Micriamoeba tesseris nov. gen. nov. sp.: A New Taxon of Free-living Small-sized Amoebae Non-permissive to Virulent Legionellae
Investigation of soil amoebae in 11 cooling towers allowed us to isolate a major unknown small-sized amoeba population (SZA). However, SZA did not appear to be specific to cooling tower ecosystems since they are also a major amoeba population found in muds isolated from different points of a water t...
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| Veröffentlicht in: | Protist 2012-11, Vol.163 (6), p.888-902 |
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| creator | Atlan, Danièle Coupat-Goutaland, Bénédicte Risler, Arnaud Reyrolle, Monique Souchon, Maud Briolay, Jérôme Jarraud, Sophie Doublet, Patricia Pélandakis, Michel |
| description | Investigation of soil amoebae in 11 cooling towers allowed us to isolate a major unknown small-sized amoeba population (SZA). However, SZA did not appear to be specific to cooling tower ecosystems since they are also a major amoeba population found in muds isolated from different points of a water treatment plant. The SSU-rDNA sequences from SZA strains did not match any known database sequences, suggesting that SZA constitutes a new amoeba taxon. We isolated and further described one of the SZA that we named Micriamoeba tesseris. The phylogenetic analyses showed that Micriamoeba tesseris belongs to the Amebozoa and branched together with genus Echinamoeba+Vermamoeba vermiformis. Phylogenetic analyses within the Micriamoeba group distinguished different subgroups of Micriamoeba strains according to their origin, i.e. cooling tower or mud. Although Micriamoeba are able to feed on viable E. coli cells, they do not uptake virulent Legionella pneumophila strains, thus enabling them to avoid infection by Legionella. Consequently, Micriamoeba is not directly involved in L. pneumophila multiplication. However, an indirect role of Micriamoeba in Legionella risk is discussed. |
| doi_str_mv | 10.1016/j.protis.2012.04.006 |
| format | Article |
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However, SZA did not appear to be specific to cooling tower ecosystems since they are also a major amoeba population found in muds isolated from different points of a water treatment plant. The SSU-rDNA sequences from SZA strains did not match any known database sequences, suggesting that SZA constitutes a new amoeba taxon. We isolated and further described one of the SZA that we named Micriamoeba tesseris. The phylogenetic analyses showed that Micriamoeba tesseris belongs to the Amebozoa and branched together with genus Echinamoeba+Vermamoeba vermiformis. Phylogenetic analyses within the Micriamoeba group distinguished different subgroups of Micriamoeba strains according to their origin, i.e. cooling tower or mud. Although Micriamoeba are able to feed on viable E. coli cells, they do not uptake virulent Legionella pneumophila strains, thus enabling them to avoid infection by Legionella. Consequently, Micriamoeba is not directly involved in L. pneumophila multiplication. However, an indirect role of Micriamoeba in Legionella risk is discussed.</description><identifier>ISSN: 1434-4610</identifier><identifier>EISSN: 1618-0941</identifier><identifier>DOI: 10.1016/j.protis.2012.04.006</identifier><identifier>PMID: 22677099</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Actin ; Amoeba ; Amoeba - classification ; Amoeba - isolation & purification ; Amoeba - microbiology ; Amoebozoa ; Cluster Analysis ; cooling ; DNA, Protozoan ; DNA, Protozoan - chemistry ; DNA, Protozoan - genetics ; DNA, Ribosomal ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; ecosystems ; Environmental Microbiology ; Escherichia coli ; Escherichia coli - isolation & purification ; Feeding Behavior ; Genes, rRNA ; Legionella ; Legionella pneumophila ; Legionella pneumophila - isolation & purification ; Life Sciences ; Micriamoeba ; Molecular Sequence Data ; Phylogeny ; risk ; RNA, Protozoan ; RNA, Protozoan - genetics ; RNA, Ribosomal, 18S ; RNA, Ribosomal, 18S - genetics ; Sequence Analysis, DNA ; soil ; SSU-rDNA ; virulence ; water treatment</subject><ispartof>Protist, 2012-11, Vol.163 (6), p.888-902</ispartof><rights>2012 Elsevier GmbH</rights><rights>Copyright © 2012 Elsevier GmbH. 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However, SZA did not appear to be specific to cooling tower ecosystems since they are also a major amoeba population found in muds isolated from different points of a water treatment plant. The SSU-rDNA sequences from SZA strains did not match any known database sequences, suggesting that SZA constitutes a new amoeba taxon. We isolated and further described one of the SZA that we named Micriamoeba tesseris. The phylogenetic analyses showed that Micriamoeba tesseris belongs to the Amebozoa and branched together with genus Echinamoeba+Vermamoeba vermiformis. Phylogenetic analyses within the Micriamoeba group distinguished different subgroups of Micriamoeba strains according to their origin, i.e. cooling tower or mud. Although Micriamoeba are able to feed on viable E. coli cells, they do not uptake virulent Legionella pneumophila strains, thus enabling them to avoid infection by Legionella. Consequently, Micriamoeba is not directly involved in L. pneumophila multiplication. However, an indirect role of Micriamoeba in Legionella risk is discussed.