High levels of multidrug resistance in clinical isolates of Gram-negative pathogens from Nigeria
Abstract In Nigeria, quinolones and β-lactam antibiotics are widely used to treat bacterial infections. This study aimed to identify the prevalence of resistance to these drugs and to determine the mechanisms of resistance to these agents. In total, 134 non-duplicate, Gram-negative enteric isolates...
Gespeichert in:
| Veröffentlicht in: | International journal of antimicrobial agents 2011-01, Vol.37 (1), p.62-66 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 66 |
|---|---|
| container_issue | 1 |
| container_start_page | 62 |
| container_title | International journal of antimicrobial agents |
| container_volume | 37 |
| creator | Ogbolu, D.O Daini, O.A Ogunledun, A Alli, A.O Webber, M.A |
| description | Abstract In Nigeria, quinolones and β-lactam antibiotics are widely used to treat bacterial infections. This study aimed to identify the prevalence of resistance to these drugs and to determine the mechanisms of resistance to these agents. In total, 134 non-duplicate, Gram-negative enteric isolates of 13 species from different hospitals were investigated for susceptibility to a panel of antibiotics, carriage of plasmid-mediated quinolone and β-lactam resistance genes, production of extended-spectrum β-lactamases (ESBLs), and mutations within topoisomerase genes. The level of resistance to all antibiotics tested was extremely high, with minimum inhibitory concentrations for 90% of the organisms (MIC90 values) of ≥256 μg/mL for all drugs. Of the 134 isolates, 92 had mutations within the quinolone resistance-determining region (QRDR) of gyrA or within gyrA and parC . In addition, the plasmid-mediated quinolone resistance genes qnrA , qnrB , aac(6 ′ )-Ib-cr and qepA were identified. The qnrD allele, which has previously only been found in Salmonella isolates from China, was identified in two Proteus isolates and one Pseudomonas isolate. Of the 134 isolates, 23 (17.2%) carried aac(6 ′ )-Ib-cr , 11 (8.2%) carried a qnr variant and 5 (3.7%) were positive for qepA . Twenty-eight isolates (20.9%) produced ESBL variants, with a CTX-M variant being carried by 25 isolates (18.7%). In addition, six isolates (4.5%) carried ampC variants [ACT-1 (1 isolate), DHA-1 (4 isolates) and CMY-2 (1 isolate)]. This study demonstrates a very high level of multidrug resistance amongst Gram-negative enteric bacilli isolated from different sites from patients in Nigerian hospitals as well as the presence of a variety of plasmid-associated resistance genes, including some identified from Africa for the first time. |
| doi_str_mv | 10.1016/j.ijantimicag.2010.08.019 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_00651642v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0924857910004127</els_id><sourcerecordid>818645381</sourcerecordid><originalsourceid>FETCH-LOGICAL-c570t-1423e1c0f625f1e797ff1c41ea5810a806cd1e18bc2099a7763eb9179d04b0663</originalsourceid><addsrcrecordid>eNqNksGO0zAQhiMEYsvCK0A4IMQhZcZJbOeCtKpgi1TBYdmzcZ1J6pLExU4q7dvj0O6COHGyNPrmH2u-SZLXCEsE5O_3S7vXw2h7a3S7ZBDrIJeA1aNkgVKwTFSYP04WULEik6WoLpJnIewBsMyL8mlywRBEkQu-SL6vbbtLOzpSF1LXpP3Ujbb2U5t6CjaMejCU2iE1nR3itC61wXV6pN_wtdd9NlCrR3uk9KDHnWtpCGnjXZ9-sS15q58nTxrdBXpxfi-T208fv63W2ebr9efV1SYzpYAxw4LlhAYazsoGSVSiadAUSLqUCFoCNzUSyq1hUFVaCJ7TtkJR1VBsgfP8Mnl3yt3pTh287bW_U05btb7aqLkGwEvkBTtiZN-e2IN3PycKo-ptMNR1eiA3BSVR8qLM5UxWJ9J4F4Kn5iEaQc0u1F795ULNLhRIFV3E3pfnKdO2p_qh8375EXhzBnSIq218XLYNf7hcQMH4HPTqxDXaKd36yNzexEllFCpZjnPS6kREi3S05FUwlqK62noyo6qd_a8Pf_gn5d76D7qjsHeTH6JChSowBepmPq_5uhAACmQi_wUVqcqh</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>818645381</pqid></control><display><type>article</type><title>High levels of multidrug