Genomic and phenotypic changes of Campylobacter jejuni strains after passage of the chicken gut
The ability to colonize the chicken gut was determined for 17 Campylobacter jejuni strains of human and bovine origin. The level of colonization varied according to the strain used for experimental infection. Two Campylobacter isolates from patients suffering from gastroenteritis were found in the g...
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| Veröffentlicht in: | Veterinary microbiology 2009-04, Vol.136 (1), p.121-129 |
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| creator | Hänel, I. Borrmann, E. Müller, J. Müller, W. Pauly, B. Liebler-Tenorio, E.M. Schulze, F. |
| description | The ability to colonize the chicken gut was determined for 17
Campylobacter jejuni strains of human and bovine origin. The level of colonization varied according to the strain used for experimental infection. Two
Campylobacter isolates from patients suffering from gastroenteritis were found in the group of non-colonizing strains, suggesting that other reservoirs as poultry are also important sources of human
Campylobacter infections. Bovine
Campylobacter isolates can also effective colonize the chicken intestine and may be a source for poultry infection. The invasion ability of the strains as determined in the cell culture model using Caco-2 cells correlates with their colonization capacity in the chicken gut. The genomic and phenotypic stability of the selected strains were evaluated by analysis of their pulsed-field gel electrophoresis (PFGE) patterns,
flaA-typing and
in vitro determination of motility, adhesion and invasion abilities after colonizing chickens for up to 21 days. Changes were identified in
flaA-types of six isolates and three isolates from chicken showed different patterns by PFGE using
SmaI or KpnI as restriction enzymes. One isolate showed phenotypic differences after in vivo passage which were seen in enhancement of adherence to eukaryotic cells, decrease of motility and changes in morphology. These phenotypic changes were not associated with the observed genomic instabilities. |
| doi_str_mv | 10.1016/j.vetmic.2008.10.018 |
| format | Article |
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Campylobacter jejuni strains of human and bovine origin. The level of colonization varied according to the strain used for experimental infection. Two
Campylobacter isolates from patients suffering from gastroenteritis were found in the group of non-colonizing strains, suggesting that other reservoirs as poultry are also important sources of human
Campylobacter infections. Bovine
Campylobacter isolates can also effective colonize the chicken intestine and may be a source for poultry infection. The invasion ability of the strains as determined in the cell culture model using Caco-2 cells correlates with their colonization capacity in the chicken gut. The genomic and phenotypic stability of the selected strains were evaluated by analysis of their pulsed-field gel electrophoresis (PFGE) patterns,
flaA-typing and
in vitro determination of motility, adhesion and invasion abilities after colonizing chickens for up to 21 days. Changes were identified in
flaA-types of six isolates and three isolates from chicken showed different patterns by PFGE using
SmaI or KpnI as restriction enzymes. One isolate showed phenotypic differences after in vivo passage which were seen in enhancement of adherence to eukaryotic cells, decrease of motility and changes in morphology. These phenotypic changes were not associated with the observed genomic instabilities.