High-level expression of nonglycosylated human pancreatic lipase-related protein 2 in Pichia pastoris

The human pancreatic lipase-related protein 2 (HPLRP2) was produced in the methylotrophic yeast Pichia pastoris. The HPLRP2 cDNA corresponding to the protein coding sequence including the native signal sequence, was cloned into the pPIC9K vector and integrated into the genome of P. pastoris. P. past...

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Veröffentlicht in:	Protein expression and purification 2006-10, Vol.49 (2), p.284-291
Hauptverfasser:	Sebban-Kreuzer, Corinne, Deprez-Beauclair, Paule, Berton, Amelie, Crenon, Isabelle
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Bacterial Agents Anti-Bacterial Agents - pharmacology Biochemistry, Molecular Biology Cloning, Molecular Cloning, Molecular - methods Expression Gene Expression Glycosylation Glycosylation - drug effects HPLRP2 Humans Life Sciences Lipase Lipase - biosynthesis Lipase - genetics Lipase - secretion Pichia Pichia - genetics Pichia - metabolism Pichia pastoris Protein Processing, Post-Translational Protein Processing, Post-Translational - drug effects Protein Processing, Post-Translational - genetics Purification Recombinant Proteins Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - secretion Tunicamycin Tunicamycin - pharmacology
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