Inhibition of SRY-Calmodulin Complex Formation Induces Ectopic Expression of Ovarian Cell Markers in Developing XY Gonads
The transcription factor sex-determining region of the Y chromosome (SRY) plays a key role in human sex determination, because mutations in SRY cause disorders of sex development in XY individuals. During gonadal development, Sry in pre-Sertoli cells activates Sox9 gene transcription, committing the...
Gespeichert in:
| Veröffentlicht in: | Endocrinology (Philadelphia) 2011-07, Vol.152 (7), p.2883-2893 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2893 |
|---|---|
| container_issue | 7 |
| container_start_page | 2883 |
| container_title | Endocrinology (Philadelphia) |
| container_volume | 152 |
| creator | Sim, Helena Argentaro, Anthony Czech, Daniel P Bagheri-Fam, Stefan Sinclair, Andrew H Koopman, Peter Boizet-Bonhoure, Brigitte Poulat, Francis Harley, Vincent R |
| description | The transcription factor sex-determining region of the Y chromosome (SRY) plays a key role in human sex determination, because mutations in SRY cause disorders of sex development in XY individuals. During gonadal development, Sry in pre-Sertoli cells activates Sox9 gene transcription, committing the fate of the bipotential gonad to become a testis rather than an ovary. The high-mobility group domain of human SRY contains two independent nuclear localization signals, one bound by calmodulin (CaM) and the other by importin-β. Although XY females carry SRY mutations in these nuclear localization signals that affect SRY nuclear import in transfected cells, it is not known whether these transport mechanisms are essential for gonadal development and sex determination. Here, we show that mouse Sry protein binds CaM and that a CaM antagonist reduces CaM binding, nuclear accumulation, and transcriptional activity of Sry in transfected cells. CaM antagonist treatment of cultured, sexually indifferent XY mouse fetal gonads led to reduced expression of the Sry target gene Sox9, defects in testicular cord formation, and ectopic expression of the ovarian markers Rspondin1 and forkhead box L2. These results indicate the importance of CaM for SRY nuclear import, transcriptional activity, testis differentiation, and sex determination. |
| doi_str_mv | 10.1210/en.2010-1475 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_00616458v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1210/en.2010-1475</oup_id><sourcerecordid>873707508</sourcerecordid><originalsourceid>FETCH-LOGICAL-c595t-e9a1872eafc806615e90a68c5b1ce626d3c4b33ca2bf4a24cd478ba00aac4aa3</originalsourceid><addsrcrecordid>eNp90VFv0zAQB_AIgVgZvPGMIiEESGT4YjtOHqfSbZWKJsEe2JN1cRzmkdiZ3VTbt8dtw4YQ8GTZ-t35Tv8keQnkCHIgH7U9ygmQDJjgj5IZVIxnAgR5nMwIAZqJPBcHybMQruOVMUafJgc5cF5SYLPkbmmvTG3WxtnUtenXL5fZHLveNWNnbDp3_dDp2_TE-R53ZmmbUemQLtTaDUali9vB6xCm8vMNeoOxTndd-hn9D-1DGvt80hvdRW-_p98u01NnsQnPkyctdkG_mM7D5OJkcTE_y1bnp8v58SpTvOLrTFcIpcg1tqokRQFcVwSLUvEalC7yoqGK1ZQqzOuWYc5Uw0RZIyGIiiHSw-T9vu0VdnLwpkd_Jx0aeXa8kts3QgooGC83EO3bvR28uxl1WMveBBV3QavdGGQpqCCCkzLKd_-VQEBUnDFOIn39B712o7dxZUmBkoKUsGv4Ya-UdyF43d7PCkRuc5baym3Ocptz5K-mpmPd6-Ye_wo2gjcTwKCwaz1aZcKDY7ngJfy2shuHf32ZTV_SvdS2ccobq3fJP2zz10F_AlRAy6w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3130608108</pqid></control><display><type>article</type><title>Inhibition of SRY-Calmodulin Complex Formation Induces Ectopic Expression of Ovarian Cell Markers in Developing XY Gonads</title><source>MEDLINE</source><source>Journals@Ovid Complete</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Sim, Helena ; Argentaro, Anthony ; Czech, Daniel P ; Bagheri-Fam, Stefan ; Sinclair, Andrew H ; Koopman, Peter ; Boizet-Bonhoure, Brigitte ; Poulat, Francis ; Harley, Vincent R</creator><creatorcontrib>Sim, Helena ; Argentaro, Anthony ; Czech, Daniel P ; Bagheri-Fam, Stefan ; Sinclair, Andrew H ; Koopman, Peter ; Boizet-Bonhoure, Brigitte ; Poulat, Francis ; Harley, Vincent R</creatorcontrib><description>The transcription factor sex-determining region of the Y chromosome (SRY) plays a key role in human sex determination, because mutations in SRY cause disorders of sex development in XY individuals. During gonadal