A more consistent method for extracting and amplifying DNA from bee wings
Non-lethal sampling of DNA from honeybees is commonly required for genotyping certain behavioural traits required for breeding. One method is to use wing clippings. However, the sample is very small, and the extraction process can be difficult, resulting in low polymerase chain reaction (PCR) amplif...
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| Veröffentlicht in: | Apidologie 2011-11, Vol.42 (6), p.721-727 |
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| container_title | Apidologie |
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| creator | Gould, Elaine M. Taylor, Michelle A. Holmes, Selena J. |
| description | Non-lethal sampling of DNA from honeybees is commonly required for genotyping certain behavioural traits required for breeding. One method is to use wing clippings. However, the sample is very small, and the extraction process can be difficult, resulting in low polymerase chain reaction (PCR) amplification. Here, we describe an improved method for extracting DNA from bee wings using a commercially available kit that uses bashing beads to physically break up the wings. It enabled 93% to 100% PCR amplification when amplified using microsatellite or mitochondrial primers. The results were compared with the Chelex® 100 extraction method, which, in this laboratory, resulted in only between 56% and 72% amplification in subsequent PCR. |
| doi_str_mv | 10.1007/s13592-011-0077-x |
| format | Article |
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One method is to use wing clippings. However, the sample is very small, and the extraction process can be difficult, resulting in low polymerase chain reaction (PCR) amplification. Here, we describe an improved method for extracting DNA from bee wings using a commercially available kit that uses bashing beads to physically break up the wings. It enabled 93% to 100% PCR amplification when amplified using microsatellite or mitochondrial primers. The results were compared with the Chelex® 100 extraction method, which, in this laboratory, resulted in only between 56% and 72% amplification in subsequent PCR.</description><identifier>ISSN: 0044-8435</identifier><identifier>EISSN: 1297-9678</identifier><identifier>DOI: 10.1007/s13592-011-0077-x</identifier><identifier>CODEN: APDGB5</identifier><language>eng</language><publisher>Paris: Springer-Verlag</publisher><subject>Agricultural sciences ; Animal biology ; Animal production studies ; Animal productions ; Apiculture ; Apis mellifera ; Biodiversity ; Biological and medical sciences ; Biomedical and Life Sciences ; Breeding ; Ecology, environment ; Entomology ; Fundamental and applied biological sciences. Psychology ; General aspects and techniques. Study of several systematic groups. 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One method is to use wing clippings. However, the sample is very small, and the extraction process can be difficult, resulting in low polymerase chain reaction (PCR) amplification. Here, we describe an improved method for extracting DNA from bee wings using a commercially available kit that uses bashing beads to physically break up the wings. It enabled 93% to 100% PCR amplification when amplified using microsatellite or mitochondrial primers. The results were compared with the Chelex® 100 extraction method, which, in this laboratory, resulted in only between 56% and 72% amplification in subsequent PCR.</description><subject>Agricultural sciences</subject><subject>Animal biology</subject><subject>Animal production studies</subject><subject>Animal productions</subject><subject>Apiculture</subject><subject>Apis mellifera</subject><subject>Biodiversity</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Breeding</subject><subject>Ecology, environment</subject><subject>Entomology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Genotyping</subject><subject>Insecta</subject><subject>Invertebrate Zoology</subject><subject>Invertebrates</subject><subject>Life Sciences</subject><subject>Microsatellites</subject><subject>Miscellaneous techniques</subject><subject>Mitochondria</subject><subject>Original Article</subject><subject>Polymerase chain reaction</subject><subject>Primers</subject><subject>Sampling</subject><subject>Techniques</subject><subject>Terrestrial animal productions</subject><subject>Wings</subject><issn>0044-8435</issn><issn>1297-9678</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp9kD1PwzAURS0EEqXwA9i8IMEQ8Fcce4zKRytVsMBsOY7TpkriYqfQ_nscperI9HTfO-8MF4BbjB4xQtlTwDSVJEEYJzFmyf4MTDCRWSJ5Js7BBCHGEsFoegmuQtgghIlI2QQsctg6b6FxXahDb7setrZfuxJWzkO77702fd2toO5KqNttU1eHIT6_57DyroWFtfA3bsI1uKh0E-zNcU7B1-vL52yeLD_eFrN8mRhGRJ_oVIiCM0qkzpgkWSWwoBYxKjnJmNCYlyXNqOaFLNJKpkhwXcTJiTTWoIJOwcPoXetGbX3dan9QTtdqni_VsEOxEMox-8GRvR_ZrXffOxt61dbB2KbRnXW7oDCiTBDOKYkoHlHjXQjeVic3RmqoWI0VRz1WQ8VqH3_ujnodjG4qrztTh9MjSYkUlPDIkZEL8dStrFcbt_NdbOkf-R_8KYl5</recordid><startdate>20111101</startdate><enddate>20111101</enddate><creator>Gould, Elaine M.