Activation of bovine neutrophil oxidase in a cell free system. GTP-dependent formation of a complex between a cytosolic factor and a membrane protein

The effect of guanine nucleotides on activation of the O 2 ⨪ generating oxidase in a cell free system consisting of bovine neutrophils membranes, cytosol and arachidonic acid has been studied. In a complete system, GTP-γ-S was stimulatory and GDP-β-S inhibitory. When cytosol was omitted, both nucleo...

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Veröffentlicht in:	Biochemical and biophysical research communications 1988-05, Vol.152 (3), p.993-1001
Hauptverfasser:	Doussiere, Jacques, Pilloud, Marie-Claire, Vignais, Pierre V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Arachidonic Acid Arachidonic Acids Arachidonic Acids - pharmacology Biological and medical sciences Cattle Cell-Free System Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Glutaral Glutaral - pharmacology Guanosine 5'-O-(3-Thiotriphosphate) Guanosine Diphosphate Guanosine Diphosphate - analogs & derivatives Guanosine Diphosphate - pharmacology Guanosine Triphosphate Guanosine Triphosphate - analogs & derivatives Guanosine Triphosphate - metabolism Guanosine Triphosphate - pharmacology Immunology Innate immunity Life Sciences Membrane Proteins Membrane Proteins - metabolism NADH, NADPH Oxidoreductases NADH, NADPH Oxidoreductases - blood NADPH Oxidase NADPH Oxidases Neutrophils Neutrophils - enzymology Oxidoreductases Thionucleotides Thionucleotides - pharmacology
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