P75 neurotrophin receptor is sequestered in the Golgi apparatus of the U-87 MG human glioblastoma cell line
The P75 neurotrophin receptor (p75NTR) is a cell surface receptor that can induce apoptosis in many cell types. This receptor plays a major role in the development of the central nervous system and is expressed in some adult brain cells. Its implication in cell apoptosis or survival is probably of m...
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| Veröffentlicht in: | International journal of oncology 2011-02, Vol.38 (2), p.391-399 |
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| container_title | International journal of oncology |
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| creator | GIRAUD, Stéphanie LOUM, Elodie BESSETTE, Barbara MATHONNET, Muriel LALLOUE, Fabrice |
| description | The P75 neurotrophin receptor (p75NTR) is a cell surface receptor that can induce apoptosis in many cell types. This receptor plays a major role in the development of the central nervous system and is expressed in some adult brain cells. Its implication in cell apoptosis or survival is probably of major importance in cellular homeostasis and thus p75NTR could be implicated in tumor resistance to death. In this study, we investigated the intracellular expression of p75NTR in a human glioblastoma cell line. Detection of p75NTR receptor in Golgi apparatus by immunofluorescence microscopy, or after Golgi apparatus extraction, could be correlated with a decrease of cell apoptosis leading cells to become tumorous. This hypothesis is supported by a loss of ligand-induced apoptosis in this cell line. Our observations show that p75NTR can be sequestered in the Golgi complex and could then be, in part, responsible for the cell resistance to apoptosis and for brain tumor formation. |
| doi_str_mv | 10.3892/ijo.2010.862 |
| format | Article |
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This receptor plays a major role in the development of the central nervous system and is expressed in some adult brain cells. Its implication in cell apoptosis or survival is probably of major importance in cellular homeostasis and thus p75NTR could be implicated in tumor resistance to death. In this study, we investigated the intracellular expression of p75NTR in a human glioblastoma cell line. Detection of p75NTR receptor in Golgi apparatus by immunofluorescence microscopy, or after Golgi apparatus extraction, could be correlated with a decrease of cell apoptosis leading cells to become tumorous. This hypothesis is supported by a loss of ligand-induced apoptosis in this cell line. Our observations show that p75NTR can be sequestered in the Golgi complex and could then be, in part, responsible for the cell resistance to apoptosis and for brain tumor formation.</description><identifier>ISSN: 1019-6439</identifier><identifier>EISSN: 1791-2423</identifier><identifier>DOI: 10.3892/ijo.2010.862</identifier><identifier>PMID: 21152857</identifier><language>eng</language><publisher>Athens: Editorial Academy of the International Journal of Oncology</publisher><subject>Apoptosis ; Biological and medical sciences ; Blotting, Western ; Brain Neoplasms ; Brain Neoplasms - metabolism ; Brain Neoplasms - pathology ; Cancer ; Cellular Biology ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Fluorescent Antibody Technique ; Glioblastoma ; Glioblastoma - metabolism ; Glioblastoma - pathology ; Glycoside Hydrolases ; Glycoside Hydrolases - metabolism ; Golgi Apparatus ; Golgi Apparatus - metabolism ; Humans ; Immunoenzyme Techniques ; Life Sciences ; Medical sciences ; Membrane Microdomains ; Membrane Microdomains - metabolism ; Neurobiology ; Neurology ; Neurons and Cognition ; Receptor, Nerve Growth Factor ; Receptor, Nerve Growth Factor - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger ; RNA, Messenger - genetics ; Subcellular Fractions ; Tumor Cells, Cultured ; Tumors ; Tumors of the nervous system. Phacomatoses</subject><ispartof>International journal of oncology, 2011-02, Vol.38 (2), p.391-399</ispartof><rights>2015 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-8cb0f14f09f6b4f45db36c1c5e8ff27b1c80877bdda46dbe8c8b3b97330964de3</citedby><orcidid>0000-0003-0382-4948</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23747203$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21152857$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://unilim.hal.science/hal-00597714$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>GIRAUD, Stéphanie</creatorcontrib><creatorcontrib>LOUM, Elodie</creatorcontrib><creatorcontrib>BESSETTE, Barbara</creatorcontrib><creatorcontrib>MATHONNET, Muriel</creatorcontrib><creatorcontrib>LALLOUE, Fabrice</creatorcontrib><title>P75 neurotrophin receptor is sequestered in the Golgi apparatus of the U-87 MG human glioblastoma cell line</title><title>International journal of oncology</title><addtitle>Int J Oncol</addtitle><description>The P75 neurotrophin receptor (p75NTR) is a cell surface receptor that can induce apoptosis in many cell types. This receptor plays a major role in the development of the central nervous system