Cold plasma functionalized TeraHertz BioMEMS for enzyme reaction analysis
In this paper, we describe the development, functionalization and functionality testing of a TeraHertz (THz) Bio-MicroElectroMechanical System (BioMEMS) dedicated to enzyme reaction analysis. The microdevice was fabricated by mixing clean room microfabrication with cold plasma deposition. The first...
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description | In this paper, we describe the development, functionalization and functionality testing of a TeraHertz (THz) Bio-MicroElectroMechanical System (BioMEMS) dedicated to enzyme reaction analysis. The microdevice was fabricated by mixing clean room microfabrication with cold plasma deposition. The first is used to build the microfluidic circuits and the THz sensor, while the later serves for the polymerization of allylamine using a homemade glow discharge plasma reactor for a subsequent immobilization of enzymatic biocatalysts. Thermal stability of the deposited plasma polymer has been investigated by infrared spectroscopy. Fluorescent detection confirmed the efficiency of the immobilization and the enzyme hydrolysis into the BioMEMS microchannels. For the first time, the progression of the hydrolysis reaction over time was monitored by the THz sensor connected to a vectorial network analyzer. Preliminary results showed that sub-THz transmission measurements are able to discriminate different solid films, various aqueous media and exhibit specific transmission behavior for the enzyme hydrolysis reaction in the spectral range 0.06–0.11
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doi_str_mv | 10.1016/j.bios.2009.06.029 |
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THz.</description><subject>Allylamine - chemistry</subject><subject>Animals</subject><subject>Biocatalysis</subject><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>BioMEMS</subject><subject>Biotechnology</subject><subject>Cattle</subject><subject>Cold plasma</subject><subject>Engineering Sciences</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Enzymes, Immobilized - metabolism</subject><subject>Equipment Design</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrolysis</subject><subject>Methods. Procedures. Technologies</subject><subject>Micro-Electrical-Mechanical Systems - instrumentation</subject><subject>Micro-Electrical-Mechanical Systems - methods</subject><subject>Microtechnology - methods</subject><subject>Sub-TeraHertz spectroscopy</subject><subject>Thin films</subject><subject>Trypsin - chemistry</subject><subject>Trypsin - metabolism</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c9r2zAUwHExNtas2z-ww_BlGzvYfZKsH4ZeutA1hZQe1p2FbD8xBdvKJKeQ_PWzl9DeupPg8XkPwZeQjxQKClRebIrah1QwgKoAWQCrXpEF1YrnJePiNVlAJWQupORn5F1KGwBQtIK35IxWEnQJbEFul6Frs21nU28ztxua0YfBdv6AbfaA0a4wjofsuw9313c_MxdihsNh32MW0f6zmZ34Pvn0nrxxtkv44fSek18_rh-Wq3x9f3O7vFrnjSjVmEspAFtBObq6dQ4QVa1r6rCmVFU1lxq5LGut0SldS6cVTmOmFdhWthL4Ofl2vPvbdmYbfW_j3gTrzepqbeYZQKmopuUjnezXo93G8GeHaTS9Tw12nR0w7JJRnAulpJjllxclLxWTVcn_CxkoxbiuJsiOsIkhpYju6bMUzNzPbMzcz8z9DEgz9ZuWPp2u7-oe2-eVU7AJfD4BmxrbuWiHxqcnx2ilSiFmd3l0OKV49BhNajwODbY-YjOaNviX_vEX1wm3rQ</recordid><startdate>20090915</startdate><enddate>20090915</enddate><creator>Abbas, Abdennour</creator><creator>Treizebre, Anthony</creator><creator>Supiot, Philippe</creator><creator>Bourzgui, Nour-Eddine</creator><creator>Guillochon, Didier</creator><creator>Vercaigne-Marko, Dominique</creator><creator>Bocquet, Bertrand</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SP</scope><scope>7U5</scope><scope>L7M</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-2265-8095</orcidid><orcidid>https://orcid.org/0000-0003-1018-2912</orcidid><orcidid>https://orcid.org/0000-0003-0352-6422</orcidid></search><sort><creationdate>20090915</creationdate><title>Cold plasma functionalized TeraHertz BioMEMS for enzyme reaction analysis</title><author>Abbas, Abdennour ; Treizebre, Anthony ; Supiot, Philippe ; Bourzgui, Nour-Eddine ; Guillochon, Didier ; Vercaigne-Marko, Dominique ; Bocquet, Bertrand</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c547t-6650ed513efbdff0ee7b8b1feb1179b368e364b88ef78b6f87e79b2870ad6d603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Allylamine - chemistry</topic><topic>Animals</topic><topic>Biocatalysis</topic><topic>Bioconversions. Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>BioMEMS</topic><topic>Biotechnology</topic><topic>Cattle</topic><topic>Cold plasma</topic><topic>Engineering Sciences</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Enzymes, Immobilized - metabolism</topic><topic>Equipment Design</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrolysis</topic><topic>Methods. Procedures. Technologies</topic><topic>Micro-Electrical-Mechanical Systems - instrumentation</topic><topic>Micro-Electrical-Mechanical Systems - methods</topic><topic>Microtechnology - methods</topic><topic>Sub-TeraHertz spectroscopy</topic><topic>Thin films</topic><topic>Trypsin - chemistry</topic><topic>Trypsin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abbas, Abdennour</creatorcontrib><creatorcontrib>Treizebre, Anthony</creatorcontrib><creatorcontrib>Supiot, Philippe</creatorcontrib><creatorcontrib>Bourzgui, Nour-Eddine</creatorcontrib><creatorcontrib>Guillochon, Didier</creatorcontrib><creatorcontrib>Vercaigne-Marko, Dominique</creatorcontrib><creatorcontrib>Bocquet, Bertrand</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abbas, Abdennour</au><au>Treizebre, Anthony</au><au>Supiot, Philippe</au><au>Bourzgui, Nour-Eddine</au><au>Guillochon, Didier</au><au>Vercaigne-Marko, Dominique</au><au>Bocquet, Bertrand</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cold plasma functionalized TeraHertz BioMEMS for enzyme reaction analysis</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2009-09-15</date><risdate>2009</risdate><volume>25</volume><issue>1</issue><spage>154</spage><epage>160</epage><pages>154-160</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>In this paper, we describe the development, functionalization and functionality testing of a TeraHertz (THz) Bio-MicroElectroMechanical System (BioMEMS) dedicated to enzyme reaction analysis. The microdevice was fabricated by mixing clean room microfabrication with cold plasma deposition. The first is used to build the microfluidic circuits and the THz sensor, while the later serves for the polymerization of allylamine using a homemade glow discharge plasma reactor for a subsequent immobilization of enzymatic biocatalysts. Thermal stability of the deposited plasma polymer has been investigated by infrared spectroscopy. Fluorescent detection confirmed the efficiency of the immobilization and the enzyme hydrolysis into the BioMEMS microchannels. For the first time, the progression of the hydrolysis reaction over time was monitored by the THz sensor connected to a vectorial network analyzer. Preliminary results showed that sub-THz transmission measurements are able to discriminate different solid films, various aqueous media and exhibit specific transmission behavior for the enzyme hydrolysis reaction in the spectral range 0.06–0.11
THz.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>19608402</pmid><doi>10.1016/j.bios.2009.06.029</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-2265-8095</orcidid><orcidid>https://orcid.org/0000-0003-1018-2912</orcidid><orcidid>https://orcid.org/0000-0003-0352-6422</orcidid></addata></record> |
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subjects | Allylamine - chemistry Animals Biocatalysis Bioconversions. Hemisynthesis Biological and medical sciences BioMEMS Biotechnology Cattle Cold plasma Engineering Sciences Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Equipment Design Fundamental and applied biological sciences. Psychology Hydrolysis Methods. Procedures. Technologies Micro-Electrical-Mechanical Systems - instrumentation Micro-Electrical-Mechanical Systems - methods Microtechnology - methods Sub-TeraHertz spectroscopy Thin films Trypsin - chemistry Trypsin - metabolism |
title | Cold plasma functionalized TeraHertz BioMEMS for enzyme reaction analysis |
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