Activation of primary sensory neurons by the topical application of capsaicin on the epidermis of a re-innervated organotypic human skin model
Using an ex vivo skin‐nerve preparation, skin and nerve cells were reconstituted into a single unit and maintained in a nutrient medium bath until required experimentally. Our objective was to use the epidermis as a relay for the induction of an electric current to the neurons following the topical...
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| Veröffentlicht in: | Experimental dermatology 2014-01, Vol.23 (1), p.73-75 |
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| container_title | Experimental dermatology |
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| creator | Lebonvallet, Nicolas Pennec, Jean-Pierre Le Gall-Ianotto, Christelle Chéret, Jérémy Jeanmaire, Christine Carré, Jean-Luc Pauly, Gilles Misery, Laurent |
| description | Using an ex vivo skin‐nerve preparation, skin and nerve cells were reconstituted into a single unit and maintained in a nutrient medium bath until required experimentally. Our objective was to use the epidermis as a relay for the induction of an electric current to the neurons following the topical application of capsaicin on the skin epidermis of the skin explant, an agonist of the TRPV1 channel implicated in pruritus and pain. After 10–20 days of coculture to form the re‐innervated skin model, we applied a solution of capsaicin directly on the epidermis of the skin explant (4 μm). The resulting current was recorded using a path‐clamp technique on the neuronal fibres. Following the topical application of capsaicin, spontaneous activity was triggered, as characterised by repetitive spikes with periods of 125, 225 or 275 ms. This study demonstrates that the skin explant and nerve cells preparation may receive stimuli and be used to screen molecules or to study signal transmission. |
| doi_str_mv | 10.1111/exd.12294 |
| format | Article |
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Our objective was to use the epidermis as a relay for the induction of an electric current to the neurons following the topical application of capsaicin on the skin epidermis of the skin explant, an agonist of the TRPV1 channel implicated in pruritus and pain. After 10–20 days of coculture to form the re‐innervated skin model, we applied a solution of capsaicin directly on the epidermis of the skin explant (4 μm). The resulting current was recorded using a path‐clamp technique on the neuronal fibres. Following the topical application of capsaicin, spontaneous activity was triggered, as characterised by repetitive spikes with periods of 125, 225 or 275 ms. This study demonstrates that the skin explant and nerve cells preparation may receive stimuli and be used to screen molecules or to study signal transmission.</description><identifier>ISSN: 0906-6705</identifier><identifier>EISSN: 1600-0625</identifier><identifier>DOI: 10.1111/exd.12294</identifier><identifier>PMID: 24299199</identifier><language>eng</language><publisher>Denmark: Blackwell Publishing Ltd</publisher><subject>Administration, Topical ; Animals ; Capsaicin - administration & dosage ; Coculture Techniques ; Epidermis - drug effects ; Epidermis - innervation ; human ; Humans ; Life Sciences ; Models, Neurological ; Neurobiology ; neuron ; Neurons and Cognition ; Organ Culture Techniques ; Rats ; Sensory Receptor Cells - drug effects ; Sensory Receptor Cells - physiology ; skin</subject><ispartof>Experimental dermatology, 2014-01, Vol.23 (1), p.73-75</ispartof><rights>2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2013 John Wiley & Sons A/S. 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Our objective was to use the epidermis as a relay for the induction of an electric current to the neurons following the topical application of capsaicin on the skin epidermis of the skin explant, an agonist of the TRPV1 channel implicated in pruritus and pain. After 10–20 days of coculture to form the re‐innervated skin model, we applied a solution of capsaicin directly on the epidermis of the skin explant (4 μm). The resulting current was recorded using a path‐clamp technique on the neuronal fibres. Following the topical application of capsaicin, spontaneous activity was triggered, as characterised by repetitive spikes with periods of 125, 225 or 275 ms. This study demonstrates that the skin explant and nerve cells preparation may receive stimuli and be used to screen molecules or to study signal transmission.