Impact of Roundup on the marine microbial community, as shown by an in situ microcosm experiment
The effects of the herbicide Roundup ® (glyphosate) on natural marine microbial communities were assessed in a 7-day field experiment using microcosms. Bottles were maintained underwater at 6 m depth, and 10% of their water content was changed every other day. The comparison of control microcosms an...
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| Veröffentlicht in: | Aquatic toxicology 2008-09, Vol.89 (4), p.232-241 |
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| creator | Stachowski-Haberkorn, Sabine Becker, Beatriz Marie, Dominique Haberkorn, Hansy Coroller, Louis de la Broise, Denis |
| description | The effects of the herbicide Roundup
® (glyphosate) on natural marine microbial communities were assessed in a 7-day field experiment using microcosms. Bottles were maintained underwater at 6
m depth, and 10% of their water content was changed every other day.
The comparison of control microcosms and surrounding surface water showed that the microcosm system tested here can be considered as representative of the natural surrounding environment. A temporal temperature gradient gel electrophoresis (TTGE) was run on 16S and 18S rDNA-amplified extracts from the whole microbial community. Cluster analysis of the 16S gel showed differences between control and treatment fingerprints for Roundup at 1
μg
L
−1 (ANOSIM,
p
=
0.055;
R
=
0.53), and 10
μg
L
−1 (ANOSIM,
p
=
0.086;
R
=
0.40). Flow cytometry analysis revealed a significant increase in the prasinophyte-like population when Roundup concentration was increased to 10
μg
L
−1.
This study demonstrates that a disturbance was caused to the marine microbial community exposed to 1
μg
L
−1 Roundup concentration, a value typical of those reported in coastal waters during a run-off event. |
| doi_str_mv | 10.1016/j.aquatox.2008.07.004 |
| format | Article |
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® (glyphosate) on natural marine microbial communities were assessed in a 7-day field experiment using microcosms. Bottles were maintained underwater at 6
m depth, and 10% of their water content was changed every other day.
The comparison of control microcosms and surrounding surface water showed that the microcosm system tested here can be considered as representative of the natural surrounding environment. A temporal temperature gradient gel electrophoresis (TTGE) was run on 16S and 18S rDNA-amplified extracts from the whole microbial community. Cluster analysis of the 16S gel showed differences between control and treatment fingerprints for Roundup at 1
μg
L
−1 (ANOSIM,
p
=
0.055;
R
=
0.53), and 10
μg
L
−1 (ANOSIM,
p
=
0.086;
R
=
0.40). Flow cytometry analysis revealed a significant increase in the prasinophyte-like population when Roundup concentration was increased to 10
μg
L
−1.
This study demonstrates that a disturbance was caused to the marine microbial community exposed to 1
μg
L
−1 Roundup concentration, a value typical of those reported in coastal waters during a run-off event.</description><identifier>ISSN: 0166-445X</identifier><identifier>EISSN: 1879-1514</identifier><identifier>DOI: 10.1016/j.aquatox.2008.07.004</identifier><identifier>PMID: 18760491</identifier><identifier>CODEN: AQTODG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animal and plant ecology ; Animal, plant and microbial ecology ; Animals ; Applied ecology ; Biological and medical sciences ; chlorophyll ; Chlorophyll - analysis ; Chlorophyll - metabolism ; Chlorophyll A ; coastal water ; community structure ; Diatoms - drug effects ; Diatoms - growth & development ; Diatoms - metabolism ; Dinoflagellida - drug effects ; Dinoflagellida - growth & development ; Dinoflagellida - metabolism ; DNA Fingerprinting - methods ; Ecology, environment ; Ecotoxicology, biological effects of pollution ; Electrophoresis, Polyacrylamide Gel ; eukaryotic cells ; Flow Cytometry ; Food and Nutrition ; Fundamental and applied biological sciences. Psychology ; General aspects ; Glycine - analogs & derivatives ; Glycine - toxicity ; Glyphosate ; Herbicides - toxicity ; Life Sciences ; Marine microbial communities ; Microbiology and Parasitology ; Microcosm ; microorganisms ; phytoplankton ; Phytoplankton - drug effects ; Phytoplankton - growth & development ; Phytoplankton - metabolism ; Polymerase Chain Reaction ; prokaryotic cells ; Sea water ecosystems ; Seawater ; Synechococcus - drug effects ; Synechococcus - growth & development ; Synechococcus - metabolism ; Synecology ; toxicity ; TTGE ; Water Microbiology ; Water Pollutants, Chemical - toxicity ; water pollution</subject><ispartof>Aquatic toxicology, 2008-09, Vol.89 (4), p.232-241</ispartof><rights>2008 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c560t-ed7999ddaff6764b02ce69cbf2c70e186dba991bdc6cb9918f4a2e45d43cfd933</citedby><orcidid>0000-0001-7350-9471</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aquatox.2008.07.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20714559$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18760491$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.univ-brest.fr/hal-00552104$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Stachowski-Haberkorn, Sabine</creatorcontrib><creatorcontrib>Becker, Beatriz</creatorcontrib><creatorcontrib>Marie, Dominique</creatorcontrib><creatorcontrib>Haberkorn, Hansy</creatorcontrib><creatorcontrib>Coroller, Louis</creatorcontrib><creatorcontrib>de la Broise, Denis</creatorcontrib><title>Impact of Roundup on the marine microbial community, as shown by an in situ microcosm experiment</title><title>Aquatic toxicology</title><addtitle>Aquat Toxicol</addtitle><description>The effects of the herbicide Roundup
® (glyphosate) on natural marine microbial communities were assessed in a 7-day field experiment using microcosms. Bottles were maintained underwater at 6
m depth, and 10% of their water content was changed every other day.
The comparison of control microcosms and surrounding surface water showed that the microcosm system tested here can be considered as representative of the natural surrounding environment. A temporal temperature gradient gel electrophoresis (TTGE) was run on 16S and 18S rDNA-amplified extracts from the whole microbial community. Cluster analysis of the 16S gel showed differences between control and treatment fingerprints for Roundup at 1
μg
L
−1 (ANOSIM,
p
=
0.055;
R
=
0.53), and 10
μg
L
−1 (ANOSIM,
p
=
0.086;
R
=
0.40). Flow cytometry analysis revealed a significant increase in the prasinophyte-like population when Roundup concentration was increased to 10
μg
L
−1.
This study demonstrates that a disturbance was caused to the marine microbial community exposed to 1
μg
L
−1 Roundup concentration, a value typical of those reported in coastal waters during a run-off event.</description><subject>Animal and plant ecology</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Applied ecology</subject><subject>Biological and medical sciences</subject><subject>chlorophyll</subject><subject>Chlorophyll - analysis</subject><subject>Chlorophyll - metabolism</subject><subject>Chlorophyll A</subject><subject>coastal water</subject><subject>community structure</subject><subject>Diatoms - drug effects</subject><subject>Diatoms - growth & development</subject><subject>Diatoms - metabolism</subject><subject>Dinoflagellida - drug effects</subject><subject>Dinoflagellida - growth & development</subject><subject>Dinoflagellida - metabolism</subject><subject>DNA Fingerprinting - methods</subject><subject>Ecology, environment</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>eukaryotic cells</subject><subject>Flow Cytometry</subject><subject>Food and Nutrition</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Glycine - analogs & derivatives</subject><subject>Glycine - toxicity</subject><subject>Glyphosate</subject><subject>Herbicides - toxicity</subject><subject>Life Sciences</subject><subject>Marine microbial communities</subject><subject>Microbiology and Parasitology</subject><subject>Microcosm</subject><subject>microorganisms</subject><subject>phytoplankton</subject><subject>Phytoplankton - drug