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2008.10.018</identifier><identifier>PMID: 19058931</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Bacterial Adhesion ; bacterial colonization ; Bacteriology ; Biological and medical sciences ; Caco-2 Cells ; Campylobacter Infections - microbiology ; Campylobacter Infections - veterinary ; Campylobacter jejuni ; Campylobacter jejuni - genetics ; Campylobacter jejuni - growth & development ; Campylobacter jejuni - pathogenicity ; Campylobacter jejuni - physiology ; campylobacteriosis ; Cattle ; cell invasion ; Cell Movement - immunology ; Chicken colonization ; Chickens ; disease reservoirs ; DNA, Bacterial - genetics ; Electrophoresis, Gel, Pulsed-Field - veterinary ; epidemiological studies ; food pathogens ; Fundamental and applied biological sciences. Psychology ; gastrointestinal system ; genetic recombination ; genomics ; human diseases ; Humans ; in vitro studies ; Intestinal Diseases - microbiology ; Intestinal Diseases - veterinary ; Invasion ; Microbiology ; Microscopy, Electron, Transmission - veterinary ; Miscellaneous ; pathotypes ; phenotype ; Polymerase Chain Reaction - veterinary ; Polymorphism, Restriction Fragment Length ; poultry diseases ; Poultry Diseases - microbiology ; Random Allocation ; risk assessment ; Specific Pathogen-Free Organisms ; strain differences ; strains ; Typing ; virulence ; Virulence Factors - genetics</subject><ispartof>Veterinary microbiology, 2009-04, Vol.136 (1), p.121-129</ispartof><rights>2008 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-952aa9eca6f24d5b142830a864b09a368961c268322f1945f8fdb319380ecd0e3</citedby><cites>FETCH-LOGICAL-c592t-952aa9eca6f24d5b142830a864b09a368961c268322f1945f8fdb319380ecd0e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S037811350800494X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21391414$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19058931$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00532525$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Hänel, I.</creatorcontrib><creatorcontrib>Borrmann, E.</creatorcontrib><creatorcontrib>Müller, J.</creatorcontrib><creatorcontrib>Müller, W.</creatorcontrib><creatorcontrib>Pauly, B.</creatorcontrib><creatorcontrib>Liebler-Tenorio, E.M.</creatorcontrib><creatorcontrib>Schulze, F.</creatorcontrib><title>Genomic and phenotypic changes of Campylobacter jejuni strains after passage of the chicken gut</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>The ability to colonize the chicken gut was determined for 17
Campylobacter jejuni strains of human and bovine origin. The level of colonization varied according to the strain used for experimental infection. Two
Campylobacter isolates from patients suffering from gastroenteritis were found in the group of non-colonizing strains, suggesting that other reservoirs as poultry are also important sources of human
Campylobacter infections. Bovine
Campylobacter isolates can also effective colonize the chicken intestine and may be a source for poultry infection. The invasion ability of the strains as determined in the cell culture model using Caco-2 cells correlates with their colonization capacity in the chicken gut. The genomic and phenotypic stability of the selected strains were evaluated by analysis of their pulsed-field gel electrophoresis (PFGE) patterns,
flaA-typing and
in vitro determination of motility, adhesion and invasion abilities after colonizing chickens for up to 21 days. Changes were identified in
flaA-types of six isolates and three isolates from chicken showed different patterns by PFGE using
SmaI or KpnI as restriction enzymes. One isolate showed phenotypic differences after in vivo passage which were seen in enhancement of adherence to eukaryotic cells, decrease of motility and changes in morphology. These phenotypic changes were not associated with the observed genomic instabilities.</description><subject>Animals</subject><subject>Bacterial Adhesion</subject><subject>bacterial colonization</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Caco-2 Cells</subject><subject>Campylobacter Infections - microbiology</subject><subject>Campylobacter Infections - veterinary</subject><subject>Campylobacter jejuni</subject><subject>Campylobacter jejuni - genetics</subject><subject>Campylobacter jejuni - growth & development</subject><subject>Campylobacter jejuni - pathogenicity</subject><subject>Campylobacter jejuni - physiology</subject><subject>campylobacteriosis</subject><subject>Cattle</subject><subject>cell invasion</subject><subject>Cell Movement - immunology</subject><subject>Chicken colonization</subject><subject>Chickens</subject><subject>disease