resistance in clinical isolates of Gram-negative pathogens from Nigeria</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Ogbolu, D.O ; Daini, O.A ; Ogunledun, A ; Alli, A.O ; Webber, M.A</creator><creatorcontrib>Ogbolu, D.O ; Daini, O.A ; Ogunledun, A ; Alli, A.O ; Webber, M.A</creatorcontrib><description>Abstract In Nigeria, quinolones and β-lactam antibiotics are widely used to treat bacterial infections. This study aimed to identify the prevalence of resistance to these drugs and to determine the mechanisms of resistance to these agents. In total, 134 non-duplicate, Gram-negative enteric isolates of 13 species from different hospitals were investigated for susceptibility to a panel of antibiotics, carriage of plasmid-mediated quinolone and β-lactam resistance genes, production of extended-spectrum β-lactamases (ESBLs), and mutations within topoisomerase genes. The level of resistance to all antibiotics tested was extremely high, with minimum inhibitory concentrations for 90% of the organisms (MIC90 values) of ≥256 μg/mL for all drugs. Of the 134 isolates, 92 had mutations within the quinolone resistance-determining region (QRDR) of gyrA or within gyrA and parC . In addition, the plasmid-mediated quinolone resistance genes qnrA , qnrB , aac(6 ′ )-Ib-cr and qepA were identified. The qnrD allele, which has previously only been found in Salmonella isolates from China, was identified in two Proteus isolates and one Pseudomonas isolate. Of the 134 isolates, 23 (17.2%) carried aac(6 ′ )-Ib-cr , 11 (8.2%) carried a qnr variant and 5 (3.7%) were positive for qepA . Twenty-eight isolates (20.9%) produced ESBL variants, with a CTX-M variant being carried by 25 isolates (18.7%). In addition, six isolates (4.5%) carried ampC variants [ACT-1 (1 isolate), DHA-1 (4 isolates) and CMY-2 (1 isolate)]. This study demonstrates a very high level of multidrug resistance amongst Gram-negative enteric bacilli isolated from different sites from patients in Nigerian hospitals as well as the presence of a variety of plasmid-associated resistance genes, including some identified from Africa for the first time.</description><identifier>ISSN: 0924-8579</identifier><identifier>EISSN: 1872-7913</identifier><identifier>DOI: 10.1016/j.ijantimicag.2010.08.019</identifier><identifier>PMID: 21074376</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>alleles ; Anti-Bacterial Agents - pharmacology ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; bacterial infections ; beta-lactam antibiotics ; beta-lactamase ; beta-Lactamases - genetics ; Biological and medical sciences ; DNA, Bacterial - genetics ; Drug Resistance, Multiple, Bacterial ; drugs ; Enterobacteriaceae - drug effects ; Enterobacteriaceae - isolation & purification ; ESBL ; Extended-spectrum β-lactamase ; Gram-Negative Bacterial Infections - microbiology ; hospitals ; Humans ; Infectious Disease ; Medical sciences ; Microbial Sensitivity Tests ; minimum inhibitory concentration ; multiple drug resistance ; mutation ; Nigeria ; pathogens ; patients ; Pharmacology. Drug treatments ; Plasmids - analysis ; Proteus ; Pseudomonas ; Pseudomonas - drug effects ; Pseudomonas - isolation & purification ; Quinolone ; quinolones ; resistance mechanisms ; Salmonella</subject><ispartof>International journal of antimicrobial agents, 2011-01, Vol.37 (1), p.62-66</ispartof><rights>Elsevier B.V. and the International Society of Chemotherapy</rights><rights>2010 Elsevier B.V. and the International Society of Chemotherapy</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c570t-1423e1c0f625f1e797ff1c41ea5810a806cd1e18bc2099a7763eb9179d04b0663</citedby><cites>FETCH-LOGICAL-c570t-1423e1c0f625f1e797ff1c41ea5810a806cd1e18bc2099a7763eb9179d04b0663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijantimicag.2010.08.