The added value of rabies virus as a retrograde tracer when combined with dual anterograde tract-tracing
Rabies virus (RV) has widely been used as a trans-synaptic retrograde tracer to analyze chains of connected neurons. The use of antibodies directed against the viral nucleoprotein enables viral nucleocapsids to be visualized within the cell soma, as well as within the thickest main dendrites. Howeve...
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| Veröffentlicht in: | Journal of neuroscience methods 2010-12, Vol.194 (1), p.21-27 |
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| creator | López, Iciar P. Salin, Pascal Kachidian, Philippe Barroso-Chinea, Pedro Rico, Alberto J. Gómez-Bautista, Virginia Conte-Perales, Lorena Coulon, Patrice Goff, Lydia Kerkerian-Le Lanciego, José L. |
| description | Rabies virus (RV) has widely been used as a trans-synaptic retrograde tracer to analyze chains of connected neurons. The use of antibodies directed against the viral nucleoprotein enables viral nucleocapsids to be visualized within the cell soma, as well as within the thickest main dendrites. However, through this approach it is often difficult to accurately define post-synaptic elements (thin dendrites and/or dendritic spines). This limitation can now easily been circumvented by taking advantage of antibodies directed against a soluble viral phosphoprotein that spreads throughout the cytoplasm of the infected neuron, thereby producing Golgi-like immunofluorescent labeling of first-order projection neurons that are infected with RV. Furthermore, when combined with anterograde tracers such as
Phaseolus vulgaris-leucoagglutinin (PHA-L) and biotinylated dextran amine (BDA), this procedure to detect RV facilitates the accurate visualization of both the pre- and post-synaptic elements. Finally, this method of viral detection is sufficiently sensitive to detect weakly labeled second-order neurons, which can then be further characterized neurochemically. Several examples are provided to illustrate why retrograde trans-synaptic tracing using RV can be regarded as an important breakthrough in the analysis of brain circuits, providing an unprecedented level of resolution. |
| doi_str_mv | 10.1016/j.jneumeth.2010.01.015 |
| format | Article |
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Phaseolus vulgaris-leucoagglutinin (PHA-L) and biotinylated dextran amine (BDA), this procedure to detect RV facilitates the accurate visualization of both the pre- and post-synaptic elements. Finally, this method of viral detection is sufficiently sensitive to detect weakly labeled second-order neurons, which can then be further characterized neurochemically. Several examples are provided to illustrate why retrograde trans-synaptic tracing using RV can be regarded as an important breakthrough in the analysis of brain circuits, providing an unprecedented level of resolution.</description><identifier>ISSN: 0165-0270</identifier><identifier>EISSN: 1872-678X</identifier><identifier>DOI: 10.1016/j.jneumeth.2010.01.015</identifier><identifier>PMID: 20096304</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antibodies ; BDA ; Biotin - analogs & derivatives ; Cellular Biology ; Cerebral Cortex - anatomy & histology ; Confocal microscope ; Corpus Striatum - anatomy & histology ; Dendritic spines ; Dextrans ; Fluorescent Dyes ; Image Processing, Computer-Assisted ; Immunohistochemistry ; Interneurons - physiology ; Life Sciences ; Male ; Microscopy, Confocal ; Neuroanatomy - methods ; Neuronal Tract-Tracers ; Neurons - physiology ; PHA-L ; Phaseolus ; Phosphoproteins - immunology ; Phytohemagglutinins ; Rabies virus ; Rabies virus - physiology ; Rats ; Rats, Wistar ; Synapses - physiology ; Trans-synaptic tracing</subject><ispartof>Journal of neuroscience methods, 2010-12, Vol.194 (1), p.21-27</ispartof><rights>2010 Elsevier B.V.</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c433t-e80575428e09b3e9268a0d9907d23d60f55a400e68d4e69ad74b941a851fc8443</citedby><cites>FETCH-LOGICAL-c433t-e80575428e09b3e9268a0d9907d23d60f55a400e68d4e69ad74b941a851fc8443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165027010000373$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20096304$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00555179$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>López, Iciar P.</creatorcontrib><creatorcontrib>Salin, Pascal</creatorcontrib><creatorcontrib>Kachidian, Philippe</creatorcontrib><creatorcontrib>Barroso-Chinea, Pedro</creatorcontrib><creatorcontrib>Rico, Alberto J.