Impedance spectroscopy and conductometric biosensing for probing catalase reaction with cyanide as ligand and inhibitor
In this work, a new biosensor was prepared through immobilization of bovine liver catalase in a photoreticulated poly (vinyl alcohol) membrane at the surface of a conductometric transducer. This biosensor was used to study the kinetics of catalase–H 20 2 reaction and its inhibition by cyanide. Immob...
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description | In this work, a new biosensor was prepared through immobilization of bovine liver catalase in a photoreticulated poly (vinyl alcohol) membrane at the surface of a conductometric transducer. This biosensor was used to study the kinetics of catalase–H
20
2 reaction and its inhibition by cyanide. Immobilized catalase exhibited a Michaelis–Menten behaviour at low H
20
2 concentrations (<
100
mM) with apparent constant K
M
app
=
84
±
3
mM and maximal initial velocity V
M
app
=
13.4
μS
min
−
1
. Inhibition by cyanide was found to be non-competitive and inhibition binding constant K
i was 13.9
±
0.3
μM. The decrease of the biosensor response by increasing cyanide concentration was linear up to 50
μM, with a cyanide detection limit of 6
μM. In parallel, electrochemical characteristics of the catalase/PVA biomembrane and its interaction with cyanide were studied by cyclic voltammetry and impedance spectroscopy. Addition of the biomembrane onto the gold electrodes induced a significant increase of the interfacial polarization resistance R
P. On the contrary, cyanide binding resulted in a decrease of Rp proportional to KCN concentration in the 4 to 50
μM range. Inhibition coefficient I
50 calculated by this powerful label-free and substrate-free technique (24.3
μM) was in good agreement with that determined from the substrate-dependent conductometric biosensor (24.9
μM). |
doi_str_mv | 10.1016/j.bioelechem.2010.07.006 |
format | Article |
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20
2 reaction and its inhibition by cyanide. Immobilized catalase exhibited a Michaelis–Menten behaviour at low H
20
2 concentrations (<
100
mM) with apparent constant K
M
app
=
84
±
3
mM and maximal initial velocity V
M
app
=
13.4
μS
min
−
1
. Inhibition by cyanide was found to be non-competitive and inhibition binding constant K
i was 13.9
±
0.3
μM. The decrease of the biosensor response by increasing cyanide concentration was linear up to 50
μM, with a cyanide detection limit of 6
μM. In parallel, electrochemical characteristics of the catalase/PVA biomembrane and its interaction with cyanide were studied by cyclic voltammetry and impedance spectroscopy. Addition of the biomembrane onto the gold electrodes induced a significant increase of the interfacial polarization resistance R
P. On the contrary, cyanide binding resulted in a decrease of Rp proportional to KCN concentration in the 4 to 50
μM range. Inhibition coefficient I
50 calculated by this powerful label-free and substrate-free technique (24.3
μM) was in good agreement with that determined from the substrate-dependent conductometric biosensor (24.9
μM).</description><identifier>ISSN: 1567-5394</identifier><identifier>EISSN: 1878-562X</identifier><identifier>DOI: 10.1016/j.bioelechem.2010.07.006</identifier><identifier>PMID: 20813591</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Analytical chemistry ; Animals ; Biochemistry ; Biochemistry, Molecular Biology ; Biosensing Techniques - methods ; Catalase ; Catalase - chemistry ; Catalase - metabolism ; Cattle ; Chemical Sciences ; Conductometric biosensor ; Conductometry - methods ; Cyanide ; Cyanides - chemistry ; Cyanides - metabolism ; Dielectric Spectroscopy - methods ; Electrochemistry - methods ; Electrodes ; Enzyme Inhibitors - chemistry ; Enzyme Inhibitors - metabolism ; Enzymes, Immobilized - chemistry ; Enzymes, Immobilized - metabolism ; Hydrogen Peroxide - metabolism ; I 50 ; Impedimetric biosensor ; Inhibition ; Life Sciences ; Ligands ; Limit of Detection</subject><ispartof>Bioelectrochemistry (Amsterdam, Netherlands), 2011-02, Vol.80 (2), p.155-161</ispartof><rights>2010 Elsevier B.V.</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-fd4a0552b77f9fb94631768d157a13a093b61b6d0cc56f14c286a581f84ed7973</citedby><cites>FETCH-LOGICAL-c439t-fd4a0552b77f9fb94631768d157a13a093b61b6d0cc56f14c286a581f84ed7973</cites><orcidid>0000-0003-1354-9273</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bioelechem.2010.07.