reservoirs</subject><subject>DNA, Bacterial - genetics</subject><subject>Electrophoresis, Gel, Pulsed-Field - veterinary</subject><subject>epidemiological studies</subject><subject>food pathogens</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gastrointestinal system</subject><subject>genetic recombination</subject><subject>genomics</subject><subject>human diseases</subject><subject>Humans</subject><subject>in vitro studies</subject><subject>Intestinal Diseases - microbiology</subject><subject>Intestinal Diseases - veterinary</subject><subject>Invasion</subject><subject>Microbiology</subject><subject>Microscopy, Electron, Transmission - veterinary</subject><subject>Miscellaneous</subject><subject>pathotypes</subject><subject>phenotype</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>poultry diseases</subject><subject>Poultry Diseases - microbiology</subject><subject>Random Allocation</subject><subject>risk assessment</subject><subject>Specific Pathogen-Free Organisms</subject><subject>strain differences</subject><subject>strains</subject><subject>Typing</subject><subject>virulence</subject><subject>Virulence Factors - genetics</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90U2P0zAQBuAIgdjuwj9AkAsgDi0ef9W-IK2qZRepEgfYszV1Jq1LmgQ7rdR_j6NUy21P1oyeGY_0FsU7YAtgoL_uFycaDsEvOGMmtxYMzItiBmYp5lxJ_rKYMbE0cwChrorrlPaMMWk1e11cgWXKWAGzwt1T2-UtJbZV2e9yMZz7XPodtltKZVeXKzz056bboB8olnvaH9tQpiFiaFOJ9djsMSXc0qiHHeXh4P9QW26Pw5viVY1NoreX96Z4_H73e_UwX_-8_7G6Xc-9snyYW8URLXnUNZeV2oDkRjA0Wm6YRaGN1eC5NoLzGqxUtamrjQArDCNfMRI3xZdp7w4b18dwwHh2HQb3cLt2Y48xJbji6gTZfp5sH7u_R0qDO4TkqWmwpe6Y3FJqUFpbmeWnZ6VeMqOFshnKCfrYpRSpfroBmBvjcns3xeXGuMZujiuPvb_sP24OVP0fuuSTwccLwOSxqSO2PqQnx0FYkDAe-mFyNXYOtzGbx1-cgchfg5BaZfFtEpRTOAWKLvlAracqRPKDq7rw_K3_AM4BvNs</recordid><startdate>20090414</startdate><enddate>20090414</enddate><creator>Hänel, I.</creator><creator>Borrmann, E.</creator><creator>Müller, J.</creator><creator>Müller, W.</creator><creator>Pauly, B.</creator><creator>Liebler-Tenorio, E.M.</creator><creator>Schulze, F.</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope><scope>1XC</scope><scope>VOOES</scope></search><sort><creationdate>20090414</creationdate><title>Genomic and phenotypic changes of Campylobacter jejuni strains after passage of the chicken gut</title><author>Hänel, I. ; Borrmann, E. ; Müller, J. ; Müller, W. ; Pauly, B. ; Liebler-Tenorio, E.M. ; Schulze, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c592t-952aa9eca6f24d5b142830a864b09a368961c268322f1945f8fdb319380ecd0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Bacterial Adhesion</topic><topic>bacterial colonization</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Caco-2 Cells</topic><topic>Campylobacter Infections - microbiology</topic><topic>Campylobacter Infections - veterinary</topic><topic>Campylobacter jejuni</topic><topic>Campylobacter jejuni - genetics</topic><topic>Campylobacter jejuni - growth & development</topic><topic>Campylobacter jejuni - pathogenicity</topic><topic>Campylobacter jejuni - physiology</topic><topic>campylobacteriosis</topic><topic>Cattle</topic><topic>cell invasion</topic><topic>Cell Movement - immunology</topic><topic>Chicken colonization</topic><topic>Chickens</topic><topic>disease reservoirs</topic><topic>DNA, Bacterial - genetics</topic><topic>Electrophoresis, Gel, Pulsed-Field - veterinary</topic><topic>epidemiological studies</topic><topic>food pathogens</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gastrointestinal system</topic><topic>genetic recombination</topic><topic>genomics</topic><topic>human diseases</topic><topic>Humans</topic><topic>in vitro studies</topic><topic>Intestinal Diseases - microbiology</topic><topic>Intestinal Diseases - veterinary</topic><topic>Invasion</topic><topic>Microbiology</topic><topic>Microscopy, Electron, Transmission - veterinary</topic><topic>Miscellaneous</topic><topic>pathotypes</topic><topic>phenotype</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>poultry diseases</topic><topic>Poultry Diseases - microbiology</topic><topic>Random Allocation</topic><topic>risk assessment</topic><topic>Specific Pathogen-Free Organisms</topic><topic>strain differences</topic><topic>strains</topic><topic>Typing</topic><topic>virulence</topic><topic>Virulence Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hänel, I.