Optimization and validation of a label-free MRM method for the quantification of cytochrome P450 isoforms in biological samples

Cytochromes P450 (CYPs) play critical roles in oxidative metabolism of many endogenous and exogenous compounds. Protein expression levels of CYPs in liver provide relevant information for a better understanding of the importance of CYPs in pharmacology and toxicology. This work aimed at establishing...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2014-08, Vol.406 (20), p.4861-4874
Hauptverfasser:	Al Ali, Ahmad, Touboul, David, Le Caër, Jean-Pierre, Schmitz-Afonso, Isabelle, Flinois, Jean-Pierre, Marchetti, Catherine, De Waziers, Isabelle, Brunelle, Alain, Laprévote, Olivier, Beaune, Philippe
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Analytical Chemistry Biochemistry Biological Biological samples Brain Characterization and Evaluation of Materials Chemical Sciences Chemistry Chemistry and Materials Science Cysteine - chemistry Cytochrome Cytochrome P-450 Cytochrome P-450 Enzyme System - metabolism Cytochromes P450 Digestion Enzymes Fluorescence Fluorescence microscopy Food Science Hep G2 Cells Humans Immunoassays Isoenzymes Labeling Laboratory Medicine Liver Mass spectrometry Measurement Metabolism Methods Microsomes, Liver - enzymology Mitochondria Mitochondria, Liver - enzymology Monitoring/Environmental Analysis Optimization Organic chemistry Peptide Fragments - analysis Peptides Pharmacology Physiological aspects Protein expression Proteins Quality control Recombinant Recombinant Proteins - metabolism Reproducibility Research Paper Scientific imaging Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Toxicology
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