Nonstructural protein NS1 immunodominant epitope detected specifically in dengue virus infected material by a SELDI-TOF/MS based assay
Dengue virus (DV) infection is the most common mosquito‐born viral disease of public health significance. Though most patients only suffer from flu‐like symptoms, a small group of patients experiences more severe forms of the disease. The viral nonstructural protein 1 (NS1), a secreted protein corre...
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| Veröffentlicht in: | Journal of medical virology 2012-03, Vol.84 (3), p.490-499 |
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| creator | Steidel, Marine Fragnoud, Romain Guillotte, Michelle Roesch, Céline Michel, Sandrine Meunier, Thomas Paranhos-Baccalà, Glàucia Gervasi, Gaspard Bedin, Frederic |
| description | Dengue virus (DV) infection is the most common mosquito‐born viral disease of public health significance. Though most patients only suffer from flu‐like symptoms, a small group of patients experiences more severe forms of the disease. The viral nonstructural protein 1 (NS1), a secreted protein correlating with viremia, is a key element used for dengue diagnosis with potential implications in severe dengue prognosis. Capture‐ELISAs for the early detection of the NS1 protein in the sera during the acute febrile stage are commonly used in routine by diagnostic laboratories. In this study, the detection of NS1 protein in DV‐infected material was assessed by an alternative method combining a single NS1‐directed monoclonal antibody and the SELDI‐TOF/MS technology. According to the epitope mapping, the antibodies used are mainly directed against an immuno‐dominant peptide located on the C‐terminal part of the protein. The NS1 SELDI‐TOF assay is specific, has a sensitivity level close to capture‐ELISAs and is potentially useful for a coupled serotyping/detection assay or for the detection of subtle post‐translational modifications on the protein. J. Med. Virol. 84:490–499, 2012. © 2011 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/jmv.23204 |
| format | Article |
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Though most patients only suffer from flu‐like symptoms, a small group of patients experiences more severe forms of the disease. The viral nonstructural protein 1 (NS1), a secreted protein correlating with viremia, is a key element used for dengue diagnosis with potential implications in severe dengue prognosis. Capture‐ELISAs for the early detection of the NS1 protein in the sera during the acute febrile stage are commonly used in routine by diagnostic laboratories. In this study, the detection of NS1 protein in DV‐infected material was assessed by an alternative method combining a single NS1‐directed monoclonal antibody and the SELDI‐TOF/MS technology. According to the epitope mapping, the antibodies used are mainly directed against an immuno‐dominant peptide located on the C‐terminal part of the protein. The NS1 SELDI‐TOF assay is specific, has a sensitivity level close to capture‐ELISAs and is potentially useful for a coupled serotyping/detection assay or for the detection of subtle post‐translational modifications on the protein. J. Med. Virol. 84:490–499, 2012. © 2011 Wiley Periodicals, Inc.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.23204</identifier><identifier>PMID: 22246837</identifier><identifier>CODEN: JMVIDB</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adolescent ; Amino Acid Sequence ; Animals ; Antibodies, Monoclonal ; Antibodies, Monoclonal - immunology ; Antibodies, Viral ; Antibodies, Viral - immunology ; Biological and medical sciences ; Cercopithecus aethiops ; Child ; Child, Preschool ; Dengue ; Dengue - diagnosis ; Dengue - immunology ; Dengue Virus ; Dengue Virus - immunology ; Epitope Mapping ; Female ; Fundamental and applied biological sciences. Psychology ; HEK293 Cells ; Human viral diseases ; Humans ; Immunodominant Epitopes ; Immunodominant Epitopes - chemistry ; Immunodominant Epitopes - immunology ; Infant ; Infectious diseases ; Life Sciences ; Male ; Medical sciences ; Microbiology ; Miscellaneous ; Molecular Sequence Data ; NS1 ; Reproducibility of Results ; SELDI-TOF ; Sensitivity and Specificity ; severe dengue ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Vero Cells ; Viral diseases ; Viral Nonstructural Proteins ; Viral Nonstructural Proteins - chemistry ; Viral Nonstructural Proteins - immunology ; Virology</subject><ispartof>Journal of medical virology, 2012-03, Vol.84 (3), p.490-499</ispartof><rights>Copyright © 2012 Wiley