Rational design and immunogenicity of liposome-based diepitope constructs: Application to synthetic oligosaccharides mimicking the Shigella flexneri 2a O-antigen
Abstract We have designed chemically defined diepitope constructs consisting of liposomes displaying at their surface synthetic oligosaccharides mimicking the O-antigen of the Shigella flexneri 2a lipopolysaccharide (B-cell epitope) and influenza hemagglutinin peptide HA 307–319 (Th epitope). Using...
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| Veröffentlicht in: | Vaccine 2009-08, Vol.27 (39), p.5419-5426 |
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| creator | Said Hassane, Fatouma Phalipon, Armelle Tanguy, Myriam Guerreiro, Catherine Bélot, Frédéric Frisch, Benoît Mulard, Laurence A Schuber, Francis |
| description | Abstract We have designed chemically defined diepitope constructs consisting of liposomes displaying at their surface synthetic oligosaccharides mimicking the O-antigen of the Shigella flexneri 2a lipopolysaccharide (B-cell epitope) and influenza hemagglutinin peptide HA 307–319 (Th epitope). Using well controlled and high-yielding covalent bioconjugation reactions, the two structurally independent epitopes were coupled to the lipopeptide Pam3 CAG, i.e. a TLR2 ligand known for its adjuvant properties, anchored in preformed vesicles. The synthetic construct containing a pentadecasaccharide corresponding to three O-antigen repeating units triggered T-dependent anti-oligosaccharide and anti- S. flexneri 2a LPS antibody responses when administered i.m. to BALB/c mice. Moreover, the long-lasting anti-LPS antibody response afforded protection against a S. flexneri 2a challenge. These results show that liposome diepitope constructs could be attractive alternatives in the development of synthetic carbohydrate-based vaccines. |
| doi_str_mv | 10.1016/j.vaccine.2009.06.031 |
| format | Article |
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Using well controlled and high-yielding covalent bioconjugation reactions, the two structurally independent epitopes were coupled to the lipopeptide Pam3 CAG, i.e. a TLR2 ligand known for its adjuvant properties, anchored in preformed vesicles. The synthetic construct containing a pentadecasaccharide corresponding to three O-antigen repeating units triggered T-dependent anti-oligosaccharide and anti- S. flexneri 2a LPS antibody responses when administered i.m. to BALB/c mice. Moreover, the long-lasting anti-LPS antibody response afforded protection against a S. flexneri 2a challenge. These results show that liposome diepitope constructs could be attractive alternatives in the development of synthetic carbohydrate-based vaccines.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>EISSN: 0264-410X</identifier><identifier>DOI: 10.1016/j.vaccine.2009.06.031</identifier><identifier>PMID: 19559116</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Adjuvants ; Allergy and Immunology ; Animals ; Antibodies, Bacterial - blood ; Antibodies, Bacterial - immunology ; Applied microbiology ; Bacterial Vaccines - immunology ; Bacteriology ; Biological and medical sciences ; Dysentery, Bacillary - immunology ; Dysentery, Bacillary - prevention & control ; Epitopes, B-Lymphocyte - immunology ; Epitopes, T-Lymphocyte - immunology ; Female ; Fundamental and applied biological sciences. Psychology ; Hemagglutinins - immunology ; Immunology ; Life Sciences ; Lipopeptides - chemistry ; Lipopeptides - immunology ; Liposomes ; Liposomes - immunology ; Mice ; Mice, Inbred BALB C ; Microbiology ; Miscellaneous ; Molecular Structure ; O Antigens - chemistry ; O Antigens - immunology ; Oligosaccharides - chemistry ; Oligosaccharides - immunology ; Orthomyxoviridae ; Shigella flexneri ; Shigella flexneri - immunology ; Shigella flexneri 2a O-antigen ; Synthetic oligosaccharide-based vaccination construct ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccines, Synthetic - immunology</subject><ispartof>Vaccine, 2009-08, Vol.27 (39), p.5419-5426</ispartof><rights>Elsevier Ltd</rights><rights>2009 Elsevier Ltd</rights><rights>2009 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c514t-cd94ee9de5d2f8cb26fa4d3e2859e02b03fb3d8498ce5bd3e2c250d2e5e58fc03</citedby><cites>FETCH-LOGICAL-c514t-cd94ee9de5d2f8cb26fa4d3e2859e02b03fb3d8498ce5bd3e2c250d2e5e58fc03</cites><orcidid>0000-0002-5622-1422 ; 0000-0002-8378-2508</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vaccine.2009.06.031$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3548,27923,27924,45994,64386</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21879259$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19559116$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00414241$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Said