Glycosylated Quantum Dots for the Selective Labelling of Kluyveromyces bulgaricus and Saccharomyces cerevisiae Yeast Strains
Highly fluorescent CdTe quantum dots (QDs) stabilized by thioglycolic acid (TGA) were prepared by an aqueous solution approach and used as fluorescent labels in detecting yeast cells. Sugars (mannose, galactose or glucose) were adsorbed on CdTe@TGA QDs and the interaction of these nanoparticles with...
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| Veröffentlicht in: | Journal of fluorescence 2010-03, Vol.20 (2), p.591-597 |
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| container_title | Journal of fluorescence |
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| creator | Coulon, Joël Thouvenin, Ilan Aldeek, Fadi Balan, Lavinia Schneider, Raphaël |
| description | Highly fluorescent CdTe quantum dots (QDs) stabilized by thioglycolic acid (TGA) were prepared by an aqueous solution approach and used as fluorescent labels in detecting yeast cells. Sugars (mannose, galactose or glucose) were adsorbed on CdTe@TGA QDs and the interaction of these nanoparticles with yeast cells was studied by fluorescence microscopy. Results obtained demonstrate that galactose and mannose functionalized QDs associate respectively with
Kluyveromyces bulgaricus
and
Saccharomyces cerevisiae
yeast strains due to saccharide/lectin specific recognition. Glucose-functionalized CdTe QDs, which are not recognized by cell lectins, preferentially localize in the bud scars of
S. cerevisiae. |
| doi_str_mv | 10.1007/s10895-009-0590-8 |
| format | Article |
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Kluyveromyces bulgaricus
and
Saccharomyces cerevisiae
yeast strains due to saccharide/lectin specific recognition. Glucose-functionalized CdTe QDs, which are not recognized by cell lectins, preferentially localize in the bud scars of
S. cerevisiae.</description><identifier>ISSN: 1053-0509</identifier><identifier>EISSN: 1573-4994</identifier><identifier>DOI: 10.1007/s10895-009-0590-8</identifier><identifier>PMID: 20058182</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Analytical Chemistry ; Biochemistry ; Biological and Medical Physics ; Biomedical and Life Sciences ; Biomedicine ; Biophysics ; Biotechnology ; Cadmium Compounds - chemistry ; Cadmium tellurides ; Chemical Sciences ; Fluorescence ; Galactose - chemistry ; Glucose - chemistry ; Glycosylation ; Kluyveromyces ; Kluyveromyces - chemistry ; Kluyveromyces - isolation & purification ; Lectins ; Lectins - chemistry ; Mannose ; Mannose - chemistry ; Material chemistry ; Microscopy, Atomic Force ; Microscopy, Electron, Transmission ; Microscopy, Fluorescence ; Mycology - methods ; Original Paper ; Polymers ; Quantum Dots ; Recognition ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - chemistry ; Saccharomyces cerevisiae - isolation & purification ; Strain ; Tellurium - chemistry ; Water - chemistry ; X-Ray Diffraction ; Yeast</subject><ispartof>Journal of fluorescence, 2010-03, Vol.20 (2), p.591-597</ispartof><rights>Springer Science+Business Media, LLC 2009</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-a084af95ea2241998e38d169fbf6745387211ac3b1efcb3719de7bf6db6350e23</citedby><cites>FETCH-LOGICAL-c442t-a084af95ea2241998e38d169fbf6745387211ac3b1efcb3719de7bf6db6350e23</cites><orcidid>0000-0002-6870-6902 ; 0000-0003-0144-401X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10895-009-0590-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10895-009-0590-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,777,781,882,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20058182$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00473789$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Coulon, Joël</creatorcontrib><creatorcontrib>Thouvenin, Ilan</creatorcontrib><creatorcontrib>Aldeek, Fadi</creatorcontrib><creatorcontrib>Balan, Lavinia</creatorcontrib><creatorcontrib>Schneider, Raphaël</creatorcontrib><title>Glycosylated