Cellular localization, accumulation and trafficking of double-walled carbon nanotubes in human prostate cancer cells
Carbon nanotubes (CNTs) are at present being considered as potential nanovectors with the ability to deliver therapeutic cargoes into living cells. Previous studies established the ability of CNTs to enter cells and their therapeutic utility, but an appreciation of global intracellular trafficking a...
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| Veröffentlicht in: | Nano research 2012-04, Vol.5 (4), p.223-234 |
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| creator | Neves, Vera Gerondopoulos, Andreas Heister, Elena Tîlmaciu, Carmen Flahaut, Emmanuel Soula, B. Silva, S. Ravi P. McFadden, Johnjoe Coley, Helen M. |
| description | Carbon nanotubes (CNTs) are at present being considered as potential nanovectors with the ability to deliver therapeutic cargoes into living cells. Previous studies established the ability of CNTs to enter cells and their therapeutic utility, but an appreciation of global intracellular trafficking associated with their cellular distribution has yet to be described. Despite the many aspects of the uptake mechanism of CNTs being studied, only a few studies have investigated internalization and fate of CNTs inside cells in detail. In the present study, intracellular localization and trafficking of RNA-wrapped, oxidized double-walled CNTs (oxDWNT-RNA) is presented. Fixed cells, previously exposed to oxDWNT-RNA, were subjected to immunocytochemical analysis using antibodies specific to proteins implicated in endocytosis; moreover cell compartment markers and pharmacological inhibitory conditions were also employed in this study. Our results revealed that an endocytic pathway is involved in the internalization of oxDWNT-RNA. The nanotubes were found in clathrin-coated vesicles, after which they appear to be sorted in early endosomes, followed by vesicular maturation, become located in lysosomes. Furthermore, we observed co-localization of oxDWNT-RNA with the small GTP-binding protein (Rab 11), involved in their recycling back to the plasma membrane via endosomes from the trans-golgi network. |
| doi_str_mv | 10.1007/s12274-012-0202-9 |
| format | Article |
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Fixed cells, previously exposed to oxDWNT-RNA, were subjected to immunocytochemical analysis using antibodies specific to proteins implicated in endocytosis; moreover cell compartment markers and pharmacological inhibitory conditions were also employed in this study. Our results revealed that an endocytic pathway is involved in the internalization of oxDWNT-RNA. The nanotubes were found in clathrin-coated vesicles, after which they appear to be sorted in early endosomes, followed by vesicular maturation, become located in lysosomes. Furthermore, we observed co-localization of oxDWNT-RNA with the small GTP-binding protein (Rab 11), involved in their recycling back to the plasma membrane via endosomes from the trans-golgi network.</description><identifier>ISSN: 1998-0124</identifier><identifier>EISSN: 1998-0000</identifier><identifier>DOI: 10.1007/s12274-012-0202-9</identifier><language>eng</language><publisher>Heidelberg: Tsinghua Press</publisher><subject>Atomic/Molecular Structure and Spectra ; Biomedicine ; Biotechnology ; Cancer ; Carbon ; Carbon nanotubes ; Cellular ; Chemistry and Materials Science ; Condensed Matter Physics ; Electrons ; Endosomes ; Engineering Sciences ; Life Sciences ; Localization ; Materials ; Materials Science ; Nanocomposites ; Nanomaterials ; Nanostructure ; Nanotechnology ; Plasma ; Position (location) ; Prostate cancer ; Proteins ; Research Article</subject><ispartof>Nano research, 2012-04, Vol.5 (4), p.223-234</ispartof><rights>Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2012</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-e484d17d55f9c2c67718653985973017b3ee9976f0574fa4a1bfeb7ab97adc053</citedby><cites>FETCH-LOGICAL-c482t-e484d17d55f9c2c67718653985973017b3ee9976f0574fa4a1bfeb7ab97adc053</cites><orcidid>0000-0001-8344-6902</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12274-012-0202-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12274-012-0202-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,777,781,882,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://hal.science/hal-00836017$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Neves, Vera</creatorcontrib><creatorcontrib>Gerondopoulos, Andreas</creatorcontrib><creatorcontrib>Heister, Elena</creatorcontrib><creatorcontrib>Tîlmaciu, Carmen</creatorcontrib><creatorcontrib>Flahaut, Emmanuel</creatorcontrib><creatorcontrib>Soula, B.</creatorcontrib><creatorcontrib>Silva, S. Ravi P.</creatorcontrib><creatorcontrib>McFadden, Johnjoe</creatorcontrib><creatorcontrib>Coley, Helen M.</creatorcontrib><title>Cellular localization, accumulation and trafficking of double-walled carbon nanotubes in human prostate cancer cells</title><title>Nano research</title><addtitle>Nano Res</addtitle><description>Carbon nanotubes (CNTs) are at present being considered as potential nanovectors with the ability to deliver therapeutic cargoes into living cells. Previous studies established the ability of CNTs to enter cells and their therapeutic utility, but an appreciation of global intracellular trafficking associated with their cellular distribution has yet to be described. Despite the many aspects of the uptake mechanism of CNTs being studied, only a few studies have investigated internalization and fate of CNTs inside cells in detail. In the present study, intracellular localization and trafficking of RNA-wrapped, oxidized double-walled CNTs (oxDWNT-RNA) is presented. Fixed cells, previously exposed to oxDWNT-RNA, were subjected to immunocytochemical analysis using antibodies specific to proteins implicated in endocytosis; moreover cell compartment markers and pharmacological inhibitory conditions were also employed in this study. Our results revealed that an endocytic pathway is involved in the internalization of oxDWNT-RNA. The nanotubes were found in clathrin-coated vesicles, after which they appear to be sorted in early endosomes, followed by vesicular maturation, become located in lysosomes. 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Ravi P.</au><au>McFadden, Johnjoe</au><au>Coley, Helen M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellular localization, accumulation and trafficking of double-walled carbon nanotubes in human prostate cancer cells</atitle><jtitle>Nano research</jtitle><stitle>Nano Res</stitle><date>2012-04-01</date><risdate>2012</risdate><volume>5</volume><issue>4</issue><spage>223</spage><epage>234</epage><pages>223-234</pages><issn>1998-0124</issn><eissn>1998-0000</eissn><abstract>Carbon nanotubes (CNTs) are at present being considered as potential nanovectors with the ability to deliver therapeutic cargoes into living cells. Previous studies established the ability of CNTs to enter cells and their therapeutic utility, but an appreciation of global intracellular trafficking associated with their cellular distribution has yet to be described. 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| identifier | ISSN: 1998-0124 |
| ispartof | Nano research, 2012-04, Vol.5 (4), p.223-234 |
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| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Atomic/Molecular Structure and Spectra Biomedicine Biotechnology Cancer Carbon Carbon nanotubes Cellular Chemistry and Materials Science Condensed Matter Physics Electrons Endosomes Engineering Sciences Life Sciences Localization Materials Materials Science Nanocomposites Nanomaterials Nanostructure Nanotechnology Plasma Position (location) Prostate cancer Proteins Research Article |
| title | Cellular localization, accumulation and trafficking of double-walled carbon nanotubes in human prostate cancer cells |
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