development, Sry in pre-Sertoli cells activates Sox9 gene transcription, committing the fate of the bipotential gonad to become a testis rather than an ovary. The high-mobility group domain of human SRY contains two independent nuclear localization signals, one bound by calmodulin (CaM) and the other by importin-β. Although XY females carry SRY mutations in these nuclear localization signals that affect SRY nuclear import in transfected cells, it is not known whether these transport mechanisms are essential for gonadal development and sex determination. Here, we show that mouse Sry protein binds CaM and that a CaM antagonist reduces CaM binding, nuclear accumulation, and transcriptional activity of Sry in transfected cells. CaM antagonist treatment of cultured, sexually indifferent XY mouse fetal gonads led to reduced expression of the Sry target gene Sox9, defects in testicular cord formation, and ectopic expression of the ovarian markers Rspondin1 and forkhead box L2. These results indicate the importance of CaM for SRY nuclear import, transcriptional activity, testis differentiation, and sex determination.</description><identifier>ISSN: 0013-7227</identifier><identifier>EISSN: 1945-7170</identifier><identifier>DOI: 10.1210/en.2010-1475</identifier><identifier>PMID: 21558314</identifier><identifier>CODEN: ENDOAO</identifier><language>eng</language><publisher>Chevy Chase, MD: Endocrine Society</publisher><subject>Active Transport, Cell Nucleus - drug effects ; Animals ; Antigens, Differentiation - metabolism ; Biological and medical sciences ; Calcium-binding protein ; Calmodulin ; Calmodulin - antagonists & inhibitors ; Calmodulin - metabolism ; Calmodulin-Binding Proteins - genetics ; Calmodulin-Binding Proteins - metabolism ; Cell Nucleus - metabolism ; Cell Nucleus - ultrastructure ; Cercopithecus aethiops ; Complex formation ; COS Cells ; Differentiation ; Ectopic expression ; Embryo, Mammalian - cytology ; Embryo, Mammalian - metabolism ; Fetuses ; Forkhead Box Protein L2 ; Forkhead protein ; Forkhead Transcription Factors - metabolism ; Fundamental and applied biological sciences. Psychology ; Genetics ; Gonads ; Imports ; Life Sciences ; Localization ; Male ; Mice ; Mutation ; Nuclear transport ; Organ Culture Techniques ; Ovaries ; Protein transport ; Recombinant Proteins - metabolism ; Sertoli cells ; Sex ; Sex determination ; Sex Determination Processes - drug effects ; Sex-Determining Region Y Protein - genetics ; Sex-Determining Region Y Protein - metabolism ; Sox9 protein ; SOX9 Transcription Factor - genetics ; SOX9 Transcription Factor - metabolism ; Spermatic Cord - drug effects ; Spermatogenesis ; Testes ; Testis - physiology ; Testis - ultrastructure ; Thrombospondins - metabolism ; Transcription factors ; Transcriptional Activation - drug effects ; Vertebrates: endocrinology ; Y chromosome ; Y chromosomes</subject><ispartof>Endocrinology (Philadelphia), 2011-07, Vol.152 (7), p.2883-2893</ispartof><rights>Copyright © 2011 by The Endocrine Society</rights><rights>Copyright © 2011 by The Endocrine Society 2011</rights><rights>2015 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c595t-e9a1872eafc806615e90a68c5b1ce626d3c4b33ca2bf4a24cd478ba00aac4aa3</citedby><cites>FETCH-LOGICAL-c595t-e9a1872eafc806615e90a68c5b1ce626d3c4b33ca2bf4a24cd478ba00aac4aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24275811$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21558314$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00616458$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Sim, Helena</creatorcontrib><creatorcontrib>Argentaro, Anthony</creatorcontrib><creatorcontrib>Czech, Daniel P</creatorcontrib><creatorcontrib>Bagheri-Fam, Stefan</creatorcontrib><creatorcontrib>Sinclair, Andrew H</creatorcontrib><creatorcontrib>Koopman, Peter</creatorcontrib><creatorcontrib>Boizet-Bonhoure, Brigitte</creatorcontrib><creatorcontrib>Poulat, Francis</creatorcontrib><creatorcontrib>Harley, Vincent R</creatorcontrib><title>Inhibition of SRY-Calmodulin Complex Formation Induces Ectopic Expression of Ovarian Cell Markers in Developing XY Gonads</title><title>Endocrinology (Philadelphia)</title><addtitle>Endocrinology</addtitle><description>The transcription factor sex-determining region of the Y chromosome (SRY) plays a key role in human sex determination, because mutations in SRY cause disorders of sex development in XY individuals. During gonadal development, Sry in pre-Sertoli cells activates Sox9 gene transcription, committing the fate of the bipotential gonad to become a testis rather than an ovary. The high-mobility group domain of human SRY contains two independent nuclear localization signals, one bound by calmodulin (CaM) and the other by importin-β. Although XY females carry SRY mutations in these nuclear localization signals that affect SRY nuclear import in transfected cells, it is not known whether these transport mechanisms are essential for gonadal development and sex determination. Here, we show that mouse Sry protein binds CaM and that a CaM antagonist reduces CaM binding, nuclear accumulation, and transcriptional activity of Sry in transfected cells. CaM antagonist treatment of cultured, sexually indifferent XY mouse fetal gonads led to reduced expression of the Sry target gene Sox9, defects in testicular cord formation, and ectopic expression of the ovarian markers Rspondin1 and forkhead box L2. These results indicate the importance of CaM for SRY nuclear import, transcriptional activity, testis differentiation, and sex determination.</description><subject>Active Transport, Cell Nucleus - drug effects</subject><subject>Animals</subject><subject>Antigens, Differentiation - metabolism</subject><subject>Biological and medical sciences</subject><subject>Calcium-binding protein</subject><subject>Calmodulin</subject><subject>Calmodulin - antagonists & inhibitors</subject><subject>Calmodulin - metabolism</subject><subject>Calmodulin-Binding Proteins - genetics</subject><subject>Calmodulin-Binding Proteins - metabolism</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Cercopithecus aethiops</subject><subject>Complex formation</subject><subject>COS Cells</subject><subject>Differentiation</subject><subject>Ectopic expression</subject><subject>Embryo, Mammalian - cytology</subject><subject>Embryo, Mammalian - metabolism</subject><subject>Fetuses</subject><subject>Forkhead Box Protein L2</subject><subject>Forkhead protein</subject><subject>Forkhead Transcription Factors - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Gonads</subject><subject>Imports</subject><subject>Life Sciences</subject><subject>Localization</subject><subject>Male</subject><subject>Mice</subject><subject>Mutation</subject><subject>Nuclear transport</subject><subject>Organ Culture Techniques</subject><subject>Ovaries</subject><subject>Protein transport</subject><subject>Recombinant Proteins - metabolism</subject><subject>Sertoli cells</subject><subject>Sex</subject><subject>Sex determination</subject><subject>Sex Determination Processes - drug effects</subject><subject>Sex-Determining Region Y Protein - genetics</subject><subject>Sex-Determining Region Y Protein - metabolism</subject><subject>Sox9 protein</subject><subject>SOX9 Transcription Factor - genetics</subject><subject>SOX9 Transcription Factor - metabolism</subject><subject>Spermatic Cord - drug effects</subject><subject>Spermatogenesis</subject><subject>Testes</subject><subject>Testis - physiology</subject><subject>Testis - ultrastructure</subject><subject>Thrombospondins - metabolism</subject><subject>Transcription factors</subject><subject>Transcriptional Activation - drug effects</subject><subject>Vertebrates: endocrinology</subject><subject>Y chromosome</subject><subject>Y chromosomes</subject><issn>0013-7227</issn><issn>1945-7170</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90VFv0zAQB_AIgVgZvPGMIiEESGT4YjtOHqfSbZWKJsEe2JN1cRzmkdiZ3VTbt8dtw4YQ8GTZ-t35Tv8keQnkCHIgH7U9ygmQDJjgj5IZVIxnAgR5nMwIAZqJPBcHybMQruOVMUafJgc5cF5SYLPkbmmvTG3WxtnUtenXL5fZHLveNWNnbDp3_dDp2_TE-R53ZmmbUemQLtTaDUali9vB6xCm8vMNeoOxTndd-hn9D-1DGvt80hvdRW-_p98u01NnsQnPkyctdkG_mM7D5OJkcTE_y1bnp8v58SpTvOLrTFcIpcg1tqokRQFcVwSLUvEalC7yoqGK1ZQqzOuWYc5Uw0RZIyGIiiHSw-T9vu0VdnLwpkd_Jx0aeXa8kts3QgooGC83EO3bvR28uxl1WMveBBV3QavdGGQpqCCCkzLKd_-VQEBUnDFOIn39B712o7dxZUmBkoKUsGv4Ya-UdyF43d7PCkRuc5baym3Ocptz5K-mpmPd6-Ye_wo2gjcTwKCwaz1aZcKDY7ngJfy2shuHf32ZTV_SvdS2ccobq3fJP2zz10F_AlRAy6w</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>Sim, Helena</creator><creator>Argentaro, Anthony</creator><creator>Czech, Daniel