Differences in the metabolomic signatures of porcine follicular fluid collected from environments associated with good and poor oocyte quality
The microenvironment of the developing follicle is critical to the acquisition of oocyte developmental competence, which is influenced by several factors including follicle size and season. The aim of this study was to characterise the metabolomic signatures of porcine follicular fluid (FF) collecte...
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| Veröffentlicht in: | Reproduction (Cambridge, England) England), 2013-09, Vol.146 (3), p.221-231 |
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| creator | Bertoldo, Michael J Nadal-Desbarats, Lydie Gérard, Nadine Dubois, Alexis Holyoake, Patricia K Grupen, Christopher G |
| description | The microenvironment of the developing follicle is critical to the acquisition of oocyte developmental competence, which is influenced by several factors including follicle size and season. The aim of this study was to characterise the metabolomic signatures of porcine follicular fluid (FF) collected from good and poor follicular environments, using high-resolution proton nuclear magnetic resonance (1H-NMR) spectroscopy. Sow ovaries were collected at slaughter, 4 days after weaning, in summer and winter. The contents of small (3–4 mm) and large (5–8 mm) diameter follicles were aspirated and pooled separately for each ovary pair. Groups classified as summer-small (n=8), summer-large (n=15), winter-small (n=9) and winter-large (n=15) were analysed by 1H-NMR spectroscopy. The concentrations of 11 metabolites differed due to follicle size alone (P |
| doi_str_mv | 10.1530/REP-13-0142 |
| format | Article |
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The aim of this study was to characterise the metabolomic signatures of porcine follicular fluid (FF) collected from good and poor follicular environments, using high-resolution proton nuclear magnetic resonance (1H-NMR) spectroscopy. Sow ovaries were collected at slaughter, 4 days after weaning, in summer and winter. The contents of small (3–4 mm) and large (5–8 mm) diameter follicles were aspirated and pooled separately for each ovary pair. Groups classified as summer-small (n=8), summer-large (n=15), winter-small (n=9) and winter-large (n=15) were analysed by 1H-NMR spectroscopy. The concentrations of 11 metabolites differed due to follicle size alone (P<0.05), including glucose, lactate, hypoxanthine and five amino acids. The concentrations of all these metabolites, except for glucose, were lower in large FF compared with small FF. Significant interaction effects of follicle size and season were found for the concentrations of glutamate, glycine, N-acetyl groups and uridine. Succinate was the only metabolite that differed in concentration due to season alone (P<0.05). The FF levels of progesterone, androstenedione and oestradiol were correlated with the concentrations of most of the metabolites examined. The results indicate that there is a distinct shift in follicular glucose metabolism as follicles increase in diameter and suggest that follicular cells may be more vulnerable to oxidative stress during the summer months. Our findings demonstrate the power of 1H-NMR spectroscopy to expand our understanding of the dynamic and complex microenvironment of the developing follicle.</description><identifier>ISSN: 1470-1626</identifier><identifier>EISSN: 1741-7899</identifier><identifier>DOI: 10.1530/REP-13-0142</identifier><identifier>PMID: 23801780</identifier><language>eng</language><publisher>England: BioScientifica</publisher><subject>Animals ; Female ; Follicular Fluid - metabolism ; Life Sciences ; Magnetic Resonance Spectroscopy ; Metabolome ; Multivariate Analysis ; Oocytes - physiology ; Other ; Seasons ; Steroids - metabolism ; Swine</subject><ispartof>Reproduction (Cambridge, England), 2013-09, Vol.146 (3), p.221-231</ispartof><rights>2013 Society for Reproduction and Fertility</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b402t-f084e6ddaa5142367e3c27f490c2d06aff349c90d4e07b120a45e43f13aa5bd43</citedby><cites>FETCH-LOGICAL-b402t-f084e6ddaa5142367e3c27f490c2d06aff349c90d4e07b120a45e43f13aa5bd43</cites><orcidid>0000-0003-0541-7422 ; 0000-0001-8770-6951</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23801780$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01129742$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Bertoldo, Michael J</creatorcontrib><creatorcontrib>Nadal-Desbarats, Lydie</creatorcontrib><creatorcontrib>Gérard, Nadine</creatorcontrib><creatorcontrib>Dubois, Alexis</creatorcontrib><creatorcontrib>Holyoake, Patricia K</creatorcontrib><creatorcontrib>Grupen, Christopher G</creatorcontrib><title>Differences in the metabolomic signatures of porcine follicular fluid collected from environments associated with good and poor oocyte quality</title><title>Reproduction (Cambridge, England)</title><addtitle>Reproduction</addtitle><description>The microenvironment of the developing follicle is critical to the acquisition of oocyte developmental competence, which is influenced by several factors including follicle size and season. The aim of this study was to characterise the metabolomic signatures of porcine follicular fluid (FF) collected from good and poor follicular environments, using high-resolution proton nuclear magnetic resonance (1H-NMR) spectroscopy. Sow ovaries were collected at slaughter, 4 days after weaning, in summer and winter. The contents of small (3–4 mm) and large (5–8 mm) diameter follicles were aspirated and pooled separately for each ovary pair. Groups classified as summer-small (n=8), summer-large (n=15), winter-small (n=9) and winter-large (n=15) were analysed by 1H-NMR spectroscopy. The concentrations of 11 metabolites differed due to follicle size alone (P<0.05), including glucose, lactate, hypoxanthine and five amino acids. The concentrations of all these metabolites, except for glucose, were lower in large FF compared with small FF. Significant interaction effects of follicle size and season were found for the concentrations of glutamate, glycine, N-acetyl groups and uridine. Succinate was the only metabolite that differed in concentration due to season alone (P<0.05). The FF levels of progesterone, androstenedione and oestradiol were correlated with the concentrations of most of the metabolites examined. The results indicate that there is a distinct shift in follicular glucose metabolism as follicles increase in diameter and suggest that follicular cells may be more vulnerable to oxidative stress during the summer months. Our findings demonstrate the power of 1H-NMR spectroscopy to expand our understanding of the dynamic and complex microenvironment of the developing follicle.</description><subject>Animals</subject><subject>Female</subject><subject>Follicular Fluid - metabolism</subject><subject>Life Sciences</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Metabolome</subject><subject>Multivariate Analysis</subject><subject>Oocytes - physiology</subject><subject>Other</subject><subject>Seasons</subject><subject>Steroids - metabolism</subject><subject>Swine</subject><issn>1470-1626</issn><issn>1741-7899</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhiMEoh9w4o58BKGAx3bi5Fi1pUVaCYTgbDn2uGvkxFvbKdo_wW_Gqy09cvJo5vEjzbxN8wboR-g4_fT9-lsLvKUg2LPmFKSAVg7j-LzWQtIWetafNGc5_6IUukH2L5sTxgcKcqCnzZ8r7xwmXAxm4hdStkhmLHqKIc7ekOzvFl3WVKfRkV1Mxi9IXAzBmzXoRFxYvSWmNtAUtMSlOBNcHnyKy4xLyUTnHI3Xh-FvX7bkLkZL9GKrLSYSo9kXJPerDr7sXzUvnA4ZXz--583Pz9c_Lm_bzdebL5cXm3YSlJXW0UFgb63WXd2a9xK5YdKJkRpmaa-d42I0I7UCqZyAUS06FNwBrz8mK_h58_7o3eqgdsnPOu1V1F7dXmzUoUcB2CgFe4DKvjuyuxTvV8xFzT4bDEEvGNesQIDs2EgZreiHI2pSzDmhe3IDVYewVA1LAVeHsCr99lG8TjPaJ_ZfOhWAIzD5mI2v1_TOG_1f6V9lHaHx</recordid><startdate>20130901</startdate><enddate>20130901</enddate><creator>Bertoldo, Michael J</creator><creator>Nadal-Desbarats, Lydie</creator><creator>Gérard, Nadine</creator><creator>Dubois, Alexis</creator><creator>Holyoake, Patricia K</creator><creator>Grupen, Christopher G</creator><general>BioScientifica</general><general>BioScientifica [2001-....]</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0003-0541-7422</orcidid><orcidid>https://orcid.org/0000-0001-8770-6951</orcidid></search><sort><creationdate>20130901</creationdate><title>Differences in the metabolomic signatures of porcine follicular fluid collected from environments associated with good and poor oocyte quality</title><author>Bertoldo, Michael J ; Nadal-Desbarats, Lydie ; Gérard, Nadine ; Dubois, Alexis ; Holyoake, Patricia K ; Grupen, Christopher G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b402t-f084e6ddaa5142367e3c27f490c2d06aff349c90d4e07b120a45e43f13aa5bd43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Female</topic><topic>Follicular Fluid - metabolism</topic><topic>Life Sciences</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Metabolome</topic><topic>Multivariate Analysis</topic><topic>Oocytes - physiology</topic><topic>Other</topic><topic>Seasons</topic><topic>Steroids - metabolism</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bertoldo, Michael J</creatorcontrib><creatorcontrib>Nadal-Desbarats, Lydie</creatorcontrib><creatorcontrib>Gérard, Nadine</creatorcontrib><creatorcontrib>Dubois, Alexis</creatorcontrib><creatorcontrib>Holyoake, Patricia K</creatorcontrib><creatorcontrib>Grupen, Christopher G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Reproduction (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bertoldo, Michael J</au><au>Nadal-Desbarats, Lydie</au><au>Gérard, Nadine</au><au>Dubois, Alexis</au><au>Holyoake, Patricia K</au><au>Grupen, Christopher G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differences in the metabolomic signatures of porcine follicular fluid collected from environments associated with good and poor oocyte quality</atitle><jtitle>Reproduction (Cambridge, England)</jtitle><addtitle>Reproduction</addtitle><date>2013-09-01</date><risdate>2013</risdate><volume>146</volume><issue>3</issue><spage>221</spage><epage>231</epage><pages>221-231</pages><issn>1470-1626</issn><eissn>1741-7899</eissn><abstract>The microenvironment of the developing follicle is critical to the acquisition of oocyte developmental competence, which is influenced by several factors including follicle size and season. 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Succinate was the only metabolite that differed in concentration due to season alone (P<0.05). The FF levels of progesterone, androstenedione and oestradiol were correlated with the concentrations of most of the metabolites examined. The results indicate that there is a distinct shift in follicular glucose metabolism as follicles increase in diameter and suggest that follicular cells may be more vulnerable to oxidative stress during the summer months. Our findings demonstrate the power of 1H-NMR spectroscopy to expand our understanding of the dynamic and complex microenvironment of the developing follicle.</abstract><cop>England</cop><pub>BioScientifica</pub><pmid>23801780</pmid><doi>10.1530/REP-13-0142</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-0541-7422</orcidid><orcidid>https://orcid.org/0000-0001-8770-6951</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Female Follicular Fluid - metabolism Life Sciences Magnetic Resonance Spectroscopy Metabolome Multivariate Analysis Oocytes - physiology Other Seasons Steroids - metabolism Swine |
| title | Differences in the metabolomic signatures of porcine follicular fluid collected from environments associated with good and poor oocyte quality |
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