Second harmonic microscopy of axonemes

We performed Second Harmonic Microscopy of axonemes obtained from sea urchin sperm. Using polarization analysis and a trade-off between signal and photodamage, we were able to determine, for the first time to our knowledge, the nonlinear susceptibility chizxx/chixzx = 1.1+/-0.2 and chizzz/chixzx = 4...

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Veröffentlicht in:	Optics express 2009-05, Vol.17 (11), p.9235-9240
Hauptverfasser:	Odin, Christophe, Heichette, Claire, Chretien, Denis, Le Grand, Yann
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Axoneme - ultrastructure Biochemistry, Molecular Biology Biophysics Cells, Cultured Life Sciences Microscopy, Fluorescence - methods Microscopy, Phase-Contrast - methods Optics Physics Sea Urchins - ultrastructure
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Beschreibung
Zusammenfassung:	We performed Second Harmonic Microscopy of axonemes obtained from sea urchin sperm. Using polarization analysis and a trade-off between signal and photodamage, we were able to determine, for the first time to our knowledge, the nonlinear susceptibility chizxx/chixzx = 1.1+/-0.2 and chizzz/chixzx = 4+/-0.5 of axonemes.
ISSN:	1094-4087 1094-4087
DOI:	10.1364/OE.17.009235