Kinetics of enzymatic digestion of feeds as estimated by a stepwise in vitro method
Nutritional feed values are currently based on aggregate criteria such as the ileal or faecal digestibility. Digestibility is the result of several processes including hydrolysis, absorption, secretion and passage. In order to develop mechanistic models of digestion to be used for feed evaluation, t...
Gespeichert in:
| Veröffentlicht in: | Animal feed science and technology 2008-03, Vol.141 (1), p.171-183 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 183 |
|---|---|
| container_issue | 1 |
| container_start_page | 171 |
| container_title | Animal feed science and technology |
| container_volume | 141 |
| creator | Wilfart, A. Jaguelin-Peyraud, Y. Simmins, H. Noblet, J. van Milgen, J. Montagne, L. |
| description | Nutritional feed values are currently based on aggregate criteria such as the ileal or faecal digestibility. Digestibility is the result of several processes including hydrolysis, absorption, secretion and passage. In order to develop mechanistic models of digestion to be used for feed evaluation, these processes have to be quantified. The aim of the current study was to determine the enzymatic hydrolysis (or:
in vitro digestion) kinetics of main constituents (organic matter, nitrogen and starch) in wheat, barley, wheat bran and soybean meal, using a three-step, enzymatic
in vitro method that mimics digestion in the stomach, small and large intestine of pigs. The
in vitro results were compared with
in vivo results. Hydrolysis kinetics (
i.e., solubilisation of feed constituents) was modelled using an exponential segmented model estimating the extent and rate of digestion for each enzymatic digestion step.
In vitro digestion of organic matter of soybean meal occurred mainly through the action of pepsin (0.67
versus |
| doi_str_mv | 10.1016/j.anifeedsci.2007.05.021 |
| format | Article |
| fullrecord | <record><control><sourceid>hal_cross</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_00729874v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0377840107001800</els_id><sourcerecordid>oai_HAL_hal_00729874v1</sourcerecordid><originalsourceid>FETCH-LOGICAL-c406t-3c070baac31083a7035e20eff3cc23f34642c395074d5c41f261b0f209989d183</originalsourceid><addsrcrecordid>eNqFkT1PwzAQhi0EEuXjN-CFgSHhbCexM5aKL1GJoXS2XOcMrkpS2VFR-fU4BMHIZPnuec-nx4RQBjkDVl2vc9N6h9hE63MOIHMoc-DsgEyYkiLjnFWHZAJCykwVwI7JSYxrAMaV4BOyePIt9t5G2jmK7ef-3aQbbfwrxt537VD-nk5NpEMp9bGhqz01NPa4_fARqW_pzveho-_Yv3XNGTlyZhPx_Oc8Jcu725fZQzZ_vn-cTeeZLaDqM2FBwsoYKxgoYSSIEjmgc8JaLpwoqoJbUZcgi6a0BXO8YitwHOpa1Q1T4pRcjXPfzEZvQ1ot7HVnvH6YzvVQSzZ4rWSxY4lVI2tDF2NA9xtgoAeReq3_ROpBpIZSJ5EpejlGtyZas3HBtNbH3zwHxhRUReIuRs6ZTpvXkJjlInUFgJLpL-pE3IwEJis7j0Gnt7C12PiAttdN5_9f5ws1uJYX</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Kinetics of enzymatic digestion of feeds as estimated by a stepwise in vitro method</title><source>Elsevier ScienceDirect Journals Complete</source><creator>Wilfart, A. ; Jaguelin-Peyraud, Y. ; Simmins, H. ; Noblet, J. ; van Milgen, J. ; Montagne, L.</creator><creatorcontrib>Wilfart, A. ; Jaguelin-Peyraud, Y. ; Simmins, H. ; Noblet, J. ; van Milgen, J. ; Montagne, L.</creatorcontrib><description>Nutritional feed values are currently based on aggregate criteria such as the ileal or faecal digestibility. Digestibility is the result of several processes including hydrolysis, absorption, secretion and passage. In order to develop mechanistic models of digestion to be used for feed evaluation, these processes have to be quantified. The aim of the current study was to determine the enzymatic hydrolysis (or:
in vitro digestion) kinetics of main constituents (organic matter, nitrogen and starch) in wheat, barley, wheat bran and soybean meal, using a three-step, enzymatic
in vitro method that mimics digestion in the stomach, small and large intestine of pigs. The
in vitro results were compared with
in vivo results. Hydrolysis kinetics (
i.e., solubilisation of feed constituents) was modelled using an exponential segmented model estimating the extent and rate of digestion for each enzymatic digestion step.