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,778,782,883,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20813591$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00703801$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Bouyahia, Naima</creatorcontrib><creatorcontrib>Hamlaoui, Mohamed Larbi</creatorcontrib><creatorcontrib>Hnaien, Mouna</creatorcontrib><creatorcontrib>Lagarde, Florence</creatorcontrib><creatorcontrib>Jaffrezic-Renault, Nicole</creatorcontrib><title>Impedance spectroscopy and conductometric biosensing for probing catalase reaction with cyanide as ligand and inhibitor</title><title>Bioelectrochemistry (Amsterdam, Netherlands)</title><addtitle>Bioelectrochemistry</addtitle><description>In this work, a new biosensor was prepared through immobilization of bovine liver catalase in a photoreticulated poly (vinyl alcohol) membrane at the surface of a conductometric transducer. This biosensor was used to study the kinetics of catalase–H
20
2 reaction and its inhibition by cyanide. Immobilized catalase exhibited a Michaelis–Menten behaviour at low H
20
2 concentrations (<
100
mM) with apparent constant K
M
app
=
84
±
3
mM and maximal initial velocity V
M
app
=
13.4
μS
min
−
1
. Inhibition by cyanide was found to be non-competitive and inhibition binding constant K
i was 13.9
±
0.3
μM. The decrease of the biosensor response by increasing cyanide concentration was linear up to 50
μM, with a cyanide detection limit of 6
μM. In parallel, electrochemical characteristics of the catalase/PVA biomembrane and its interaction with cyanide were studied by cyclic voltammetry and impedance spectroscopy. Addition of the biomembrane onto the gold electrodes induced a significant increase of the interfacial polarization resistance R
P. On the contrary, cyanide binding resulted in a decrease of Rp proportional to KCN concentration in the 4 to 50
μM range. Inhibition coefficient I
50 calculated by this powerful label-free and substrate-free technique (24.3
μM) was in good agreement with that determined from the substrate-dependent conductometric biosensor (24.9
μM).</description><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biosensing Techniques - methods</subject><subject>Catalase</subject><subject>Catalase - chemistry</subject><subject>Catalase - metabolism</subject><subject>Cattle</subject><subject>Chemical Sciences</subject><subject>Conductometric biosensor</subject><subject>Conductometry - methods</subject><subject>Cyanide</subject><subject>Cyanides - chemistry</subject><subject>Cyanides - metabolism</subject><subject>Dielectric Spectroscopy - methods</subject><subject>Electrochemistry - methods</subject><subject>Electrodes</subject><subject>Enzyme Inhibitors - chemistry</subject><subject>Enzyme Inhibitors - metabolism</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Enzymes, Immobilized - metabolism</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>I 50</subject><subject>Impedimetric biosensor</subject><subject>Inhibition</subject><subject>Life Sciences</subject><subject>Ligands</subject><subject>Limit of Detection</subject><issn>1567-5394</issn><issn>1878-562X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEoh_wF5BviEOWcRJ_5FiqQiutxAUkbpZjT7peJXawva323-NoS3vswfJo9My8M_NWFaGwoUD51_1mcAEnNDucNw2UNIgNAH9TnVMpZM148-dtiRkXNWv77qy6SGkPAJIK9r46a0rQsp6eV49384JWe4MkLWhyDMmE5Ui0t8QEbw8mhxlzdIYUyYQ-OX9PxhDJEsOwxkZnPemEJKI22QVPHl3eEXPU3lkkOpHJ3a_t1uf8zg0uh_ihejfqKeHHp_-y-v395tf1bb39-ePu-mpbm67tcz3aTgNjzSDE2I9D3_GWCi4tZULTVkPfDpwO3IIxjI-0M43kmkk6yg6t6EV7WX059d3pSS3RzToeVdBO3V5t1ZoDENBKoA-0sJ9PbFnt7wFTVrNLBqdJewyHpKRkwDro2OtkA6LAYu0pT6Qpl00Rx-chKKjVS7VXL16q1UsFokzFS-mnJ5HDMKN9LvxvXgG-nQAsB3xwGFUyDouV1sVipbLBva7yD5Jitf0</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>Bouyahia, Naima</creator><creator>Hamlaoui, Mohamed Larbi</creator><creator>Hnaien, Mouna</creator><creator>Lagarde, Florence</creator><creator>Jaffrezic-Renault, Nicole</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-1354-9273</orcidid></search><sort><creationdate>20110201</creationdate><title>Impedance spectroscopy and conductometric biosensing for probing catalase reaction with cyanide as ligand and inhibitor</title><author>Bouyahia, Naima ; Hamlaoui, Mohamed Larbi ; Hnaien, Mouna ; Lagarde, Florence ; Jaffrezic-Renault, Nicole</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-fd4a0552b77f9fb94631768d157a13a093b61b6d0cc56f14c286a581f84ed7973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biochemistry, Molecular