Nation-wide study of the occurrence of Listeria monocytogenes in French soils using culture-based and molecular detection methods

Soil is a potential reservoir of human pathogens and a possible source of contamination of animals, crops and water. In order to study the distribution of Listeria monocytogenes in French soils, a real-time PCR TaqMan assay targeting the phosphoribosylpyrophosphate synthetase (prs) gene of L. monocy...

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Veröffentlicht in:	Journal of microbiological methods 2013-06, Vol.93 (3), p.242-250
Hauptverfasser:	Locatelli, Aude, Depret, Géraldine, Jolivet, Claudy, Henry, Sonia, Dequiedt, Samuel, Piveteau, Pascal, Hartmann, Alain
Format:	Artikel
Sprache:	eng
Schlagworte:	bacteria Bacterial Typing Techniques Bacteriological Techniques - methods Biodiversity and Ecology Colony Count, Microbial Continental interfaces, environment cows crops Culture-based detection DNA Primers - genetics DNA, Bacterial - genetics Environmental Sciences forest soils France French soil monitoring network genes grassland soils humans Listeria monocytogenes Listeria monocytogenes - classification Listeria monocytogenes - genetics Listeria monocytogenes - isolation & purification meadows Molecular detection monitoring pastures pathogens quantitative polymerase chain reaction Real-time PCR Real-Time Polymerase Chain Reaction - methods Ribose-Phosphate Pyrophosphokinase - genetics Sciences of the Universe serotypes Serotyping Soil Microbiology soil quality soil sampling TaqMan type probe
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