Quantum Dots for the Selective Labelling of Kluyveromyces bulgaricus and Saccharomyces cerevisiae Yeast Strains</title><title>Journal of fluorescence</title><addtitle>J Fluoresc</addtitle><addtitle>J Fluoresc</addtitle><description>Highly fluorescent CdTe quantum dots (QDs) stabilized by thioglycolic acid (TGA) were prepared by an aqueous solution approach and used as fluorescent labels in detecting yeast cells. Sugars (mannose, galactose or glucose) were adsorbed on CdTe@TGA QDs and the interaction of these nanoparticles with yeast cells was studied by fluorescence microscopy. Results obtained demonstrate that galactose and mannose functionalized QDs associate respectively with
Kluyveromyces bulgaricus
and
Saccharomyces cerevisiae
yeast strains due to saccharide/lectin specific recognition. Glucose-functionalized CdTe QDs, which are not recognized by cell lectins, preferentially localize in the bud scars of
S. cerevisiae.</description><subject>Analytical Chemistry</subject><subject>Biochemistry</subject><subject>Biological and Medical Physics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Biophysics</subject><subject>Biotechnology</subject><subject>Cadmium Compounds - chemistry</subject><subject>Cadmium tellurides</subject><subject>Chemical Sciences</subject><subject>Fluorescence</subject><subject>Galactose - chemistry</subject><subject>Glucose - chemistry</subject><subject>Glycosylation</subject><subject>Kluyveromyces</subject><subject>Kluyveromyces - chemistry</subject><subject>Kluyveromyces - isolation & purification</subject><subject>Lectins</subject><subject>Lectins - chemistry</subject><subject>Mannose</subject><subject>Mannose - chemistry</subject><subject>Material chemistry</subject><subject>Microscopy, Atomic Force</subject><subject>Microscopy, Electron, Transmission</subject><subject>Microscopy, Fluorescence</subject><subject>Mycology - methods</subject><subject>Original Paper</subject><subject>Polymers</subject><subject>Quantum Dots</subject><subject>Recognition</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - chemistry</subject><subject>Saccharomyces cerevisiae - isolation & purification</subject><subject>Strain</subject><subject>Tellurium - chemistry</subject><subject>Water - chemistry</subject><subject>X-Ray Diffraction</subject><subject>Yeast</subject><issn>1053-0509</issn><issn>1573-4994</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9v1DAQxSMEon_gA3BBvgGHwDi21_axaqGtWAmhhQMna-JMdlNl42InK0Xiw-NV2h578sjvN08z84riHYfPHEB_SRyMVSWALUFZKM2L4pQrLUpprXyZa1AiK2BPirOU7iCDRprXxUkFoAw31Wnx77qffUhzjyM17OeEwzjt2VUYE2tDZOOO2IZ68mN3ILbGmvq-G7YstOx7P80HimE_e0qsnvotxs5PieHQsA16v8NH0VOkQ5c6JPaHMI1sM0bshvSmeNVin-jtw3te_P729dflTbn-cX17ebEuvZTVWCIYia1VhFUlubWGhGn4yrZ1u9JSCaMrztGLmlPra6G5bUhnralXQgFV4rz4tPjusHf3sdtjnF3Azt1crN3xD0BqoY098Mx-WNj7GP5OlEa375LPa-NAYUrOVishADhk8uOzJNdKKGm4PpryBfUxpBSpfZqCgztG6ZYo8yDWHaN0Jve8f7Cf6j01Tx2P2WWgWoCUpWFL0d2FKQ75kM-4_gefpqpL</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>Coulon, Joël</creator><creator>Thouvenin, Ilan</creator><creator>Aldeek, Fadi</creator><creator>Balan, Lavinia</creator><creator>Schneider, Raphaël</creator><general>Springer US</general><general>Springer Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>M7N</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-6870-6902</orcidid><orcidid>https://orcid.org/0000-0003-0144-401X</orcidid></search><sort><creationdate>20100301</creationdate><title>Glycosylated Quantum Dots for the Selective Labelling of Kluyveromyces bulgaricus and Saccharomyces cerevisiae Yeast Strains</title><author>Coulon, Joël ; Thouvenin, Ilan ; Aldeek, Fadi ; Balan, Lavinia ; Schneider, Raphaël</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-a084af95ea2241998e38d169fbf6745387211ac3b1efcb3719de7bf6db6350e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Analytical Chemistry</topic><topic>Biochemistry</topic><topic>Biological and Medical