Profiling an electrospray plume by laser-induced fluorescence and Fraunhofer diffraction combined to mass spectrometry: influence of size and composition of droplets on charge-state distributions of electrosprayed proteins
We investigated how physico-chemical properties of charged droplets are affected by the electrospray process, using simultaneous in situ measurements by laser-induced fluorescence (LIF), Fraunhofer diffraction and mass spectrometry. For this purpose, we implemented a laser-induced-fluorescence profi...
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| Veröffentlicht in: | Physical chemistry chemical physics : PCCP 2012-07, Vol.14 (26), p.9389-9396 |
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| creator | GIROD, Marion DAGANY, Xavier STAFFORD, George BOUTOU, Véronique BROYER, Michel ANTOINE, Rodolphe DUGOURD, Philippe MORDEHAI, Alex LOVE, Craig WERLICH, Mark FJELDSTED, John |
| description | We investigated how physico-chemical properties of charged droplets are affected by the electrospray process, using simultaneous in situ measurements by laser-induced fluorescence (LIF), Fraunhofer diffraction and mass spectrometry. For this purpose, we implemented a laser-induced-fluorescence profiling setup in conjunction with a fast, high-resolution particle sizing scheme on a modified Agilent Jet Stream electrospray source coupled to a single quadrupole mass analyser. The optical setup permits us to profile the solvent fractionation and the size of the droplets as they evaporate in an electrospray plume by measuring both the angular scattering pattern and emission spectra of a solvatochromic fluorescent dye. Mass spectra are recorded simultaneously. These mass spectrometry and optical spectroscopy investigations allow us to study the relation between the observed charge-state distributions of protein anions and physico-chemical properties of evaporating droplets in the spray plume. By mixing water with methanol, a refolding of cytochrome C is observed as the water percentage increases in the plume due to the preponderant evaporation of volatile methanol. |
| doi_str_mv | 10.1039/c2cp23341j |
| format | Article |
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For this purpose, we implemented a laser-induced-fluorescence profiling setup in conjunction with a fast, high-resolution particle sizing scheme on a modified Agilent Jet Stream electrospray source coupled to a single quadrupole mass analyser. The optical setup permits us to profile the solvent fractionation and the size of the droplets as they evaporate in an electrospray plume by measuring both the angular scattering pattern and emission spectra of a solvatochromic fluorescent dye. Mass spectra are recorded simultaneously. These mass spectrometry and optical spectroscopy investigations allow us to study the relation between the observed charge-state distributions of protein anions and physico-chemical properties of evaporating droplets in the spray plume. By mixing water with methanol, a refolding of cytochrome C is observed as the water percentage increases in the plume due to the preponderant evaporation of volatile methanol.</description><identifier>ISSN: 1463-9076</identifier><identifier>EISSN: 1463-9084</identifier><identifier>DOI: 10.1039/c2cp23341j</identifier><identifier>PMID: 22246045</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Chemical Sciences ; Chemistry ; Cytochromes c - chemistry ; Diffraction ; Droplets ; Evaporation ; Exact sciences and technology ; Fluorescence ; Fluorescent Dyes - chemistry ; General and physical chemistry ; Lasers ; Mass Spectrometry ; Methanol - chemistry ; Methyl alcohol ; Particle Size ; Plumes ; Profiling ; Proteins - chemistry ; Solvents - chemistry ; Spectrometry, Mass, Electrospray Ionization ; Volatile Organic Compounds - chemistry ; Water - chemistry</subject><ispartof>Physical chemistry chemical physics : PCCP, 2012-07, Vol.14 (26), p.9389-9396</ispartof><rights>2015 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-2d550df9edbdb470103890dc83372eb2c204dccb747dbc558f0bde95811889e3</citedby><cites>FETCH-LOGICAL-c384t-2d550df9edbdb470103890dc83372eb2c204dccb747dbc558f0bde95811889e3</cites><orcidid>0000-0002-9715-7404 ; 0000-0003-4395-1140 ; 