and is expressed in some adult brain cells. Its implication in cell apoptosis or survival is probably of major importance in cellular homeostasis and thus p75NTR could be implicated in tumor resistance to death. In this study, we investigated the intracellular expression of p75NTR in a human glioblastoma cell line. Detection of p75NTR receptor in Golgi apparatus by immunofluorescence microscopy, or after Golgi apparatus extraction, could be correlated with a decrease of cell apoptosis leading cells to become tumorous. This hypothesis is supported by a loss of ligand-induced apoptosis in this cell line. Our observations show that p75NTR can be sequestered in the Golgi complex and could then be, in part, responsible for the cell resistance to apoptosis and for brain tumor formation.</description><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Brain Neoplasms</subject><subject>Brain Neoplasms - metabolism</subject><subject>Brain Neoplasms - pathology</subject><subject>Cancer</subject><subject>Cellular Biology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Flow Cytometry</subject><subject>Fluorescent Antibody Technique</subject><subject>Glioblastoma</subject><subject>Glioblastoma - metabolism</subject><subject>Glioblastoma - pathology</subject><subject>Glycoside Hydrolases</subject><subject>Glycoside Hydrolases - metabolism</subject><subject>Golgi Apparatus</subject><subject>Golgi Apparatus - metabolism</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Life Sciences</subject><subject>Medical sciences</subject><subject>Membrane Microdomains</subject><subject>Membrane Microdomains - metabolism</subject><subject>Neurobiology</subject><subject>Neurology</subject><subject>Neurons and Cognition</subject><subject>Receptor, Nerve Growth Factor</subject><subject>Receptor, Nerve Growth Factor - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger</subject><subject>RNA, Messenger - genetics</subject><subject>Subcellular Fractions</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><subject>Tumors of the nervous system. 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Phacomatoses</topic><toplevel>online_resources</toplevel><creatorcontrib>GIRAUD, Stéphanie</creatorcontrib><creatorcontrib>LOUM, Elodie</creatorcontrib><creatorcontrib>BESSETTE, Barbara</creatorcontrib><creatorcontrib>MATHONNET, Muriel</creatorcontrib><creatorcontrib>LALLOUE, Fabrice</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>International journal of oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GIRAUD, Stéphanie</au><au>LOUM, Elodie</au><au>BESSETTE, Barbara</au><au>MATHONNET, Muriel</au><au>LALLOUE, Fabrice</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>P75 neurotrophin receptor is sequestered in the Golgi apparatus of the U-87 MG human glioblastoma cell line</atitle><jtitle>International journal of oncology</jtitle><addtitle>Int J Oncol</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>38</volume><issue>2</issue><spage>391</spage><epage>399</epage><pages>391-399</pages><issn>1019-6439</issn><eissn>1791-2423</eissn><abstract>The P75 neurotrophin receptor (p75NTR) is a cell surface receptor that can induce apoptosis in many cell types. This receptor plays a major role in the development of the central nervous system and is expressed in some adult brain cells. Its implication in cell apoptosis or survival is probably of major importance in cellular homeostasis and thus p75NTR could be implicated in tumor resistance to death. In this study, we investigated the intracellular expression of p75NTR in a human glioblastoma cell line. Detection of p75NTR receptor in Golgi apparatus by immunofluorescence microscopy, or after Golgi apparatus extraction, could be correlated with a decrease of cell apoptosis leading cells to become tumorous. This hypothesis is supported by a loss of ligand-induced apoptosis in this cell line. 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| identifier | ISSN: 1019-6439 |
| ispartof | International journal of oncology, 2011-02, Vol.38 (2), p.391-399 |
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| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Apoptosis Biological and medical sciences Blotting, Western Brain Neoplasms Brain Neoplasms - metabolism Brain Neoplasms - pathology Cancer Cellular Biology Enzyme-Linked Immunosorbent Assay Flow Cytometry Fluorescent Antibody Technique Glioblastoma Glioblastoma - metabolism Glioblastoma - pathology Glycoside Hydrolases Glycoside Hydrolases - metabolism Golgi Apparatus Golgi Apparatus - metabolism Humans Immunoenzyme Techniques Life Sciences Medical sciences Membrane Microdomains Membrane Microdomains - metabolism Neurobiology Neurology Neurons and Cognition Receptor, Nerve Growth Factor Receptor, Nerve Growth Factor - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger RNA, Messenger - genetics Subcellular Fractions Tumor Cells, Cultured Tumors Tumors of the nervous system. Phacomatoses |
| title | P75 neurotrophin receptor is sequestered in the Golgi apparatus of the U-87 MG human glioblastoma cell line |
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