In vitro digestion of organic matter of soybean meal occurred mainly through the action of pepsin (0.67
versus <0.30 for other feeds, P<0.05), which was caused by the enzymatic digestion of protein at this site. Organic matter of cereals was mainly hydrolysed by enzymes mimicking digestion in the small intestine, and was mainly caused by starch digestion. Fractional
in vitro digestion rates of organic matter were higher in the stomach than in the small intestine for cereals (0.20–0.34
min
−1
versus 0.02–0.15
min
−1, P<0.05). The potential
in vitro digestibility of organic matter was nearly 0.88 for wheat and soybean meal, 0.79 for barley and 0.61 for wheat bran, which correspond to typical
in vivo digestibility values. The
in vitro digestibility corresponded reasonably well to
in vivo results for enzyme systems mimicking ileal and total tract digestion. The results of this study indicate that it is possible to quantify dynamic aspects of digestion of feedstuffs fed to non-ruminant animals.</description><identifier>ISSN: 0377-8401</identifier><identifier>EISSN: 1873-2216</identifier><identifier>DOI: 10.1016/j.anifeedsci.2007.05.021</identifier><identifier>CODEN: AFSTDH</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Agricultural sciences ; Animal production studies ; Animal productions ; Biological and medical sciences ; digestibility ; Digestion ; enzymatic hydrolysis ; estimation ; Feed ; Feed and pet food industries ; feed barley ; feed quality ; Food industries ; forage evaluation ; Fundamental and applied biological sciences. Psychology ; in vitro digestibility ; in vitro digestion ; In vitro method ; kinetics ; Life Sciences ; mechanistic models ; nitrogen ; nutritive value ; organic matter ; Pig ; rumen fermentation ; simulation models ; soybean meal ; starch ; Terrestrial animal productions ; Vertebrates ; wheat ; wheat starch</subject><ispartof>Animal feed science and technology, 2008-03, Vol.141 (1), p.171-183</ispartof><rights>2007 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-3c070baac31083a7035e20eff3cc23f34642c395074d5c41f261b0f209989d183</citedby><cites>FETCH-LOGICAL-c406t-3c070baac31083a7035e20eff3cc23f34642c395074d5c41f261b0f209989d183</cites><orcidid>0000-0002-3593-0334 ; 0000-0002-6131-5255 ; 0000-0002-9540-1872</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.anifeedsci.2007.05.021$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20118064$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://institut-agro-rennes-angers.hal.science/hal-00729874$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Wilfart, A.</creatorcontrib><creatorcontrib>Jaguelin-Peyraud, Y.</creatorcontrib><creatorcontrib>Simmins, H.</creatorcontrib><creatorcontrib>Noblet, J.</creatorcontrib><creatorcontrib>van Milgen, J.</creatorcontrib><creatorcontrib>Montagne, L.</creatorcontrib><title>Kinetics of enzymatic digestion of feeds as estimated by a stepwise in vitro method</title><title>Animal feed science and technology</title><description>Nutritional feed values are currently based on aggregate criteria such as the ileal or faecal digestibility. Digestibility is the result of several processes including hydrolysis, absorption, secretion and passage. In order to develop mechanistic models of digestion to be used for feed evaluation, these processes have to be quantified. The aim of the current study was to determine the enzymatic hydrolysis (or:
in vitro digestion) kinetics of main constituents (organic matter, nitrogen and starch) in wheat, barley, wheat bran and soybean meal, using a three-step, enzymatic
in vitro method that mimics digestion in the stomach, small and large intestine of pigs. The
in vitro results were compared with
in vivo results. Hydrolysis kinetics (
i.e., solubilisation of feed constituents) was modelled using an exponential segmented model estimating the extent and rate of digestion for each enzymatic digestion step.
