Proteic boost enhances humoral response induced by DNA vaccination with the dnaK gene of Chlamydophila abortus but fails to protect pregnant mice against a virulence challenge
In order to enhance the quantity and the protective properties of the antibodies induced by DNA vaccination with the heat shock protein dnaK gene of Chlamydophila abortus AB7 as well as to elicit an efficient cellular immune response, we vaccinated mice with a DNA prime followed by a boost with the...
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| Veröffentlicht in: | Veterinary research (Paris) 2003-01, Vol.34 (1), p.119-125 |
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| creator | HECHARD, Céline GREPINET, Olivier RODOLAKIS, Annie |
| description | In order to enhance the quantity and the protective properties of the antibodies induced by DNA vaccination with the heat shock protein dnaK gene of Chlamydophila abortus AB7 as well as to elicit an efficient cellular immune response, we vaccinated mice with a DNA prime followed by a boost with the recombinant DnaK protein. In non-pregnant mice, this strategy induced the same predominance of the IgG2a isotype as DNA immunization alone with a substantial increased antibody level. The induced antibodies had no in vitro neutralizing properties on C. abortus infectivity. Moreover, the proteic boost probably failed to elicit an efficient cellular immune response since the pregnant or non-pregnant mice were not protected against the bacterial challenge. |
| doi_str_mv | 10.1051/vetres:2002052 |
| format | Article |
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In non-pregnant mice, this strategy induced the same predominance of the IgG2a isotype as DNA immunization alone with a substantial increased antibody level. The induced antibodies had no in vitro neutralizing properties on C. abortus infectivity. Moreover, the proteic boost probably failed to elicit an efficient cellular immune response since the pregnant or non-pregnant mice were not protected against the bacterial challenge.</description><identifier>ISSN: 0928-4249</identifier><identifier>EISSN: 1297-9716</identifier><identifier>DOI: 10.1051/vetres:2002052</identifier><identifier>PMID: 12588688</identifier><language>eng</language><publisher>Les Ulis: EDP Sciences</publisher><subject>Animal biology ; Animal genetics ; Animals ; Antibodies, Bacterial - immunology ; Bacterial Vaccines - genetics ; Bacterial Vaccines - immunology ; Bacteriology ; Biochemistry, Molecular Biology ; Biological and medical sciences ; Cell Behavior ; Cellular Biology ; Chlamydophila - genetics ; Chlamydophila - immunology ; Chlamydophila - pathogenicity ; Chlamydophila abortus ; Chlamydophila Infections - immunology ; Chlamydophila Infections - prevention & control ; dnaK gene ; Escherichia coli Proteins ; Female ; Fundamental and applied biological sciences. Psychology ; Genetics ; HSP70 Heat-Shock Proteins - genetics ; HSP70 Heat-Shock Proteins - immunology ; Immunoglobulin G - immunology ; Immunology ; Life Sciences ; Mice ; Microbiology ; Microbiology and Parasitology ; Molecular biology ; Neurons and Cognition ; Pregnancy ; Santé publique et épidémiologie ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccines, DNA - genetics ; Vaccines, DNA - immunology ; Virulence</subject><ispartof>Veterinary research (Paris), 2003-01, Vol.34 (1), p.119-125</ispartof><rights>2003 INIST-CNRS</rights><rights>Copyright</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-46c8b1d3295dcb2cd8a7883cd4f5413b7c3859ca483cdb1c5087b3d0d70a43db3</citedby><orcidid>0000-0001-5085-474X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14709215$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12588688$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00902611$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>HECHARD, Céline</creatorcontrib><creatorcontrib>GREPINET, Olivier</creatorcontrib><creatorcontrib>RODOLAKIS, Annie</creatorcontrib><title>Proteic boost enhances humoral response induced by DNA vaccination with the dnaK gene of Chlamydophila abortus