</creatorcontrib><creatorcontrib>Borrmann, E.</creatorcontrib><creatorcontrib>Müller, J.</creatorcontrib><creatorcontrib>Müller, W.</creatorcontrib><creatorcontrib>Pauly, B.</creatorcontrib><creatorcontrib>Liebler-Tenorio, E.M.</creatorcontrib><creatorcontrib>Schulze, F.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hänel, I.</au><au>Borrmann, E.</au><au>Müller, J.</au><au>Müller, W.</au><au>Pauly, B.</au><au>Liebler-Tenorio, E.M.</au><au>Schulze, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic and phenotypic changes of Campylobacter jejuni strains after passage of the chicken gut</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2009-04-14</date><risdate>2009</risdate><volume>136</volume><issue>1</issue><spage>121</spage><epage>129</epage><pages>121-129</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>The ability to colonize the chicken gut was determined for 17
Campylobacter jejuni strains of human and bovine origin. The level of colonization varied according to the strain used for experimental infection. Two
Campylobacter isolates from patients suffering from gastroenteritis were found in the group of non-colonizing strains, suggesting that other reservoirs as poultry are also important sources of human
Campylobacter infections. Bovine
Campylobacter isolates can also effective colonize the chicken intestine and may be a source for poultry infection. The invasion ability of the strains as determined in the cell culture model using Caco-2 cells correlates with their colonization capacity in the chicken gut. The genomic and phenotypic stability of the selected strains were evaluated by analysis of their pulsed-field gel electrophoresis (PFGE) patterns,
flaA-typing and
in vitro determination of motility, adhesion and invasion abilities after colonizing chickens for up to 21 days. Changes were identified in
flaA-types of six isolates and three isolates from chicken showed different patterns by PFGE using
SmaI or KpnI as restriction enzymes. One isolate showed phenotypic differences after in vivo passage which were seen in enhancement of adherence to eukaryotic cells, decrease of motility and changes in morphology. These phenotypic changes were not associated with the observed genomic instabilities.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19058931</pmid><doi>10.1016/j.vetmic.2008.10.018</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1135 |
| ispartof | Veterinary microbiology, 2009-04, Vol.136 (1), p.121-129 |
| issn | 0378-1135 1873-2542 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_00532525v1 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Bacterial Adhesion bacterial colonization Bacteriology Biological and medical sciences Caco-2 Cells Campylobacter Infections - microbiology Campylobacter Infections - veterinary Campylobacter jejuni Campylobacter jejuni - genetics Campylobacter jejuni - growth & development Campylobacter jejuni - pathogenicity Campylobacter jejuni - physiology campylobacteriosis Cattle cell invasion Cell Movement - immunology Chicken colonization Chickens disease reservoirs DNA, Bacterial - genetics Electrophoresis, Gel, Pulsed-Field - veterinary epidemiological studies food pathogens Fundamental and applied biological sciences. Psychology gastrointestinal system genetic recombination genomics human diseases Humans in vitro studies Intestinal Diseases - microbiology Intestinal Diseases - veterinary Invasion Microbiology Microscopy, Electron, Transmission - veterinary Miscellaneous pathotypes phenotype Polymerase Chain Reaction - veterinary Polymorphism, Restriction Fragment Length poultry diseases Poultry Diseases - microbiology Random Allocation risk assessment Specific Pathogen-Free Organisms strain differences strains Typing virulence Virulence Factors - genetics |
| title | Genomic and phenotypic changes of Campylobacter jejuni strains after passage of the chicken gut |
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