In vitro digestion of organic matter of soybean meal occurred mainly through the action of pepsin (0.67
versus <0.30 for other feeds, P<0.05), which was caused by the enzymatic digestion of protein at this site. Organic matter of cereals was mainly hydrolysed by enzymes mimicking digestion in the small intestine, and was mainly caused by starch digestion. Fractional
in vitro digestion rates of organic matter were higher in the stomach than in the small intestine for cereals (0.20–0.34
min
−1
versus 0.02–0.15
min
−1, P<0.05). The potential
in vitro digestibility of organic matter was nearly 0.88 for wheat and soybean meal, 0.79 for barley and 0.61 for wheat bran, which correspond to typical
in vivo digestibility values. The
in vitro digestibility corresponded reasonably well to
in vivo results for enzyme systems mimicking ileal and total tract digestion. The results of this study indicate that it is possible to quantify dynamic aspects of digestion of feedstuffs fed to non-ruminant animals.</description><subject>Agricultural sciences</subject><subject>Animal production studies</subject><subject>Animal productions</subject><subject>Biological and medical sciences</subject><subject>digestibility</subject><subject>Digestion</subject><subject>enzymatic hydrolysis</subject><subject>estimation</subject><subject>Feed</subject><subject>Feed and pet food industries</subject><subject>feed barley</subject><subject>feed quality</subject><subject>Food industries</subject><subject>forage evaluation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>in vitro digestibility</subject><subject>in vitro digestion</subject><subject>In vitro method</subject><subject>kinetics</subject><subject>Life Sciences</subject><subject>mechanistic models</subject><subject>nitrogen</subject><subject>nutritive value</subject><subject>organic matter</subject><subject>Pig</subject><subject>rumen fermentation</subject><subject>simulation models</subject><subject>soybean meal</subject><subject>starch</subject><subject>Terrestrial animal productions</subject><subject>Vertebrates</subject><subject>wheat</subject><subject>wheat starch</subject><issn>0377-8401</issn><issn>1873-2216</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkT1PwzAQhi0EEuXjN-CFgSHhbCexM5aKL1GJoXS2XOcMrkpS2VFR-fU4BMHIZPnuec-nx4RQBjkDVl2vc9N6h9hE63MOIHMoc-DsgEyYkiLjnFWHZAJCykwVwI7JSYxrAMaV4BOyePIt9t5G2jmK7ef-3aQbbfwrxt537VD-nk5NpEMp9bGhqz01NPa4_fARqW_pzveho-_Yv3XNGTlyZhPx_Oc8Jcu725fZQzZ_vn-cTeeZLaDqM2FBwsoYKxgoYSSIEjmgc8JaLpwoqoJbUZcgi6a0BXO8YitwHOpa1Q1T4pRcjXPfzEZvQ1ot7HVnvH6YzvVQSzZ4rWSxY4lVI2tDF2NA9xtgoAeReq3_ROpBpIZSJ5EpejlGtyZas3HBtNbH3zwHxhRUReIuRs6ZTpvXkJjlInUFgJLpL-pE3IwEJis7j0Gnt7C12PiAttdN5_9f5ws1uJYX</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Wilfart, A.</creator><creator>Jaguelin-Peyraud, Y.</creator><creator>Simmins, H.</creator><creator>Noblet, J.</creator><creator>van Milgen, J.</creator><creator>Montagne, L.</creator><general>Elsevier B.V</general><general>[New York, NY]: Elsevier Science</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-3593-0334</orcidid><orcidid>https://orcid.org/0000-0002-6131-5255</orcidid><orcidid>https://orcid.org/0000-0002-9540-1872</orcidid></search><sort><creationdate>20080301</creationdate><title>Kinetics of enzymatic digestion of feeds as estimated by a stepwise in vitro method</title><author>Wilfart, A. ; Jaguelin-Peyraud, Y. ; Simmins, H. ; Noblet, J. ; van Milgen, J. ; Montagne, L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-3c070baac31083a7035e20eff3cc23f34642c395074d5c41f261b0f209989d183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Agricultural sciences</topic><topic>Animal production studies</topic><topic>Animal productions</topic><topic>Biological and medical sciences</topic><topic>digestibility</topic><topic>Digestion</topic><topic>enzymatic hydrolysis</topic><topic>estimation</topic><topic>Feed</topic><topic>Feed and pet food industries</topic><topic>feed barley</topic><topic>feed quality</topic><topic>Food industries</topic><topic>forage evaluation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>in vitro digestibility</topic><topic>in vitro digestion</topic><topic>In vitro method</topic><topic>kinetics</topic><topic>Life Sciences</topic><topic>mechanistic models</topic><topic>nitrogen</topic><topic>nutritive value</topic><topic>organic matter</topic><topic>Pig</topic><topic>rumen fermentation</topic><topic>simulation models</topic><topic>soybean meal</topic><topic>starch</topic><topic>Terrestrial animal productions</topic><topic>Vertebrates</topic><topic>wheat</topic><topic>wheat starch</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wilfart, A.</creatorcontrib><creatorcontrib>Jaguelin-Peyraud, Y.