</description><subject>Administration, Topical</subject><subject>Animals</subject><subject>Capsaicin - administration & dosage</subject><subject>Coculture Techniques</subject><subject>Epidermis - drug effects</subject><subject>Epidermis - innervation</subject><subject>human</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Models, Neurological</subject><subject>Neurobiology</subject><subject>neuron</subject><subject>Neurons and Cognition</subject><subject>Organ Culture Techniques</subject><subject>Rats</subject><subject>Sensory Receptor Cells - drug effects</subject><subject>Sensory Receptor Cells - physiology</subject><subject>skin</subject><issn>0906-6705</issn><issn>1600-0625</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1u1DAUhS0EokPLghdAXsIire3ETrwcDaVTMZRNEewsx7lhTBM7tZPSeQmeuZ7ODyu8ub72Od_V1UHoHSXnNJ0LeGzOKWOyeIFmVBCSEcH4SzQjkohMlISfoDcx_iaElnnJX6MTVjApqZQz9HduRvugR-sd9i0egu112OAILvpUHUzBu4jrDR7XgEc_WKM7rIehS5eDy-ghamtsatyzDgbbQOht3P5qHCCzzkFIc6DBPvzSzo-bhMLrqdcOx7tk7X0D3Rl61eouwtt9PUXfP1_eLpbZ6tvV9WK-ykzB8iJrmMm5rESdc1IZaOsmr1kttSCiafO84ow2QgrOKylbKgUxdaGppJXgFSmrIj9FH3fcte7UfmnltVXL-Upt3wiRRUkIe6BJ-2GnHYK_nyCOKi1moOu0Az9FRQuZmCJN_Yc1wccYoD2yKVHbqFSKSj1HlbTv99ip7qE5Kg_ZJMHFTvDHdrD5P0ld_vx0QGY7h40jPB4dOtwpsU1e_bi5Ul8XX5bFDedqkT8B0rOtPQ</recordid><startdate>201401</startdate><enddate>201401</enddate><creator>Lebonvallet, Nicolas</creator><creator>Pennec, Jean-Pierre</creator><creator>Le Gall-Ianotto, Christelle</creator><creator>Chéret, Jérémy</creator><creator>Jeanmaire, Christine</creator><creator>Carré, Jean-Luc</creator><creator>Pauly, Gilles</creator><creator>Misery, Laurent</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-0158-2850</orcidid><orcidid>https://orcid.org/0000-0003-1901-0551</orcidid></search><sort><creationdate>201401</creationdate><title>Activation of primary sensory neurons by the topical application of capsaicin on the epidermis of a re-innervated organotypic human skin model</title><author>Lebonvallet, Nicolas ; Pennec, Jean-Pierre ; Le Gall-Ianotto, Christelle ; Chéret, Jérémy ; Jeanmaire, Christine ; Carré, Jean-Luc ; Pauly, Gilles ; Misery, Laurent</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4234-d2c35986b3508cefbd3b2b9a606df338521d69655899f1960cb4a191865807843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Administration, Topical</topic><topic>Animals</topic><topic>Capsaicin - administration & dosage</topic><topic>Coculture Techniques</topic><topic>Epidermis - drug effects</topic><topic>Epidermis - innervation</topic><topic>human</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Models, Neurological</topic><topic>Neurobiology</topic><topic>neuron</topic><topic>Neurons and Cognition</topic><topic>Organ Culture Techniques</topic><topic>Rats</topic><topic>Sensory Receptor Cells - drug effects</topic><topic>Sensory Receptor Cells - physiology</topic><topic>skin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lebonvallet, Nicolas</creatorcontrib><creatorcontrib>Pennec, Jean-Pierre</creatorcontrib><creatorcontrib>Le Gall-Ianotto, Christelle</creatorcontrib><creatorcontrib>Chéret, Jérémy</creatorcontrib><creatorcontrib>Jeanmaire, Christine</creatorcontrib><creatorcontrib>Carré, Jean-Luc</creatorcontrib><creatorcontrib>Pauly, Gilles</creatorcontrib><creatorcontrib>Misery, Laurent</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Experimental dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lebonvallet, Nicolas</au><au>Pennec, Jean-Pierre</au><au>Le Gall-Ianotto, Christelle</au><au>Chéret, Jérémy</au><au>Jeanmaire, Christine</au><au>Carré, Jean-Luc</au><au>Pauly, Gilles</au><au>Misery, Laurent</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activation of primary sensory neurons by the topical application of capsaicin on the epidermis of a re-innervated organotypic human skin model</atitle><jtitle>Experimental dermatology</jtitle><addtitle>Exp Dermatol</addtitle><date>2014-01</date><risdate>2014</risdate><volume>23</volume><issue>1</issue><spage>73</spage><epage>75</epage><pages>73-75</pages><issn>0906-6705</issn><eissn>1600-0625</eissn><abstract>Using an ex vivo skin‐nerve preparation, skin and nerve cells were reconstituted into a single unit and maintained in a nutrient medium bath until required experimentally. Our objective was to use the epidermis as a relay for the induction of an electric current to the neurons following the topical application of capsaicin on the skin epidermis of the skin explant, an agonist of the TRPV1 channel implicated in pruritus and pain. After 10–20 days of coculture to form the re‐innervated skin model, we applied a solution of capsaicin directly on the epidermis of the skin explant (4 μm). The resulting current was recorded using a path‐clamp technique on the neuronal fibres. Following the topical application of capsaicin, spontaneous activity was triggered, as characterised by repetitive spikes with periods of 125, 225 or 275 ms. 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| ispartof | Experimental dermatology, 2014-01, Vol.23 (1), p.73-75 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Administration, Topical Animals Capsaicin - administration & dosage Coculture Techniques Epidermis - drug effects Epidermis - innervation human Humans Life Sciences Models, Neurological Neurobiology neuron Neurons and Cognition Organ Culture Techniques Rats Sensory Receptor Cells - drug effects Sensory Receptor Cells - physiology skin |
| title | Activation of primary sensory neurons by the topical application of capsaicin on the epidermis of a re-innervated organotypic human skin model |
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