</description><subject>Actin</subject><subject>Amoeba</subject><subject>Amoeba - classification</subject><subject>Amoeba - isolation & purification</subject><subject>Amoeba - microbiology</subject><subject>Amoebozoa</subject><subject>Cluster Analysis</subject><subject>cooling</subject><subject>DNA, Protozoan</subject><subject>DNA, Protozoan - chemistry</subject><subject>DNA, Protozoan - genetics</subject><subject>DNA, Ribosomal</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>ecosystems</subject><subject>Environmental Microbiology</subject><subject>Escherichia coli</subject><subject>Escherichia coli - isolation & purification</subject><subject>Feeding Behavior</subject><subject>Genes, rRNA</subject><subject>Legionella</subject><subject>Legionella pneumophila</subject><subject>Legionella pneumophila - isolation & purification</subject><subject>Life Sciences</subject><subject>Micriamoeba</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>risk</subject><subject>RNA, Protozoan</subject><subject>RNA, Protozoan - genetics</subject><subject>RNA, Ribosomal, 18S</subject><subject>RNA, Ribosomal, 18S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>soil</subject><subject>SSU-rDNA</subject><subject>virulence</subject><subject>water treatment</subject><issn>1434-4610</issn><issn>1618-0941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhi0EoqXwDxD4CIeEseN1bA5Iq4pSpKUc2nK1nGS8eJXEi50NHwd-O15SesQXj0bPvPPxEvKcQcmAyTe7ch_D5FPJgfESRAkgH5BTJpkqQAv2MMeiEoWQDE7Ik5R2AExoqR6TE85lXYPWp-T3J99Gb4eAjaUTpoTRJzqGuaRbHMslSvvyLV3TK_xOb-yPMNLg6EVELHo_-3FLrwfb90Xyv7Cj679SSK_CWOwxDj4lPyOdAv3i46HHcaIb3PowYt9bfEoeOdsnfHb3n5Hbi_c355fF5vOHj-frTdEKDlPRVdi6WtXKMqe6unFa17KRSjcVsyBzplopu-ISVAOV66zIj9mOc7ROg63OyOtF96vtzT76wcafJlhvLtcbc8wBaLkSTM4ss68WNt_32wHTZPIS7XHcEcMhGQaKCxC6rjIqFrSNIaWI7l6bgTm6ZHZmcckcXTIgciOZy17cdTg0A3b3Rf9sycDLBXA2GLvNlpjb66wgAUCsRKUy8W4hMF9t9hhNaj2OLXY-YjuZLvj_z_AHz5OtzA</recordid><startdate>20121101</startdate><enddate>20121101</enddate><creator>Atlan, Danièle</creator><creator>Coupat-Goutaland, Bénédicte</creator><creator>Risler, Arnaud</creator><creator>Reyrolle, Monique</creator><creator>Souchon, Maud</creator><creator>Briolay, Jérôme</creator><creator>Jarraud, Sophie</creator><creator>Doublet, Patricia</creator><creator>Pélandakis, Michel</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-0614-9848</orcidid><orcidid>https://orcid.org/0000-0001-5848-4317</orcidid><orcidid>https://orcid.org/0000-0001-5750-0215</orcidid></search><sort><creationdate>20121101</creationdate><title>Micriamoeba tesseris nov. gen. nov. sp.: A New Taxon of Free-living Small-sized Amoebae Non-permissive to Virulent Legionellae</title><author>Atlan, Danièle ; 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However, SZA did not appear to be specific to cooling tower ecosystems since they are also a major amoeba population found in muds isolated from different points of a water treatment plant. The SSU-rDNA sequences from SZA strains did not match any known database sequences, suggesting that SZA constitutes a new amoeba taxon. We isolated and further described one of the SZA that we named Micriamoeba tesseris. The phylogenetic analyses showed that Micriamoeba tesseris belongs to the Amebozoa and branched together with genus Echinamoeba+Vermamoeba vermiformis. Phylogenetic analyses within the Micriamoeba group distinguished different subgroups of Micriamoeba strains according to their origin, i.e. cooling tower or mud. Although Micriamoeba are able to feed on viable E. coli cells, they do not uptake virulent Legionella pneumophila strains, thus enabling them to avoid infection by Legionella. Consequently, Micriamoeba is not directly involved in L. pneumophila multiplication. However, an indirect role of Micriamoeba in Legionella risk is discussed.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>22677099</pmid><doi>10.1016/j.protis.2012.04.006</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-0614-9848</orcidid><orcidid>https://orcid.org/0000-0001-5848-4317</orcidid><orcidid>https://orcid.org/0000-0001-5750-0215</orcidid></addata></record> |
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| ispartof | Protist, 2012-11, Vol.163 (6), p.888-902 |
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| subjects | Actin Amoeba Amoeba - classification Amoeba - isolation & purification Amoeba - microbiology Amoebozoa Cluster Analysis cooling DNA, Protozoan DNA, Protozoan - chemistry DNA, Protozoan - genetics DNA, Ribosomal DNA, Ribosomal - chemistry DNA, Ribosomal - genetics ecosystems Environmental Microbiology Escherichia coli Escherichia coli - isolation & purification Feeding Behavior Genes, rRNA Legionella Legionella pneumophila Legionella pneumophila - isolation & purification Life Sciences Micriamoeba Molecular Sequence Data Phylogeny risk RNA, Protozoan RNA, Protozoan - genetics RNA, Ribosomal, 18S RNA, Ribosomal, 18S - genetics Sequence Analysis, DNA soil SSU-rDNA virulence water treatment |
| title | Micriamoeba tesseris nov. gen. nov. sp.: A New Taxon of Free-living Small-sized Amoebae Non-permissive to Virulent Legionellae |
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