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23704269$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21074376$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00651642$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Ogbolu, D.O</creatorcontrib><creatorcontrib>Daini, O.A</creatorcontrib><creatorcontrib>Ogunledun, A</creatorcontrib><creatorcontrib>Alli, A.O</creatorcontrib><creatorcontrib>Webber, M.A</creatorcontrib><title>High levels of multidrug resistance in clinical isolates of Gram-negative pathogens from Nigeria</title><title>International journal of antimicrobial agents</title><addtitle>Int J Antimicrob Agents</addtitle><description>Abstract In Nigeria, quinolones and β-lactam antibiotics are widely used to treat bacterial infections. This study aimed to identify the prevalence of resistance to these drugs and to determine the mechanisms of resistance to these agents. In total, 134 non-duplicate, Gram-negative enteric isolates of 13 species from different hospitals were investigated for susceptibility to a panel of antibiotics, carriage of plasmid-mediated quinolone and β-lactam resistance genes, production of extended-spectrum β-lactamases (ESBLs), and mutations within topoisomerase genes. The level of resistance to all antibiotics tested was extremely high, with minimum inhibitory concentrations for 90% of the organisms (MIC90 values) of ≥256 μg/mL for all drugs. Of the 134 isolates, 92 had mutations within the quinolone resistance-determining region (QRDR) of gyrA or within gyrA and parC . In addition, the plasmid-mediated quinolone resistance genes qnrA , qnrB , aac(6 ′ )-Ib-cr and qepA were identified. The qnrD allele, which has previously only been found in Salmonella isolates from China, was identified in two Proteus isolates and one Pseudomonas isolate. Of the 134 isolates, 23 (17.2%) carried aac(6 ′ )-Ib-cr , 11 (8.2%) carried a qnr variant and 5 (3.7%) were positive for qepA . Twenty-eight isolates (20.9%) produced ESBL variants, with a CTX-M variant being carried by 25 isolates (18.7%). In addition, six isolates (4.5%) carried ampC variants [ACT-1 (1 isolate), DHA-1 (4 isolates) and CMY-2 (1 isolate)]. This study demonstrates a very high level of multidrug resistance amongst Gram-negative enteric bacilli isolated from different sites from patients in Nigerian hospitals as well as the presence of a variety of plasmid-associated resistance genes, including some identified from Africa for the first time.</description><subject>alleles</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>bacterial infections</subject><subject>beta-lactam antibiotics</subject><subject>beta-lactamase</subject><subject>beta-Lactamases - genetics</subject><subject>Biological and medical sciences</subject><subject>DNA, Bacterial - genetics</subject><subject>Drug Resistance, Multiple, Bacterial</subject><subject>drugs</subject><subject>Enterobacteriaceae - drug effects</subject><subject>Enterobacteriaceae - isolation & purification</subject><subject>ESBL</subject><subject>Extended-spectrum β-lactamase</subject><subject>Gram-Negative Bacterial Infections - microbiology</subject><subject>hospitals</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Medical sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>minimum inhibitory concentration</subject><subject>multiple drug resistance</subject><subject>mutation</subject><subject>Nigeria</subject><subject>pathogens</subject><subject>patients</subject><subject>Pharmacology. Drug treatments</subject><subject>Plasmids - analysis</subject><subject>Proteus</subject><subject>Pseudomonas</subject><subject>Pseudomonas - drug effects</subject><subject>Pseudomonas - isolation & purification</subject><subject>Quinolone</subject><subject>quinolones</subject><subject>resistance mechanisms</subject><subject>Salmonella</subject><issn>0924-8579</issn><issn>1872-7913</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNksGO0zAQhiMEYsvCK0A4IMQhZcZJbOeCtKpgi1TBYdmzcZ1J6pLExU4q7dvj0O6COHGyNPrmH2u-SZLXCEsE5O_3S7vXw2h7a3S7ZBDrIJeA1aNkgVKwTFSYP04WULEik6WoLpJnIewBsMyL8mlywRBEkQu-SL6vbbtLOzpSF1LXpP3Ujbb2U5t6CjaMejCU2iE1nR3itC61wXV6pN_wtdd9NlCrR3uk9KDHnWtpCGnjXZ9-sS15q58nTxrdBXpxfi-T208fv63W2ebr9efV1SYzpYAxw4LlhAYazsoGSVSiadAUSLqUCFoCNzUSyq1hUFVaCJ7TtkJR1VBsgfP8Mnl3yt3pTh287bW_U05btb7aqLkGwEvkBTtiZN-e2IN3PycKo-ptMNR1eiA3BSVR8qLM5UxWJ9J4F4Kn5iEaQc0u1F795ULNLhRIFV3E3pfnKdO2p_qh8375EXhzBnSIq218XLYNf7hcQMH4HPTqxDXaKd36yNzexEllFCpZjnPS6kREi3S05FUwlqK62noyo6qd_a8Pf_gn5d76D7qjsHeTH6JChSowBepmPq_5uhAACmQi_wUVqcqh</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Ogbolu, D.O</creator><creator>Daini, O.A</creator><creator>Ogunledun, A</creator><creator>Alli, A.O</creator><creator>Webber, M.A</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope></search><sort><creationdate>20110101</creationdate><title>High levels of multidrug resistance in clinical isolates of Gram-negative pathogens from Nigeria</title><author>Ogbolu, D.O ; Daini, O.A ; Ogunledun, A ; Alli, A.O ; Webber, M.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c570t-1423e1c0f625f1e797ff1c41ea5810a806cd1e18bc2099a7763eb9179d04b0663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>alleles</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>bacterial infections</topic><topic>beta-lactam antibiotics</topic><topic>beta-lactamase</topic><topic>beta-Lactamases - genetics</topic><topic>Biological and medical sciences</topic><topic>DNA, Bacterial - genetics</topic><topic>Drug Resistance, Multiple, Bacterial</topic><topic>drugs</topic><topic>Enterobacteriaceae - drug effects</topic><topic>Enterobacteriaceae - isolation & purification</topic><topic>ESBL</topic><topic>Extended-spectrum β-lactamase</topic><topic>Gram-Negative Bacterial Infections - microbiology</topic><topic>hospitals</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Medical sciences</topic><topic>Microbial Sensitivity Tests</topic><topic>minimum inhibitory concentration</topic><topic>multiple drug resistance</topic><topic>mutation</topic><topic>Nigeria</topic><topic>pathogens</topic><topic>patients</topic><topic>Pharmacology. Drug treatments</topic><topic>Plasmids - analysis</topic><topic>Proteus</topic><topic>Pseudomonas</topic><topic>Pseudomonas - drug effects</topic><topic>Pseudomonas - isolation & purification</topic><topic>Quinolone</topic><topic>quinolones</topic><topic>resistance mechanisms</topic><topic>Salmonella</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ogbolu, D.O</creatorcontrib><creatorcontrib>Daini, O.A</creatorcontrib><creatorcontrib>Ogunledun, A</creatorcontrib><creatorcontrib>Alli, A.O</creatorcontrib><creatorcontrib>Webber, M.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>International journal of antimicrobial agents</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ogbolu, D.O</au><au>Daini, O.A</au><au>Ogunledun, A</au><au>Alli, A.O</au><au>Webber, M.