqkpJWkpV15oBo1nGiqLWdRXnyTgnpCxzXXEoWNlkQLTmDPKCnaNPc-1OtmJwppNuFFYasb7YiOmMUMpZSvmRRvbjzMYZfx7AB9EZr6BtZQ_24EXJWMnLosgjmc-kctZ7B81zNSVi8iT24uRJTJ4ESUX0FHMfTjcc6g70c-qvmAisZ-DetDD-X6tYXV-m20n65JxOukl6F6u-zFUQf_dowAmvDPQKtHGggtDW_OO1fwAdFb4Q</recordid><startdate>20100319</startdate><enddate>20100319</enddate><creator>Martineau, Céline N.</creator><creator>Melki, Ronald</creator><creator>Kabani, Mehdi</creator><general>Elsevier B.V</general><general>Wiley</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-7440-6394</orcidid></search><sort><creationdate>20100319</creationdate><title>Swa2p-dependent clathrin dynamics is critical for Flo11p processing and ‘Mat’ formation in the yeast Saccharomyces cerevisiae</title><author>Martineau, Céline N. ; Melki, Ronald ; Kabani, Mehdi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463X-9eb0e333b8992027acbbd3e3144366bdb988348800775d98e637f4e0dd83e563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adaptor Proteins, Vesicular Transport</topic><topic>Adaptor Proteins, Vesicular Transport - metabolism</topic><topic>Auxilin</topic><topic>Binding Sites</topic><topic>Binding Sites - genetics</topic><topic>Cell Adhesion</topic><topic>Cell Adhesion - genetics</topic><topic>Cell Adhesion - physiology</topic><topic>Cell Communication</topic><topic>Cell Communication - genetics</topic><topic>Cell Communication - physiology</topic><topic>Clathrin</topic><topic>Clathrin - metabolism</topic><topic>Cognitive Sciences</topic><topic>Flo11</topic><topic>Fungal biofilm</topic><topic>Gene Deletion</topic><topic>Life Sciences</topic><topic>Membrane Glycoproteins</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Neurobiology</topic><topic>Neurons and Cognition</topic><topic>Organisms, Genetically Modified</topic><topic>Phosphoproteins</topic><topic>Phosphoproteins - chemistry</topic><topic>Phosphoproteins - genetics</topic><topic>Phosphoproteins - metabolism</topic><topic>Phosphoproteins - physiology</topic><topic>Protein Binding</topic><topic>Protein Binding - genetics</topic><topic>Protein Processing, Post-Translational</topic><topic>Protein Processing, Post-Translational - genetics</topic><topic>Protein Structure, Tertiary</topic><topic>Protein Structure, Tertiary - genetics</topic><topic>Protein Structure, Tertiary - physiology</topic><topic>Psychology and behavior</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - growth & development</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae - physiology</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Saccharomyces cerevisiae Proteins - chemistry</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Saccharomyces cerevisiae Proteins - physiology</topic><topic>Swa2</topic><topic>Ubiquitin</topic><topic>Ubiquitin - metabolism</topic><topic>Vesicular Transport Proteins</topic><topic>Vesicular Transport Proteins - chemistry</topic><topic>Vesicular Transport Proteins - genetics</topic><topic>Vesicular Transport Proteins - metabolism</topic><topic>Vesicular Transport Proteins - physiology</topic><topic>Ydj1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martineau, Céline N.</creatorcontrib><creatorcontrib>Melki, Ronald</creatorcontrib><creatorcontrib>Kabani, Mehdi</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martineau, Céline N.</au><au>Melki, Ronald</au><au>Kabani, Mehdi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Swa2p-dependent clathrin dynamics is critical for Flo11p processing and ‘Mat’ formation in the yeast Saccharomyces cerevisiae</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2010-03-19</date><risdate>2010</risdate><volume>584</volume><issue>6</issue><spage>1149</spage><epage>1155</epage><pages>1149-1155</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The yeast Saccharomyces cerevisiae is able to form complex multicellular structures called mats on low-density agar Petri plates. Mat formation strictly depends on Flo11p, a cell surface mannoprotein that mediates the adhesion of yeast cells to the agar surface. Here, we show that Swa2p, an auxilin ortholog required for clathrin-coated vesicle uncoating, is strictly required for biofilm formation. We show that the maturation and cellular levels of Flo11p are affected in Δswa2 cells, yet without compromising invasive growth. Both the TPR and J-domains of Swa2p, but not its clathrin-binding and ubiquitin-association motifs, are required for its function in Flo11p processing.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>20138883</pmid><doi>10.1016/j.febslet.2010.02.008</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-7440-6394</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adaptor Proteins, Vesicular Transport Adaptor Proteins, Vesicular Transport - metabolism Auxilin Binding Sites Binding Sites - genetics Cell Adhesion Cell Adhesion - genetics Cell Adhesion - physiology Cell Communication Cell Communication - genetics Cell Communication - physiology Clathrin Clathrin - metabolism Cognitive Sciences Flo11 Fungal biofilm Gene Deletion Life Sciences Membrane Glycoproteins Membrane Glycoproteins - metabolism Neurobiology Neurons and Cognition Organisms, Genetically Modified Phosphoproteins Phosphoproteins - chemistry Phosphoproteins - genetics Phosphoproteins - metabolism Phosphoproteins - physiology Protein Binding Protein Binding - genetics Protein Processing, Post-Translational Protein Processing, Post-Translational - genetics Protein Structure, Tertiary Protein Structure, Tertiary - genetics Protein Structure, Tertiary - physiology Psychology and behavior Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae - physiology Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae Proteins - chemistry Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Saccharomyces cerevisiae Proteins - physiology Swa2 Ubiquitin Ubiquitin - metabolism Vesicular Transport Proteins Vesicular Transport Proteins - chemistry Vesicular Transport Proteins - genetics Vesicular Transport Proteins - metabolism Vesicular Transport Proteins - physiology Ydj1 |
| title | Swa2p-dependent clathrin dynamics is critical for Flo11p processing and ‘Mat’ formation in the yeast Saccharomyces cerevisiae |
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