Two-photon microscopy of dermal innervation in a human re-innervated model of skin
When skin is injured, innervation can be severely disrupted. The subsequent re‐innervation processes are poorly understood notably because of the inability to image the full meandering course of nerves with their ramifications and endings from histological slices. In this letter, we report on two‐ph...
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| Veröffentlicht in: | Experimental dermatology 2013-04, Vol.22 (4), p.290-291 |
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| creator | Sevrain, David Le Grand, Yann Buhé, Virginie Jeanmaire, Christine Pauly, Gilles Carré, Jean-Luc Misery, Laurent Lebonvallet, Nicolas |
| description | When skin is injured, innervation can be severely disrupted. The subsequent re‐innervation processes are poorly understood notably because of the inability to image the full meandering course of nerves with their ramifications and endings from histological slices. In this letter, we report on two‐photon excitation fluorescence (TPEF) microscopy of entire human skin explants re‐innervated by rodent sensory neurons labelled with the styryl dye FM1‐43. TPEF imaging of nerve fibres to a depth up to roughly 300 μm within the dermis was demonstrated, allowing three‐dimensional reconstruction of the neural tree structure. Endogenous second‐harmonic imaging of type I fibrillar collagen was performed in parallel to TPEF imaging using the same nonlinear microscope, revealing the path of the nerves through the dermis. |
| doi_str_mv | 10.1111/exd.12108 |
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The subsequent re‐innervation processes are poorly understood notably because of the inability to image the full meandering course of nerves with their ramifications and endings from histological slices. In this letter, we report on two‐photon excitation fluorescence (TPEF) microscopy of entire human skin explants re‐innervated by rodent sensory neurons labelled with the styryl dye FM1‐43. TPEF imaging of nerve fibres to a depth up to roughly 300 μm within the dermis was demonstrated, allowing three‐dimensional reconstruction of the neural tree structure. Endogenous second‐harmonic imaging of type I fibrillar collagen was performed in parallel to TPEF imaging using the same nonlinear microscope, revealing the path of the nerves through the dermis.</description><identifier>ISSN: 0906-6705</identifier><identifier>EISSN: 1600-0625</identifier><identifier>DOI: 10.1111/exd.12108</identifier><identifier>PMID: 23445261</identifier><language>eng</language><publisher>Denmark: Blackwell Publishing Ltd</publisher><subject>Animals ; Cellular Biology ; Coculture Techniques ; Dermis ; Dermis - innervation ; Fluorescent Dyes ; FM1-43 ; Humans ; Imaging, Three-Dimensional ; Life Sciences ; Microscopy, Fluorescence, Multiphoton ; Models, Neurological ; Nerve Regeneration ; neuron ; Optics ; Physics ; Pyridinium Compounds ; Quaternary Ammonium Compounds ; Rats ; Rats, Wistar ; re-innervation ; Sensory Receptor Cells ; Sensory Receptor Cells - physiology ; Skin ; Skin - injuries ; Skin - innervation ; styryl dye ; two-photon microscopy</subject><ispartof>Experimental dermatology, 2013-04, Vol.22 (4), p.290-291</ispartof><rights>2013 John Wiley & Sons A/S</rights><rights>2013 John Wiley & Sons A/S.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4328-a3b452fc0e17075f299dc48fe597b471670aea331200c84f80eaf5e27551772a3</citedby><cites>FETCH-LOGICAL-c4328-a3b452fc0e17075f299dc48fe597b471670aea331200c84f80eaf5e27551772a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fexd.12108$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fexd.12108$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,777,781,882,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23445261$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.univ-brest.fr/hal-00947022$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Sevrain, David</creatorcontrib><creatorcontrib>Le Grand, Yann</creatorcontrib><creatorcontrib>Buhé, Virginie</creatorcontrib><creatorcontrib>Jeanmaire, Christine</creatorcontrib><creatorcontrib>Pauly, Gilles</creatorcontrib><creatorcontrib>Carré, Jean-Luc</creatorcontrib><creatorcontrib>Misery, Laurent</creatorcontrib><creatorcontrib>Lebonvallet, Nicolas</creatorcontrib><title>Two-photon microscopy of dermal innervation in a human re-innervated model of skin</title><title>Experimental dermatology</title><addtitle>Exp Dermatol</addtitle><description>When skin is injured, innervation can be severely disrupted. The subsequent re‐innervation processes are poorly understood notably because of the inability to image the full meandering course of nerves with their ramifications and endings from histological slices. In this letter, we report on two‐photon excitation fluorescence (TPEF) microscopy of entire human skin explants re‐innervated by rodent sensory neurons labelled with the styryl dye FM1‐43. TPEF imaging of nerve fibres to a depth up to roughly 300 μm within the dermis was demonstrated, allowing three‐dimensional reconstruction of the neural tree structure. Endogenous second‐harmonic imaging of type I fibrillar collagen was performed in parallel to TPEF imaging using the same nonlinear microscope, revealing the path of the nerves through the dermis.