Accelerated Achilles tendon healing by PDGF gene delivery with mesoporous silica nanoparticles
Abstract We report the ability of amino- and carboxyl-modified MCM-41 mesoporous silica nanoparticles (MSN) to deliver gene in vivo in rat Achilles tendons, despite their inefficiency to transfect primary tenocytes in culture. We show that luciferase activity lasted for at least 2 weeks in tendons i...
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| Veröffentlicht in: | Biomaterials 2010-07, Vol.31 (19), p.5237-5245 |
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| creator | Suwalski, Arnaud Dabboue, Hinda Delalande, Anthony Bensamoun, Sabine F Canon, Francis Midoux, Patrick Saillant, Gérard Klatzmann, David Salvetat, Jean-Paul Pichon, Chantal |
| description | Abstract We report the ability of amino- and carboxyl-modified MCM-41 mesoporous silica nanoparticles (MSN) to deliver gene in vivo in rat Achilles tendons, despite their inefficiency to transfect primary tenocytes in culture. We show that luciferase activity lasted for at least 2 weeks in tendons injected with these MSN and a plasmid DNA (pDNA) encoding the luciferase reporter gene. By contrast, in tendons injected with naked plasmid, the luciferase expression decreased as a function of time and became hardly detectable after 2 weeks. Interestingly, there were neither signs of inflammation nor necrosis in tendon, kidney, heart and liver of rat weekly injected with pDNA/MSN formulation during 1.5 months. Our main data concern the acceleration of Achilles tendons healing by PDGF-B gene transfer using MSN. Biomechanical properties and histological analyses clearly indicate that tendons treated with MSN and PDGF gene healed significantly faster than untreated tendons and those treated with pPDGF alone. |
| doi_str_mv | 10.1016/j.biomaterials.2010.02.077 |
| format | Article |
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We show that luciferase activity lasted for at least 2 weeks in tendons injected with these MSN and a plasmid DNA (pDNA) encoding the luciferase reporter gene. By contrast, in tendons injected with naked plasmid, the luciferase expression decreased as a function of time and became hardly detectable after 2 weeks. Interestingly, there were neither signs of inflammation nor necrosis in tendon, kidney, heart and liver of rat weekly injected with pDNA/MSN formulation during 1.5 months. Our main data concern the acceleration of Achilles tendons healing by PDGF-B gene transfer using MSN. Biomechanical properties and histological analyses clearly indicate that tendons treated with MSN and PDGF gene healed significantly faster than untreated tendons and those treated with pPDGF alone.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2010.02.077</identifier><identifier>PMID: 20334910</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Achilles Tendon - drug effects ; Achilles Tendon - pathology ; Advanced Basic Science ; Animals ; Dentistry ; Drug Carriers - administration & dosage ; Drug Carriers - chemistry ; Gene therapy ; Growth factor ; Life Sciences ; Nanoparticles - administration & dosage ; Nanoparticles - chemistry ; Nanoparticles - ultrastructure ; Non viral gene delivery ; Platelet-Derived Growth Factor - administration & dosage ; Platelet-Derived Growth Factor - chemistry ; Platelet-Derived Growth Factor - genetics ; Rats ; Rats, Wistar ; Silica nanoparticle, MCM-41 ; Silicon Dioxide - chemistry ; Tendon ; Tendon Injuries - drug therapy ; Tendon Injuries - pathology ; Transfection - methods ; Treatment Outcome</subject><ispartof>Biomaterials, 2010-07, Vol.31 (19), p.5237-5245</ispartof><rights>Elsevier Ltd</rights><rights>2010 Elsevier Ltd</rights><rights>Copyright 2010 Elsevier Ltd. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c552t-9abb261b40e424a55b4f94f4a617bfc2d7db9542b1456c52d4321e52fc0ee2cd3</citedby><cites>FETCH-LOGICAL-c552t-9abb261b40e424a55b4f94f4a617bfc2d7db9542b1456c52d4321e52fc0ee2cd3</cites><orcidid>0000-0002-0054-3422 ; 0000-0001-8280-4616 ; 0000-0003-3161-3937 ; 0000-0001-7049-9466 ; 0000-0003-4290-0173</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961210003418$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20334910$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00529475$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Suwalski, Arnaud</creatorcontrib><creatorcontrib>Dabboue, Hinda</creatorcontrib><creatorcontrib>Delalande, Anthony</creatorcontrib><creatorcontrib>Bensamoun, Sabine F</creatorcontrib><creatorcontrib>Canon, Francis</creatorcontrib><creatorcontrib>Midoux, Patrick</creatorcontrib><creatorcontrib>Saillant, Gérard</creatorcontrib><creatorcontrib>Klatzmann, David</creatorcontrib><creatorcontrib>Salvetat, Jean-Paul</creatorcontrib><creatorcontrib>Pichon, Chantal</creatorcontrib><title>Accelerated Achilles tendon healing by PDGF gene delivery with mesoporous silica nanoparticles</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract We report the ability of amino- and carboxyl-modified MCM-41 mesoporous silica nanoparticles (MSN) to deliver gene in vivo in rat Achilles tendons, despite their inefficiency to transfect primary tenocytes in culture. We show that luciferase activity lasted for at least 2 weeks in tendons injected with these MSN and a plasmid DNA (pDNA) encoding the luciferase reporter gene. By contrast, in tendons injected with naked plasmid, the luciferase expression decreased as a function of time and became hardly detectable after 2 weeks. Interestingly, there were neither signs of inflammation nor necrosis in tendon, kidney, heart and liver of rat weekly injected with pDNA/MSN formulation during 1.5 months. Our main data concern the acceleration of Achilles tendons healing by PDGF-B gene transfer using MSN. Biomechanical properties and histological analyses clearly indicate that tendons treated with MSN and PDGF gene healed significantly faster than untreated tendons and those treated with pPDGF alone.</description><subject>Achilles Tendon - drug effects</subject><subject>Achilles Tendon - pathology</subject><subject>Advanced Basic Science</subject><subject>Animals</subject><subject>Dentistry</subject><subject>Drug Carriers - administration & dosage</subject><subject>Drug Carriers - chemistry</subject><subject>Gene therapy</subject><subject>Growth factor</subject><subject>Life Sciences</subject><subject>Nanoparticles - administration & dosage</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - ultrastructure</subject><subject>Non viral gene delivery</subject><subject>Platelet-Derived Growth Factor - administration & dosage</subject><subject>Platelet-Derived Growth Factor - chemistry</subject><subject>Platelet-Derived Growth Factor - genetics</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Silica nanoparticle, MCM-41</subject><subject>Silicon Dioxide - chemistry</subject><subject>Tendon</subject><subject>Tendon Injuries - drug therapy</subject><subject>Tendon Injuries - pathology</subject><subject>Transfection - methods</subject><subject>Treatment Outcome</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk9vEzEQxVcIREPhKyCLC-KwYTxr72Y5IEUtbZEigQRcsfxntnHYrFN7E5Rvj1cpFeICJ8v2e2_G83NRvOIw58Drt5u58WGrR4pe92mOkC8A59A0j4oZXzSLUrYgHxcz4ALLtuZ4VjxLaQN5DwKfFmcIVSVaDrPi-9Ja6inmNMeWdu37nhIbaXBhYGvSvR9umTmyz5fXV-yWBmKOen-geGQ__bhmW0phF2LYJ5Z8761mgx7CTsfR25z0vHjS5R7pxf16Xny7-vD14qZcfbr-eLFclVZKHMtWG4M1NwJIoNBSGtG1ohO65o3pLLrGmVYKNFzI2kp0okJOEjsLRGhddV68OeWuda920W91PKqgvbpZrtR0BiCxFY088Kx9fdLuYrjbUxrV1qc8hF4PlN-hmhoQEPni38pKAEpcYFa-OyltDClF6h6a4KAmaGqj_oSmJmgKUGVo2fzyvszebMk9WH9TyoLLk4DyCA-eokrW02DJ-Uh2VC74_6vz_q8Ym_FmZv0POlLahH0cJg9XKRvUl-n7TL-HA0Al8jx-AWtQw6k</recordid><startdate>20100701</startdate><enddate>20100701</enddate><creator>Suwalski, Arnaud</creator><creator>Dabboue, Hinda</creator><creator>Delalande, Anthony</creator><creator>Bensamoun, Sabine F</creator><creator>Canon, Francis</creator><creator>Midoux, Patrick</creator><creator>Saillant, Gérard</creator><creator>Klatzmann, David</creator><creator>Salvetat, Jean-Paul</creator><creator>Pichon, Chantal</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0002-0054-3422</orcidid><orcidid>https://orcid.org/0000-0001-8280-4616</orcidid><orcidid>https://orcid.org/0000-0003-3161-3937</orcidid><orcidid>https://orcid.org/0000-0001-7049-9466</orcidid><orcidid>https://orcid.org/0000-0003-4290-0173</orcidid></search><sort><creationdate>20100701</creationdate><title>Accelerated Achilles tendon healing by PDGF gene delivery with mesoporous silica