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High levels of multidrug resistance in clinical isolates of Gram-negative pathogens from Nigeria</atitle><jtitle>International journal of antimicrobial agents</jtitle><addtitle>Int J Antimicrob Agents</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>37</volume><issue>1</issue><spage>62</spage><epage>66</epage><pages>62-66</pages><issn>0924-8579</issn><eissn>1872-7913</eissn><abstract>Abstract In Nigeria, quinolones and β-lactam antibiotics are widely used to treat bacterial infections. This study aimed to identify the prevalence of resistance to these drugs and to determine the mechanisms of resistance to these agents. In total, 134 non-duplicate, Gram-negative enteric isolates of 13 species from different hospitals were investigated for susceptibility to a panel of antibiotics, carriage of plasmid-mediated quinolone and β-lactam resistance genes, production of extended-spectrum β-lactamases (ESBLs), and mutations within topoisomerase genes. The level of resistance to all antibiotics tested was extremely high, with minimum inhibitory concentrations for 90% of the organisms (MIC90 values) of ≥256 μg/mL for all drugs. Of the 134 isolates, 92 had mutations within the quinolone resistance-determining region (QRDR) of gyrA or within gyrA and parC . In addition, the plasmid-mediated quinolone resistance genes qnrA , qnrB , aac(6 ′ )-Ib-cr and qepA were identified. The qnrD allele, which has previously only been found in Salmonella isolates from China, was identified in two Proteus isolates and one Pseudomonas isolate. Of the 134 isolates, 23 (17.2%) carried aac(6 ′ )-Ib-cr , 11 (8.2%) carried a qnr variant and 5 (3.7%) were positive for qepA . Twenty-eight isolates (20.9%) produced ESBL variants, with a CTX-M variant being carried by 25 isolates (18.7%). In addition, six isolates (4.5%) carried ampC variants [ACT-1 (1 isolate), DHA-1 (4 isolates) and CMY-2 (1 isolate)]. This study demonstrates a very high level of multidrug resistance amongst Gram-negative enteric bacilli isolated from different sites from patients in Nigerian hospitals as well as the presence of a variety of plasmid-associated resistance genes, including some identified from Africa for the first time.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>21074376</pmid><doi>10.1016/j.ijantimicag.2010.08.019</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0924-8579 |
| ispartof | International journal of antimicrobial agents, 2011-01, Vol.37 (1), p.62-66 |
| issn | 0924-8579 1872-7913 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_00651642v1 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | alleles Anti-Bacterial Agents - pharmacology Antibiotics. Antiinfectious agents. Antiparasitic agents bacterial infections beta-lactam antibiotics beta-lactamase beta-Lactamases - genetics Biological and medical sciences DNA, Bacterial - genetics Drug Resistance, Multiple, Bacterial drugs Enterobacteriaceae - drug effects Enterobacteriaceae - isolation & purification ESBL Extended-spectrum β-lactamase Gram-Negative Bacterial Infections - microbiology hospitals Humans Infectious Disease Medical sciences Microbial Sensitivity Tests minimum inhibitory concentration multiple drug resistance mutation Nigeria pathogens patients Pharmacology. Drug treatments Plasmids - analysis Proteus Pseudomonas Pseudomonas - drug effects Pseudomonas - isolation & purification Quinolone quinolones resistance mechanisms Salmonella |
| title | High levels of multidrug resistance in clinical isolates of Gram-negative pathogens from Nigeria |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T17%3A19%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=High%20levels%20of%20multidrug%20resistance%20in%20clinical%20isolates%20of%20Gram-negative%20pathogens%20from%20Nigeria&rft.jtitle=International%20journal%20of%20antimicrobial%20agents&rft.au=Ogbolu,%20D.O&rft.date=2011-01-01&rft.volume=37&rft.issue=1&rft.spage=62&rft.epage=66&rft.pages=62-66&rft.issn=0924-8579&rft.eissn=1872-7913&rft_id=info:doi/10.1016/j.ijantimicag.2010.08.019&rft_dat=%3Cproquest_hal_p%3E818645381%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=818645381&rft_id=info:pmid/21074376&rft_els_id=S0924857910004127&rfr_iscdi=true |