0000-0001-5682-8550</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26042773$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22246045$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00947061$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>GIROD, Marion</creatorcontrib><creatorcontrib>DAGANY, Xavier</creatorcontrib><creatorcontrib>STAFFORD, George</creatorcontrib><creatorcontrib>BOUTOU, Véronique</creatorcontrib><creatorcontrib>BROYER, Michel</creatorcontrib><creatorcontrib>ANTOINE, Rodolphe</creatorcontrib><creatorcontrib>DUGOURD, Philippe</creatorcontrib><creatorcontrib>MORDEHAI, Alex</creatorcontrib><creatorcontrib>LOVE, Craig</creatorcontrib><creatorcontrib>WERLICH, Mark</creatorcontrib><creatorcontrib>FJELDSTED, John</creatorcontrib><title>Profiling an electrospray plume by laser-induced fluorescence and Fraunhofer diffraction combined to mass spectrometry: influence of size and composition of droplets on charge-state distributions of electrosprayed proteins</title><title>Physical chemistry chemical physics : PCCP</title><addtitle>Phys Chem Chem Phys</addtitle><description>We investigated how physico-chemical properties of charged droplets are affected by the electrospray process, using simultaneous in situ measurements by laser-induced fluorescence (LIF), Fraunhofer diffraction and mass spectrometry. For this purpose, we implemented a laser-induced-fluorescence profiling setup in conjunction with a fast, high-resolution particle sizing scheme on a modified Agilent Jet Stream electrospray source coupled to a single quadrupole mass analyser. The optical setup permits us to profile the solvent fractionation and the size of the droplets as they evaporate in an electrospray plume by measuring both the angular scattering pattern and emission spectra of a solvatochromic fluorescent dye. Mass spectra are recorded simultaneously. These mass spectrometry and optical spectroscopy investigations allow us to study the relation between the observed charge-state distributions of protein anions and physico-chemical properties of evaporating droplets in the spray plume. By mixing water with methanol, a refolding of cytochrome C is observed as the water percentage increases in the plume due to the preponderant evaporation of volatile methanol.</description><subject>Chemical Sciences</subject><subject>Chemistry</subject><subject>Cytochromes c - chemistry</subject><subject>Diffraction</subject><subject>Droplets</subject><subject>Evaporation</subject><subject>Exact sciences and technology</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes - chemistry</subject><subject>General and physical chemistry</subject><subject>Lasers</subject><subject>Mass Spectrometry</subject><subject>Methanol - chemistry</subject><subject>Methyl alcohol</subject><subject>Particle Size</subject><subject>Plumes</subject><subject>Profiling</subject><subject>Proteins - chemistry</subject><subject>Solvents - chemistry</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Volatile Organic Compounds - chemistry</subject><subject>Water - chemistry</subject><issn>1463-9076</issn><issn>1463-9084</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks1u1DAQgCMEoqVw4QGQL0iAFPBf_rhVFaVIK8Gh98ixJ11Xjh08TqXlYXkWnN1ly5GTrdE334xmpiheM_qRUdF90lzPXAjJ7p8U50zWouxoK5-e_k19VrxAvKeUsoqJ58UZ51zWVFbnxe8fMYzWWX9HlCfgQKcYcI5qR2a3TECGHXEKIZbWm0WDIaNbQgTU4DXkHEOuo1r8NowQibHjGJVONniiwzRYnxNSIJNCJDjv5ROkuPtMrM-ivSOMBO2vgysnzQHtXpDjJobZQUKy-rYq3kGJSSXIhTBFOywriCv5b-e55hxDAuvxZfFsVA7h1fG9KG6vv9xe3ZSb71-_XV1uSi1amUpuqoqasQMzmEE2NI-17ajRrRANh4FrTqXRemhkYwZdVe1IBwNd1TLWth2Ii-L9QbtVrp-jnVTc9UHZ_uZy068xSrusrdkDy-y7A5t7_LkApn6yeZrOKQ9hwX6tzaWoqvo_UE5bIeuGZ_TDAdV5CBhhPLXB6Krs-scryfCbo3cZJjAn9O9ZZODtEVColcs79driI5ch3jRC_AENJMtQ</recordid><startdate>20120714</startdate><enddate>20120714</enddate><creator>GIROD, Marion</creator><creator>DAGANY, Xavier</creator><creator>STAFFORD, George</creator><creator>BOUTOU, Véronique</creator><creator>BROYER, Michel</creator><creator>ANTOINE, Rodolphe</creator><creator>DUGOURD, Philippe</creator><creator>MORDEHAI, Alex</creator><creator>LOVE, Craig</creator><creator>WERLICH, Mark</creator><creator>FJELDSTED, John</creator><general>Royal Society of Chemistry</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-9715-7404</orcidid><orcidid>https://orcid.org/0000-0003-4395-1140</orcidid><orcidid>https://orcid.org/0000-0001-5682-8550</orcidid></search><sort><creationdate>20120714</creationdate><title>Profiling an electrospray plume by laser-induced fluorescence and Fraunhofer diffraction combined to mass spectrometry: influence of size and composition of droplets on charge-state distributions of electrosprayed proteins</title><author>GIROD, Marion ; DAGANY, Xavier ; STAFFORD, George ; BOUTOU, Véronique ; BROYER, Michel ; ANTOINE, Rodolphe ; DUGOURD, Philippe ; MORDEHAI, Alex ; LOVE, Craig ; WERLICH, Mark ; FJELDSTED, John</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-2d550df9edbdb470103890dc83372eb2c204dccb747dbc558f0bde95811889e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Chemical