effects</subject><subject>Phytoplankton - growth & development</subject><subject>Phytoplankton - metabolism</subject><subject>Polymerase Chain Reaction</subject><subject>prokaryotic cells</subject><subject>Sea water ecosystems</subject><subject>Seawater</subject><subject>Synechococcus - drug effects</subject><subject>Synechococcus - growth & development</subject><subject>Synechococcus - metabolism</subject><subject>Synecology</subject><subject>toxicity</subject><subject>TTGE</subject><subject>Water Microbiology</subject><subject>Water Pollutants, Chemical - toxicity</subject><subject>water pollution</subject><issn>0166-445X</issn><issn>1879-1514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0VFr1TAUB_AiirtOP4KaFwXB1pM2SZunMcbmBhcEdeBbTJPUm0ubdEk7d7-9ubTMx-XlhPBLziH_LHuLocCA2Zd9Ie9mOfmHogRoCqgLAPIs2-Cm5jmmmDzPNsmxnBD66yR7FeMe0ioJf5mdJMSAcLzJft8Mo1QT8h367men5xF5h6adQYMM1qViVfCtlT1SfhhmZ6fDZyQjijv_16H2gKRD1qFop3mxyscBmYfRBDsYN73OXnSyj-bNWk-z26vLnxfX-fbb15uL822uKIMpN7rmnGstu47VjLRQKsO4artS1WBww3QrOcetVky1adN0RJaGUE0q1WleVafZp-XdnezFmHrLcBBeWnF9vhXHMwBKSwzkHif7cbFj8HeziZMYbFSm76Uzfo4Ccwa4rNjTkDRNBfT4Il1g-oAYg-keR8AgjnmJvVjzEse8BNRpIJLuvVsbzO1g9P9ba0AJfFiBjEr2XZBO2fjoSqgxoZQn935xnfRC_gnJ3P4oAVeAaZV-tknibBEmpXBvTRBRWeOU0TYYNQnt7RPD_gNT6cAv</recordid><startdate>20080929</startdate><enddate>20080929</enddate><creator>Stachowski-Haberkorn, Sabine</creator><creator>Becker, Beatriz</creator><creator>Marie, Dominique</creator><creator>Haberkorn, Hansy</creator><creator>Coroller, Louis</creator><creator>de la Broise, Denis</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier Science</general><general>Elsevier Science</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7QH</scope><scope>7T7</scope><scope>7TN</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H97</scope><scope>L.G</scope><scope>P64</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0001-7350-9471</orcidid></search><sort><creationdate>20080929</creationdate><title>Impact of Roundup on the marine microbial community, as shown by an in situ microcosm experiment</title><author>Stachowski-Haberkorn, Sabine ; Becker, Beatriz ; Marie, Dominique ; Haberkorn, Hansy ; Coroller, Louis ; de la Broise, Denis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c560t-ed7999ddaff6764b02ce69cbf2c70e186dba991bdc6cb9918f4a2e45d43cfd933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animal and plant ecology</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Applied ecology</topic><topic>Biological and medical sciences</topic><topic>chlorophyll</topic><topic>Chlorophyll - analysis</topic><topic>Chlorophyll - metabolism</topic><topic>Chlorophyll A</topic><topic>coastal water</topic><topic>community structure</topic><topic>Diatoms - drug effects</topic><topic>Diatoms - growth & development</topic><topic>Diatoms - metabolism</topic><topic>Dinoflagellida - drug effects</topic><topic>Dinoflagellida - growth & development</topic><topic>Dinoflagellida - metabolism</topic><topic>DNA Fingerprinting - methods</topic><topic>Ecology, environment</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>eukaryotic cells</topic><topic>Flow Cytometry</topic><topic>Food and Nutrition</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Glycine - analogs & derivatives</topic><topic>Glycine - toxicity</topic><topic>Glyphosate</topic><topic>Herbicides - toxicity</topic><topic>Life Sciences</topic><topic>Marine microbial communities</topic><topic>Microbiology and Parasitology</topic><topic>Microcosm</topic><topic>microorganisms</topic><topic>phytoplankton</topic><topic>Phytoplankton - drug effects</topic><topic>Phytoplankton - growth & development</topic><topic>Phytoplankton - metabolism</topic><topic>Polymerase Chain Reaction</topic><topic>prokaryotic cells</topic><topic>Sea water ecosystems</topic><topic>Seawater</topic><topic>Synechococcus - drug effects</topic><topic>Synechococcus - growth & development</topic><topic>Synechococcus - metabolism</topic><topic>Synecology</topic><topic>toxicity</topic><topic>TTGE</topic><topic>Water Microbiology</topic><topic>Water Pollutants, Chemical - toxicity</topic><topic>water pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stachowski-Haberkorn, Sabine</creatorcontrib><creatorcontrib>Becker, Beatriz</creatorcontrib><creatorcontrib>Marie, Dominique</creatorcontrib><creatorcontrib>Haberkorn, Hansy</creatorcontrib><creatorcontrib>Coroller, Louis</creatorcontrib><creatorcontrib>de la Broise, Denis</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Aqualine</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oceanic Abstracts</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Aquatic toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stachowski-Haberkorn, Sabine</au><au>Becker, Beatriz</au><au>Marie, Dominique</au><au>Haberkorn, Hansy</au><au>Coroller, Louis</au><au>de la Broise, Denis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impact of Roundup on the marine microbial community, as shown by an in situ microcosm experiment</atitle><jtitle>Aquatic toxicology</jtitle><addtitle>Aquat Toxicol</addtitle><date>2008-09-29</date><risdate>2008</risdate><volume>89</volume><issue>4</issue><spage>232</spage><epage>241</epage><pages>232-241</pages><issn>0166-445X</issn><eissn>1879-1514</eissn><coden>AQTODG</coden><abstract>The effects of the herbicide Roundup
® (glyphosate) on natural marine microbial communities were assessed in a 7-day field experiment using microcosms. Bottles were maintained underwater at 6
m depth, and 10% of their water content was changed every other day.
The comparison of control microcosms and surrounding surface water showed that the microcosm system tested here can be considered as representative of the natural surrounding environment. A temporal temperature gradient gel electrophoresis (TTGE) was run on 16S and 18S rDNA-amplified extracts from the whole microbial community. Cluster analysis of the 16S gel showed differences between control and treatment fingerprints for Roundup at 1
μg
L
−1 (ANOSIM,
p
=
0.055;
R
=
0.53), and 10
μg
L
−1 (ANOSIM,
p
=
0.086;
R
=
0.40). Flow cytometry analysis revealed a significant increase in the prasinophyte-like population when Roundup concentration was increased to 10
μg
L
−1.
This study demonstrates that a disturbance was caused to the marine microbial community exposed to 1
μg
L
−1 Roundup concentration, a value typical of those reported in coastal waters during a run-off event.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>18760491</pmid><doi>10.1016/j.aquatox.2008.07.004</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-7350-9471</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0166-445X |
| ispartof | Aquatic toxicology, 2008-09, Vol.89 (4), p.232-241 |
| issn | 0166-445X 1879-1514 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_00552104v1 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animal and plant ecology Animal, plant and microbial ecology Animals Applied ecology Biological and medical sciences chlorophyll Chlorophyll - analysis Chlorophyll - metabolism Chlorophyll A coastal water community structure Diatoms - drug effects Diatoms - growth & development Diatoms - metabolism Dinoflagellida - drug effects Dinoflagellida - growth & development Dinoflagellida - metabolism DNA Fingerprinting - methods Ecology, environment Ecotoxicology, biological effects of pollution Electrophoresis, Polyacrylamide Gel eukaryotic cells Flow Cytometry Food and Nutrition Fundamental and applied biological sciences. Psychology General aspects Glycine - analogs & derivatives Glycine - toxicity Glyphosate Herbicides - toxicity Life Sciences Marine microbial communities Microbiology and Parasitology Microcosm microorganisms phytoplankton Phytoplankton - drug effects Phytoplankton - growth & development Phytoplankton - metabolism Polymerase Chain Reaction prokaryotic cells Sea water ecosystems Seawater Synechococcus - drug effects Synechococcus - growth & development Synechococcus - metabolism Synecology toxicity TTGE Water Microbiology Water Pollutants, Chemical - toxicity water pollution |
| title | Impact of Roundup on the marine microbial community, as shown by an in situ microcosm experiment |
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