</creatorcontrib><creatorcontrib>Gómez-Bautista, Virginia</creatorcontrib><creatorcontrib>Conte-Perales, Lorena</creatorcontrib><creatorcontrib>Coulon, Patrice</creatorcontrib><creatorcontrib>Goff, Lydia Kerkerian-Le</creatorcontrib><creatorcontrib>Lanciego, José L.</creatorcontrib><title>The added value of rabies virus as a retrograde tracer when combined with dual anterograde tract-tracing</title><title>Journal of neuroscience methods</title><addtitle>J Neurosci Methods</addtitle><description>Rabies virus (RV) has widely been used as a trans-synaptic retrograde tracer to analyze chains of connected neurons. The use of antibodies directed against the viral nucleoprotein enables viral nucleocapsids to be visualized within the cell soma, as well as within the thickest main dendrites. However, through this approach it is often difficult to accurately define post-synaptic elements (thin dendrites and/or dendritic spines). This limitation can now easily been circumvented by taking advantage of antibodies directed against a soluble viral phosphoprotein that spreads throughout the cytoplasm of the infected neuron, thereby producing Golgi-like immunofluorescent labeling of first-order projection neurons that are infected with RV. Furthermore, when combined with anterograde tracers such as
Phaseolus vulgaris-leucoagglutinin (PHA-L) and biotinylated dextran amine (BDA), this procedure to detect RV facilitates the accurate visualization of both the pre- and post-synaptic elements. Finally, this method of viral detection is sufficiently sensitive to detect weakly labeled second-order neurons, which can then be further characterized neurochemically. Several examples are provided to illustrate why retrograde trans-synaptic tracing using RV can be regarded as an important breakthrough in the analysis of brain circuits, providing an unprecedented level of resolution.</description><subject>Animals</subject><subject>Antibodies</subject><subject>BDA</subject><subject>Biotin - analogs & derivatives</subject><subject>Cellular Biology</subject><subject>Cerebral Cortex - anatomy & histology</subject><subject>Confocal microscope</subject><subject>Corpus Striatum - anatomy & histology</subject><subject>Dendritic spines</subject><subject>Dextrans</subject><subject>Fluorescent Dyes</subject><subject>Image Processing, Computer-Assisted</subject><subject>Immunohistochemistry</subject><subject>Interneurons - physiology</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Microscopy, Confocal</subject><subject>Neuroanatomy - methods</subject><subject>Neuronal Tract-Tracers</subject><subject>Neurons - physiology</subject><subject>PHA-L</subject><subject>Phaseolus</subject><subject>Phosphoproteins - immunology</subject><subject>Phytohemagglutinins</subject><subject>Rabies virus</subject><subject>Rabies virus - physiology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Synapses - physiology</subject><subject>Trans-synaptic tracing</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9r3DAQxUVpaTZpv0LQrfTg7UiWZOnWENqksNBLCr0JWRrHWvwnlewN_fbVsknoLfAYwfB7mmEeIZcMtgyY-rLf7idcR1z6LYfSBFYk35AN0w2vVKN_vyWbAsoKeANn5DznPQAIA-o9OeMARtUgNqS_65G6EDDQgxtWpHNHk2sjZnqIac3UFdGES5rvkwtIl-Q8JvrY40T9PLZxKtbHuPQ0rG6gblrwf3SpjjVO9x_Iu84NGT8-vRfk1_dvd9e31e7nzY_rq13lRV0vFWqQjRRcI5i2RsOVdhCMgSbwOijopHQCAJUOApVxoRGtEcxpyTqvhagvyOfTv70b7EOKo0t_7eyivb3a2WMPQErJGnNghf10Yh_S_GfFvNgxZo_D4Cac12x1ozg3tYbXSSalUKLWhVQn0qc554TdyxIM7DE6u7fP0dljdBZYkSzGy6cRaztieLE9Z1WArycAy_kOEZPNPuLkMcSEfrFhjq_N-AcD2qyZ</recordid><startdate>20101215</startdate><enddate>20101215</enddate><creator>López, Iciar P.</creator><creator>Salin, Pascal</creator><creator>Kachidian, Philippe</creator><creator>Barroso-Chinea, Pedro</creator><creator>Rico, Alberto J.</creator><creator>Gómez-Bautista, Virginia</creator><creator>Conte-Perales, Lorena</creator><creator>Coulon, Patrice</creator><creator>Goff, Lydia Kerkerian-Le</creator><creator>Lanciego, José L.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope><scope>7U9</scope><scope>H94</scope><scope>1XC</scope></search><sort><creationdate>20101215</creationdate><title>The added value of rabies virus as a retrograde tracer when combined with dual anterograde tract-tracing</title><author>López, Iciar P. ; Salin, Pascal ; Kachidian, Philippe ; Barroso-Chinea, Pedro ; Rico, Alberto J. ; Gómez-Bautista, Virginia ; Conte-Perales, Lorena ; Coulon, Patrice ; Goff, Lydia Kerkerian-Le ; Lanciego, José L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-e80575428e09b3e9268a0d9907d23d60f55a400e68d4e69ad74b941a851fc8443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>BDA</topic><topic>Biotin - analogs & derivatives</topic><topic>Cellular Biology</topic><topic>Cerebral Cortex - anatomy & histology</topic><topic>Confocal microscope</topic><topic>Corpus Striatum - anatomy & histology</topic><topic>Dendritic spines</topic><topic>Dextrans</topic><topic>Fluorescent Dyes</topic><topic>Image Processing, Computer-Assisted</topic><topic>Immunohistochemistry</topic><topic>Interneurons - physiology</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Microscopy, Confocal</topic><topic>Neuroanatomy - methods</topic><topic>Neuronal Tract-Tracers</topic><topic>Neurons - physiology</topic><topic>PHA-L</topic><topic>Phaseolus</topic><topic>Phosphoproteins - immunology</topic><topic>Phytohemagglutinins</topic><topic>Rabies virus</topic><topic>Rabies virus - physiology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Synapses - physiology</topic><topic>Trans-synaptic tracing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>López, Iciar P.</creatorcontrib><creatorcontrib>Salin, Pascal</creatorcontrib><creatorcontrib>Kachidian, Philippe</creatorcontrib><creatorcontrib>Barroso-Chinea, Pedro</creatorcontrib><creatorcontrib>Rico, Alberto J.</creatorcontrib><creatorcontrib>Gómez-Bautista, Virginia</creatorcontrib><creatorcontrib>Conte-Perales, Lorena</creatorcontrib><creatorcontrib>Coulon, Patrice</creatorcontrib><creatorcontrib>Goff, Lydia Kerkerian-Le</creatorcontrib><creatorcontrib>Lanciego, José L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of neuroscience methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>López, Iciar P.</au><au>Salin, Pascal</au><au>Kachidian, Philippe</au><au>Barroso-Chinea, Pedro</au><au>Rico, Alberto J.</au><au>Gómez-Bautista, Virginia</au><au>Conte-Perales, Lorena</au><au>Coulon, Patrice</au><au>Goff, Lydia Kerkerian-Le</au><au>Lanciego, José L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The added value of rabies virus as a retrograde tracer when combined with dual anterograde tract-tracing</atitle><jtitle>Journal of neuroscience methods</jtitle><addtitle>J Neurosci Methods</addtitle><date>2010-12-15</date><risdate>2010</risdate><volume>194</volume><issue>1</issue><spage>21</spage><epage>27</epage><pages>21-27</pages><issn>0165-0270</issn><eissn>1872-678X</eissn><abstract>Rabies virus (RV) has widely been used as a trans-synaptic retrograde tracer to analyze chains of connected neurons. The use of antibodies directed against the viral nucleoprotein enables viral nucleocapsids to be visualized within the cell soma, as well as within the thickest main dendrites. However, through this approach it is often difficult to accurately define post-synaptic elements (thin dendrites and/or dendritic spines). This limitation can now easily been circumvented by taking advantage of antibodies directed against a soluble viral phosphoprotein that spreads throughout the cytoplasm of the infected neuron, thereby producing Golgi-like immunofluorescent labeling of first-order projection neurons that are infected with RV. Furthermore, when combined with anterograde tracers such as
Phaseolus vulgaris-leucoagglutinin (PHA-L) and biotinylated dextran amine (BDA), this procedure to detect RV facilitates the accurate visualization of both the pre- and post-synaptic elements. Finally, this method of viral detection is sufficiently sensitive to detect weakly labeled second-order neurons, which can then be further characterized neurochemically. Several examples are provided to illustrate why retrograde trans-synaptic tracing using RV can be regarded as an important breakthrough in the analysis of brain circuits, providing an unprecedented level of resolution.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>20096304</pmid><doi>10.1016/j.jneumeth.2010.01.015</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of neuroscience methods, 2010-12, Vol.194 (1), p.21-27 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibodies BDA Biotin - analogs & derivatives Cellular Biology Cerebral Cortex - anatomy & histology Confocal microscope Corpus Striatum - anatomy & histology Dendritic spines Dextrans Fluorescent Dyes Image Processing, Computer-Assisted Immunohistochemistry Interneurons - physiology Life Sciences Male Microscopy, Confocal Neuroanatomy - methods Neuronal Tract-Tracers Neurons - physiology PHA-L Phaseolus Phosphoproteins - immunology Phytohemagglutinins Rabies virus Rabies virus - physiology Rats Rats, Wistar Synapses - physiology Trans-synaptic tracing |
| title | The added value of rabies virus as a retrograde tracer when combined with dual anterograde tract-tracing |
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