nanoparticles</title><author>Suwalski, Arnaud ; Dabboue, Hinda ; Delalande, Anthony ; Bensamoun, Sabine F ; Canon, Francis ; Midoux, Patrick ; Saillant, Gérard ; Klatzmann, David ; Salvetat, Jean-Paul ; Pichon, Chantal</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c552t-9abb261b40e424a55b4f94f4a617bfc2d7db9542b1456c52d4321e52fc0ee2cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Achilles Tendon - drug effects</topic><topic>Achilles Tendon - pathology</topic><topic>Advanced Basic Science</topic><topic>Animals</topic><topic>Dentistry</topic><topic>Drug Carriers - administration & dosage</topic><topic>Drug Carriers - chemistry</topic><topic>Gene therapy</topic><topic>Growth factor</topic><topic>Life Sciences</topic><topic>Nanoparticles - administration & dosage</topic><topic>Nanoparticles - chemistry</topic><topic>Nanoparticles - ultrastructure</topic><topic>Non viral gene delivery</topic><topic>Platelet-Derived Growth Factor - administration & dosage</topic><topic>Platelet-Derived Growth Factor - chemistry</topic><topic>Platelet-Derived Growth Factor - genetics</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Silica nanoparticle, MCM-41</topic><topic>Silicon Dioxide - chemistry</topic><topic>Tendon</topic><topic>Tendon Injuries - drug therapy</topic><topic>Tendon Injuries - pathology</topic><topic>Transfection - methods</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suwalski, Arnaud</creatorcontrib><creatorcontrib>Dabboue, Hinda</creatorcontrib><creatorcontrib>Delalande, Anthony</creatorcontrib><creatorcontrib>Bensamoun, Sabine F</creatorcontrib><creatorcontrib>Canon, Francis</creatorcontrib><creatorcontrib>Midoux, Patrick</creatorcontrib><creatorcontrib>Saillant, Gérard</creatorcontrib><creatorcontrib>Klatzmann, David</creatorcontrib><creatorcontrib>Salvetat, Jean-Paul</creatorcontrib><creatorcontrib>Pichon, Chantal</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suwalski, Arnaud</au><au>Dabboue, Hinda</au><au>Delalande, Anthony</au><au>Bensamoun, Sabine F</au><au>Canon, Francis</au><au>Midoux, Patrick</au><au>Saillant, Gérard</au><au>Klatzmann, David</au><au>Salvetat, Jean-Paul</au><au>Pichon, Chantal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Accelerated Achilles tendon healing by PDGF gene delivery with mesoporous silica nanoparticles</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2010-07-01</date><risdate>2010</risdate><volume>31</volume><issue>19</issue><spage>5237</spage><epage>5245</epage><pages>5237-5245</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract We report the ability of amino- and carboxyl-modified MCM-41 mesoporous silica nanoparticles (MSN) to deliver gene in vivo in rat Achilles tendons, despite their inefficiency to transfect primary tenocytes in culture. We show that luciferase activity lasted for at least 2 weeks in tendons injected with these MSN and a plasmid DNA (pDNA) encoding the luciferase reporter gene. By contrast, in tendons injected with naked plasmid, the luciferase expression decreased as a function of time and became hardly detectable after 2 weeks. Interestingly, there were neither signs of inflammation nor necrosis in tendon, kidney, heart and liver of rat weekly injected with pDNA/MSN formulation during 1.5 months. Our main data concern the acceleration of Achilles tendons healing by PDGF-B gene transfer using MSN. 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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Achilles Tendon - drug effects Achilles Tendon - pathology Advanced Basic Science Animals Dentistry Drug Carriers - administration & dosage Drug Carriers - chemistry Gene therapy Growth factor Life Sciences Nanoparticles - administration & dosage Nanoparticles - chemistry Nanoparticles - ultrastructure Non viral gene delivery Platelet-Derived Growth Factor - administration & dosage Platelet-Derived Growth Factor - chemistry Platelet-Derived Growth Factor - genetics Rats Rats, Wistar Silica nanoparticle, MCM-41 Silicon Dioxide - chemistry Tendon Tendon Injuries - drug therapy Tendon Injuries - pathology Transfection - methods Treatment Outcome |
| title | Accelerated Achilles tendon healing by PDGF gene delivery with mesoporous silica nanoparticles |
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