Sciences</topic><topic>Chemistry</topic><topic>Cytochromes c - chemistry</topic><topic>Diffraction</topic><topic>Droplets</topic><topic>Evaporation</topic><topic>Exact sciences and technology</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes - chemistry</topic><topic>General and physical chemistry</topic><topic>Lasers</topic><topic>Mass Spectrometry</topic><topic>Methanol - chemistry</topic><topic>Methyl alcohol</topic><topic>Particle Size</topic><topic>Plumes</topic><topic>Profiling</topic><topic>Proteins - chemistry</topic><topic>Solvents - chemistry</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Volatile Organic Compounds - chemistry</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GIROD, Marion</creatorcontrib><creatorcontrib>DAGANY, Xavier</creatorcontrib><creatorcontrib>STAFFORD, George</creatorcontrib><creatorcontrib>BOUTOU, Véronique</creatorcontrib><creatorcontrib>BROYER, Michel</creatorcontrib><creatorcontrib>ANTOINE, Rodolphe</creatorcontrib><creatorcontrib>DUGOURD, Philippe</creatorcontrib><creatorcontrib>MORDEHAI, Alex</creatorcontrib><creatorcontrib>LOVE, Craig</creatorcontrib><creatorcontrib>WERLICH, Mark</creatorcontrib><creatorcontrib>FJELDSTED, John</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Physical chemistry chemical physics : PCCP</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GIROD, Marion</au><au>DAGANY, Xavier</au><au>STAFFORD, George</au><au>BOUTOU, Véronique</au><au>BROYER, Michel</au><au>ANTOINE, Rodolphe</au><au>DUGOURD, Philippe</au><au>MORDEHAI, Alex</au><au>LOVE, Craig</au><au>WERLICH, Mark</au><au>FJELDSTED, John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Profiling an electrospray plume by laser-induced fluorescence and Fraunhofer diffraction combined to mass spectrometry: influence of size and composition of droplets on charge-state distributions of electrosprayed proteins</atitle><jtitle>Physical chemistry chemical physics : PCCP</jtitle><addtitle>Phys Chem Chem Phys</addtitle><date>2012-07-14</date><risdate>2012</risdate><volume>14</volume><issue>26</issue><spage>9389</spage><epage>9396</epage><pages>9389-9396</pages><issn>1463-9076</issn><eissn>1463-9084</eissn><abstract>We investigated how physico-chemical properties of charged droplets are affected by the electrospray process, using simultaneous in situ measurements by laser-induced fluorescence (LIF), Fraunhofer diffraction and mass spectrometry. For this purpose, we implemented a laser-induced-fluorescence profiling setup in conjunction with a fast, high-resolution particle sizing scheme on a modified Agilent Jet Stream electrospray source coupled to a single quadrupole mass analyser. The optical setup permits us to profile the solvent fractionation and the size of the droplets as they evaporate in an electrospray plume by measuring both the angular scattering pattern and emission spectra of a solvatochromic fluorescent dye. Mass spectra are recorded simultaneously. These mass spectrometry and optical spectroscopy investigations allow us to study the relation between the observed charge-state distributions of protein anions and physico-chemical properties of evaporating droplets in the spray plume. By mixing water with methanol, a refolding of cytochrome C is observed as the water percentage increases in the plume due to the preponderant evaporation of volatile methanol.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><pmid>22246045</pmid><doi>10.1039/c2cp23341j</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-9715-7404</orcidid><orcidid>https://orcid.org/0000-0003-4395-1140</orcidid><orcidid>https://orcid.org/0000-0001-5682-8550</orcidid></addata></record> |
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| identifier | ISSN: 1463-9076 |
| ispartof | Physical chemistry chemical physics : PCCP, 2012-07, Vol.14 (26), p.9389-9396 |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Chemical Sciences Chemistry Cytochromes c - chemistry Diffraction Droplets Evaporation Exact sciences and technology Fluorescence Fluorescent Dyes - chemistry General and physical chemistry Lasers Mass Spectrometry Methanol - chemistry Methyl alcohol Particle Size Plumes Profiling Proteins - chemistry Solvents - chemistry Spectrometry, Mass, Electrospray Ionization Volatile Organic Compounds - chemistry Water - chemistry |
| title | Profiling an electrospray plume by laser-induced fluorescence and Fraunhofer diffraction combined to mass spectrometry: influence of size and composition of droplets on charge-state distributions of electrosprayed proteins |
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