Induction of blood-brain barrier properties in cultured brain capillary endothelial cells: Comparison between primary glial cells and C6 cell line
The communication between glial cells and brain capillary endothelial cells is crucial for a well‐differentiated blood‐brain barrier (BBB). It has been suggested that in vitro primary glial cells (GCs) be replaced by the glial C6 cell line to standardise the model further. This study compares direct...
Gespeichert in:
| Veröffentlicht in: | Glia 2005-08, Vol.51 (3), p.187-198 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 198 |
|---|---|
| container_issue | 3 |
| container_start_page | 187 |
| container_title | Glia |
| container_volume | 51 |
| creator | Boveri, Monica Berezowski, Vincent Price, Anna Slupek, Stephanie Lenfant, Anne-Marie Benaud, Christelle Hartung, Thomas Cecchelli, Romeo Prieto, Pilar Dehouck, Marie-Pierre |
| description | The communication between glial cells and brain capillary endothelial cells is crucial for a well‐differentiated blood‐brain barrier (BBB). It has been suggested that in vitro primary glial cells (GCs) be replaced by the glial C6 cell line to standardise the model further. This study compares directly the structural and functional differentiation of bovine brain capillary endothelial cells (BBCECs) induced by co‐culture with rat primary GCs or C6 cells, for the first time. Trans‐endothelial electrical resistance (TEER) measurements showed that under no condition were C6 cells able to reproduce TEER values as high as in the presence of GCs. At the same time, permeability of the BBCECs to both radioactive sucrose and FITC‐inulin was 2.5‐fold higher when cells were co‐cultured with C6 than with GCs. Furthermore, immunocytochemistry studies showed different cell morphology and less developed tight junction pattern of BBCECs co‐cultured with C6 toward GCs. Additionally, studies on P‐glycoprotein (P‐gp) showed much lower P‐gp presence and activity in BBCECs co‐cultured with C6 than GCs. Both VEGF mRNA expression and protein content were dramatically increased when compared with GCs, suggesting that VEGF could be one of the factors responsible for higher permeability of BBB. Our results clearly indicate that, in the presence of the glial C6 cell line, BBCECs did not differentiate as well as in the co‐culture with primary GCs at both structural and functional levels. © 2005 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/glia.20189 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_00527237v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>68054432</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5019-7904e066517eade578c34535946749daae2753d8042d673a812cafa7a6b70caa3</originalsourceid><addsrcrecordid>eNqF0c1u1DAQAOAIgWgpXHgA5AtIIKWME_8k3JYVbJeuygUEN2vizLYGb7LYCaWvwRPjbZbuDU7WWN_8aCbLnnI45QDF60vv8LQAXtX3smMOdZVzXqr72TFUtci5qPlR9ijGbwA8BfphdsRlBVAX1XH2e9m1ox1c37F-zRrf923eBHQdazAER4FtQ7-lMDiKLP3a0Q9joJZNyOLWeY_hhlHX9sMVpVE8s-R9fMPm_WaLwcVUu6HhmqhLxdxmpy8PjmHXsrm6DZh3HT3OHqzRR3qyf0-yz-_ffZqf5auPi-V8tsqtBF7nugZBoJTkmrAlqStbClnKWigt6haRCi3LtgJRtEqXWPHC4ho1qkaDRSxPspdT3Sv0Zj-Y6dGZs9nK7P4AZKGLUv_kyb6YbNrGj5HiYDYu7ibGjvoxGlWBFKIs_gu5LgVXoBJ8NUEb-hgDre9G4GB2ZzW7HZnbsyb8bF91bDbUHuj-jgk83wOMFv06YGddPDhVK6kFJMcnd-083fyjpVmslrO_zfMpx8WBft3lYPhu0l61NF8uFqb6eq7O3158MIvyD-zZydQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17341606</pqid></control><display><type>article</type><title>Induction of blood-brain barrier properties in cultured brain capillary endothelial cells: Comparison between primary glial cells and C6 cell line</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Boveri, Monica ; Berezowski, Vincent ; Price, Anna ; Slupek, Stephanie ; Lenfant, Anne-Marie ; Benaud, Christelle ; Hartung, Thomas ; Cecchelli, Romeo ; Prieto, Pilar ; Dehouck, Marie-Pierre</creator><creatorcontrib>Boveri, Monica ; Berezowski, Vincent ; Price, Anna ; Slupek, Stephanie ; Lenfant, Anne-Marie ; Benaud, Christelle ; Hartung, Thomas ; Cecchelli, Romeo ; Prieto, Pilar ; Dehouck, Marie-Pierre</creatorcontrib><description>The communication between glial cells and brain capillary endothelial cells is crucial for a well‐differentiated blood‐brain barrier (BBB). It has been suggested that in vitro primary glial cells (GCs) be replaced by the glial C6 cell line to standardise the model further. This study compares directly the structural and functional differentiation of bovine brain capillary endothelial cells (BBCECs) induced by co‐culture with rat primary GCs or C6 cells, for the first time. Trans‐endothelial electrical resistance (TEER) measurements showed that under no condition were C6 cells able to reproduce TEER values as high as in the presence of GCs. At the same time, permeability of the BBCECs to both radioactive sucrose and FITC‐inulin was 2.5‐fold higher when cells were co‐cultured with C6 than with GCs. Furthermore, immunocytochemistry studies showed different cell morphology and less developed tight junction pattern of BBCECs co‐cultured with C6 toward GCs. Additionally, studies on P‐glycoprotein (P‐gp) showed much lower P‐gp presence and activity in BBCECs co‐cultured with C6 than GCs. Both VEGF mRNA expression and protein content were dramatically increased when compared with GCs, suggesting that VEGF could be one of the factors responsible for higher permeability of BBB. Our results clearly indicate that, in the presence of the glial C6 cell line, BBCECs did not differentiate as well as in the co‐culture with primary GCs at both structural and functional levels. © 2005 Wiley‐Liss, Inc.</description><identifier>ISSN: 0894-1491</identifier><identifier>EISSN: 1098-1136</identifier><identifier>DOI: 10.1002/glia.20189</identifier><identifier>PMID: 15800928</identifier><identifier>CODEN: GLIAEJ</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Animals, Newborn ; ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism ; Biochemistry, Molecular Biology ; Biological and medical sciences ; blood-brain barrier ; Blood-Brain Barrier - cytology ; Blood-Brain Barrier - metabolism ; Brain - blood supply ; Brain - physiology ; Cattle ; Cell Communication - physiology ; Cell Differentiation - physiology ; Cell Line, Tumor ; Cell Membrane Permeability - physiology ; Cells, Cultured ; Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges ; Coculture Techniques ; Electric Impedance ; endothelial cells ; Endothelial Cells - cytology ; Endothelial Cells - metabolism ; Fundamental and applied biological sciences. Psychology ; Gap Junctions - metabolism ; in vitro model ; Inulin - pharmacokinetics ; Isolated neuron and nerve. Neuroglia ; Life Sciences ; Membrane Potentials - physiology ; Neuroglia - cytology ; Neuroglia - metabolism ; primary glial cells ; Rats ; Rats, Sprague-Dawley ; RNA, Messenger - metabolism ; Sucrose - pharmacokinetics ; Up-Regulation - physiology ; Vascular Endothelial Growth Factor A - genetics ; Vascular Endothelial Growth Factor A - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>Glia, 2005-08, Vol.51 (3), p.187-198</ispartof><rights>Copyright © 2005 Wiley‐Liss, Inc.</rights><rights>2005 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5019-7904e066517eade578c34535946749daae2753d8042d673a812cafa7a6b70caa3</citedby><cites>FETCH-LOGICAL-c5019-7904e066517eade578c34535946749daae2753d8042d673a812cafa7a6b70caa3</cites><orcidid>0000-0002-9217-3202</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fglia.20189$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fglia.20189$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16965740$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15800928$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://univ-artois.hal.science/hal-00527237$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Boveri, Monica</creatorcontrib><creatorcontrib>Berezowski, Vincent</creatorcontrib><creatorcontrib>Price, Anna</creatorcontrib><creatorcontrib>Slupek, Stephanie</creatorcontrib><creatorcontrib>Lenfant, Anne-Marie</creatorcontrib><creatorcontrib>Benaud, Christelle</creatorcontrib><creatorcontrib>Hartung, Thomas</creatorcontrib><creatorcontrib>Cecchelli, Romeo</creatorcontrib><creatorcontrib>Prieto, Pilar</creatorcontrib><creatorcontrib>Dehouck, Marie-Pierre</creatorcontrib><title>Induction