Probing the Plasma Membrane Organization in Living Cells by Spot Variation Fluorescence Correlation Spectroscopy

While intrinsic Brownian agitation within a lipid bilayer does homogenize the molecular distribution, the extremely diverse composition of the plasma membrane, in contrast, favors the development of inhomogeneity due to the propensity of such a system to minimize its total free energy. Precisely, de...

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Veröffentlicht in:	Methods in Enzymology 2013, Vol.519, p.277-302
Hauptverfasser:	Billaudeau, Cyrille, Mailfert, Sébastien, Trombik, Tomasz, Bertaux, Nicolas, Rouger, Vincent, Hamon, Yannick, He, Hai-Tao, Marguet, Didier
Format:	Artikel
Sprache:	eng
Schlagworte:	Calibration Cell Adhesion Cell Line Cell membranes Computer Science Confinement Engineering Sciences Fluorescence Fluorescence correlation spectroscopy Humans Lateral diffusion Microscopy - methods Nanodomains Plasma membrane Signal and Image processing Spectrometry, Fluorescence - methods Spectroscopy
Online-Zugang:	Volltext
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