Towards high throughput production of artificial egg oocytes using microfluidics
The production of micron-size droplets using microfluidic tools offers new opportunities to carry out biological assays in a controlled environment. We apply these strategies by using a flow-focusing microfluidic device to encapsulate Xenopus egg extracts, a biological system recapitulating key even...
Gespeichert in:
| Veröffentlicht in: | Lab on a chip 2011-02, Vol.11 (3), p.429-434 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 434 |
|---|---|
| container_issue | 3 |
| container_start_page | 429 |
| container_title | Lab on a chip |
| container_volume | 11 |
| creator | Jimenez, A M Roché, M Pinot, M Panizza, P Courbin, L Gueroui, Z |
| description | The production of micron-size droplets using microfluidic tools offers new opportunities to carry out biological assays in a controlled environment. We apply these strategies by using a flow-focusing microfluidic device to encapsulate Xenopus egg extracts, a biological system recapitulating key events of eukaryotic cell functions in vitro. We present a method to generate monodisperse egg extract-in-oil droplets and use high-speed imaging to characterize the droplet pinch-off dynamics leading to the production of trains of droplets. We use fluorescence microscopy to show that our method does not affect the biological activity of the encapsulated egg extract by observing the self-organization of microtubules and actin filaments, two main biopolymers of the cell cytoskeleton, encapsulated in the produced droplets. We anticipate that this assay might be useful for quantitative studies of biological systems in a confined environment as well as high throughput screenings for drug discovery. |
| doi_str_mv | 10.1039/c0lc00046a |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_00711640v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>845765054</sourcerecordid><originalsourceid>FETCH-LOGICAL-c386t-3dcaba21459e58072392931e2d8149b49713867f6165226769200dfa3f8cd3e13</originalsourceid><addsrcrecordid>eNo9kNFKwzAUhoMobk5vfADJnShUT5o0aS5HUScU9GJelyxN20i7zKZR9vZ2bu7qHA4fP-f_ELom8ECAykcNrQYAxtUJmhImaAQklafHXYoJuvD-E4AkjKfnaBITEDEDMUXvS_ej-tLjxtYNHprehbrZhAFvelcGPVi3xq7Cqh9sZbVVLTZ1jZ3T28F4HLxd17izundVG2xptb9EZ5Vqvbk6zBn6eH5aZosof3t5zeZ5pGnKh4iWWq1UTFgiTZKO31AZS0pMXKaEyRWTgoycqDjhSRxzwWUMUFaKVqkuqSF0hu72uY1qi01vO9VvC6dssZjnxe4GIAjhDL537O2eHUt9BeOHorNem7ZVa-OCL1KWCJ5Awkbyfk-OjbzvTXWMJlDsZBcZ5Nmf7PkI3xxiw6oz5RH9t0t_AWnReHo</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>845765054</pqid></control><display><type>article</type><title>Towards high throughput production of artificial egg oocytes using microfluidics</title><source>MEDLINE</source><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Jimenez, A M ; Roché, M ; Pinot, M ; Panizza, P ; Courbin, L ; Gueroui, Z</creator><creatorcontrib>Jimenez, A M ; Roché, M ; Pinot, M ; Panizza, P ; Courbin, L ; Gueroui, Z</creatorcontrib><description>The production of micron-size droplets using microfluidic tools offers new opportunities to carry out biological assays in a controlled environment. We apply these strategies by using a flow-focusing microfluidic device to encapsulate Xenopus egg extracts, a biological system recapitulating key events of eukaryotic cell functions in vitro. We present a method to generate monodisperse egg extract-in-oil droplets and use high-speed imaging to characterize the droplet pinch-off dynamics leading to the production of trains of droplets. We use fluorescence microscopy to show that our method does not affect the biological activity of the encapsulated egg extract by observing the self-organization of microtubules and actin filaments, two main biopolymers of the cell cytoskeleton, encapsulated in the produced droplets. We anticipate that this assay might be useful for quantitative studies of biological systems in a confined environment as well as high throughput screenings for drug discovery.