but fails to protect pregnant mice against a virulence challenge</title><title>Veterinary research (Paris)</title><addtitle>Vet Res</addtitle><description>In order to enhance the quantity and the protective properties of the antibodies induced by DNA vaccination with the heat shock protein dnaK gene of Chlamydophila abortus AB7 as well as to elicit an efficient cellular immune response, we vaccinated mice with a DNA prime followed by a boost with the recombinant DnaK protein. In non-pregnant mice, this strategy induced the same predominance of the IgG2a isotype as DNA immunization alone with a substantial increased antibody level. The induced antibodies had no in vitro neutralizing properties on C. abortus infectivity. Moreover, the proteic boost probably failed to elicit an efficient cellular immune response since the pregnant or non-pregnant mice were not protected against the bacterial challenge.</description><subject>Animal biology</subject><subject>Animal genetics</subject><subject>Animals</subject><subject>Antibodies, Bacterial - immunology</subject><subject>Bacterial Vaccines - genetics</subject><subject>Bacterial Vaccines - immunology</subject><subject>Bacteriology</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biological and medical sciences</subject><subject>Cell Behavior</subject><subject>Cellular Biology</subject><subject>Chlamydophila - genetics</subject><subject>Chlamydophila - immunology</subject><subject>Chlamydophila - pathogenicity</subject><subject>Chlamydophila abortus</subject><subject>Chlamydophila Infections - immunology</subject><subject>Chlamydophila Infections - prevention & control</subject><subject>dnaK gene</subject><subject>Escherichia coli Proteins</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>HSP70 Heat-Shock Proteins - genetics</subject><subject>HSP70 Heat-Shock Proteins - immunology</subject><subject>Immunoglobulin G - immunology</subject><subject>Immunology</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Microbiology and Parasitology</subject><subject>Molecular biology</subject><subject>Neurons and Cognition</subject><subject>Pregnancy</subject><subject>Santé publique et épidémiologie</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccines, DNA - genetics</subject><subject>Vaccines, DNA - immunology</subject><subject>Virulence</subject><issn>0928-4249</issn><issn>1297-9716</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk-P0zAQxS0EYrsLV45oLiBxyGI7ceJwqwrLIirgAOdo_CeNUWKX2Cnqp-Ir4qoVe-Q0o9Fv3jzrmZAXjN4yKtjbg02zje84pZwK_oisGG-bom1Y_ZisaMtlUfGqvSLXMf6klNWlqJ6SK8aFlLWUK_Ln2xySdRpUCDGB9QN6bSMMyxRmHCGL74OPFpw3i7YG1BHef1nDAbV2HpMLHn67NEAaLBiPn2FnvYXQw2YYcTqasB_ciIAqzGmJoJYEPboxQgqwP93WKVe78-gTTE5bwB06n70gHNy8jDb7AT3gmLudfUae9DhG-_xSb8iPuw_fN_fF9uvHT5v1ttAVr1NR1VoqZkreCqMV10ZiI2WpTdWLipWq0aUUrcbqNFNMCyobVRpqGopVaVR5Q96cdfPhbj-7CedjF9B19-ttd5pR2lJeM3ZgmX19ZvN7fi02pm5yUdtxRG_DErumpFxm9L8gk62gopUZvD2Deg4xzrb_Z4HR7pR7d869u-SeF15elBc1WfOAX4LOwKsLgFHj2M85ZhcfuKrJn4WJ8i9MdroB</recordid><startdate>20030101</startdate><enddate>20030101</enddate><creator>HECHARD, Céline</creator><creator>GREPINET, Olivier</creator><creator>RODOLAKIS, Annie</creator><general>EDP Sciences</general><general>BioMed Central</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0001-5085-474X</orcidid></search><sort><creationdate>20030101</creationdate><title>Proteic boost enhances humoral response induced by DNA vaccination with the dnaK gene of Chlamydophila abortus but fails to protect pregnant mice against a virulence challenge</title><author>HECHARD, Céline ; GREPINET, Olivier ; RODOLAKIS, Annie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-46c8b1d3295dcb2cd8a7883cd4f5413b7c3859ca483cdb1c5087b3d0d70a43db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animal biology</topic><topic>Animal genetics</topic><topic>Animals</topic><topic>Antibodies, Bacterial - immunology</topic><topic>Bacterial Vaccines - genetics</topic><topic>Bacterial Vaccines - immunology</topic><topic>Bacteriology</topic><topic>Biochemistry, Molecular Biology</topic><topic>Biological and medical