</description><subject>Animals</subject><subject>Cellular Biology</subject><subject>Coculture Techniques</subject><subject>Dermis</subject><subject>Dermis - innervation</subject><subject>Fluorescent Dyes</subject><subject>FM1-43</subject><subject>Humans</subject><subject>Imaging, Three-Dimensional</subject><subject>Life Sciences</subject><subject>Microscopy, Fluorescence, Multiphoton</subject><subject>Models, Neurological</subject><subject>Nerve Regeneration</subject><subject>neuron</subject><subject>Optics</subject><subject>Physics</subject><subject>Pyridinium Compounds</subject><subject>Quaternary Ammonium Compounds</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>re-innervation</subject><subject>Sensory Receptor Cells</subject><subject>Sensory Receptor Cells - physiology</subject><subject>Skin</subject><subject>Skin - injuries</subject><subject>Skin - innervation</subject><subject>styryl dye</subject><subject>two-photon microscopy</subject><issn>0906-6705</issn><issn>1600-0625</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctOwzAQRS0EgvJY8AMoS1gExnYcJ0tEgSJVIKAIdpabTFRDEhe7Bfr3OJSWFd5Y8px7NLom5JDCKQ3nDL_KU8ooZBukR1OAGFImNkkPckjjVILYIbvevwJQyaXYJjuMJ4lgKe2Rh9GnjacTO7Nt1JjCWV_Y6SKyVVSia3QdmbZF96FnJgCmjXQ0mTe6jRzGqwmWUWNLrLuQfzPtPtmqdO3x4PfeI09Xl6OLQTy8u765OB_GRcJZFms-DjtUBSCVIEXF8rwskqxCkctxImnYW6PmnDKAIkuqDFBXApkUgkrJNN8jJ0vvRNdq6kyj3UJZbdTgfKi6N4A8kcDYBw3s8ZKdOvs-Rz9TjfEF1rVu0c69opyFcoTM5Z-2K8M7rNZuCqqrW4W61U_dgT361c7HDZZrctVvAM6WwKepcfG_SV2-9FfKeJkwfoZf64R2byrtfk89316rwf3jw0t_xNQt_wYsQJbQ</recordid><startdate>201304</startdate><enddate>201304</enddate><creator>Sevrain, David</creator><creator>Le Grand, Yann</creator><creator>Buhé, Virginie</creator><creator>Jeanmaire, Christine</creator><creator>Pauly, Gilles</creator><creator>Carré, Jean-Luc</creator><creator>Misery, Laurent</creator><creator>Lebonvallet, Nicolas</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>201304</creationdate><title>Two-photon microscopy of dermal innervation in a human re-innervated model of skin</title><author>Sevrain, David ; Le Grand, Yann ; Buhé, Virginie ; Jeanmaire, Christine ; Pauly, Gilles ; Carré, Jean-Luc ; Misery, Laurent ; Lebonvallet, Nicolas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4328-a3b452fc0e17075f299dc48fe597b471670aea331200c84f80eaf5e27551772a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Cellular Biology</topic><topic>Coculture Techniques</topic><topic>Dermis</topic><topic>Dermis - innervation</topic><topic>Fluorescent Dyes</topic><topic>FM1-43</topic><topic>Humans</topic><topic>Imaging, Three-Dimensional</topic><topic>Life Sciences</topic><topic>Microscopy, Fluorescence, Multiphoton</topic><topic>Models, Neurological</topic><topic>Nerve Regeneration</topic><topic>neuron</topic><topic>Optics</topic><topic>Physics</topic><topic>Pyridinium Compounds</topic><topic>Quaternary Ammonium Compounds</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>re-innervation</topic><topic>Sensory Receptor Cells</topic><topic>Sensory Receptor Cells - physiology</topic><topic>Skin</topic><topic>Skin - injuries</topic><topic>Skin - innervation</topic><topic>styryl dye</topic><topic>two-photon microscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sevrain, David</creatorcontrib><creatorcontrib>Le Grand, Yann</creatorcontrib><creatorcontrib>Buhé, Virginie</creatorcontrib><creatorcontrib>Jeanmaire, Christine</creatorcontrib><creatorcontrib>Pauly, Gilles</creatorcontrib><creatorcontrib>Carré, Jean-Luc</creatorcontrib><creatorcontrib>Misery, Laurent</creatorcontrib><creatorcontrib>Lebonvallet, Nicolas</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Experimental dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sevrain, David</au><au>Le Grand, Yann</au><au>Buhé, Virginie</au><au>Jeanmaire, Christine</au><au>Pauly, Gilles</au><au>Carré, Jean-Luc</au><au>Misery, Laurent</au><au>Lebonvallet, Nicolas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two-photon microscopy of dermal innervation in a human re-innervated model of skin</atitle><jtitle>Experimental dermatology</jtitle><addtitle>Exp Dermatol</addtitle><date>2013-04</date><risdate>2013</risdate><volume>22</volume><issue>4</issue><spage>290</spage><epage>291</epage><pages>290-291</pages><issn>0906-6705</issn><eissn>1600-0625</eissn><abstract>When skin is injured, innervation can be severely disrupted. The subsequent re‐innervation processes are poorly understood notably because of the inability to image the full meandering course of nerves with their ramifications and endings from histological slices. In this letter, we report on two‐photon excitation fluorescence (TPEF) microscopy of entire human skin explants re‐innervated by rodent sensory neurons labelled with the styryl dye FM1‐43. TPEF imaging of nerve fibres to a depth up to roughly 300 μm within the dermis was demonstrated, allowing three‐dimensional reconstruction of the neural tree structure. Endogenous second‐harmonic imaging of type I fibrillar collagen was performed in parallel to TPEF imaging using the same nonlinear microscope, revealing the path of the nerves through the dermis.</abstract><cop>Denmark</cop><pub>Blackwell Publishing Ltd</pub><pmid>23445261</pmid><doi>10.1111/exd.12108</doi><tpages>2</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Cellular Biology Coculture Techniques Dermis Dermis - innervation Fluorescent Dyes FM1-43 Humans Imaging, Three-Dimensional Life Sciences Microscopy, Fluorescence, Multiphoton Models, Neurological Nerve Regeneration neuron Optics Physics Pyridinium Compounds Quaternary Ammonium Compounds Rats Rats, Wistar re-innervation Sensory Receptor Cells Sensory Receptor Cells - physiology Skin Skin - injuries Skin - innervation styryl dye two-photon microscopy |
| title | Two-photon microscopy of dermal innervation in a human re-innervated model of skin |
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