Role of Prenylation in the Interaction of Rho-Family Small GTPases with GTPase Activating Proteins

The role of prenylation in the interaction of Rho-family small GTPases with their GTPase activating proteins (GAPs) was investigated. Prenylated and nonprenylated small GTPases were expressed in Sf9 insect cells and Escherichia coli, respectively. Nucleotide binding to and hydrolysis by prenylated a...

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Veröffentlicht in:	Biochemistry (Easton) 2001-09, Vol.40 (35), p.10542-10549
Hauptverfasser:	Molnár, Gergely, Dagher, Marie-Claire, Geiszt, Miklós, Settleman, Jeffrey, Ligeti, Erzsébet
Format:	Artikel
Sprache:	eng
Schlagworte:	Enzyme Activation Fluorides Fluorides - pharmacology GTP Phosphohydrolases GTP Phosphohydrolases - metabolism GTPase-Activating Proteins GTPase-Activating Proteins - metabolism Humans Immunology In Vitro Techniques Innate immunity Life Sciences Membrane Proteins Membrane Proteins - metabolism Neutrophils Neutrophils - metabolism Protein Prenylation rac GTP-Binding Proteins rac GTP-Binding Proteins - metabolism Recombinant Proteins Recombinant Proteins - metabolism rho GTP-Binding Proteins rho GTP-Binding Proteins - metabolism Subcellular Fractions
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