</description><identifier>ISSN: 1473-0197</identifier><identifier>EISSN: 1473-0189</identifier><identifier>DOI: 10.1039/c0lc00046a</identifier><identifier>PMID: 21072407</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Actin Cytoskeleton ; Actin Cytoskeleton - ultrastructure ; Animals ; Biochemistry, Molecular Biology ; Biophysics ; Biopolymers ; Dimethylpolysiloxanes ; Dimethylpolysiloxanes - chemistry ; Life Sciences ; Linear Models ; Microfluidic Analytical Techniques ; Microfluidic Analytical Techniques - instrumentation ; Microfluidics ; Microfluidics - instrumentation ; Microfluidics - methods ; Microscopy, Fluorescence ; Microtubules ; Microtubules - ultrastructure ; Nylons ; Nylons - chemistry ; Oocytes ; Oocytes - metabolism ; Xenopus laevis</subject><ispartof>Lab on a chip, 2011-02, Vol.11 (3), p.429-434</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-3dcaba21459e58072392931e2d8149b49713867f6165226769200dfa3f8cd3e13</citedby><cites>FETCH-LOGICAL-c386t-3dcaba21459e58072392931e2d8149b49713867f6165226769200dfa3f8cd3e13</cites><orcidid>0000-0003-4738-733X ; 0000-0003-1210-0597 ; 0000-0003-4029-3437</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21072407$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00711640$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Jimenez, A M</creatorcontrib><creatorcontrib>Roché, M</creatorcontrib><creatorcontrib>Pinot, M</creatorcontrib><creatorcontrib>Panizza, P</creatorcontrib><creatorcontrib>Courbin, L</creatorcontrib><creatorcontrib>Gueroui, Z</creatorcontrib><title>Towards high throughput production of artificial egg oocytes using microfluidics</title><title>Lab on a chip</title><addtitle>Lab Chip</addtitle><description>The production of micron-size droplets using microfluidic tools offers new opportunities to carry out biological assays in a controlled environment. We apply these strategies by using a flow-focusing microfluidic device to encapsulate Xenopus egg extracts, a biological system recapitulating key events of eukaryotic cell functions in vitro. We present a method to generate monodisperse egg extract-in-oil droplets and use high-speed imaging to characterize the droplet pinch-off dynamics leading to the production of trains of droplets. We use fluorescence microscopy to show that our method does not affect the biological activity of the encapsulated egg extract by observing the self-organization of microtubules and actin filaments, two main biopolymers of the cell cytoskeleton, encapsulated in the produced droplets. We anticipate that this assay might be useful for quantitative studies of biological systems in a confined environment as well as high throughput screenings for drug discovery.</description><subject>Actin Cytoskeleton</subject><subject>Actin Cytoskeleton - ultrastructure</subject><subject>Animals</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biophysics</subject><subject>Biopolymers</subject><subject>Dimethylpolysiloxanes</subject><subject>Dimethylpolysiloxanes - chemistry</subject><subject>Life Sciences</subject><subject>Linear Models</subject><subject>Microfluidic Analytical Techniques</subject><subject>Microfluidic Analytical Techniques - instrumentation</subject><subject>Microfluidics</subject><subject>Microfluidics - instrumentation</subject><subject>Microfluidics - methods</subject><subject>Microscopy, Fluorescence</subject><subject>Microtubules</subject><subject>Microtubules - ultrastructure</subject><subject>Nylons</subject><subject>Nylons - chemistry</subject><subject>Oocytes</subject><subject>Oocytes - metabolism</subject><subject>Xenopus laevis</subject><issn>1473-0197</issn><issn>1473-0189</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kNFKwzAUhoMobk5vfADJnShUT5o0aS5HUScU9GJelyxN20i7zKZR9vZ2bu7qHA4fP-f_ELom8ECAykcNrQYAxtUJmhImaAQklafHXYoJuvD-E4AkjKfnaBITEDEDMUXvS_ej-tLjxtYNHprehbrZhAFvelcGPVi3xq7Cqh9sZbVVLTZ1jZ3T28F4HLxd17izundVG2xptb9EZ5Vqvbk6zBn6eH5aZosof3t5zeZ5pGnKh4iWWq1UTFgiTZKO31AZS0pMXKaEyRWTgoycqDjhSRxzwWUMUFaKVqkuqSF0hu72uY1qi01vO9VvC6dssZjnxe4GIAjhDL537O2eHUt9BeOHorNem7ZVa-OCL1KWCJ5Awkbyfk-OjbzvTXWMJlDsZBcZ5Nmf7PkI3xxiw6oz5RH9t0t_AWnReHo</recordid><startdate>20110207</startdate><enddate>20110207</enddate><creator>Jimenez, A M</creator><creator>Roché, M</creator><creator>Pinot, M</creator><creator>Panizza, P</creator><creator>Courbin, L</creator><creator>Gueroui, Z</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-4738-733X</orcidid><orcidid>https://orcid.org/0000-0003-1210-0597</orcidid><orcidid>https://orcid.org/0000-0003-4029-3437</orcidid></search><sort><creationdate>20110207</creationdate><title>Towards high throughput production of artificial egg oocytes using microfluidics</title><author>Jimenez, A M ; Roché, M ; Pinot, M ; Panizza, P ; Courbin, L ; Gueroui, Z</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-3dcaba21459e58072392931e2d8149b49713867f6165226769200dfa3f8cd3e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Actin