sciences</topic><topic>Cell Behavior</topic><topic>Cellular Biology</topic><topic>Chlamydophila - genetics</topic><topic>Chlamydophila - immunology</topic><topic>Chlamydophila - pathogenicity</topic><topic>Chlamydophila abortus</topic><topic>Chlamydophila Infections - immunology</topic><topic>Chlamydophila Infections - prevention & control</topic><topic>dnaK gene</topic><topic>Escherichia coli Proteins</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>HSP70 Heat-Shock Proteins - genetics</topic><topic>HSP70 Heat-Shock Proteins - immunology</topic><topic>Immunoglobulin G - immunology</topic><topic>Immunology</topic><topic>Life Sciences</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Microbiology and Parasitology</topic><topic>Molecular biology</topic><topic>Neurons and Cognition</topic><topic>Pregnancy</topic><topic>Santé publique et épidémiologie</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vaccines, DNA - genetics</topic><topic>Vaccines, DNA - immunology</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HECHARD, Céline</creatorcontrib><creatorcontrib>GREPINET, Olivier</creatorcontrib><creatorcontrib>RODOLAKIS, Annie</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Veterinary research (Paris)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HECHARD, Céline</au><au>GREPINET, Olivier</au><au>RODOLAKIS, Annie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteic boost enhances humoral response induced by DNA vaccination with the dnaK gene of Chlamydophila abortus but fails to protect pregnant mice against a virulence challenge</atitle><jtitle>Veterinary research (Paris)</jtitle><addtitle>Vet Res</addtitle><date>2003-01-01</date><risdate>2003</risdate><volume>34</volume><issue>1</issue><spage>119</spage><epage>125</epage><pages>119-125</pages><issn>0928-4249</issn><eissn>1297-9716</eissn><abstract>In order to enhance the quantity and the protective properties of the antibodies induced by DNA vaccination with the heat shock protein dnaK gene of Chlamydophila abortus AB7 as well as to elicit an efficient cellular immune response, we vaccinated mice with a DNA prime followed by a boost with the recombinant DnaK protein. In non-pregnant mice, this strategy induced the same predominance of the IgG2a isotype as DNA immunization alone with a substantial increased antibody level. The induced antibodies had no in vitro neutralizing properties on C. abortus infectivity. Moreover, the proteic boost probably failed to elicit an efficient cellular immune response since the pregnant or non-pregnant mice were not protected against the bacterial challenge.</abstract><cop>Les Ulis</cop><pub>EDP Sciences</pub><pmid>12588688</pmid><doi>10.1051/vetres:2002052</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-5085-474X</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Veterinary research (Paris), 2003-01, Vol.34 (1), p.119-125 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animal biology Animal genetics Animals Antibodies, Bacterial - immunology Bacterial Vaccines - genetics Bacterial Vaccines - immunology Bacteriology Biochemistry, Molecular Biology Biological and medical sciences Cell Behavior Cellular Biology Chlamydophila - genetics Chlamydophila - immunology Chlamydophila - pathogenicity Chlamydophila abortus Chlamydophila Infections - immunology Chlamydophila Infections - prevention & control dnaK gene Escherichia coli Proteins Female Fundamental and applied biological sciences. Psychology Genetics HSP70 Heat-Shock Proteins - genetics HSP70 Heat-Shock Proteins - immunology Immunoglobulin G - immunology Immunology Life Sciences Mice Microbiology Microbiology and Parasitology Molecular biology Neurons and Cognition Pregnancy Santé publique et épidémiologie Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, DNA - genetics Vaccines, DNA - immunology Virulence |
| title | Proteic boost enhances humoral response induced by DNA vaccination with the dnaK gene of Chlamydophila abortus but fails to protect pregnant mice against a virulence challenge |
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