of blood-brain barrier properties in cultured brain capillary endothelial cells: Comparison between primary glial cells and C6 cell line</title><title>Glia</title><addtitle>Glia</addtitle><description>The communication between glial cells and brain capillary endothelial cells is crucial for a well‐differentiated blood‐brain barrier (BBB). It has been suggested that in vitro primary glial cells (GCs) be replaced by the glial C6 cell line to standardise the model further. This study compares directly the structural and functional differentiation of bovine brain capillary endothelial cells (BBCECs) induced by co‐culture with rat primary GCs or C6 cells, for the first time. Trans‐endothelial electrical resistance (TEER) measurements showed that under no condition were C6 cells able to reproduce TEER values as high as in the presence of GCs. At the same time, permeability of the BBCECs to both radioactive sucrose and FITC‐inulin was 2.5‐fold higher when cells were co‐cultured with C6 than with GCs. Furthermore, immunocytochemistry studies showed different cell morphology and less developed tight junction pattern of BBCECs co‐cultured with C6 toward GCs. Additionally, studies on P‐glycoprotein (P‐gp) showed much lower P‐gp presence and activity in BBCECs co‐cultured with C6 than GCs. Both VEGF mRNA expression and protein content were dramatically increased when compared with GCs, suggesting that VEGF could be one of the factors responsible for higher permeability of BBB. Our results clearly indicate that, in the presence of the glial C6 cell line, BBCECs did not differentiate as well as in the co‐culture with primary GCs at both structural and functional levels. © 2005 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biological and medical sciences</subject><subject>blood-brain barrier</subject><subject>Blood-Brain Barrier - cytology</subject><subject>Blood-Brain Barrier - metabolism</subject><subject>Brain - blood supply</subject><subject>Brain - physiology</subject><subject>Cattle</subject><subject>Cell Communication - physiology</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Line, Tumor</subject><subject>Cell Membrane Permeability - physiology</subject><subject>Cells, Cultured</subject><subject>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</subject><subject>Coculture Techniques</subject><subject>Electric Impedance</subject><subject>endothelial cells</subject><subject>Endothelial Cells - cytology</subject><subject>Endothelial Cells - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gap Junctions - metabolism</subject><subject>in vitro model</subject><subject>Inulin - pharmacokinetics</subject><subject>Isolated neuron and nerve. Neuroglia</subject><subject>Life Sciences</subject><subject>Membrane Potentials - physiology</subject><subject>Neuroglia - cytology</subject><subject>Neuroglia - metabolism</subject><subject>primary glial cells</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger - metabolism</subject><subject>Sucrose - pharmacokinetics</subject><subject>Up-Regulation - physiology</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0894-1491</issn><issn>1098-1136</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c1u1DAQAOAIgWgpXHgA5AtIIKWME_8k3JYVbJeuygUEN2vizLYGb7LYCaWvwRPjbZbuDU7WWN_8aCbLnnI45QDF60vv8LQAXtX3smMOdZVzXqr72TFUtci5qPlR9ijGbwA8BfphdsRlBVAX1XH2e9m1ox1c37F-zRrf923eBHQdazAER4FtQ7-lMDiKLP3a0Q9joJZNyOLWeY_hhlHX9sMVpVE8s-R9fMPm_WaLwcVUu6HhmqhLxdxmpy8PjmHXsrm6DZh3HT3OHqzRR3qyf0-yz-_ffZqf5auPi-V8tsqtBF7nugZBoJTkmrAlqStbClnKWigt6haRCi3LtgJRtEqXWPHC4ho1qkaDRSxPspdT3Sv0Zj-Y6dGZs9nK7P4AZKGLUv_kyb6YbNrGj5HiYDYu7ibGjvoxGlWBFKIs_gu5LgVXoBJ8NUEb-hgDre9G4GB2ZzW7HZnbsyb8bF91bDbUHuj-jgk83wOMFv06YGddPDhVK6kFJMcnd-083fyjpVmslrO_zfMpx8WBft3lYPhu0l61NF8uFqb6eq7O3158MIvyD-zZydQ</recordid><startdate>20050815</startdate><enddate>20050815</enddate><creator>Boveri, Monica</creator><creator>Berezowski, Vincent</creator><creator>Price, Anna</creator><creator>Slupek, Stephanie</creator><creator>Lenfant, Anne-Marie</creator><creator>Benaud, Christelle</creator><creator>Hartung, Thomas</creator><creator>Cecchelli, Romeo</creator><creator>Prieto, Pilar</creator><creator>Dehouck, Marie-Pierre</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-9217-3202</orcidid></search><sort><creationdate>20050815</creationdate><title>Induction of blood-brain barrier properties in cultured brain capillary endothelial cells: Comparison between primary glial cells and C6 cell line</title><author>Boveri, Monica ; Berezowski, Vincent ; Price, Anna ; Slupek, Stephanie ; Lenfant, Anne-Marie ; Benaud, Christelle ; Hartung, Thomas ; Cecchelli, Romeo ; Prieto, Pilar ; Dehouck, Marie-Pierre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5019-7904e066517eade578c34535946749daae2753d8042d673a812cafa7a6b70caa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism</topic><topic>Biochemistry, Molecular Biology</topic><topic>Biological and medical sciences</topic><topic>blood-brain barrier</topic><topic>Blood-Brain Barrier - cytology</topic><topic>Blood-Brain Barrier - metabolism</topic><topic>Brain - blood supply</topic><topic>Brain - physiology</topic><topic>Cattle</topic><topic>Cell Communication - physiology</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Line, Tumor</topic><topic>Cell Membrane Permeability - physiology</topic><topic>Cells, Cultured</topic><topic>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</topic><topic>Coculture Techniques</topic><topic>Electric Impedance</topic><topic>endothelial cells</topic><topic>Endothelial Cells - cytology</topic><topic>Endothelial Cells - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gap Junctions - metabolism</topic><topic>in vitro model</topic><topic>Inulin - pharmacokinetics</topic><topic>Isolated neuron and nerve. Neuroglia</topic><topic>Life Sciences</topic><topic>Membrane Potentials - physiology</topic><topic>Neuroglia - cytology</topic><topic>Neuroglia - metabolism</topic><topic>primary glial cells</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Messenger - metabolism</topic><topic>Sucrose - pharmacokinetics</topic><topic>Up-Regulation - physiology</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boveri, Monica</creatorcontrib><creatorcontrib>Berezowski, Vincent</creatorcontrib><creatorcontrib>Price, Anna</creatorcontrib><creatorcontrib>Slupek, Stephanie</creatorcontrib><creatorcontrib>Lenfant, Anne-Marie</creatorcontrib><creatorcontrib>Benaud, Christelle</creatorcontrib><creatorcontrib>Hartung, Thomas</creatorcontrib><creatorcontrib>Cecchelli, Romeo</creatorcontrib><creatorcontrib>Prieto, Pilar</creatorcontrib><creatorcontrib>Dehouck, Marie-Pierre</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Glia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boveri, Monica</au><au>Berezowski, Vincent</au><au>Price, Anna</au><au>Slupek, Stephanie</au><au>Lenfant, Anne-Marie</au><au>Benaud, Christelle</au><au>Hartung, Thomas</au><au>Cecchelli, Romeo</au><au>Prieto, Pilar</au><au>Dehouck, Marie-Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of blood-brain barrier properties in cultured brain capillary endothelial cells: Comparison between primary glial cells and C6 cell line</atitle><jtitle>Glia</jtitle><addtitle>Glia</addtitle><date>2005-08-15</date><risdate>2005</risdate><volume>51</volume><issue>3</issue><spage>187</spage><epage>198</epage><pages>187-198</pages><issn>0894-1491</issn><eissn>1098-1136</eissn><coden>GLIAEJ</coden><abstract>The