Comparison of genome segments 2, 7 and 10 of bluetongue viruses serotype 2 for differentiation between field isolates and the vaccine strain
Bluetongue (BT) virus serotype 2 (BTV 2) was first confirmed in Tunisia in February 2000 and has since spread northward and westward, infecting several other countries and islands, including Corsica, where clinical disease was reported in October 2000. BT was again reported on the Island in July 200...
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| Veröffentlicht in: | Veterinary research (Paris) 2003-11, Vol.34 (6), p.777-789 |
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| creator | BREARD, Emmanuel SAILLEAU, Corinne COUPIER, Hervé MURE-RAVAUD, Karine HAMMOUMI, Saliha GICQUEL, Bernard HAMBLIN, Chris DUBOURGET, Philippe ZIENTARA, Stéphan |
| description | Bluetongue (BT) virus serotype 2 (BTV 2) was first confirmed in Tunisia in February 2000 and has since spread northward and westward, infecting several other countries and islands, including Corsica, where clinical disease was reported in October 2000. BT was again reported on the Island in July 2001, some six months after a vaccination campaign against BTV 2. The molecular relationship between isolates of the BTV 2 Corsican wild-type viruses from 2000 and 2001, and the attenuated BTV 2 vaccine were determined by comparing corresponding sequences of genome segments 2, 7 and 10 with each other and with already published sequences available in the genome database. Complete genetic stability was observed between the isolates of the Corsican BTV 2. There was some divergence between the nucleotide sequences of segment 10 obtained from the wild-type and vaccine virus strains. Based on these differences, primers were selected that could be used in RT-PCR to differentiate between the wild-type and the vaccine viruses. |
| doi_str_mv | 10.1051/vetres:2003036 |
| format | Article |
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BT was again reported on the Island in July 2001, some six months after a vaccination campaign against BTV 2. The molecular relationship between isolates of the BTV 2 Corsican wild-type viruses from 2000 and 2001, and the attenuated BTV 2 vaccine were determined by comparing corresponding sequences of genome segments 2, 7 and 10 with each other and with already published sequences available in the genome database. Complete genetic stability was observed between the isolates of the Corsican BTV 2. There was some divergence between the nucleotide sequences of segment 10 obtained from the wild-type and vaccine virus strains. Based on these differences, primers were selected that could be used in RT-PCR to differentiate between the wild-type and the vaccine viruses.</description><identifier>ISSN: 0928-4249</identifier><identifier>EISSN: 1297-9716</identifier><identifier>DOI: 10.1051/vetres:2003036</identifier><identifier>PMID: 14746772</identifier><language>eng</language><publisher>Les Ulis: EDP Sciences</publisher><subject>Amino Acid Sequence ; Animal biology ; Animal genetics ; Animals ; Applied microbiology ; Base Sequence ; Biochemistry, Molecular Biology ; Biological and medical sciences ; Bluetongue - diagnosis ; Bluetongue - epidemiology ; Bluetongue - prevention & control ; Bluetongue - virology ; Bluetongue virus - genetics ; Bluetongue virus - immunology ; Cell Behavior ; Cellular Biology ; Chick Embryo ; Fundamental and applied biological sciences. Psychology ; Genetic Markers ; Genetic Variation ; Genetics ; Immunology ; Life Sciences ; Microbiology ; Microbiology and Parasitology ; Miscellaneous ; Molecular biology ; Molecular Sequence Data ; Neurons and Cognition ; Neutralization Tests - veterinary ; Reverse Transcriptase Polymerase Chain Reaction - methods ; Reverse Transcriptase Polymerase Chain Reaction - veterinary ; RNA, Viral - analysis ; RNA, Viral - chemistry ; Santé publique et épidémiologie ; Sequence Alignment - veterinary ; Serotyping - veterinary ; Sheep ; Vaccination - veterinary ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Viral Vaccines - genetics ; Virology</subject><ispartof>Veterinary research (Paris), 2003-11, Vol.34 (6), p.777-789</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-6d697507b159eea1007e2e84b23c12e2cfc40a6b0896f67519de107a6e3958bf3</citedby><orcidid>0000-0002-0512-0779 ; 0000-0002-6733-8503</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15378602$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14746772$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00902778$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>BREARD, Emmanuel</creatorcontrib><creatorcontrib>SAILLEAU, Corinne</creatorcontrib><creatorcontrib>COUPIER, Hervé</creatorcontrib><creatorcontrib>MURE-RAVAUD, Karine</creatorcontrib><creatorcontrib>HAMMOUMI, Saliha</creatorcontrib><creatorcontrib>GICQUEL, Bernard</creatorcontrib><creatorcontrib>HAMBLIN, Chris</creatorcontrib><creatorcontrib>DUBOURGET, Philippe</creatorcontrib><creatorcontrib>ZIENTARA, Stéphan</creatorcontrib><title>Comparison