Hassane, Fatouma</creatorcontrib><creatorcontrib>Phalipon, Armelle</creatorcontrib><creatorcontrib>Tanguy, Myriam</creatorcontrib><creatorcontrib>Guerreiro, Catherine</creatorcontrib><creatorcontrib>Bélot, Frédéric</creatorcontrib><creatorcontrib>Frisch, Benoît</creatorcontrib><creatorcontrib>Mulard, Laurence A</creatorcontrib><creatorcontrib>Schuber, Francis</creatorcontrib><title>Rational design and immunogenicity of liposome-based diepitope constructs: Application to synthetic oligosaccharides mimicking the Shigella flexneri 2a O-antigen</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract We have designed chemically defined diepitope constructs consisting of liposomes displaying at their surface synthetic oligosaccharides mimicking the O-antigen of the Shigella flexneri 2a lipopolysaccharide (B-cell epitope) and influenza hemagglutinin peptide HA 307–319 (Th epitope). Using well controlled and high-yielding covalent bioconjugation reactions, the two structurally independent epitopes were coupled to the lipopeptide Pam3 CAG, i.e. a TLR2 ligand known for its adjuvant properties, anchored in preformed vesicles. The synthetic construct containing a pentadecasaccharide corresponding to three O-antigen repeating units triggered T-dependent anti-oligosaccharide and anti- S. flexneri 2a LPS antibody responses when administered i.m. to BALB/c mice. Moreover, the long-lasting anti-LPS antibody response afforded protection against a S. flexneri 2a challenge. These results show that liposome diepitope constructs could be attractive alternatives in the development of synthetic carbohydrate-based vaccines.</description><subject>Adjuvants</subject><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antibodies, Bacterial - immunology</subject><subject>Applied microbiology</subject><subject>Bacterial Vaccines - immunology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Dysentery, Bacillary - immunology</subject><subject>Dysentery, Bacillary - prevention & control</subject><subject>Epitopes, B-Lymphocyte - immunology</subject><subject>Epitopes, T-Lymphocyte - immunology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemagglutinins - immunology</subject><subject>Immunology</subject><subject>Life Sciences</subject><subject>Lipopeptides - chemistry</subject><subject>Lipopeptides - immunology</subject><subject>Liposomes</subject><subject>Liposomes - immunology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Structure</subject><subject>O Antigens - chemistry</subject><subject>O Antigens - immunology</subject><subject>Oligosaccharides - chemistry</subject><subject>Oligosaccharides - immunology</subject><subject>Orthomyxoviridae</subject><subject>Shigella flexneri</subject><subject>Shigella flexneri - immunology</subject><subject>Shigella flexneri 2a O-antigen</subject><subject>Synthetic oligosaccharide-based vaccination construct</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vaccines, Synthetic - immunology</subject><issn>0264-410X</issn><issn>1873-2518</issn><issn>0264-410X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFksGO0zAQhiMEYpeFRwD5AohDiu3EacIBVK2ARaq0EgsSN8uxJ-10EzvYTkUfhzfFodUicYCTpfE3M7_9_1n2lNEFo6x6vVvsldZoYcEpbRa0WtCC3cvOWb0sci5YfT87p7wq85LRb2fZoxB2lFJRsOZhdsYaIRrGqvPs52cV0VnVEwMBN5YoawgOw2TdBixqjAfiOtLj6IIbIG9VAEMMwojRjUC0syH6ScfwhqzGsUf9ex6JjoSDjVuIqInrceNCkrtVHtMeMuCA-hbthiSC3GxxA32vSNfDDwseCVfkOlc2prp9nD3oVB_gyem8yL5-eP_l8ipfX3_8dLla51qwMubaNCVAY0AY3tW65VWnSlMAr0UDlLe06NrC1GVTaxDtfKG5oIaDAFF3mhYX2avj3K3q5ehxUP4gnUJ5tVrLuUZpyUpesj1L7MsjO3r3fYIQ5YBBz2-w4KYgl2VFl8tlwRP54p9ktRS1SGQCxRHU3oXgobvTwKicHZc7eXJczo5LWsnkeOp7dlowtQOYP10nixPw_ASooFXfeWU1hjuOp8A0XDSJe3fkIH3yHsHLoBGsBoMedJTG4X-lvP1rgu4xZUj1t3CAsHOTT0ELksnAJZU3czzndNKG0rrmvPgFNbflIA</recordid><startdate>20090827</startdate><enddate>20090827</enddate><creator>Said Hassane, Fatouma</creator><creator>Phalipon, Armelle</creator><creator>Tanguy, Myriam</creator><creator>Guerreiro, Catherine</creator><creator>Bélot, Frédéric</creator><creator>Frisch, Benoît</creator><creator>Mulard, Laurence A</creator><creator>Schuber, Francis</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-5622-1422</orcidid><orcidid>https://orcid.org/0000-0002-8378-2508</orcidid></search><sort><creationdate>20090827</creationdate><title>Rational design and immunogenicity of liposome-based diepitope constructs: Application to synthetic oligosaccharides mimicking the Shigella flexneri 2a O-antigen</title><author>Said Hassane, Fatouma ; Phalipon, Armelle ; Tanguy, Myriam ; Guerreiro, Catherine ; Bélot, Frédéric ; Frisch, Benoît ; Mulard, Laurence A ; Schuber, Francis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c514t-cd94ee9de5d2f8cb26fa4d3e2859e02b03fb3d8498ce5bd3e2c250d2e5e58fc03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adjuvants</topic><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>Antibodies, Bacterial - immunology</topic><topic>Applied microbiology</topic><topic>Bacterial Vaccines - immunology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Dysentery, Bacillary - immunology</topic><topic>Dysentery, Bacillary - prevention & control</topic><topic>Epitopes, B-Lymphocyte - immunology</topic><topic>Epitopes, T-Lymphocyte - immunology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemagglutinins - immunology</topic><topic>Immunology</topic><topic>Life Sciences</topic><topic>Lipopeptides - chemistry</topic><topic>Lipopeptides - immunology</topic><topic>Liposomes</topic><topic>Liposomes - immunology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Structure</topic><topic>O Antigens - chemistry</topic><topic>O Antigens - immunology</topic><topic>Oligosaccharides - chemistry</topic><topic>Oligosaccharides - immunology</topic><topic>Orthomyxoviridae</topic><topic>Shigella flexneri</topic><topic>Shigella flexneri - immunology</topic><topic>Shigella flexneri 2a O-antigen</topic><topic>Synthetic oligosaccharide-based vaccination construct</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Vaccines, Synthetic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Said Hassane, Fatouma</creatorcontrib><creatorcontrib>Phalipon, Armelle</creatorcontrib><creatorcontrib>Tanguy, Myriam</creatorcontrib><creatorcontrib>Guerreiro, Catherine</creatorcontrib><creatorcontrib>Bélot, Frédéric</creatorcontrib><creatorcontrib>Frisch, Benoît</creatorcontrib><creatorcontrib>Mulard, Laurence A</creatorcontrib><creatorcontrib>Schuber, Francis</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Said Hassane, Fatouma</au><au>Phalipon, Armelle</au><au>Tanguy, Myriam</au><au>Guerreiro, Catherine</au><au>Bélot, Frédéric</au><au>Frisch, Benoît</au><au>Mulard, Laurence A</au><au>Schuber, Francis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rational design and immunogenicity of liposome-based diepitope constructs: Application to synthetic oligosaccharides mimicking the Shigella flexneri 2a O-antigen</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2009-08-27</date><risdate>2009</risdate><volume>27</volume><issue>39</issue><spage>5419</spage><epage>5426</epage><pages>5419-5426</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><eissn>0264-410X</eissn><coden>VACCDE</coden><abstract>Abstract We have designed chemically defined diepitope constructs consisting of liposomes displaying at their surface synthetic oligosaccharides mimicking the O-antigen of the Shigella flexneri 2a lipopolysaccharide (B-cell epitope) and influenza hemagglutinin peptide HA 307–319 (Th epitope). Using well controlled and high-yielding covalent bioconjugation reactions, the two structurally independent epitopes were coupled to the lipopeptide Pam3 CAG, i.e. a TLR2 ligand known for its adjuvant properties, anchored in preformed vesicles. The synthetic construct containing a pentadecasaccharide corresponding to three O-antigen repeating units triggered T-dependent anti-oligosaccharide and anti- S. flexneri 2a LPS antibody responses when administered i.m. to BALB/c mice. Moreover, the long-lasting anti-LPS antibody response afforded protection against a S. flexneri 2a challenge. These results show that liposome diepitope constructs could be attractive alternatives in the development of synthetic carbohydrate-based vaccines.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>19559116</pmid><doi>10.1016/j.vaccine.2009.06.031</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-5622-1422</orcidid><orcidid>https://orcid.org/0000-0002-8378-2508</orcidid></addata></record> |
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| ispartof | Vaccine, 2009-08, Vol.27 (39), p.5419-5426 |
| issn | 0264-410X 1873-2518 0264-410X |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); ProQuest Central UK/Ireland |
| subjects | Adjuvants Allergy and Immunology Animals Antibodies, Bacterial - blood Antibodies, Bacterial - immunology Applied microbiology Bacterial Vaccines - immunology Bacteriology Biological and medical sciences Dysentery, Bacillary - immunology Dysentery, Bacillary - prevention & control Epitopes, B-Lymphocyte - immunology Epitopes, T-Lymphocyte - immunology Female Fundamental and applied biological sciences. Psychology Hemagglutinins - immunology Immunology Life Sciences Lipopeptides - chemistry Lipopeptides - immunology Liposomes Liposomes - immunology Mice Mice, Inbred BALB C Microbiology Miscellaneous Molecular Structure O Antigens - chemistry O Antigens - immunology Oligosaccharides - chemistry Oligosaccharides - immunology Orthomyxoviridae Shigella flexneri Shigella flexneri - immunology Shigella flexneri 2a O-antigen Synthetic oligosaccharide-based vaccination construct Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, Synthetic - immunology |
| title | Rational design and immunogenicity of liposome-based diepitope constructs: Application to synthetic oligosaccharides mimicking the Shigella flexneri 2a O-antigen |
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