Periodicals, Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5534-4c3040c9d542a76c55502f92f220f5d10b48782f64f808c6d77932e4b00c94173</citedby><cites>FETCH-LOGICAL-c5534-4c3040c9d542a76c55502f92f220f5d10b48782f64f808c6d77932e4b00c94173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmv.23204$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmv.23204$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,315,781,785,886,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25820158$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22246837$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00965843$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Steidel, Marine</creatorcontrib><creatorcontrib>Fragnoud, Romain</creatorcontrib><creatorcontrib>Guillotte, Michelle</creatorcontrib><creatorcontrib>Roesch, Céline</creatorcontrib><creatorcontrib>Michel, Sandrine</creatorcontrib><creatorcontrib>Meunier, Thomas</creatorcontrib><creatorcontrib>Paranhos-Baccalà, Glàucia</creatorcontrib><creatorcontrib>Gervasi, Gaspard</creatorcontrib><creatorcontrib>Bedin, Frederic</creatorcontrib><title>Nonstructural protein NS1 immunodominant epitope detected specifically in dengue virus infected material by a SELDI-TOF/MS based assay</title><title>Journal of medical virology</title><addtitle>J. Med. Virol</addtitle><description>Dengue virus (DV) infection is the most common mosquito‐born viral disease of public health significance. Though most patients only suffer from flu‐like symptoms, a small group of patients experiences more severe forms of the disease. The viral nonstructural protein 1 (NS1), a secreted protein correlating with viremia, is a key element used for dengue diagnosis with potential implications in severe dengue prognosis. Capture‐ELISAs for the early detection of the NS1 protein in the sera during the acute febrile stage are commonly used in routine by diagnostic laboratories. In this study, the detection of NS1 protein in DV‐infected material was assessed by an alternative method combining a single NS1‐directed monoclonal antibody and the SELDI‐TOF/MS technology. According to the epitope mapping, the antibodies used are mainly directed against an immuno‐dominant peptide located on the C‐terminal part of the protein. The NS1 SELDI‐TOF assay is specific, has a sensitivity level close to capture‐ELISAs and is potentially useful for a coupled serotyping/detection assay or for the detection of subtle post‐translational modifications on the protein. J. Med. Virol. 84:490–499, 2012. © 2011 Wiley Periodicals, Inc.</description><subject>Adolescent</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Viral</subject><subject>Antibodies, Viral - immunology</subject><subject>Biological and medical sciences</subject><subject>Cercopithecus aethiops</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Dengue</subject><subject>Dengue - diagnosis</subject><subject>Dengue - immunology</subject><subject>Dengue Virus</subject><subject>Dengue Virus - immunology</subject><subject>Epitope Mapping</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HEK293 Cells</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Immunodominant Epitopes</subject><subject>Immunodominant Epitopes - chemistry</subject><subject>Immunodominant Epitopes - immunology</subject><subject>Infant</subject><subject>Infectious diseases</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>NS1</subject><subject>Reproducibility of Results</subject><subject>SELDI-TOF</subject><subject>Sensitivity and Specificity</subject><subject>severe dengue</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>Vero Cells</subject><subject>Viral diseases</subject><subject>Viral Nonstructural Proteins</subject><subject>Viral Nonstructural Proteins - chemistry</subject><subject>Viral Nonstructural Proteins - immunology</subject><subject>Virology</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctuEzEUhi0EoiGw4AWQJVQBi2mOPbbHXlahV6WpUAosLcfjAYe5BHumkBfguXFJmkpIsLJ89P3_ufwIvSRwRADoZNXcHtGcAnuERgSUyBQU5DEaAWEiE4LwA_QsxhUASEXpU3RAKWVC5sUI_Zp3bezDYPshmBqvQ9c73-L5gmDfNEPblV3jW9P22K19360dLl3vbO9KHNfO-spbU9cbnDSla78MDt_6MMT0r7ZUY3oXfLJebrDBi5PZ-4vs5vp0crXASxMTYGI0m-foSWXq6F7s3jH6eHpyMz3PZtdnF9PjWWY5z1nGbA4MrCo5o6YQqciBVopWlELFSwJLJgtJK8EqCdKKsihUTh1bQhIxUuRj9G7r-9XUeh18Y8JGd8br8-OZvqtBOh-XLL8liX2zZdNRvg8u9rrx0bq6Nq3rhqgVEZxImTqM0dv_koQLIbkiOST09V_oqhtCm3ZOFOMghVLqYUwbuhiDq_azEtB3kesUuf4TeWJf7RyHZePKPXmfcQIOd4CJKawqmNb6-MBxSYFwmbjJlvvha7f5d0d9efXpvnW2VfjYu597hQnftCjyguvP8zO94B94Elzqaf4bdQfO4w</recordid><startdate>201203</startdate><enddate>201203</enddate><creator>Steidel, Marine</creator><creator>Fragnoud, Romain</creator><creator>Guillotte, Michelle</creator><creator>Roesch, Céline</creator><creator>Michel, Sandrine</creator><creator>Meunier, Thomas</creator><creator>Paranhos-Baccalà, Glàucia</creator><creator>Gervasi, Gaspard</creator><creator>Bedin, Frederic</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7T2</scope><scope>7T5</scope><scope>7U2</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>201203</creationdate><title>Nonstructural protein NS1 immunodominant epitope detected specifically in dengue virus infected material by a SELDI-TOF/MS based assay</title><author>Steidel, Marine ; Fragnoud, Romain ; Guillotte, Michelle ; Roesch, Céline ; Michel, Sandrine ; Meunier, Thomas ; Paranhos-Baccalà, Glàucia ; Gervasi, Gaspard ; Bedin, Frederic</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5534-4c3040c9d542a76c55502f92f220f5d10b48782f64f808c6d77932e4b00c94173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adolescent</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Viral</topic><topic>Antibodies, Viral - immunology</topic><topic>Biological and medical sciences</topic><topic>Cercopithecus aethiops</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Dengue</topic><topic>Dengue - diagnosis</topic><topic>Dengue - immunology</topic><topic>Dengue Virus</topic><topic>Dengue Virus - immunology</topic><topic>Epitope Mapping</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HEK293 Cells</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Immunodominant Epitopes</topic><topic>Immunodominant Epitopes - chemistry</topic><topic>Immunodominant Epitopes - immunology</topic><topic>Infant</topic><topic>Infectious diseases</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>NS1</topic><topic>Reproducibility of Results</topic><topic>SELDI-TOF</topic><topic>Sensitivity and Specificity</topic><topic>severe dengue</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><topic>Vero Cells</topic><topic>Viral diseases</topic><topic>Viral Nonstructural Proteins</topic><topic>Viral Nonstructural Proteins - chemistry</topic><topic>Viral Nonstructural Proteins - immunology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Steidel, Marine</creatorcontrib><creatorcontrib>Fragnoud, Romain</creatorcontrib><creatorcontrib>Guillotte, Michelle</creatorcontrib><creatorcontrib>Roesch, Céline</creatorcontrib><creatorcontrib>Michel, Sandrine</creatorcontrib><creatorcontrib>Meunier, Thomas</creatorcontrib><creatorcontrib>Paranhos-Baccalà, Glàucia</creatorcontrib><creatorcontrib>Gervasi, Gaspard</creatorcontrib><creatorcontrib>Bedin, Frederic</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Safety Science and Risk</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Steidel, Marine</au><au>Fragnoud, Romain</au><au>Guillotte, Michelle</au><au>Roesch, Céline</au><au>Michel, Sandrine</au><au>Meunier, Thomas</au><au>Paranhos-Baccalà, Glàucia</au><au>Gervasi, Gaspard</au><au>Bedin, Frederic</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nonstructural protein NS1 immunodominant epitope detected specifically in dengue virus infected material by a SELDI-TOF/MS based assay</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J. Med. Virol</addtitle><date>2012-03</date><risdate>2012</risdate><volume>84</volume><issue>3</issue><spage>490</spage><epage>499</epage><pages>490-499</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><coden>JMVIDB</coden><abstract>Dengue virus (DV) infection is the most common mosquito‐born viral disease of public health significance. Though most patients only suffer from flu‐like symptoms, a small group of patients experiences more severe forms of the disease. The viral nonstructural protein 1 (NS1), a secreted protein correlating with viremia, is a key element used for dengue diagnosis with potential implications in severe dengue prognosis. Capture‐ELISAs for the early detection of the NS1 protein in the sera during the acute febrile stage are commonly used in routine by diagnostic laboratories. In this study, the detection of NS1 protein in DV‐infected material was assessed by an alternative method combining a single NS1‐directed monoclonal antibody and the SELDI‐TOF/MS technology. According to the epitope mapping, the antibodies used are mainly directed against an immuno‐dominant peptide located on the C‐terminal part of the protein. The NS1 SELDI‐TOF assay is specific, has a sensitivity level close to capture‐ELISAs and is potentially useful for a coupled serotyping/detection assay or for the detection of subtle post‐translational modifications on the protein. J. Med. Virol. 84:490–499, 2012. © 2011 Wiley Periodicals, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>22246837</pmid><doi>10.1002/jmv.23204</doi><tpages>10</tpages></addata></record> |
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| ispartof | Journal of medical virology, 2012-03, Vol.84 (3), p.490-499 |
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| subjects | Adolescent Amino Acid Sequence Animals Antibodies, Monoclonal Antibodies, Monoclonal - immunology Antibodies, Viral Antibodies, Viral - immunology Biological and medical sciences Cercopithecus aethiops Child Child, Preschool Dengue Dengue - diagnosis Dengue - immunology Dengue Virus Dengue Virus - immunology Epitope Mapping Female Fundamental and applied biological sciences. Psychology HEK293 Cells Human viral diseases Humans Immunodominant Epitopes Immunodominant Epitopes - chemistry Immunodominant Epitopes - immunology Infant Infectious diseases Life Sciences Male Medical sciences Microbiology Miscellaneous Molecular Sequence Data NS1 Reproducibility of Results SELDI-TOF Sensitivity and Specificity severe dengue Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Vero Cells Viral diseases Viral Nonstructural Proteins Viral Nonstructural Proteins - chemistry Viral Nonstructural Proteins - immunology Virology |
| title | Nonstructural protein NS1 immunodominant epitope detected specifically in dengue virus infected material by a SELDI-TOF/MS based assay |
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