Cytoskeleton</topic><topic>Actin Cytoskeleton - ultrastructure</topic><topic>Animals</topic><topic>Biochemistry, Molecular Biology</topic><topic>Biophysics</topic><topic>Biopolymers</topic><topic>Dimethylpolysiloxanes</topic><topic>Dimethylpolysiloxanes - chemistry</topic><topic>Life Sciences</topic><topic>Linear Models</topic><topic>Microfluidic Analytical Techniques</topic><topic>Microfluidic Analytical Techniques - instrumentation</topic><topic>Microfluidics</topic><topic>Microfluidics - instrumentation</topic><topic>Microfluidics - methods</topic><topic>Microscopy, Fluorescence</topic><topic>Microtubules</topic><topic>Microtubules - ultrastructure</topic><topic>Nylons</topic><topic>Nylons - chemistry</topic><topic>Oocytes</topic><topic>Oocytes - metabolism</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jimenez, A M</creatorcontrib><creatorcontrib>Roché, M</creatorcontrib><creatorcontrib>Pinot, M</creatorcontrib><creatorcontrib>Panizza, P</creatorcontrib><creatorcontrib>Courbin, L</creatorcontrib><creatorcontrib>Gueroui, Z</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Lab on a chip</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jimenez, A M</au><au>Roché, M</au><au>Pinot, M</au><au>Panizza, P</au><au>Courbin, L</au><au>Gueroui, Z</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Towards high throughput production of artificial egg oocytes using microfluidics</atitle><jtitle>Lab on a chip</jtitle><addtitle>Lab Chip</addtitle><date>2011-02-07</date><risdate>2011</risdate><volume>11</volume><issue>3</issue><spage>429</spage><epage>434</epage><pages>429-434</pages><issn>1473-0197</issn><eissn>1473-0189</eissn><abstract>The production of micron-size droplets using microfluidic tools offers new opportunities to carry out biological assays in a controlled environment. We apply these strategies by using a flow-focusing microfluidic device to encapsulate Xenopus egg extracts, a biological system recapitulating key events of eukaryotic cell functions in vitro. We present a method to generate monodisperse egg extract-in-oil droplets and use high-speed imaging to characterize the droplet pinch-off dynamics leading to the production of trains of droplets. We use fluorescence microscopy to show that our method does not affect the biological activity of the encapsulated egg extract by observing the self-organization of microtubules and actin filaments, two main biopolymers of the cell cytoskeleton, encapsulated in the produced droplets. We anticipate that this assay might be useful for quantitative studies of biological systems in a confined environment as well as high throughput screenings for drug discovery.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>21072407</pmid><doi>10.1039/c0lc00046a</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-4738-733X</orcidid><orcidid>https://orcid.org/0000-0003-1210-0597</orcidid><orcidid>https://orcid.org/0000-0003-4029-3437</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1473-0197 |
| ispartof | Lab on a chip, 2011-02, Vol.11 (3), p.429-434 |
| issn | 1473-0197 1473-0189 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_00711640v1 |
| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Actin Cytoskeleton Actin Cytoskeleton - ultrastructure Animals Biochemistry, Molecular Biology Biophysics Biopolymers Dimethylpolysiloxanes Dimethylpolysiloxanes - chemistry Life Sciences Linear Models Microfluidic Analytical Techniques Microfluidic Analytical Techniques - instrumentation Microfluidics Microfluidics - instrumentation Microfluidics - methods Microscopy, Fluorescence Microtubules Microtubules - ultrastructure Nylons Nylons - chemistry Oocytes Oocytes - metabolism Xenopus laevis |
| title | Towards high throughput production of artificial egg oocytes using microfluidics |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T18%3A45%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Towards%20high%20throughput%20production%20of%20artificial%20egg%20oocytes%20using%20microfluidics&rft.jtitle=Lab%20on%20a%20chip&rft.au=Jimenez,%20A%20M&rft.date=2011-02-07&rft.volume=11&rft.issue=3&rft.spage=429&rft.epage=434&rft.pages=429-434&rft.issn=1473-0197&rft.eissn=1473-0189&rft_id=info:doi/10.1039/c0lc00046a&rft_dat=%3Cproquest_hal_p%3E845765054%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=845765054&rft_id=info:pmid/21072407&rfr_iscdi=true |