Real-time PCR-based determination of gene copy numbers in Pichia pastoris

Pichia pastoris is a preferred host for heterologous protein production. Expression cassettes are usually integrated into the genome of this methylotrophic yeast. This manuscript describes a method for fast and reliable gene copy number determinations for P. pastoris expression strains. We believe t...

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Veröffentlicht in:	Biotechnology journal 2010-04, Vol.5 (4), p.413-420
Hauptverfasser:	Abad, Sandra, Kitz, Kerstin, Hörmann, Astrid, Schreiner, Ulrike, Hartner, Franz S., Glieder, Anton
Format:	Artikel
Sprache:	eng
Schlagworte:	CalB Chromosome Mapping - methods Expression cassettes Gene Dosage - genetics Genome, Fungal - genetics Methods Pichia - genetics Pichia pastoris Real-time PCR Reverse Transcriptase Polymerase Chain Reaction - methods
Online-Zugang:	Volltext
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