Culture enrichment assists the diagnosis of cattle botulism by a monoclonal antibody based sandwich ELISA

Monoclonal antibodies (MAbs) obtained from a mouse immunised with Clostridium botulinum type D toxoid were developed into a sandwich ELISA (sELISA) format that was able to detect type D toxin and types C and D toxin complexes. The sELISA was examined for its potential to replace the mouse bioassay a...

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Veröffentlicht in:	Veterinary microbiology 2010-07, Vol.144 (1), p.226-230
Hauptverfasser:	Brooks, C.E., Clarke, H.J., Finlay, D.A., McConnell, W., Graham, D.A., Ball, H.J.
Format:	Artikel
Sprache:	eng
Schlagworte:	animal use refinement Animals Antibodies, Monoclonal - immunology Bacterial diseases Bacterial diseases of the nervous system. Bacterial myositis bacterial toxins bioassays Biological and medical sciences Biological Assay - methods botulism Botulism - diagnosis Botulism - immunology Botulism - veterinary C. botulinum Cattle Cattle botulism cattle diseases Cattle Diseases - diagnosis Cattle Diseases - immunology Clostridium botulinum Clostridium botulinum - immunology disease detection disease diagnosis ELISA enrichment culture enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Fundamental and applied biological sciences. Psychology heat treatment Human bacterial diseases Immunoblotting - methods in vitro culture Infectious diseases intestines Intestines - microbiology Ireland Lymphocytes - immunology Medical sciences mice Mice - immunology Mice, Inbred BALB C - immunology Microbiology Monoclonal antibodies neurotoxins new methods optimization pathotypes Reproducibility of Results screening Sensitivity and Specificity Spleen - immunology Spores, Bacterial - immunology Spores, Bacterial - isolation & purification validity
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