Physics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Biophysics</topic><topic>Biotechnology</topic><topic>Cadmium Compounds - chemistry</topic><topic>Cadmium tellurides</topic><topic>Chemical Sciences</topic><topic>Fluorescence</topic><topic>Galactose - chemistry</topic><topic>Glucose - chemistry</topic><topic>Glycosylation</topic><topic>Kluyveromyces</topic><topic>Kluyveromyces - chemistry</topic><topic>Kluyveromyces - isolation & purification</topic><topic>Lectins</topic><topic>Lectins - chemistry</topic><topic>Mannose</topic><topic>Mannose - chemistry</topic><topic>Material chemistry</topic><topic>Microscopy, Atomic Force</topic><topic>Microscopy, Electron, Transmission</topic><topic>Microscopy, Fluorescence</topic><topic>Mycology - methods</topic><topic>Original Paper</topic><topic>Polymers</topic><topic>Quantum Dots</topic><topic>Recognition</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - chemistry</topic><topic>Saccharomyces cerevisiae - isolation & purification</topic><topic>Strain</topic><topic>Tellurium - chemistry</topic><topic>Water - chemistry</topic><topic>X-Ray Diffraction</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coulon, Joël</creatorcontrib><creatorcontrib>Thouvenin, Ilan</creatorcontrib><creatorcontrib>Aldeek, Fadi</creatorcontrib><creatorcontrib>Balan, Lavinia</creatorcontrib><creatorcontrib>Schneider, Raphaël</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of fluorescence</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coulon, Joël</au><au>Thouvenin, Ilan</au><au>Aldeek, Fadi</au><au>Balan, Lavinia</au><au>Schneider, Raphaël</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glycosylated Quantum Dots for the Selective Labelling of Kluyveromyces bulgaricus and Saccharomyces cerevisiae Yeast Strains</atitle><jtitle>Journal of fluorescence</jtitle><stitle>J Fluoresc</stitle><addtitle>J Fluoresc</addtitle><date>2010-03-01</date><risdate>2010</risdate><volume>20</volume><issue>2</issue><spage>591</spage><epage>597</epage><pages>591-597</pages><issn>1053-0509</issn><eissn>1573-4994</eissn><abstract>Highly fluorescent CdTe quantum dots (QDs) stabilized by thioglycolic acid (TGA) were prepared by an aqueous solution approach and used as fluorescent labels in detecting yeast cells. Sugars (mannose, galactose or glucose) were adsorbed on CdTe@TGA QDs and the interaction of these nanoparticles with yeast cells was studied by fluorescence microscopy. Results obtained demonstrate that galactose and mannose functionalized QDs associate respectively with
Kluyveromyces bulgaricus
and
Saccharomyces cerevisiae
yeast strains due to saccharide/lectin specific recognition. Glucose-functionalized CdTe QDs, which are not recognized by cell lectins, preferentially localize in the bud scars of
S. cerevisiae.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>20058182</pmid><doi>10.1007/s10895-009-0590-8</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-6870-6902</orcidid><orcidid>https://orcid.org/0000-0003-0144-401X</orcidid></addata></record> |
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| subjects | Analytical Chemistry Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biomedicine Biophysics Biotechnology Cadmium Compounds - chemistry Cadmium tellurides Chemical Sciences Fluorescence Galactose - chemistry Glucose - chemistry Glycosylation Kluyveromyces Kluyveromyces - chemistry Kluyveromyces - isolation & purification Lectins Lectins - chemistry Mannose Mannose - chemistry Material chemistry Microscopy, Atomic Force Microscopy, Electron, Transmission Microscopy, Fluorescence Mycology - methods Original Paper Polymers Quantum Dots Recognition Saccharomyces cerevisiae Saccharomyces cerevisiae - chemistry Saccharomyces cerevisiae - isolation & purification Strain Tellurium - chemistry Water - chemistry X-Ray Diffraction Yeast |
| title | Glycosylated Quantum Dots for the Selective Labelling of Kluyveromyces bulgaricus and Saccharomyces cerevisiae Yeast Strains |
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