of genome segments 2, 7 and 10 of bluetongue viruses serotype 2 for differentiation between field isolates and the vaccine strain</title><title>Veterinary research (Paris)</title><addtitle>Vet Res</addtitle><description>Bluetongue (BT) virus serotype 2 (BTV 2) was first confirmed in Tunisia in February 2000 and has since spread northward and westward, infecting several other countries and islands, including Corsica, where clinical disease was reported in October 2000. BT was again reported on the Island in July 2001, some six months after a vaccination campaign against BTV 2. The molecular relationship between isolates of the BTV 2 Corsican wild-type viruses from 2000 and 2001, and the attenuated BTV 2 vaccine were determined by comparing corresponding sequences of genome segments 2, 7 and 10 with each other and with already published sequences available in the genome database. Complete genetic stability was observed between the isolates of the Corsican BTV 2. There was some divergence between the nucleotide sequences of segment 10 obtained from the wild-type and vaccine virus strains. Based on these differences, primers were selected that could be used in RT-PCR to differentiate between the wild-type and the vaccine viruses.</description><subject>Amino Acid Sequence</subject><subject>Animal biology</subject><subject>Animal genetics</subject><subject>Animals</subject><subject>Applied microbiology</subject><subject>Base Sequence</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biological and medical sciences</subject><subject>Bluetongue - diagnosis</subject><subject>Bluetongue - epidemiology</subject><subject>Bluetongue - prevention & control</subject><subject>Bluetongue - virology</subject><subject>Bluetongue virus - genetics</subject><subject>Bluetongue virus - immunology</subject><subject>Cell Behavior</subject><subject>Cellular Biology</subject><subject>Chick Embryo</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Markers</subject><subject>Genetic Variation</subject><subject>Genetics</subject><subject>Immunology</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Microbiology and Parasitology</subject><subject>Miscellaneous</subject><subject>Molecular biology</subject><subject>Molecular Sequence Data</subject><subject>Neurons and Cognition</subject><subject>Neutralization Tests - veterinary</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - veterinary</subject><subject>RNA, Viral - analysis</subject><subject>RNA, Viral - chemistry</subject><subject>Santé publique et épidémiologie</subject><subject>Sequence Alignment - veterinary</subject><subject>Serotyping - veterinary</subject><subject>Sheep</subject><subject>Vaccination - veterinary</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Viral Vaccines - genetics</subject><subject>Virology</subject><issn>0928-4249</issn><issn>1297-9716</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUGL1DAUx4Mo7uzq1aPkorBg15c0TZq9LYO6woAXPZc0fZmNtM2YpCP7HfzQZpjiHj09yPv9fy_wJ-QNgxsGDft4xBwx3XKAGmr5jGwY16rSisnnZAOat5XgQl-Qy5R-AjBZN-IluWBCCakU35A_2zAdTPQpzDQ4usc5TEgT7iecc6L8A1XUzANlcFr344I5zPsF6dHHJWEqaAz58YCUUxciHbxzGEvWm-yLs8f8G3GmzuM40HJmNLmkTsr8UCzGWj-XgzkaP78iL5wZE75e5xX58fnT9-19tfv25ev2bldZwWWu5CC1akD1rNGIhgEo5NiKnteWceTWWQFG9tBq6aRqmB6QgTISa920vauvyPXZ-2DG7hD9ZOJjF4zv7u923ekNQANXqj2ywr4_s4cYfi2Ycjf5ZHEczYxhSZ1iDeeKt_8FOYNaCC0LeHMGbQwpRXT_vsCgO5XanUvt1lJL4O1qXvoJhyd8bbEA71bAJGtGF81sfXrimlq1Enj9F6Heq0A</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>BREARD, Emmanuel</creator><creator>SAILLEAU, Corinne</creator><creator>COUPIER, Hervé</creator><creator>MURE-RAVAUD, Karine</creator><creator>HAMMOUMI, Saliha</creator><creator>GICQUEL, Bernard</creator><creator>HAMBLIN, Chris</creator><creator>DUBOURGET, Philippe</creator><creator>ZIENTARA, Stéphan</creator><general>EDP Sciences</general><general>BioMed Central</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0002-0512-0779</orcidid><orcidid>https://orcid.org/0000-0002-6733-8503</orcidid></search><sort><creationdate>20031101</creationdate><title>Comparison of genome segments 2, 7 and 10 of bluetongue viruses serotype 2 for differentiation between field isolates and the vaccine strain</title><author>BREARD, Emmanuel ; SAILLEAU, Corinne ; COUPIER, Hervé ; MURE-RAVAUD, Karine ; HAMMOUMI, Saliha ; GICQUEL, Bernard ; HAMBLIN, Chris ; DUBOURGET, Philippe ; ZIENTARA, Stéphan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-6d697507b159eea1007e2e84b23c12e2cfc40a6b0896f67519de107a6e3958bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Amino Acid Sequence</topic><topic>Animal biology</topic><topic>Animal genetics</topic><topic>Animals</topic><topic>Applied microbiology</topic><topic>Base Sequence</topic><topic>Biochemistry, Molecular Biology</topic><topic>Biological and medical sciences</topic><topic>Bluetongue - diagnosis</topic><topic>Bluetongue - epidemiology</topic><topic>Bluetongue - prevention & control</topic><topic>Bluetongue - virology</topic><topic>Bluetongue virus - genetics</topic><topic>Bluetongue virus - immunology</topic><topic>Cell Behavior</topic><topic>Cellular Biology</topic><topic>Chick Embryo</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Markers</topic><topic>Genetic Variation</topic><topic>Genetics</topic><topic>Immunology</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Microbiology and Parasitology</topic><topic>Miscellaneous</topic><topic>Molecular biology</topic><topic>Molecular Sequence Data</topic><topic>Neurons and Cognition</topic><topic>Neutralization Tests - veterinary</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - veterinary</topic><topic>RNA, Viral - analysis</topic><topic>RNA, Viral - chemistry</topic><topic>Santé publique et épidémiologie</topic><topic>Sequence Alignment - veterinary</topic><topic>Serotyping - veterinary</topic><topic>Sheep</topic><topic>Vaccination - veterinary</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Viral Vaccines - genetics</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BREARD, Emmanuel</creatorcontrib><creatorcontrib>SAILLEAU, Corinne</creatorcontrib><creatorcontrib>COUPIER, Hervé</creatorcontrib><creatorcontrib>MURE-RAVAUD, Karine</creatorcontrib><creatorcontrib>HAMMOUMI, Saliha</creatorcontrib><creatorcontrib>GICQUEL, Bernard</creatorcontrib><creatorcontrib>HAMBLIN, Chris</creatorcontrib><creatorcontrib>DUBOURGET, Philippe</creatorcontrib><creatorcontrib>ZIENTARA, Stéphan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Veterinary research (Paris)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BREARD, Emmanuel</au><au>SAILLEAU, Corinne</au><au>COUPIER, Hervé</au><au>MURE-RAVAUD, Karine</au><au>HAMMOUMI, Saliha</au><au>GICQUEL, Bernard</au><au>HAMBLIN, Chris</au><au>DUBOURGET, Philippe</au><au>ZIENTARA, Stéphan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of genome segments 2, 7 and 10 of bluetongue viruses serotype 2 for differentiation between field isolates and the vaccine strain</atitle><jtitle>Veterinary research (Paris)</jtitle><addtitle>Vet Res</addtitle><date>2003-11-01</date><risdate>2003</risdate><volume>34</volume><issue>6</issue><spage>777</spage><epage>789</epage><pages>777-789</pages><issn>0928-4249</issn><eissn>1297-9716</eissn><abstract>Bluetongue (BT) virus serotype 2 (BTV 2) was first confirmed in Tunisia in February 2000 and has since spread northward and westward, infecting several other countries and islands, including Corsica, where clinical disease was reported in October 2000. BT was again reported on the Island in July 2001, some six months after a vaccination campaign against BTV 2. The molecular relationship between isolates of the BTV 2 Corsican wild-type viruses from 2000 and 2001, and the attenuated BTV 2 vaccine were determined by comparing corresponding sequences of genome segments 2, 7 and 10 with each other and with already published sequences available in the genome database. Complete genetic stability was observed between the isolates of the Corsican BTV 2. There was some divergence between the nucleotide sequences of segment 10 obtained from the wild-type and vaccine virus strains. Based on these differences, primers were selected that could be used in RT-PCR to differentiate between the wild-type and the vaccine viruses.</abstract><cop>Les Ulis</cop><pub>EDP Sciences</pub><pmid>14746772</pmid><doi>10.1051/vetres:2003036</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-0512-0779</orcidid><orcidid>https://orcid.org/0000-0002-6733-8503</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Veterinary research (Paris), 2003-11, Vol.34 (6), p.777-789 |
| issn | 0928-4249 1297-9716 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amino Acid Sequence Animal biology Animal genetics Animals Applied microbiology Base Sequence Biochemistry, Molecular Biology Biological and medical sciences Bluetongue - diagnosis Bluetongue - epidemiology Bluetongue - prevention & control Bluetongue - virology Bluetongue virus - genetics Bluetongue virus - immunology Cell Behavior Cellular Biology Chick Embryo Fundamental and applied biological sciences. Psychology Genetic Markers Genetic Variation Genetics Immunology Life Sciences Microbiology Microbiology and Parasitology Miscellaneous Molecular biology Molecular Sequence Data Neurons and Cognition Neutralization Tests - veterinary Reverse Transcriptase Polymerase Chain Reaction - methods Reverse Transcriptase Polymerase Chain Reaction - veterinary RNA, Viral - analysis RNA, Viral - chemistry Santé publique et épidémiologie Sequence Alignment - veterinary Serotyping - veterinary Sheep Vaccination - veterinary Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Viral Vaccines - genetics Virology |
| title | Comparison of genome segments 2, 7 and 10 of bluetongue viruses serotype 2 for differentiation between field isolates and the vaccine strain |
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