communication between glial cells and brain capillary endothelial cells is crucial for a well‐differentiated blood‐brain barrier (BBB). It has been suggested that in vitro primary glial cells (GCs) be replaced by the glial C6 cell line to standardise the model further. This study compares directly the structural and functional differentiation of bovine brain capillary endothelial cells (BBCECs) induced by co‐culture with rat primary GCs or C6 cells, for the first time. Trans‐endothelial electrical resistance (TEER) measurements showed that under no condition were C6 cells able to reproduce TEER values as high as in the presence of GCs. At the same time, permeability of the BBCECs to both radioactive sucrose and FITC‐inulin was 2.5‐fold higher when cells were co‐cultured with C6 than with GCs. Furthermore, immunocytochemistry studies showed different cell morphology and less developed tight junction pattern of BBCECs co‐cultured with C6 toward GCs. Additionally, studies on P‐glycoprotein (P‐gp) showed much lower P‐gp presence and activity in BBCECs co‐cultured with C6 than GCs. Both VEGF mRNA expression and protein content were dramatically increased when compared with GCs, suggesting that VEGF could be one of the factors responsible for higher permeability of BBB. Our results clearly indicate that, in the presence of the glial C6 cell line, BBCECs did not differentiate as well as in the co‐culture with primary GCs at both structural and functional levels. © 2005 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>15800928</pmid><doi>10.1002/glia.20189</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-9217-3202</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0894-1491 |
| ispartof | Glia, 2005-08, Vol.51 (3), p.187-198 |
| issn | 0894-1491 1098-1136 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_00527237v1 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Animals, Newborn ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism Biochemistry, Molecular Biology Biological and medical sciences blood-brain barrier Blood-Brain Barrier - cytology Blood-Brain Barrier - metabolism Brain - blood supply Brain - physiology Cattle Cell Communication - physiology Cell Differentiation - physiology Cell Line, Tumor Cell Membrane Permeability - physiology Cells, Cultured Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges Coculture Techniques Electric Impedance endothelial cells Endothelial Cells - cytology Endothelial Cells - metabolism Fundamental and applied biological sciences. Psychology Gap Junctions - metabolism in vitro model Inulin - pharmacokinetics Isolated neuron and nerve. Neuroglia Life Sciences Membrane Potentials - physiology Neuroglia - cytology Neuroglia - metabolism primary glial cells Rats Rats, Sprague-Dawley RNA, Messenger - metabolism Sucrose - pharmacokinetics Up-Regulation - physiology Vascular Endothelial Growth Factor A - genetics Vascular Endothelial Growth Factor A - metabolism Vertebrates: nervous system and sense organs |
| title | Induction of blood-brain barrier properties in cultured brain capillary endothelial cells: Comparison between primary glial cells and C6 cell line |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T16%3A34%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Induction%20of%20blood-brain%20barrier%20properties%20in%20cultured%20brain%20capillary%20endothelial%20cells:%20Comparison%20between%20primary%20glial%20cells%20and%20C6%20cell%20line&rft.jtitle=Glia&rft.au=Boveri,%20Monica&rft.date=2005-08-15&rft.volume=51&rft.issue=3&rft.spage=187&rft.epage=198&rft.pages=187-198&rft.issn=0894-1491&rft.eissn=1098-1136&rft.coden=GLIAEJ&rft_id=info:doi/10.1002/glia.20189&rft_dat=%3Cproquest_hal_p%3E68054432%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17341606&rft_id=info:pmid/15800928&rfr_iscdi=true |