P</creator><creator>Bagheri-Fam, Stefan</creator><creator>Sinclair, Andrew H</creator><creator>Koopman, Peter</creator><creator>Boizet-Bonhoure, Brigitte</creator><creator>Poulat, Francis</creator><creator>Harley, Vincent R</creator><general>Endocrine Society</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20110701</creationdate><title>Inhibition of SRY-Calmodulin Complex Formation Induces Ectopic Expression of Ovarian Cell Markers in Developing XY Gonads</title><author>Sim, Helena ; Argentaro, Anthony ; Czech, Daniel P ; Bagheri-Fam, Stefan ; Sinclair, Andrew H ; Koopman, Peter ; Boizet-Bonhoure, Brigitte ; Poulat, Francis ; Harley, Vincent R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c595t-e9a1872eafc806615e90a68c5b1ce626d3c4b33ca2bf4a24cd478ba00aac4aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Active Transport, Cell Nucleus - drug effects</topic><topic>Animals</topic><topic>Antigens, Differentiation - metabolism</topic><topic>Biological and medical sciences</topic><topic>Calcium-binding protein</topic><topic>Calmodulin</topic><topic>Calmodulin - antagonists & inhibitors</topic><topic>Calmodulin - metabolism</topic><topic>Calmodulin-Binding Proteins - genetics</topic><topic>Calmodulin-Binding Proteins - metabolism</topic><topic>Cell Nucleus - metabolism</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Cercopithecus aethiops</topic><topic>Complex formation</topic><topic>COS Cells</topic><topic>Differentiation</topic><topic>Ectopic expression</topic><topic>Embryo, Mammalian - cytology</topic><topic>Embryo, Mammalian - metabolism</topic><topic>Fetuses</topic><topic>Forkhead Box Protein L2</topic><topic>Forkhead protein</topic><topic>Forkhead Transcription Factors - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>Gonads</topic><topic>Imports</topic><topic>Life Sciences</topic><topic>Localization</topic><topic>Male</topic><topic>Mice</topic><topic>Mutation</topic><topic>Nuclear transport</topic><topic>Organ Culture Techniques</topic><topic>Ovaries</topic><topic>Protein transport</topic><topic>Recombinant Proteins - metabolism</topic><topic>Sertoli cells</topic><topic>Sex</topic><topic>Sex determination</topic><topic>Sex Determination Processes - drug effects</topic><topic>Sex-Determining Region Y Protein - genetics</topic><topic>Sex-Determining Region Y Protein - metabolism</topic><topic>Sox9 protein</topic><topic>SOX9 Transcription Factor - genetics</topic><topic>SOX9 Transcription Factor - metabolism</topic><topic>Spermatic Cord - drug effects</topic><topic>Spermatogenesis</topic><topic>Testes</topic><topic>Testis - physiology</topic><topic>Testis - ultrastructure</topic><topic>Thrombospondins - metabolism</topic><topic>Transcription factors</topic><topic>Transcriptional Activation - drug effects</topic><topic>Vertebrates: endocrinology</topic><topic>Y chromosome</topic><topic>Y chromosomes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sim, Helena</creatorcontrib><creatorcontrib>Argentaro, Anthony</creatorcontrib><creatorcontrib>Czech, Daniel P</creatorcontrib><creatorcontrib>Bagheri-Fam, Stefan</creatorcontrib><creatorcontrib>Sinclair, Andrew H</creatorcontrib><creatorcontrib>Koopman, Peter</creatorcontrib><creatorcontrib>Boizet-Bonhoure, Brigitte</creatorcontrib><creatorcontrib>Poulat, Francis</creatorcontrib><creatorcontrib>Harley, Vincent R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Endocrinology (Philadelphia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sim, Helena</au><au>Argentaro, Anthony</au><au>Czech, Daniel P</au><au>Bagheri-Fam, Stefan</au><au>Sinclair, Andrew H</au><au>Koopman, Peter</au><au>Boizet-Bonhoure, Brigitte</au><au>Poulat, Francis</au><au>Harley, Vincent R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of SRY-Calmodulin Complex Formation Induces Ectopic Expression of Ovarian Cell Markers in Developing XY Gonads</atitle><jtitle>Endocrinology (Philadelphia)</jtitle><addtitle>Endocrinology</addtitle><date>2011-07-01</date><risdate>2011</risdate><volume>152</volume><issue>7</issue><spage>2883</spage><epage>2893</epage><pages>2883-2893</pages><issn>0013-7227</issn><eissn>1945-7170</eissn><coden>ENDOAO</coden><abstract>The transcription factor sex-determining region of the Y chromosome (SRY) plays a key role in human sex determination, because mutations in SRY cause disorders of sex development in XY individuals. During gonadal development, Sry in pre-Sertoli cells activates Sox9 gene transcription, committing the fate of the bipotential gonad to become a testis rather than an ovary. The high-mobility group domain of human SRY contains two independent nuclear localization signals, one bound by calmodulin (CaM) and the other by importin-β. Although XY females carry SRY mutations in these nuclear localization signals that affect SRY nuclear import in transfected cells, it is not known whether these transport mechanisms are essential for gonadal development and sex determination. Here, we show that mouse Sry protein binds CaM and that a CaM antagonist reduces CaM binding, nuclear accumulation, and transcriptional activity of Sry in transfected cells. CaM antagonist treatment of cultured, sexually indifferent XY mouse fetal gonads led to reduced expression of the Sry target gene Sox9, defects in testicular cord formation, and ectopic expression of the ovarian markers Rspondin1 and forkhead box L2. These results indicate the importance of CaM for SRY nuclear import, transcriptional activity, testis differentiation, and sex determination.</abstract><cop>Chevy Chase, MD</cop><pub>Endocrine Society</pub><pmid>21558314</pmid><doi>10.1210/en.2010-1475</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0013-7227 |
| ispartof | Endocrinology (Philadelphia), 2011-07, Vol.152 (7), p.2883-2893 |
| issn | 0013-7227 1945-7170 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_00616458v1 |
| source | MEDLINE; Journals@Ovid Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Active Transport, Cell Nucleus - drug effects Animals Antigens, Differentiation - metabolism Biological and medical sciences Calcium-binding protein Calmodulin Calmodulin - antagonists & inhibitors Calmodulin - metabolism Calmodulin-Binding Proteins - genetics Calmodulin-Binding Proteins - metabolism Cell Nucleus - metabolism Cell Nucleus - ultrastructure Cercopithecus aethiops Complex formation COS Cells Differentiation Ectopic expression Embryo, Mammalian - cytology Embryo, Mammalian - metabolism Fetuses Forkhead Box Protein L2 Forkhead protein Forkhead Transcription Factors - metabolism Fundamental and applied biological sciences. Psychology Genetics Gonads Imports Life Sciences Localization Male Mice Mutation Nuclear transport Organ Culture Techniques Ovaries Protein transport Recombinant Proteins - metabolism Sertoli cells Sex Sex determination Sex Determination Processes - drug effects Sex-Determining Region Y Protein - genetics Sex-Determining Region Y Protein - metabolism Sox9 protein SOX9 Transcription Factor - genetics SOX9 Transcription Factor - metabolism Spermatic Cord - drug effects Spermatogenesis Testes Testis - physiology Testis - ultrastructure Thrombospondins - metabolism Transcription factors Transcriptional Activation - drug effects Vertebrates: endocrinology Y chromosome Y chromosomes |
| title | Inhibition of SRY-Calmodulin Complex Formation Induces Ectopic Expression of Ovarian Cell Markers in Developing XY Gonads |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T16%3A49%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Inhibition%20of%20SRY-Calmodulin%20Complex%20Formation%20Induces%20Ectopic%20Expression%20of%20Ovarian%20Cell%20Markers%20in%20Developing%20XY%20Gonads&rft.jtitle=Endocrinology%20(Philadelphia)&rft.au=Sim,%20Helena&rft.date=2011-07-01&rft.volume=152&rft.issue=7&rft.spage=2883&rft.epage=2893&rft.pages=2883-2893&rft.issn=0013-7227&rft.eissn=1945-7170&rft.coden=ENDOAO&rft_id=info:doi/10.1210/en.2010-1475&rft_dat=%3Cproquest_hal_p%3E873707508%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3130608108&rft_id=info:pmid/21558314&rft_oup_id=10.1210/en.2010-1475&rfr_iscdi=true |