</creator><creator>Taylor, Michelle A.</creator><creator>Holmes, Selena J.</creator><general>Springer-Verlag</general><general>EDP Sciences</general><general>Springer Verlag</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TM</scope><scope>1XC</scope><scope>VOOES</scope></search><sort><creationdate>20111101</creationdate><title>A more consistent method for extracting and amplifying DNA from bee wings</title><author>Gould, Elaine M. ; Taylor, Michelle A. ; Holmes, Selena J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-a588b64329a74927f8183e043962748a16dd373a6b9b5f95086abf95629cec0b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Agricultural sciences</topic><topic>Animal biology</topic><topic>Animal production studies</topic><topic>Animal productions</topic><topic>Apiculture</topic><topic>Apis mellifera</topic><topic>Biodiversity</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Breeding</topic><topic>Ecology, environment</topic><topic>Entomology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>Genotyping</topic><topic>Insecta</topic><topic>Invertebrate Zoology</topic><topic>Invertebrates</topic><topic>Life Sciences</topic><topic>Microsatellites</topic><topic>Miscellaneous techniques</topic><topic>Mitochondria</topic><topic>Original Article</topic><topic>Polymerase chain reaction</topic><topic>Primers</topic><topic>Sampling</topic><topic>Techniques</topic><topic>Terrestrial animal productions</topic><topic>Wings</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gould, Elaine M.</creatorcontrib><creatorcontrib>Taylor, Michelle A.</creatorcontrib><creatorcontrib>Holmes, Selena J.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Apidologie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gould, Elaine M.</au><au>Taylor, Michelle A.</au><au>Holmes, Selena J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A more consistent method for extracting and amplifying DNA from bee wings</atitle><jtitle>Apidologie</jtitle><stitle>Apidologie</stitle><date>2011-11-01</date><risdate>2011</risdate><volume>42</volume><issue>6</issue><spage>721</spage><epage>727</epage><pages>721-727</pages><issn>0044-8435</issn><eissn>1297-9678</eissn><coden>APDGB5</coden><abstract>Non-lethal sampling of DNA from honeybees is commonly required for genotyping certain behavioural traits required for breeding. One method is to use wing clippings. However, the sample is very small, and the extraction process can be difficult, resulting in low polymerase chain reaction (PCR) amplification. Here, we describe an improved method for extracting DNA from bee wings using a commercially available kit that uses bashing beads to physically break up the wings. It enabled 93% to 100% PCR amplification when amplified using microsatellite or mitochondrial primers. The results were compared with the Chelex® 100 extraction method, which, in this laboratory, resulted in only between 56% and 72% amplification in subsequent PCR.</abstract><cop>Paris</cop><pub>Springer-Verlag</pub><doi>10.1007/s13592-011-0077-x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | SpringerNature Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Agricultural sciences Animal biology Animal production studies Animal productions Apiculture Apis mellifera Biodiversity Biological and medical sciences Biomedical and Life Sciences Breeding Ecology, environment Entomology Fundamental and applied biological sciences. Psychology General aspects and techniques. Study of several systematic groups. Models Genotyping Insecta Invertebrate Zoology Invertebrates Life Sciences Microsatellites Miscellaneous techniques Mitochondria Original Article Polymerase chain reaction Primers Sampling Techniques Terrestrial animal productions Wings |
| title | A more consistent method for extracting and amplifying DNA from bee wings |
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