Biology</topic><topic>Biosensing Techniques - methods</topic><topic>Catalase</topic><topic>Catalase - chemistry</topic><topic>Catalase - metabolism</topic><topic>Cattle</topic><topic>Chemical Sciences</topic><topic>Conductometric biosensor</topic><topic>Conductometry - methods</topic><topic>Cyanide</topic><topic>Cyanides - chemistry</topic><topic>Cyanides - metabolism</topic><topic>Dielectric Spectroscopy - methods</topic><topic>Electrochemistry - methods</topic><topic>Electrodes</topic><topic>Enzyme Inhibitors - chemistry</topic><topic>Enzyme Inhibitors - metabolism</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Enzymes, Immobilized - metabolism</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>I 50</topic><topic>Impedimetric biosensor</topic><topic>Inhibition</topic><topic>Life Sciences</topic><topic>Ligands</topic><topic>Limit of Detection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bouyahia, Naima</creatorcontrib><creatorcontrib>Hamlaoui, Mohamed Larbi</creatorcontrib><creatorcontrib>Hnaien, Mouna</creatorcontrib><creatorcontrib>Lagarde, Florence</creatorcontrib><creatorcontrib>Jaffrezic-Renault, Nicole</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Bioelectrochemistry (Amsterdam, Netherlands)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bouyahia, Naima</au><au>Hamlaoui, Mohamed Larbi</au><au>Hnaien, Mouna</au><au>Lagarde, Florence</au><au>Jaffrezic-Renault, Nicole</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impedance spectroscopy and conductometric biosensing for probing catalase reaction with cyanide as ligand and inhibitor</atitle><jtitle>Bioelectrochemistry (Amsterdam, Netherlands)</jtitle><addtitle>Bioelectrochemistry</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>80</volume><issue>2</issue><spage>155</spage><epage>161</epage><pages>155-161</pages><issn>1567-5394</issn><eissn>1878-562X</eissn><abstract>In this work, a new biosensor was prepared through immobilization of bovine liver catalase in a photoreticulated poly (vinyl alcohol) membrane at the surface of a conductometric transducer. This biosensor was used to study the kinetics of catalase–H
20
2 reaction and its inhibition by cyanide. Immobilized catalase exhibited a Michaelis–Menten behaviour at low H
20
2 concentrations (<
100
mM) with apparent constant K
M
app
=
84
±
3
mM and maximal initial velocity V
M
app
=
13.4
μS
min
−
1
. Inhibition by cyanide was found to be non-competitive and inhibition binding constant K
i was 13.9
±
0.3
μM. The decrease of the biosensor response by increasing cyanide concentration was linear up to 50
μM, with a cyanide detection limit of 6
μM. In parallel, electrochemical characteristics of the catalase/PVA biomembrane and its interaction with cyanide were studied by cyclic voltammetry and impedance spectroscopy. Addition of the biomembrane onto the gold electrodes induced a significant increase of the interfacial polarization resistance R
P. On the contrary, cyanide binding resulted in a decrease of Rp proportional to KCN concentration in the 4 to 50
μM range. Inhibition coefficient I
50 calculated by this powerful label-free and substrate-free technique (24.3
μM) was in good agreement with that determined from the substrate-dependent conductometric biosensor (24.9
μM).</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>20813591</pmid><doi>10.1016/j.bioelechem.2010.07.006</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-1354-9273</orcidid></addata></record> |
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language | eng |
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source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
subjects | Analytical chemistry Animals Biochemistry Biochemistry, Molecular Biology Biosensing Techniques - methods Catalase Catalase - chemistry Catalase - metabolism Cattle Chemical Sciences Conductometric biosensor Conductometry - methods Cyanide Cyanides - chemistry Cyanides - metabolism Dielectric Spectroscopy - methods Electrochemistry - methods Electrodes Enzyme Inhibitors - chemistry Enzyme Inhibitors - metabolism Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Hydrogen Peroxide - metabolism I 50 Impedimetric biosensor Inhibition Life Sciences Ligands Limit of Detection |
title | Impedance spectroscopy and conductometric biosensing for probing catalase reaction with cyanide as ligand and inhibitor |
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