</creatorcontrib><creatorcontrib>Simmins, H.</creatorcontrib><creatorcontrib>Noblet, J.</creatorcontrib><creatorcontrib>van Milgen, J.</creatorcontrib><creatorcontrib>Montagne, L.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Animal feed science and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wilfart, A.</au><au>Jaguelin-Peyraud, Y.</au><au>Simmins, H.</au><au>Noblet, J.</au><au>van Milgen, J.</au><au>Montagne, L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kinetics of enzymatic digestion of feeds as estimated by a stepwise in vitro method</atitle><jtitle>Animal feed science and technology</jtitle><date>2008-03-01</date><risdate>2008</risdate><volume>141</volume><issue>1</issue><spage>171</spage><epage>183</epage><pages>171-183</pages><issn>0377-8401</issn><eissn>1873-2216</eissn><coden>AFSTDH</coden><abstract>Nutritional feed values are currently based on aggregate criteria such as the ileal or faecal digestibility. Digestibility is the result of several processes including hydrolysis, absorption, secretion and passage. In order to develop mechanistic models of digestion to be used for feed evaluation, these processes have to be quantified. The aim of the current study was to determine the enzymatic hydrolysis (or:
in vitro digestion) kinetics of main constituents (organic matter, nitrogen and starch) in wheat, barley, wheat bran and soybean meal, using a three-step, enzymatic
in vitro method that mimics digestion in the stomach, small and large intestine of pigs. The
in vitro results were compared with
in vivo results. Hydrolysis kinetics (
i.e., solubilisation of feed constituents) was modelled using an exponential segmented model estimating the extent and rate of digestion for each enzymatic digestion step.
In vitro digestion of organic matter of soybean meal occurred mainly through the action of pepsin (0.67
versus <0.30 for other feeds, P<0.05), which was caused by the enzymatic digestion of protein at this site. Organic matter of cereals was mainly hydrolysed by enzymes mimicking digestion in the small intestine, and was mainly caused by starch digestion. Fractional
in vitro digestion rates of organic matter were higher in the stomach than in the small intestine for cereals (0.20–0.34
min
−1
versus 0.02–0.15
min
−1, P<0.05). The potential
in vitro digestibility of organic matter was nearly 0.88 for wheat and soybean meal, 0.79 for barley and 0.61 for wheat bran, which correspond to typical
in vivo digestibility values. The
in vitro digestibility corresponded reasonably well to
in vivo results for enzyme systems mimicking ileal and total tract digestion. The results of this study indicate that it is possible to quantify dynamic aspects of digestion of feedstuffs fed to non-ruminant animals.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.anifeedsci.2007.05.021</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-3593-0334</orcidid><orcidid>https://orcid.org/0000-0002-6131-5255</orcidid><orcidid>https://orcid.org/0000-0002-9540-1872</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0377-8401 |
| ispartof | Animal feed science and technology, 2008-03, Vol.141 (1), p.171-183 |
| issn | 0377-8401 1873-2216 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_00729874v1 |
| source | Elsevier ScienceDirect Journals Complete |
| subjects | Agricultural sciences Animal production studies Animal productions Biological and medical sciences digestibility Digestion enzymatic hydrolysis estimation Feed Feed and pet food industries feed barley feed quality Food industries forage evaluation Fundamental and applied biological sciences. Psychology in vitro digestibility in vitro digestion In vitro method kinetics Life Sciences mechanistic models nitrogen nutritive value organic matter Pig rumen fermentation simulation models soybean meal starch Terrestrial animal productions Vertebrates wheat wheat starch |
| title | Kinetics of enzymatic digestion of feeds as estimated by a stepwise in vitro method |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T22%3A05%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-hal_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Kinetics%20of%20enzymatic%20digestion%20of%20feeds%20as%20estimated%20by%20a%20stepwise%20in%20vitro%20method&rft.jtitle=Animal%20feed%20science%20and%20technology&rft.au=Wilfart,%20A.&rft.date=2008-03-01&rft.volume=141&rft.issue=1&rft.spage=171&rft.epage=183&rft.pages=171-183&rft.issn=0377-8401&rft.eissn=1873-2216&rft.coden=AFSTDH&rft_id=info:doi/10.1016/j.anifeedsci.2007.05.021&rft_dat=%3Chal_cross%3Eoai_HAL_hal_00729874v1%3C/hal_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_els_id=S0377840107001800&rfr_iscdi=true |